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(11)

EP 2 225 558 A1

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(43) Date of publication: 08.09.2010 Bulletin 2010/36 (21) Application number: 08864122.0 (22) Date of filing: 11.12.2008

(51) International Patent Classification (IPC):  G01N 33/50(2006.01) C12Q 1/48(2006.01) (86) International application number: PCT/EP2008/010530 (87) International publication number: WO 2009/080219 (02.07.2009 Gazette 2009/27)

(84) Designated Contracting States: AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MT NL NO PL PT RO SE SI SK TR Designated Extension States: AL BA MK RS (30) Priority: 20.12.2007 US 15291 (71) Applicant: F. Hoffmann-La Roche AG 4070 Basel (CH)

(72) Inventors: BITTER, Hans, Marcus, Ludwig San Francisco California 94103 (US) GOLDSTEIN, David, Michael San Jose California 95120 (US) GONZALUDO, Nina Palo Alto California 94304 (US) KIRCHNER, Stephan 79540 Loerrach (DE) KOLAJA, Kyle, L. San Mateo California 94403 (US) OLAHARSKI, Andrew, James Sunnyvale California 94085 (US) (74) Representative: Brodbeck, Michel et al F. Hoffmann-La Roche AG Patent Department CLP Grenzacherstrasse 124 4070 Basel 4070 Basel (CH)