Oligonucleotide library encoding randomised peptides

(19)

(11)

EP 2 236 612 A8

(12)	CORRECTED EUROPEAN PATENT APPLICATION
	Note: Bibliography reflects the latest situation

(15)	Correction information:
	Corrected version no 1 (W1 A1)

(48)	Corrigendum issued on:
	30.11.2011 Bulletin 2011/48

(43)	Date of publication:
	06.10.2010 Bulletin 2010/40

(21)	Application number: 10166905.9

(22)	Date of filing: 18.07.2006

(51)

International Patent Classification (IPC):

C12N 15/66^(2006.01)

(84)	Designated Contracting States:
	AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR

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Priority:

22.07.2005 GB 0515131

(62)	Application number of the earlier application in accordance with Art. 76 EPC:
	06765007.7 / 1907548

(71)	Applicant: Aston University
	Birmingham, West Midlands B4 7ET (GB)

(72)	Inventors:
	Ashraf, Mohammed Birmingham, West Midlands B8 1NG (GB) Hughes, Marcus, Daniel Cradley Heath, West Midlands B64 5LU (GB) Hine, Anna, Victoria Kidderminster, Worcestershire DY10 1XB (GB)

(74)	Representative: Elsy, David
	Withers & Rogers LLP 4 More London Riverside London SE1 2AU London SE1 2AU (GB)


	Remarks:
	This application was filed on 22-06-2010 as a divisional application to the application mentioned under INID code 62.

(54)	Oligonucleotide library encoding randomised peptides

(57) The invention provides a kit for producing an oligonucleotide library, the library comprising a plurality of oligonucleotides, each oligonucleotide having at least one predetermined position, a randomisation codon selected from a defined group of codons, the coding within the defined group coding for different amino acids, the kit comprises a plurality of different randomisation oligonucleotides comprising:
(i) a coding strand, the coding strand comprising a randomisation codon; and
(ii) a substantially complementary non-coding strand,

wherein each double stranded randomisation oligonucleotide comprises a nucleotide sequence coding for a restriction endonuclease recognition site capable of being recognised by a restriction endonuclease, the restriction endonuclease capable of cleaving the randomisation oligonucleotide upstream or downstream of the endonuclease recognition site at a predetermined cleavage site to create a blunt ended cut.