Polynucleotide primers for detecting PIK3CA mutations

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EP 2 236 627 A8

(12)	CORRECTED EUROPEAN PATENT APPLICATION
	Note: Bibliography reflects the latest situation

(15)	Correction information:
	Corrected version no 1 (W1 A1)

(48)	Corrigendum issued on:
	01.12.2010 Bulletin 2010/48

(43)	Date of publication:
	06.10.2010 Bulletin 2010/40

(21)	Application number: 10158519.8

(22)	Date of filing: 29.09.2008

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International Patent Classification (IPC):

C12Q 1/68^(2006.01)

(84)	Designated Contracting States:
	AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MT NL NO PL PT RO SE SI SK TR

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Priority:

28.09.2007 GB 0719034

(62)	Application number of the earlier application in accordance with Art. 76 EPC:
	08806456.3 / 2205760

(71)	Applicant: DXS LIMITED
	Manchester M13 9XX (GB)

(72)	Inventors:
	Board, Ruth Manchester M20 4BX (GB) Ferguson, Jennifer Manchester MX13 9XX (GB) Ravetto, Paul Francis Manchester MX13 9XX (GB) Thelwell, Nicola Jo Manchester MX13 9XX (GB) Whitcombe, David Manchester MX13 9XX (GB)

(74)	Representative: Vossius & Partner
	Siebertstrasse 4 81675 München 81675 München (DE)


	Remarks:
	This application was filed on 30-03-2010 as a divisional application to the application mentioned under INID code 62.

(54)	Polynucleotide primers for detecting PIK3CA mutations

(57) A polynucleotide comprising at least the final six nucleotides of one of the following primer sequences, or a sequence complementary thereto: SEQ. ID NOS. 11 to 16, 18, or 37 to 39.
A method of detecting the presence or absence of a mutation in the PIK3CA gene, wherein the mutation is one of H1047R, H1047L, E542K and E545K, and preferably ARMS primers are combined with Scorpion primers.