(19)

(11)

EP 2 247 727 A2

(12)

(88) Date of publication A3: 21.01.2010 (43) Date of publication: 10.11.2010 Bulletin 2010/45 (21) Application number: 09711405.2 (22) Date of filing: 12.02.2009

(51) International Patent Classification (IPC):  C12N 15/10(2006.01) (86) International application number: PCT/US2009/033936 (87) International publication number: WO 2009/102878 (20.08.2009 Gazette 2009/34)

(84) Designated Contracting States: AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK TR Designated Extension States: AL BA RS (30) Priority: 12.02.2008 US 28146 21.03.2008 US 38696 23.06.2008 US 74991 01.08.2008 US 85811 (71) Applicant: Nugen Technologies, Inc. San Carlos, CA 94070 (US)	(72) Inventor: KURN, Nurith Palo Alto CA 94306 (US) (74) Representative: Roques, Sarah Elizabeth  J.A. Kemp & Co. 14 South Square Gray's Inn London WC1R 5JJ London WC1R 5JJ (GB)