The present application claims the priority of Chinese Patent Application No. 200810020387.1 filed on March 4, 2008 and entitled "A Composition Comprising 3-methyl-1-phenyl-2-pyrazolin-5-one", which is incorporated into herein by reference in its entirety.

The present invention relates to a pharmaceutical composition and the use thereof in the treatment of cerebrovascular diseases.

Cerebrovascular diseases (CVD) are a group of diseases which are severely harmful to human health and have currently become one of important factors for human disability and mortality.

Cerebrovascular disease refers to a brain disorder due to cerabrovascular abnormality. Stroke generally refers to acute cerebrovascular disease.

Cerebrovascular diseases can be simply divided into two classes: (1) ischemic cerebrovascular disease caused by reduced or blocked blood flow, and (2) hemorrhagic cerebrovascular disease caused by broken blood vessel. Ischemic cerebrovascular diseases are mainly manifested as cerebral infarctions (including cerebral thrombosis and cerebral embolism). Additionally, another manifestation of ischemic cerebrovascular disease is called transient ischemic attack (TIA, usually abbreviated by doctors), which can be completely recovered within 24 hours without any sequela. Hemorrhagic cerebrovascular diseases can be further divided into two classes: (1) cerebral hemorrhage, in which blood vessel is broken and the blood flows into cerebral parenchyma; and (2) subarachnoid hemorrhage (SAH, abbreviated by doctors), in which blood vessel is broken and the blood flows into subarachnoid space surrounding the brain.

3-methyl-1-phenyl-2-pyrazolin-5-one, also called Edaravone, has a structural formula of a molecular formula of C₁₀H₁₀N₂O and a molecular weight of 174.19.

3-methyl-1-phenyl-2-pyrazolin-5-one is a brain protective agent, which scavenges free radicals, inhibits lipid peroxidation, and thereby inhibits oxidative damages of brain cells, vascular endothelial cells and nerve cells. Intravenous administration of Edaravone into rats after ischemia/reperfusion suppresses the development of cerebral edema and cerebral infarction, relieves accompanying neurological symptoms and inhibits delayed neuronal death. It has been found that 3-methyl-1-phenyl-2-pyrazolin-5-one, with clinical dosage of 60 mg/day for an adult, has some side and adverse effects such as acute renal failure to uncertain extent, liver dysfunction, thrombocytopenia, diffuse intravascular thrombosis.

Borneol, commonly used in Chinese traditional medicine, is known as a resuscitation-inducing aromatic herbal agent and primes other drugs to the upper. Borneol is generally used as a priming agent to promote the efficacies of other drugs. It is indicated in Augmented Materia Medica (Bencao Yanyi) that borneol is poor when used alone and instead significant when used as an adjuvant.

An objective of the present invention is to provide a pharmaceutical

The use of Borneol for treating cerebrovascular disease has for example been disclosed in CN 1846740 and CN 1823922, which also disclose that Edavarone is useful for treating cerebrovascular diseases and is used as a reference compound for the comparative examples.

The use of Edavorone (as a high concentration low volume injection) for treating cerebrovascular diseases has for example been disclosed in EP 1 386 606 A1.

However, there is no disclosure of a combination of Borneol and Edavarone and its use in the treatment of cerebrovascular diseases.

composition comprising 3-methyl-1-phenyl-2-pyrazolin-5-one and borneol. The pharmaceutical composition in combination has synergistic effect and improved efficacies for the treatment of cerebrovascular diseases.

Preferably, the weight ratio of 3-methyl-1-phenyl-2-pyrazolin-5-one to borneol is from 4:1 to 1:4, and more preferably from 2:1 to 1:2.

Said borneol includes natural borneol and synthetic borneol, and natural borneol is preferably used. The natural borneol recited in Pharmacopoeia of People's Republic of China, 2005 Ed, that is dextro-camphol (LongNao), may be used.

The above pharmaceutical compositions may further comprise a solvent, which can facilitate the mixing of 3-methyl-1-phenyl-2-pyrazolin-5-one and borneol. The solvent may be selected from a group consisting of water-soluble organic solvent or a mixture of water-soluble organic solvent and water. Commonly used water-soluble organic solvents include but not limited to alcohol solvents, ether solvents and ketone solvents. Commonly used alcohol solvents include but not limited to ethanol, isopropanol, ethylene glycol and propylene glycol. Commonly used ether solvents include but not limited to ethylene glycol monoethyl ether and ethylene glycol monobutyl ether. Commonly used ketone solvents include but not limited to acetone and N-methyl-2-pyrrolidone. Preferably, the water-soluble organic solvent is propylene glycol.

Another objective of the present invention is to provide use of the above pharmaceutical composition in the treatment of cerebrovascular diseases.

Preferably, the above pharmaceutical composition is used in the treatment of ischemic cerebrovascular disease or cerebral infarction.

The pharmaceutical composition provided herein comprises 3-methyl-1-phenyl-2-pyrazolin-5-one and borneol, has synergistic effect in the treatment of cerebrovascular diseases and thereby has significantly improved efficacies. Accordingly, the pharmaceutical composition may have a reduced dosage 3-methyl-1-phenyl-2-pyrazolin-5-one and is still effective, and thereby has a reduced toxicity.

The present invention is further described with reference to the following examples. Edaravone recited in the examples is 3-methyl-1-phenyl-2-pyrazolin-5-one. Natural borneol recited is that recorded in Pharmacopoeia of People's Republic of China (2005 edition), namely dextro-camphol.

2 g of Edaravone is added to and completely dissolved by agitation in a solution of 200 g of propylene glycol. Then water for injection is added slowly and dissolves the mixture to a volume of 1000 mL.

2 g of synthetic borneol is added to and completely dissolved by agitation in a solution of 200 g of propylene glycol. Then water for injection is added slowly and dissolves the mixture to a volume of 1000 mL.

2 g of natural borneol is added to and completely dissolved by agitation in a solution of 200 g of propylene glycol. Then water for injection is added slowly and dissolves the mixture to a volume of 1000 mL.

2 g of Edaravone and 1 g of synthetic borneol are added to and completely dissolved by agitation in a solution of 200 g of propylene glycol. Then water for injection is added slowly and dissolves the mixture to a volume of 1000 mL.

2 g of Edaravone and 1 g of natural borneol are added to and completely dissolved by agitation in a solution of 200 g of propylene glycol. Then water for injection is added slowly and dissolves the mixture to a volume of 1000 mL.

1 g of Edaravone and 2 g of natural borneol are added to and completely dissolved by agitation in a solution of 100 g of propylene glycol. Then water for injection is added slowly and dissolves the mixture to a volume of 1000 mL.

4 g of Edaravone and 1 g of natural borneol are added to and completely dissolved by agitation in a solution of 400 g of propylene glycol. Then water for injection is added slowly and dissolves the mixture to a volume of 1000 mL.

1 g of Edaravone and 1 g of natural borneol are added to and completely dissolved by agitation in a solution of 200 g of propylene glycol. Then water for injection is added slowly and dissolves the mixture to a volume of 1000 mL.

1 g of Edaravone and 2 g of natural borneol are added to and completely dissolved by agitation in a solution of 200 g of propylene glycol. Then water for injection is added slowly and dissolves the mixture to a volume of 1000 mL.

2 g of Edaravone and 0.5 g of natural borneol are added to and completely dissolved by agitation in a solution of 200 g of propylene glycol. Then water for injection is added slowly and dissolves the mixture to a volume of 1000 mL.

4.5 g of Edaravone and 0.5 g of natural borneol are added to and completely dissolved by agitation in a solution of 400 g of propylene glycol. Then water for injection is added slowly and dissolves the mixture to a volume of 1000 mL.

2 g of Edaravone and 1 g of natural borneol are added to and completely dissolved by agitation in a solution of 100 g of propylene glycol. Then water for injection is added slowly and dissolves the mixture to a volume of 1000 mL.

8 g of Edaravone and 4 g of natural borneol are added to and completely dissolved by agitation in a solution of 500 g of propylene glycol. Then water for injection is added slowly and dissolves the mixture to a volume of 1000 mL.

2 g of Edaravone is added to and completely dissolved by agitation in a solution of 100 g of propylene glycol. Then water for injection is added slowly and dissolves the mixture to a volume of 1000 mL.

8 g of Edaravone is added to and completely dissolved by agitation in a solution of 400 g of propylene glycol. Then water for injection is added slowly and dissolves the mixture to a volume of 1000 mL.

Examples 16-19 are examples for comparison of efficacies.

Main steps are described as follows. Rats were anesthetized with 10% chloral hydrate (350mg/kg, i.p.). Right external carotid was separated, ligated and severed. A nylon thread with rounded tip was inserted slowly for about 18 mm in depth into the residual end of right external carotid and through common carotid and internal carotid to induce ischemia by blocking the origin of middle cerebral artery. After ischemia for 2h, the nylon thread was removed for reperfusion for 24h. Animals in the sham group were treated identically to those in the model group, except that a nylon thread was just used to separate the artery. Manifestation of Homer's symptom and opposite lateral body dyskinesia after animal revival means a successful modelling.

Experimental animals were randomly divided into five groups, including sham group, control group and drug groups. Animals in drug groups were intraperitoneally injected twice, 30 min before ischemia and 12 h after reperfusion. Animals in sham group and control group were each injected with equal volume of normal saline instead.

Neurological deficit scoring: Behavioral evaluation was performed 24 h after reperfusion according to Longa 5-point scoring system.

Determination of cerebral infarction size: Animals were decapitated to get the brain after the last neurological deficit scoring. Rhinencephalon, lower brain stem and cerebella were removed, and the remaining brains were immediately weighted to obtain wet weights. The brains were sectioned coronally into five slices with substantially identical thickness on ice, then incubated in 2,3,5-triphenyl tetrazolium chloride for 30 min at 37° C. Normal brain tissues appeared rose, while infarction areas appeared white. Next, brain slices were fixed in 10% formaldehyde, and white tissues were carefully removed and weighed. Infarction size was determined by the percentage of infracted tissue weight in the total cerebral weight.

Determination of cerebral water content: Cerebral water contents were determined by dry and wet method. Animals were sacrificed to get the whole brain. Rhinencephalon, lower brain stem and cerebella were removed and the wet weight of the remaining brains was determined immediately. Dry weight was obtained after baking in a oven for about 18h at 120° C. Cerebral water content was calculated according to the following equation: Cerebral water content (%) = (cerebral wet weight - cerebral dry weight) / cerebral wetweight X 100.

The infarction size after ischemia reperfusion was significantly reduced in rats of all drug groups (P<0.01) compared with those of control group. Neither Edaravone alone nor natural borneol alone had significant effect on symptoms of neurological deficits in rats. However, the combination of Edaravone and natural borneol significantly ameliorated symptoms of neurological deficits, indicating that the two drugs have synergistic effect. Results were shown in table 1. Group Cerebral infarction size (%) neurological deficit score (points) control 35.7±7.52 3.1±1.05 Edaravone (1.0 mg.kg^-1) 18.0±5.29** 2.5±1.02 Natural borneol (0.5 mg-kg^-1) 19.8±6.37** 2.0±0.98 Combination (0.5 mg·kg^-1 Edaravone + 0.25 mg·kg^-1 Natural borneol) 18.1±3.61** 1.1±0.69* * P<0.05, ** P<0.01, compared with control group.

Cerebral edema induced by ischemia/reperfusion was significantly reduced in rats of all drug groups. Both Edaravone alone and natural borneol alone had significant effect on cerebral water content compared with controll group (P<0.05). The combination of Edaravone and natural borneol had highly significant effect on cerebral water content compared with controll group (P<0.01), indicating that the two drugs have synergistic effect. Results were shown in Table 2. Group Cerebral water content (%) Sham 78.41±1.15 control 82.19±1.07 Edaravone (1.0 mg·kg^-1) 81.65±1.24* Natural borneol (0.5 mg·kg^-1) 81.25±0.97* Combination (0.5 mg·kg^-1 Edaravone + 0.25 mg·kg^-1 natural borneol) 80.12±1.43** * P<0.05, ** P<0.01, compared with control group.

Middle cerebral artery occlusion (MCAO), a cerebral ischemia/reperfusion model, was prepared using the internal carotid thread method. Animals were anesthetized with 10% chloral hydrate (3.5 mL/kg), and then fixed on the operation table in prone position. The skin was disinfected and the neck was incised from the midline. Right common carotid, external carotid and internal carotid were separated, and vagus nerve was separated carefully. The external carotid was ligated and severed. Moved forward along the internal carotid, and then pterygopalatine artery was ligated. The proximal part of common carotid was clamped and a cut was made at the distal end of the external carotid ligature. A nylon thread with the outer diameter of 0.285 mm was inserted into the cut, through the branch of common carotid and then into internal carotid slowly until a slight resistance was met (about 20 mm distance from the divergence point). Blood supply in middle cerebral artery was thereby completely blocked. Cerebral blood flow was monitored by using laser Doppler cerebral flowmetry. After ischemia in the right brain for 2.0 h, the nylon thread was withdrawn slowly and then blood supply was recovered for reperfusion. The scalp was sutured and disinfected. In the sham group, only the blood vessels were separated without the insertion of a nylon thread. During the operation, rat heating plate and desk lamp with 60W were used to keep the body temperature of rats at 37.0±0.5° C.

Experimental animals were randomly divided into six groups, including sham group, model group, positive control group and drug groups (the total dosage of Edaravone + natural borneol was 0.75 mg/kg). After cerebral ischemia was modeled in experimental animals, all animals were divided into the groups in a single-blind manner with identical probability. Respective drugs were administrated once immediately after cerebral ischemia, and then administrated sequentially after 2, 4 and 24 hours. The four administrations were made totally to each animal. Rats in model group were injected through tail vein with equal volume of normal saline. Rats in sham group were injected through tail vein with equal volume of normal saline. Rats in positive control group were administrated with Nimodipine injection. The dosage in positive control group was 1.2 mg/kg per animal for three dosages and a single dosage was 0.4 mg/kg each animal.

Neurological deficit scoring: neurological deficit symptoms were evaluated according to modified Bederson scoring system of 5-point. Neurological deficit symptoms after cerebral trauma in rats were evaluated by using single-blinded method. That is, the experiment designer labeled the animals in groups, while the experiment performer who scored neurological deficit symptoms were blinded for animals grouping. After the evaluation was finished, the scorer presented the scoring result of the labeled animals to the designer. The designer unblinded the experiment and obtained the score of each animal in respective groups.

Neurological Deficit Scoring: the detail criterion of Bederson 5-point scoring system is:

• 0: Both forelimbs of the animal extend toward the floor when the animal keeps suspended by holding the animal's tail, and no other behavioral defect is observed.
• 1: The animal on its (left) forelimb opposite to the injured side shows wrist and elbow flexion, shoulder adduction, elbow abduction and close attachment to the chest wall.
• 2: When placing the animal on a smooth plate and pushing the shoulder on the injured side toward the opposite side, a reduced resistance is met.
• 3: When walks freely, the animal makes circular movement opposite to the injured side.
• 4: The animal shows flaccid and paralyzed limbs and has no active limb movement.

Determination of cerebral infarction area and brain lesion: The animal was anesthetized with 10% chloral hydrate and decapitated to get the brain. After Rhinencephalon, cerebella and lower brain stem were removed, blood on the brain surface was washed with normal saline and residual water on the surface were blotted up. After being placed at -80° C for 7 min, the brain was taken out and immediately coronal section was vertically and downward made from the optic chiasma, and thereby the brain was cut into slices backwards every 2 mm. Brain slices were immersed in 20 g/L TTC staining solution (37° C,90 min) formulated freshly in 0.2 mol/L PBS pH 7.4-7.8. Normal brain tissues were stained crimson, while ischemic brain tissues appeared white. After rinsed with normal saline, brain slices were arranged in a row sequentially and rapidly. Residual water on the surface were blotted up and the slices were photographed. The left and right brain tissues were separated and weighed respectively as W_left (weight of left brain) and W_right (weight of right brain). 15 mL freshly formulated exaction solution (DMSO (dimethyl sulfoxide) : ethanol = 1:1) was added to left and right brain tissues respectively. The tissues were extracted at 25 ° C for 24 hours in dark. The generated red substance, formazan, was extracted sufficiently until the brain slices turned white.

1. (1) analysis of the photos were made by using image analysis software. Right ischemia area (white) and right total area were marked and the percentage of infarction area was calculated by the following formula: $$\mathrm{Cerebral\; infraction\; area}\%=100X\frac{\mathrm{Total\; ischemia\; area}}{\mathrm{Right\; total\; area}}$$
2. (2) The absorbance of the respective exacts (extract 100 µL + extract solution 1900 µL) was determined at 485 nm and was averaged from four independent determinations to produce A_left and A_right. The percentage of brain lesion was calculated by the following formula: $$\mathrm{brain\; lesion}\mathrm{\%}\mathrm{=}\mathrm{100}\mathrm{X}\left(\mathrm{1}\mathrm{-}\frac{{\mathrm{A}}_{\mathrm{right}}\mathrm{X}{\mathrm{W}}_{\mathrm{left}}}{{\mathrm{A}}_{\mathrm{left}}\mathrm{X}{\mathrm{W}}_{\mathrm{right}}}\right)$$

Quantitative data were expressed in mean ± SD. Cerebral infarction area and neurological deficit score were evaluated by one-way ANOVA. The significance of difference between two groups was determined by Scheffe's test. Animal mortality was compared between groups by X² test. P<0.05 was defined as significant difference.

The score of neurological deficit symptoms were shown in Table 3. Compared with model group, formulations of Edaravone and natural borneol in various ratios and Nimodipine significantly ameliorated neurological deficit symptoms (F_4,35 = 14.59, P = 0.000). It was shown that Edaravone and natural borneol in combination can ameliorate neurological deficit symptoms significantly. Group Model Nimodipine A:B=2:1 A:B=1:1 A:B=1:2 mean ± SD 2.6±0.52 1.1±0.58 * 0.81±0.26* 1.1±0.35 * 1.5±0.76 * X±SD, n=8; * P<0.05, compared with model group.

Cerebral infarction areas in various groups were shown in Table 4. Compared with model group, treatment by Edaravone and natural borneol in ratios of 2:1 and 1:1, and by Nimodipine significantly reduced cerebral infarction area (F_4,35 = 5.38, P = 0.002). Compared with model group, treatment by Edaravone and natural borneol in a ratio of 1:2 has the tendency to reduce cerebral infarction area (F₄,₃₅ = 5.38, P = 0.358). Group Model Nimodipine A:B=2:1 A:B=1:1 A:B=1:2 mean ± SD 27.4±13.4 8.8±4.0 * 9.4±4.9 * 10.8±5.0 * 17.2±14.7 * X±SD, n=8; * P<0.05, compared with model group.

Brain lesion in various groups were shown in Table 5. Compared with model group, treatment by Edaravone and natural borneol in ratios of 2:1 and 1:1, and by Nimodipine significantly reduced the severity of brain lesion (F_4,35 = 5.36, P = 0.002). Compared with model group, treatment by Edaravone and natural borneol in a ratio of 1:2 has the tendency to reduce the severity of brain lesion (F_4,35 = 5.36, P = 0.239). Group Model Nimodipine A:B=2:1 A:B=1:1 A:B=1:2 mean ± SD 33.2±14.8 11.6±5.0 * 11.7±6.4 * 11.1±6.8 * 19.3±18.2 * X±SD, n=8; * P<0.05, compared with model group.

Experimental methods and evaluation methods are the same as Example 13.

The score of neurological deficit symptoms were shown in Table 6. Compared with model group, treatment by Edaravone and natural borneol in various ratios, and by Nimodipine significantly ameliorated neurological deficit symptoms (F_4,35 = 10.31, P = 0.000). It was shown that Edaravone and natural borneol in combination can ameliorate neurological deficit symptoms significantly. Group Model Edaravone (3mglkg) A:B=2:1 A:B=4:1 A:B=9:1 Mean± SD 2.7±0.5 0.94±0.46 * 1.1±0.54 * 1.3±0.87 * 1.6±0.55 * X±SD, n=8; * P<0.05, compared with model group.

Cerebral infarction areas in various groups were shown in Table 7. Compared with model group, treatment by Edaravone and natural borneol in a ratio of 2:1, and by Edaravone 3mg/kg significantly reduced cerebral infarction area (F_4,35 = 4.62, P = 0.03). Compared with model group, treatment by Edaravone and natural borneol in ratios of 4:1 and 9:1 had the tendency to reduce cerebral infarction area (F_4,35 = 4.62, P = 0.081). Group Model Edaravone (3mg/kg) A:B=2:1 A:B=4:1 A:B=9:1 Mean± SD 25.4±14.8 9.7±4.6 * 10.3±5.3 * 14.6±10.9 16.5±11.0 X±SD, n=8; * P<0.05, compared with model group.

Brain lesions in various groups were shown in Table 8. Compared with model group, treatment by Edaravone and natural borneol in a ratio of 2:1 , and by Edaravone 3mg/kg significantly reduced the severity of brain lesion (F_4,35 = 5.78, P = 0.04). Compared with model group, treatment by Edaravone and natural borneol in ratios of 4:1 and 9:1 had the tendency to reduce the severity of brain lesion (F_4,35 = 5.78, P = 0.159). Group Model Edaravone (3mg/kg) A:B=2:1 A:B=4:1 A:B=9:1 Mean± SD 32.2±15.1 11.6±9.7 * 13.1±8.3 * 22.3±17.7 23.9±19.8 X±SD, n=8; * P<0.05, compared with model group.

Solvent 0.9% sodium chloride injection (by weight) 10mL/kg vehicle 40% propylene glycol solution (by volume) 10mL/kg Edaravone 80mg/kg (propylene glycol content: 40%) Edaravone 20mg/kg (propylene glycol content: 10%) Combined Edaravone 120mg/kg (propylene glycol content: 50%) Combined Edaravone 30mg/kg (propylene glycol content: 10%)

Combined Edaravone in this Example refers to Edaravone+natural borneol (weight ratio = 2:1).

In the above dosage groups, the dosage per day per rabbit was calculated according to actual weight of each rabbit. The test drugs were administrated in equal volume according to body weight of respective rabbits.

36 rabbits were numbered and randomly divided into six groups according to the body weight, including 4 dosage groups, "Edaravone + natural borneol (weight ratio=2:1)" 120mg/kg, 30mg/kg; "Edaravone" 80mg/kg, 20mg/kg; as well as vehicle group (40% propylene glycol solution) and solvent group (0.9% sodium chloride injection). There were 6 rabbits in each group, including 3 males and 3 females each. Rabbits were habituated and observed for 3 days before the test. During the test, rabbits were dosed once at the same time in each morning via marginal ear vein injection. Rabbits in high dosage groups were continuously dosed for 13 days, while in low dosage groups, solvent group and vehicle group for 20 days. The dosage were regulated in accordance with body weight variation. Animal responses, stimulations at injection sites and histopathological examination in kidneys and injection site were used to comprehensively evaluate the toxicity of either combined Edaravone or Edaravone on the rabbits.

Hematuria: During the continuous intravenous administration" temporary hematuria occurred 30 min to 2 hr after the administration in groups treated with Edaravone 80mg/kg or 20mg/kg, combined Edaravone 120mg/kg or 30mg/kg, or 40% propylene glycol solution 10mL/kg. The incidence of hematuria was 100% in Edaravone 80mg/kg group, 100% in Edaravone 20mg/kg group, 100% in combined Edaravone 120mg/kg group, 50% in combined Edaravone 30mg/kg group, 100% in 40% propylene glycol solution 10mL/kg group. However, no hematuria occurred in 0.9% sodium chloride injection group.

Gross necropsy and histopathological examinations: 1) gross necropsy: In Edaravone 80mg/kg group, 3 of 6 rabbits had khaki and swollen kidney having uneven surface, one of the rabbits suffered hepatonecrosis and had a khaki and hard liver. In Edaravone 20mg/kg group, 3 of 6 rabbits had a swollen kidney. In combined Edaravone 120mg/kg group, 2 of 6 rabbits had a light-colored and swollen kidney. No obvious changes of kidney were observed in combined Edaravone 30mg/kg group and 40% propylene glycol solution 10mL/kg group. 2) Histopathological examination: In Edaravone 80mg/kg and 20mg/kg groups and combined Edaravone 120mg/kg and 30mg/kg groups, stimulatory changes such as vascular occlusion to varied extents were observed at injection sites. In the above groups, rabbit's kidney lesions were also observed, main manifestations of which were progressive renal lesion such as renal tubular lesion, inflammatory infiltration of mesenchyme, and glomerular lesion. In 40% propylene glycol solution 10mL/kg group, stimulatory changes such as vascular occlusion also occurred at injection sites, while only mild renal tubular swelling and vacuolar degeneration were observed from their kidneys.

It was concluded from the above experimental results that, in the above dosage conditions, 1: Edaravone and combined Edaravone show substantially similar stimulatory effects on the injection sites of rabbits. 2: Edaravone induces more incidence of hematuria in rabbits than combined Edaravone. 3: Edaravone has higher toxicity on rabbit's kidney than combined Edaravone. Only some preferred embodiments of the present invention are described as above. It should be noted that the skilled in the art can make further improvements and/or modifications without departing from the spirits of the present invention, which are still claimed in the present invention.