| (19) |
 |
|
(11) |
EP 2 275 431 A3 |
| (12) |
EUROPEAN PATENT APPLICATION |
| (88) |
Date of publication A3: |
|
25.05.2011 Bulletin 2011/21 |
| (43) |
Date of publication A2: |
|
19.01.2011 Bulletin 2011/03 |
| (22) |
Date of filing: 16.01.2009 |
|
| (51) |
International Patent Classification (IPC):
|
|
| (84) |
Designated Contracting States: |
|
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO
PL PT RO SE SI SK TR |
|
Designated Extension States: |
|
AL BA RS |
| (30) |
Priority: |
24.07.2008 US 135787 P
28.01.2008 US 62663 P
18.01.2008 US 11513 P
19.03.2008 US 69859 P
27.03.2008 US 70841 P
20.08.2008 US 189467 P
|
| (62) |
Application number of the earlier application in accordance with Art. 76 EPC: |
|
09702238.8 / 2238154 |
| (71) |
Applicant: BIO-RAD LABORATORIES, INC. |
|
Hercules, CA 94547-1803 (US) |
|
| (72) |
Inventor: |
|
- Gagnon, Peter S.
San Clemente, CA 92672 (US)
|
| (74) |
Representative: von Kreisler Selting Werner |
|
Deichmannhaus am Dom
Bahnhofsvorplatz 1
50667 Köln
50667 Köln (DE) |
|
| |
|
| (54) |
Enhanced fractionation of phosphorylated and nonphosphorylated biomolecules by apatite
chromotography |
(57) Methods are disclosed for the use of apatite chromatography, particularly without
reliance upon phosphate gradients, for fractionation or separation of phosphorylated
and nonphosphorylated biomolecules. Integration of such methods into multi-step procedures,
with other fractionation methods are additionally disclosed.