Pichia pastoris as a host for the production of the ribosome-inactivating protein (RIP) saporin and saporin fusion chimaeras

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EP 2 280 063 A8

(12)	CORRECTED EUROPEAN PATENT APPLICATION
	Note: Bibliography reflects the latest situation

(15)	Correction information:
	Corrected version no 1 (W1 A1)

(48)	Corrigendum issued on:
	23.03.2011 Bulletin 2011/12

(43)	Date of publication:
	02.02.2011 Bulletin 2011/05

(21)	Application number: 09166640.4

(22)	Date of filing: 28.07.2009

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International Patent Classification (IPC):

C12N 1/16^(2006.01)
C12P 21/02^(2006.01)
C07K 16/28^(2006.01)

C12N 15/62^(2006.01)
C07K 14/415^(2006.01)

(84)	Designated Contracting States:
	AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR
	Designated Extension States:
	AL BA RS

(71)	Applicants:
	Consiglio Nazionale delle Ricerche 00185 Roma (IT) Leukaemia Busters Southampton, Hampshire SO16 6YD (GB) Universita' Degli Studi Di L'Aquila 67100 L'Aquila (IT)

(72)	Inventors:
	Ceriotti, Aldo 00185 Roma (IT) Della Cristina, Pietro 00185 Roma (IT) Colombatti, Marco 00185 Roma (IT) Fabbrini, M. Serena 00185 Roma (IT) Flavell, Sopsamorn U. Southampton, Hampshire SO16 6YD (GB) Flavell, David J. Southampton, Hampshire SO16 6YD (GB) Ippoliti, Rodolfo, Università degli Studi Aquila 67010 L'Aquila (IT) Lombardi, Alessio 00185 Roma (IT)

(74)	Representative: Minoja, Fabrizio
	Bianchetti Bracco Minoja S.r.l. Via Plinio 63 20129 Milano 20129 Milano (IT)

(54)	Pichia pastoris as a host for the production of the ribosome-inactivating protein (RIP) saporin and saporin fusion chimaeras

(57) The present invention relates to the field of recombinant protein expression and discloses a procedure for the production of toxic chimaeric proteins endowed with ribosome-inactivating activity based on the plant toxin Saporin from Saponaria officinalis in the methylotrophic yeast Pichia pastoris.