Kits for displacement Sandwich Immuno-PCR

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(11)

EP 2 290 100 A8

(12)	CORRECTED EUROPEAN PATENT APPLICATION
	Note: Bibliography reflects the latest situation

(15)	Correction information:
	Corrected version no 1 (W1 A3)

(48)	Corrigendum issued on:
	28.08.2013 Bulletin 2013/35

(88)	Date of publication A3:
	25.05.2011 Bulletin 2011/21

(43)	Date of publication:
	02.03.2011 Bulletin 2011/09

(21)	Application number: 10184768.9

(22)	Date of filing: 03.11.2003

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International Patent Classification (IPC):

C12Q 1/68^(2006.01)
G01N 33/536^(2006.01)
G01N 33/543^(2006.01)

G01N 33/53^(2006.01)
G01N 33/537^(2006.01)

(84)	Designated Contracting States:
	AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR

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Priority:

01.11.2002 US 423173 P

(62)	Application number of the earlier application in accordance with Art. 76 EPC:
	03810829.6 / 1563100

(71)	Applicant: Iris Molecular Diagnostics, Inc.
	Chatsworth CA 91311 (US)

(72)	Inventors:
	Jablonski, Ed Carlsbad, CA 92011 (US) Driver, David Carlsbad, CA 92011 (US) Adams, Tom Carlsbad, CA 92011 (US)

(74)	Representative: Viering, Jentschura & Partner
	Kennedydamm 55 / Roßstrasse 40476 Düsseldorf 40476 Düsseldorf (DE)

(54)	Kits for displacement Sandwich Immuno-PCR

(57) The present invention is directed to labeling antibodies with DNA in a convenient kit format for the purpose of producing and utilizing nucleic acid detection immunoassays. The process comprises treating a reporter antibody with a chemicaly activated strand of DNA, which serves as a template for any DNA amplification system. The reporter antibody-DNA conjugate is used to form a complex with corresponding antigen. The complex can be detected by the production of multiple copies of the DNA template. The kit may also provide an activated DNA sequence to label a capture antibody. The DNA conjugated to the capture antibody is modified to contain a high affinity binding ligand for the purpose of coating a solid support. The coated solid support functions to capture the immune complex containing reporter antibody in a sandwich assay format.; The DNA sequence of the capture antibody may also have properties providing for the detachment of the complex from the solid support.