(19)
(11) EP 2 294 225 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
18.02.2015 Bulletin 2015/08

(45) Mention of the grant of the patent:
17.12.2014 Bulletin 2014/51

(21) Application number: 09774388.4

(22) Date of filing: 30.06.2009
(51) International Patent Classification (IPC): 
C12Q 1/68(2006.01)
(86) International application number:
PCT/US2009/049323
(87) International publication number:
WO 2010/002938 (07.01.2010 Gazette 2010/01)

(54)

METHOD FOR DIRECT AMPLIFICATION FROM CRUDE NUCLEIC ACID SAMPLES

VERFAHREN ZUR DIREKTEN AMPLIFIKATION AUS ROHEN NUKEINSÄUREPROBEN

PROCÉDÉ D'AMPLIFICATION DIRECTE À PARTIR D'ÉCHANTILLONS BRUTS D'ACIDES NUCLÉIQUES


(84) Designated Contracting States:
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK TR

(30) Priority: 30.06.2008 US 77113 P

(43) Date of publication of application:
16.03.2011 Bulletin 2011/11

(60) Divisional application:
14197738.9

(73) Proprietor: Life Technologies Corporation
Carlsbad, CA 92008 (US)

(72) Inventors:
  • CHANG, Chien-wei
    Belmont, CA 94002 (US)
  • WANG, Dennis, Y.
    Dublin CA 94568 (US)
  • HENNESSY, Lori, K.
    San Mateo CA 94403 (US)

(74) Representative: Grund, Martin 
Grund Intellectual Property Group Postfach 44 05 16
80754 München
80754 München (DE)


(56) References cited: : 
EP-B1- 1 050 587
WO-A2-00/50564
US-A- 5 837 453
US-A1- 2005 026 147
US-A1- 2008 138 804
WO-A1-2006/090987
WO-A2-2008/002740
US-A1- 2002 012 924
US-A1- 2008 124 768
US-B1- 6 297 005
   
  • NAGAI M ET AL: "ADDITIVE EFFECTS OF BOVINE SERUM ALBUMIN, DITHIOTHREITOL, AND GLYCEROL ON PCR", BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL, ACADEMIC PRESS, LONDON, GB, vol. 44, no. 1, 1 January 1998 (1998-01-01), pages 157-163, XP009023284, ISSN: 1039-9712
  • ABU AL-SOUD WALEED ET AL: "Effects of amplification facilitators on diagnostic PCR in the presence of blood, feces, and meat", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 38, no. 12, December 2001 (2001-12), pages 4463-4470, ISSN: 0095-1137
   
Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).