Use of SNS-595 for treating leukemia

Description

[0001] SNS-595 is novel naphthyridine cytotoxic agent that was previously known as AG-7352 (see e.g., Tsuzuki et al, Tetrahedron-Asymmetry 12: 1793-1799 (2001) and U.S. Patent No. 5,817,669). The chemical name of SNS-595 is (+)-1,4-dihydro-7-[(3S,4S)-3-methoxy-4-(methylamino)-1-pyrrolidinyl]-4-oxo-1-(2-thiazoyl)-1,8-naphthyridine-3-carboxylic acid and has the structure shown below

[0002] The present invention relates to SNS-595 for use in a method of treating leukemia in a patient, wherein the method comprises administering SNS-595 to the patient in a dose of 60 mg/m2 to 150 mg/m2.

DESCRIPTION OF THE FIGURES

[0003] Figure 1 depicts the plasma concentrations of SNS-595 over time among the various patient cohorts.

DETAILED DESCRIPTION

[0004] The application herewith discloses a pharmaceutical composition comprising:

a) SNS-595 and

b) an acid

[0005] in an aqueous solution wherein the pH of the solution is 2-3.5. As used herein, a numerical range is intended to be inclusive. For example, the range of pH 2-3.5 includes both pH 2 and pH 3.5. In one embodiment, the pH of the composition is 2-3. In another embodiment, the pH of the composition is 2.3-2.7. As used herein, an aqueous solution is a liquid comprising water.

[0006] According to the invention, SNS-595 may be administered in form of such a pharmaceutical composition.

[0007] Suitable examples of acids include both organic and inorganic acids such as acetic acid, ascorbic acid, benzene-sulfonic acid, ethanesulfonic acid, glycolic acid, hydrogen chloride, hydrogen bromide, hydroxyethanesulfonic acid, lactic acid, maleic acid, methanesulfonic acid, proprionic acid, succinic acid, sulfuric acid, trifluoroacetic acid, and toluenesulfonic acid. In one embodiment, the acid is hydrochloric acid, methanesulfonic acid or lactic acid. In another embodiment, the acid is methanesulfonic acid.

[0008] The pharmaceutical composition may further comprises a tonicity agent. Suitable examples of a tonicity agent include amino acids (e.g., alanine and glycine), electrolytes (e.g., sodium chloride and potassium chloride), monosaccharides (e.g. glucose or galactose), disaccharides (e.g. sucrose) and hexahydric alcohols (e.g., mannitol and sorbitol). In another embodiment, the tonicity agent is sodium chloride, glucose, mannitol, or sorbitol. In another embodiment, the tonicity agent is a hexahydric alcohol. In another embodiment, the tonicity agent is sorbitol.

[0009] SNS-595 is a cytotoxic agent for the treatment of cancer. The types of cancers that can be treated using SNS-595 include but are not limited to: bladder cancer, breast cancer, cervical cancer, colon cancer (including colorectal cancer), esophageal cancer, head and neck cancer, leukemia, liver cancer, lung cancer (both small cell and non-small cell), lymphoma, melanoma, myeloma, neuroblastoma, ovarian cancer, pancreatic cancer, prostate cancer, renal cancer, sarcoma (including osteosarcoma), skin cancer (including squamous cell carcinoma), stomach cancer, testicular cancer, thyroid cancer, and uterine cancer.

[0010] The invention provides a method of using SNS-595 to treat leukemia. The method comprises administering to a patient on the basis of body surface area, a dose of 60 mg/m²-150 mg/m² of SNS-595. Body surface area calculations can be calculated for example, with the Mosteller formula wherein:

[0011] In another embodiment the dose is 60 mg/m²-70 mg/m². In another embodiment the dose is 65 mg/m²-75 mg/m². In another embodiment the dose is 70 mg/m²-80 mg/m². In another embodiment the dose is 75 mg/m²- 85mg/m². In another embodiment the dose is 80 mg/m²-90 mg/m². In another embodiment the dose is 85 mg/m²-95 mg/m². In another embodiment the dose is 90 mg/m²-100 mg/m².

[0012] In another embodiment the dose is 95 mg/ m²-105 mg/ m². In another embodiment the dose is 100 mg/ m²-1 10 mg/ m². In another embodiment the dose is 105 mg/ m²-115 mg/m². In another embodiment the dose is 110 mg/m²-120 mg/m². In another embodiment the dose is 115 mg/m²- 125 mg/m². In another embodiment the dose is 120 mg/m²-130 mg/m². In another embodiment the dose is 125 mg/m²-135 mg/m². In another embodiment the dose is 130 mg/m²- 140 mg/m². In another embodiment the dose is 135 mg/m²-145 mg/m². In another embodiment the dose is 140 mg/m²-150 mg/m².

[0013] The administered dose of SNS-595 can be delivered simultaneously (e.g. a single bolus injection) or over a 24-hour period (e.g., continuous infusion over time or divided bolus doses over time) and is repeated until the patient experiences stable disease or regression, or until the patient experiences disease progression or unacceptable toxicity. For example, stable disease for solid tumors generally means that the perpendicular diameter of measurable lesions has not increased by 25% or more from the last measurement. See e.g., Response Evaluation Criteria in Solid Tumors (RECIST) Guidelines, Journal of the National Cancer Institute 92(3): 205-216 (2000). Stable disease or lack thereof is determined by methods known in the art such as evaluation of patient symptoms, physical examination, visualization of the tumor that has been imaged using X-ray, CAT, PET, or MRI scan and other commonly accepted evaluation modalities.

[0014] The administered dose of SNS-595 can be expressed in units other than as mg/m². For example, doses can be expressed as mg/kg. One of ordinary skill in the art would readily know how to convert doses from mg/m² to mg/kg to given either the height or weight of a subject or both (see e.g., http:///www.fda.gov/cder/cancer/animalframe.htm). For example, a dose of 10 mg/m²-150 mg/m² for a 65 kg human is approximately equal to 0.26 mg/kg-3.95-mg/kg.

[0015] In another aspect of the present invention, SNS-595 is administered according to a dosing schedule. In one embodiment, the method comprises:

i) administering a dose of 60 mg/m²-150 mg/m² of SNS-595 to a patient;

ii) waiting a period of at least two days where the subject is not administered any SNS-595;

iii) administering another dose of 60 mg/m²-150 mg/m² of SNS-595 to the patient; and,

repeating steps ii)-iii) a plurality of times.

[0016] For example, if the waiting period were 6 days, then the initial dose of SNS-595 is administered on Day 1 (step i); the waiting period is six days (step ii); and the following dose of SNS-595 is administered on Day 8 (step iii). Other exemplary time periods include 2 days, 3 days, 13 days, 20 days, and 27 days. In another embodiment, the waiting period is at least 2 days and steps ii) through iii) are repeated at least three times. In another embodiment, the waiting period is at least 3 days and steps ii) through iii) are repeated at least five times. In another embodiment, the waiting period is at least 3 days and steps ii) through iii) are repeated at least three times. In another embodiment, the waiting period is at least 3 days and steps ii) through iii) are repeated at least five times. In another embodiment, the waiting period is at least 6 days and steps ii) through iii) are repeated at least three times. In another embodiment, the waiting period is at least 6 days and steps ii) through iii) are repeated at least five times. In another embodiment, the waiting period is at least 20 days and steps ii) through iii) are repeated at least three times. In another embodiment, the waiting period is at least 20 days and steps ii) through iii) are repeated at least five times. In another embodiment, the waiting period is at least 27 days and steps ii) through iii) are repeated at least three times. In another embodiment, the waiting period is at least 27 days and steps ii) through iii) are repeated at least five times.

[0017] In another embodiment, the dosing method comprises administering a weekly dose of SNS-595 to a subject. In another embodiment, the dosing method comprises administering a dose of SNS-595 to a subject every two weeks. In another embodiment, the dosing method comprises administering a dose of SNS-595 to a subject every three weeks. In another embodiment, the dosing method comprises administering a dose of SNS-595 to a subject every four weeks.

[0018] In another embodiment, the dosing method comprises a cycle wherein the cycle comprises administering a dose of SNS-595 to a subject every week for three weeks followed by a period of at least two weeks where no SNS-595 is administered to said subject and wherein the cycle is repeated a plurality of times. In another embodiment, the period where no SNS-595 is administered is two weeks. In another embodiment, the period where no SNS-595 is administered is three weeks.

[0019] Thus, the invention provides SNS-595 for use in a method of treating a hematologic cancer such as leukemias and lymphomas is provided. The method comprises:

i) administering a dose of 60 mg/m²-150 mg/m² of SNS-595 to a patient;

i) waiting a period of at least two days where the subject is not administered any SNS-595;

iii) administering another dose of 60 mg/m²-150 mg/m² of SNS-595 to the patient; and,

iv) repeating steps ii)-iii) a plurality of times.

[0020] In one embodiment, the method comprises administering supportive care to patients being treated with SNS-595. The method comprises:

a) administering to a patient a dose of 60 mg/m²-150 mg/m² of SNS-595 and

b) administering a therapeutically effective amount of a supportive care agent.

[0021] The supportive care agent is any substance that prevents or manages an adverse effect from SNS-595 treatment and is administered according to the appropriate dosing regimen for that substance. For example, different supportive care agents for treating nausea have different dosing regimen. While some are administered prophylactically, others are co-administered with SNS-595 while still others are administered after the administration of SNS-595. Illustrative examples of supportive care agents their doses and dosing regimens are found in The Physician's Desk Reference.

[0022] In one embodiment, the supportive care agent is an antiemetic. Illustrative examples of antiemetics include but are not limited to phenothiazines, butyrophenones, benzodiazapines, corticosteroids, serotonin antagonists, cannabinoids, and NK₁ receptor antagonists. Examples of phenothiazine antiemetics include prochlorperazine and trimethobenzamide. An example of a butyrophenone antiemetic is haloperidol. An example of a benzodiazapine antiemetic is lorazepam. An example of a corticosteroid antiemetic is dexamethasone. Examples of a serotonin antagonist antiemetic include ondansetron, granisetron, and dolasetron. An example of a cannabinoid antiemetic is dronabinol. An example of an NKI receptor antagonist is aprepitant.

[0023] In another embodiment, the antiemetic is prochlorperazine. In another embodiment, the antiemetic is prochlorperazine and the therapeutically effective amount is 10 mg. In another embodiment, the antiemetic is prochlorperazine and the therapeutically effective amount is an oral dose of 10 mg before the administration of SNS-595. In another embodiment, the antiemetic is prochlorperazine and the therapeutically effective amount is an oral dose of 10 mg every four to six hours as needed after the administration of SNS-595.

[0024] In another embodiment, the antiemetic is dexamethasone. In another embodiment, the antiemetic is dexamethasone and the therapeutically effective amount is at least 4 mg. In another embodiment, the antiemetic is dexamethasone and the therapeutically effective amount is an oral dose of 4 mg before the administration of SNS-595. In another embodiment, the antiemetic is dexamethasone and the therapeutically effective amount is an oral dose of 8 mg before the administration of SNS-595. In another embodiment, the antiemetic is dexamethasone and the therapeutically effective amount is an intravenous dose of between about 10 mg and about 20 mg before the administration of SNS-595. In another embodiment, the antiemetic is dexamethasone and the therapeutically effective amount is an oral dose of 4 mg every six to twelve hours as needed after the administration of SNS-595.

[0025] In another embodiment, the antiemetic is lorazepam. In another embodiment, the antiemetic is lorazepam and the therapeutically effective amount is 1 mg. In another embodiment, the antiemetic is lorazepam and the therapeutically effective amount is an oral dose of 1 mg before the administration of SNS-595. In another embodiment, the antiemetic is lorazepam and the therapeutically effective amount is an intravenous dose of 1 mg before the administration of SNS-595. In another embodiment, the antiemetic is lorazepam and the therapeutically effective amount is an oral dose of 1 mg every four to six hours as needed after the administration of SNS-595.

[0026] In another embodiment, the antiemetic is dolasetron. In another embodiment, the antiemetic is dolasetron and the therapeutically effective amount is 100 mg. In another embodiment, the antiemetic is dolasetron and the therapeutically effective amount is an oral dose of 100 mg before the administration of SNS-595. In another embodiment, the antiemetic is dolasetron and the therapeutically effective amount is an intravenous dose of 100 mg before the administration of SNS-595.

[0027] In another embodiment, the antiemetic is ondansetron. In another embodiment, the antiemetic is ondansetron and the therapeutically effective amount is at least 10 mg. In another embodiment, the antiemetic is ondansetron and the therapeutically effective amount is an intravenous dose of 10 mg before the administration of SNS-595. In another embodiment, the antiemetic is ondansetron and the therapeutically effective amount is an intravenous dose of 32 mg before the administration of SNS-595.

[0028] In another embodiment, the antiemetic is granisetron. In another embodiment, the antiemetic is granisetron and the therapeutically effective amount is 10 µg/kg. In another embodiment, the antiemetic is granisetron and the therapeutically effective amount is an intravenous dose of 10 µg/kg before the administration of SNS-595. In another embodiment, the antiemetic is granisetron and the therapeutically effective amount is at least 1 mg. In another embodiment, the antiemetic is granisetron and the therapeutically effective amount is an oral dose of 1 mg before the administration of SNS-595. In another embodiment, the antiemetic is granisetron and the therapeutically effective amount is an oral dose of 2 mg before the administration of SNS-595.

[0029] In another embodiment, the antiemetic is aprepitant. In another embodiment, the antiemetic is aprepitant and the therapeutically effective amount is at least 80 mg. In another embodiment, the antiemetic is aprepitant and the therapeutically effective amount is an oral dose of 125 mg before the administration of SNS-595. In another embodiment, the antiemetic is aprepitant and the therapeutically effective amount is a daily oral dose of 80 mg for at least two days after the administration of SNS-595.

[0030] In another embodiment, the supportive care agent is a hematopoietic agent. A hematopoietic agent is a molecule that stimulates hematopoiesis. Illustrative examples of hematopoietic agents include but are not limited to granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony stimulating factor (GM-CSF), erythropoietin and erythropoiesis stimulating protein, and derivatives thereof. Examples of G-CSF include but are not limited to filgrastim and its derivatives including pegfilgrastim. An example of GM-CSF includes sargramostim. An example of erythropoietin is epoetin alfa. An example of erythropoiesis stimulating protein is darbepoetin alfa.

[0031] In another embodiment, the hematopoietic agent is G-CSF. In another embodiment, the hematopoietic agent is filgrastim. In another embodiment, the hematopoietic agent is filgrastim and the therapeutically effective amount is at least 4 µg/kg. In another embodiment, the hematopoietic agent is filgrastim and the therapeutically effective amount is a daily dose of at least 4 µg/kg for at least 7 days after the administration of SNS-595. In another embodiment, the hematopoietic agent is filgrastim and the therapeutically effective amount is a daily subcutaneous dose of between about 4 µg/kg and about 8 µg/kg for at least 7 days starting from the third day after the administration of SNS-595. In another embodiment, the hematopoietic agent is filgrastim and the therapeutically effective amount is a daily subcutaneous dose of between about 4 µg/kg and about 10 µg/kg for at least 14 days starting from the third day after the administration of SNS-595.

[0032] In another embodiment, the hematopoietic agent is pegfilgrastim. In another embodiment, the hematopoietic agent is pegfilgrastim and the therapeutically effective amount is 6 mg. In another embodiment, the hematopoietic agent is pegfilgrastim and the therapeutically effective amount is a daily subcutaneous dose of 6 mg after the administration of SNS-595. In another embodiment, the hematopoietic agent is pegfilgrastim and the therapeutically effective amount is 100 µg/kg. In another embodiment, the hematopoietic agent is pegfilgrastim and the therapeutically effective amount is a daily dose of 100 µg/kg after the administration of SNS- 595.

[0033] In another embodiment, the hematopoietic agent is GM-CSF. In another embodiment, the hematopoietic agent is sargramostim. In another embodiment, the hematopoietic agent is sargramostim and the therapeutically effective amount is 250 µg/m². In another embodiment, the hematopoietic agent is sargramostim and the therapeutically effective amount is a daily intravenous or subcutaneous dose of 250 µg/m². In another embodiment, the hematopoietic agent is sargramostim and the therapeutically effective amount is a daily intravenous or subcutaneous dose of 250 µg/m² as needed starting from the third day after the administration of SNS-595. In another embodiment, the hematopoietic agent is sargramostim and the therapeutically effective amount is a daily intravenous or subcutaneous dose of 250 µg/m² as needed starting from the tenth day after the administration of SNS-595.

[0034] In another embodiment, the hematopoietic agent is erythropoietin. In another embodiment, the hematopoietic agent is epoetin alfa. In another embodiment, the hematopoietic agent is epoetin alfa and the therapeutically effective amount is at least 150 units/kg. In another embodiment, the hematopoietic agent is epoetin alfa and the therapeutically effective amount is an intravenous or subcutaneous dose of 150 units/kg three times a week after the administration of SNS-595. In another embodiment, the hematopoietic agent is epoetin alfa and the therapeutically effective amount is an intravenous or subcutaneous dose of 300 units/kg three times a week after the administration of SNS-595. In another embodiment, the hematopoietic agent is epoetin alfa and the therapeutically effective amount is 40,000 units. In another embodiment, the hematopoietic agent is epoetin alfa and the therapeutically effective amount is a weekly dose of 40,000 units after the administration of SNS-595.

[0035] In another embodiment, the hematopoietic agent is erytliropoiesis stimulating protein. In another embodiment, the hematopoietic agent is darbepoetin alfa. In another embodiment, the hematopoietic agent is darbepoetin alfa and the therapeutically effective amount is between about 1.5 µg/kg and about 4.5 µg/kg. In another embodiment, the hematopoietic agent is darbepoetin alfa and the therapeutically effective amount is a weekly dose of between about 1.5 µg/kg and about 4.5 µg/kg.

[0036] The application also encloses the following items:

1. A method for treating cancer comprising administering to a patient a dose of 10 mg/m²-150 mg/m² of SNS-595.

2. The method of item 1 wherein the dose is 10 mg/m²- 100 mg/m².

3. The method of item 1 wherein the dose is 30 mg/m²-75 mg/m².

4. The method of item 1 wherein the dose is 40 mg/m²-80 mg/m².

5. The method of item 1 wherein the dose is 50 mg/m²-90 mg/m².

6. The method of item 1 wherein the cancer being treated is selected from the group consisting of bladder cancer, breast cancer, cervical cancer, colon cancer, esophageal cancer, head and neck cancer, leukemia, liver cancer, lung cancer, lymphoma, melanoma, myeloma, neuroblastoma, ovarian cancer, pancreatic cancer, prostate cancer, renal cancer, sarcoma, skin cancer, stomach cancer, testicular cancer, thyroid cancer, and uterine cancer.

7. The method of item 1 wherein the dose is 10 mg/m²- 100 mg/m² and the cancer being treated is a solid tumor.

8. The method of item 1 wherein the dose is 60 mg/m²-150 mg/m² and the cancer being treated is a hematologic cancer.

9. The method of item 1 additionally comprising administering to a patient a therapeutically effective dose of a supportive care agent.

10. The method of item 9 wherein the supportive care agent is an antiemetic.

11. The method of item 9 wherein the supportive care agent is a hematopoietic agent.

12. A method comprising: i) administering a dose of 10 mg/m²-150 mg/m² of SNS-595 to a patient; ii) waiting a period of at least one day where the subject is not administered any SNS-595; iii) administering another dose of 10 mg/m² -150 mg/m² of SNS-595 to the patient; and, iv) repeating steps ii)-iii) a plurality of times.

13. The method of item 12 wherein the waiting period is at least 2 days.

14. The method of item 12 wherein the waiting period is at least 3 days.

15. The method of item 12 wherein the waiting period is at least 6 days.

16. The method of item 12 wherein the waiting period is at least 13 days.

17. The method of item 10 wherein the waiting period is at least 20 days.

18. The method of item 10 wherein the waiting period is at least 27 days

19. A method comprising administering a weekly dose of SNS-595 to a patient.

20. A method comprising administering a dose of SNS-595 to a patient every three weeks.

21. A method comprising a cycle wherein the cycle comprises administering a dose of SNS-595 to a subject every week for three weeks followed by a period of at least two weeks where no SNS-595 is administered to said subject and wherein the cycle is repeated a plurality of times.

22. A method for treating cancer comprising administering to a patient a dose of 10 mg/m²-150 mg/m² of SNS-595 and administering a patient a therapeutically effective dose of a hematopoietic agent.

23. The method of item 22 wherein the hematopoietic agent is G-CSF.

24. The method of item 22 wherein the hematopoietic agent is GM-CSF.

25. The method of item 22 wherein the hematopoietic agent is erythropoietin.

26. The method of item 22 wherein the hematopoietic agent is erythropoiesis stimulating protein.

EXAMPLE 1

Pharmaceutical composition suitable for injection or intravenous infusion

[0037] Acidic compositions (< pH 4) provided the appropriate balance of increased solubility of SNS-595 and desirable pharmaceutical properties (e.g. increased patient comfort by causing less irritation at the delivery site). An illustrative example of a suitable composition comprises: 10 mg SNS-595 per mL of aqueous solution of 4.5% sorbitol that is adjusted to pH 2.5 with methanesulfonic acid. One protocol for making such a solution includes the following for making a 100mg/10mL presentation: 100 mg of SNS-595 and 450 mg D-sorbitol are added to distilled water; the volume is brought up to a volume of 10 mL; and the pH of the resulting solution is adjusted to 2.5 with methanesulfonic acid. The resulting composition is also suitable for lyophilization. The lyophilized form is then reconstituted with sterile water to the appropriate concentration prior to use.

EXAMPLE 2

Pharmacokinetics of SNS-595 in cancer patients

[0038] SNS-595 was administered to enrolled patients for up to six cycles. A cycle is defined as a three-week period, with SNS-595 administered on the first day of each cycle (day 0), followed by at least 21 days of observation. SNS-595 was administered to cohorts of at least 3 patients and dose escalation occurred by sequential cohort. Doses of SNS-595 were linear with AUC°° and its pharmacokinetic properties were remarkably consistent among patients in the same cohort. Figure 1 depicts the plasma concentrations of SNS-595 over time among the various patient cohorts and Table 1 shows the pharmacokinetic parameters derived there from.

Table 1

Dose (mg/m2)	HL (hr)	CO (ng/mL)	Cmax (ng/mL)	AUClast (hr*ng/mL)	AUCINF_obs (hr*ng/mL)	CL_obs (mL/min/kg)	Vz_obs (L/kg)	Vss_obs (L/kg)	MRTINF_obs (hr)
3	16.27	152.25	138,80	750.08	1139.55	1.14	1.55	1.44	21.96
SD	4.871	82.282	80.566	87.622	263	0.318	0.297	0.277	6.836
6	20.69	379.69	347.00	2400.00	2990.29	0.71	1.28	1.24	29.05
SD	0.327	243.598	214.96	170.556	245.64	0.153	0.295	0.218	1.15
12	17.81	2888.66	2246.67	5395.53	6329.15	0.76	1.17	1.07	23.67
SD	3.896	1302.71	1065.145	292.281	181.804	0.126	0.258	0.184	5.021
24	16.14	2924.46	2703.33	11133.03	12655.32	0.83	1.15	1.06	21.65
SD	2.601	2884.702	2573.02	468.453	851.458	0.108	0.124	0.165	5.261
48	21.32	1984.52	2868.00	21098.53	27347.36	0.99	1.57	1.46	28.90
SD	6.32	189.677	2379.899	9405.346	14382.787	0.616	0.567	0.47	8.91
60	17.63	4797.47	4537.50	28112.17	33616.18	0.83	1.20	1.08	23.71
SD	4.15	2215.20	1947.89	9127.12	13081.44	0.352	0.37	0.218	6.93

Claims

1. A compound which is (+)-1,4-dihydro-7-[(3S,4S)-3-methoxy-4-(methylamino)-1-pymolidinyl]-4-oxo-1-(2-thiazolyl)-1,8-naphthyridine-3-carboxylic acid for use in a method of treating leukemia in a patient, wherein the method comprises administering the compound to the patient in a dose of 60 mg/m² - 150 mg/m².

2. The compound for use of claim 1, wherein the method comprises administering the compound to the patient in combination with a supportive care agent selected from the group consisting of phenothiazine, butyrophenone, benzodiazapine, corticosteroid, serotonin antagonist, cannabinoid, NK receptor antagonist, granulocyte colony stimulating factor, granulocyte macrophage colony stimulating factor, erythropoietin, erythropoiesis stimulating protein, darbepoietin alfa, and derivatives thereof.

3. The compound for use of claim 1, wherein the method comprises:

i) administering a dose of the compound of 60 mg/m²- 150 mg/m²;

ii) waiting a period of at least two days where the compound is not administered;

iii) administering another dose of the compound of 60 mg/m² - 150 mg/m²; and

iv) repeating steps ii)-iii) a plurality of times.

4. The compound for use of claim 3, wherein the waiting period is at least 2 days, at least 3 days, at least 6 days, at least 13 days, at least 20 days, or at least 27 days.

5. The compound for use of claim 3, wherein the waiting period is at least 6 days and steps ii) through iii) are repeated at least three, or at least five times.

6. The compound for use of claim 3, wherein the waiting period is at least 20 days and steps ii) through iii) are repeated at least three, or at least five times.

7. The compound for use of claim 3, wherein the waiting period is at least 27 days and steps ii) through iii) are repeated at least three, or at least five times.

8. The compound for use of claim 1, wherein the method comprises administering the dose of 60 mg/m² - 150 mg/m² every one, two, three, or four weeks.

9. The compound for use of claim 1, wherein the method comprises a cycle, wherein the cycle comprises administering the dose of 60 mg/m² - 150 mg/m² every week for three weeks followed by a period of at least two weeks where the compound is not administered, and wherein the cycle is repeated a plurality of times.

10. The compound for use of claim 1, wherein the weekly dose is 60 mg/m² - 90 mg/m², 60 mg/m² - 70 mg/m², 65 mg/m² - 75 mg/m² 70 mg/m² - 80 mg/m², 75 mg/m² - 85 mg/m², 80 mg/m² - 90 mg/m², or 85 mg/m² - 95 mg/m², 90 mg/m² - 100 mg/m², 95 mg/m² - 105 mg/m², 100 mg/m² - 110 mg/m², 105 mg/m² - 115 mg/m², 110 mg/m²-120 mg/m², 115 mg/m² - 125 mg/m², 120 mg/m² 130 mg/m², 125 mg/m²- 135 mg/m², 130 mg/m² - 140 mg/m², 135 mg/m² - 145 mg/m², 140 mg/m² - 150 mg/m².

11. The compound for use of claim 1, wherein the method comprises administering the compound in form of a pharmaceutical composition comprising the compound and an acid in an aqueous solution, wherein the pH of the solution is 2-3.5.

12. The compound for use of claim 11, wherein the acid is acetic acid, ascorbic acid, benzene-sulfonic acid, ethanesulfonic acid, glycolic acid, hydroxyethanesulfonic acid, lactic acid, maleic acid, methanesulfonic acid, propionic acid, succinic acid, sulfuric acid, trifluoroacetic acid, or toluenesulfonic acid.

13. The compound for use of claim 11, wherein the acid is methanesulfonic acid.

Ansprüche

1. Verbindung, die (+)-1,4-Dihydro-7-[(3S,4S)-3-methoxy-4-(methylamino)-1-pymolidinyl]-4-oxo-1-(2-thiazolyl)-1,8-naphthyridin-3-carbonsäure ist, für die Verwendung in einem Verfahren zur Behandlung von Leukämie bei einem Patienten, wobei das Verfahren das Verabreichen der Verbindung an den Patienten in einer Dosis von 60 mg/m² - 150 mg/m² umfasst.

2. Verbindung für die Verwendung nach Anspruch 1, wobei das Verfahren das Verabreichen der Verbindung an den Patienten in Kombination mit einem unterstützenden Behandlungsmittel ausgewählt aus der Gruppe bestehend aus Phenothiazin, Butyrophenon, Benzodiazapin, Kortikosteroid, Serotonin Antagonist, Cannabinoid, NK Rezeptor-Antagonist, Granulozyt Koloniestimulierender Faktor, Granulozyt-Macrophage Koloniestimulierender Faktor, Erythropoietin, Erythropoesestimulierendes Protein, Darbepoietin alfa und Derivative davon umfasst.

3. Verbindung für die Verwendung nach Anspruch 1, wobei das Verfahren umfasst:

i) Verabreichen einer Dosis der Verbindung von 60 mg/m² - 150 mg/m²;

ii) Warten über einen Zeitraum von mindestens einem Tag, in dem die Verbindung nicht verabreicht wird;

iii) Verabreichen einer weiteren Dosis der Verbindung von 60 mg/m² - 150 mg/m²; und

iv) mehrmaliges Wiederholen der Schritte ii)-iii).

4. Verbindung für die Verwendung nach Anspruch 3, wobei der Wartezeitraum mindestens 2 Tage, mindestens 3 Tage, mindestens 6 Tage, mindestens 13 Tage, mindestens 20 Tage oder mindestens 27 Tage ist.

5. Verbindung für die Verwendung nach Anspruch 3, wobei der Wartezeitraum mindestens 6 Tage ist und die Schritte ii) bis iii) mindestens 3-mal oder mindestens 5-mal wiederholt werden.

6. Verbindung für die Verwendung nach Anspruch 3, wobei der Wartezeitraum mindestens 20 Tage ist und die Schritte ii) bis iii) mindestens 3-mal oder mindestens 5-mal wiederholt werden.

7. Verbindung für die Verwendung nach Anspruch 3, wobei der Wartezeitraum mindestens 27 Tage ist und die Schritte ii) bis iii) mindestens 3-mal oder mindestens 5-mal wiederholt werden.

8. Verbindung für die Verwendung nach Anspruch 1, wobei das Verfahren das Verabreichen der Dosis von 60 mg/m² - 150 mg/m² jede, alle zwei, drei oder vier Wochen umfasst.

9. Verbindung für die Verwendung nach Anspruch 1, wobei das Verfahren einen Zyklus umfasst, wobei der Zyklus das Verabreichen der Dosis von 60 mg/m² - 150 mg/m² jede Woche für drei Wochen gefolgt von einem Zeitraum von mindestens zwei Wochen, in dem die Verbindung nicht verabreicht wird, umfasst und wobei der Zyklus mehrmals wiederholt wird.

10. Verbindung für die Verwendung nach Anspruch 1, wobei die wöchentliche Dosis 60 mg/m² - 90 mg/m², 60 mg/m² - 70 mg/m², 65 mg/m² - 75 mg/m², 70 mg/m² - 80 mg/m², 75 mg/m² - 85 mg/m², 80 mg/m² - 90 mg/m², 85 mg/m² - 95 mg/m², 90 mg/m² - 100 mg/m², 95 mg/m² - 105 mg/m², 100 mg/m² - 110 mg/m², 105 mg/m² - 115 mg/m², 110 mg/m² - 120 mg/m², 115 mg/m² - 125 mg/m², 120 mg/m² - 130 mg/m², 125 mg/m² - 135 mg/m², 130 mg/m² - 140 mg/m², 135 mg/m² - 145 mg/m², 140 mg/m² - 150 mg/m² ist.

11. Verbindung für die Verwendung nach Anspruch 1, wobei das Verfahren das Verabreichen der Verbindung in Form einer pharmazeutischen Zusammensetzung umfassend die Verbindung und eine Säure in einer wässrigen Lösung umfasst, wobei der pH der Lösung 2-3.5 ist.

12. Verbindung für die Verwendung nach Anspruch 11, wobei die Säure Essigsäure, Ascorbinsäure, Benzolsulfonsäure, Ethansulfonsäure, Glycolsäure, Hydroxyethansulfonsäure, Milchsäure, Maleinsäure, Methansulfonsäure, Propionsäure, Succinsäure, Schwefelsäure, Trifluoressigsäure oder Toluolsulfonsäure ist.

13. Verbindung für die Verwendung nach Anspruch 11, wobei die Säure Methansulfonsäure ist.

Revendications

1. Composé qui est l'acide (+)-1,4-dihydro-7-[(3S,4S)-3-méthoxy-4-(méthylamino)-1-pyrrolidinyl]-4-oxo-1-(2-thiazolyl)-1,8-naphtyridine-3-carboxylique pour une utilisation dans un procédé pour le traitement de la leucémie chez un patient, le procédé comprenant l'administration du composé au patient à une dose de 60 mg/m²-150 mg/m².

2. Composé pour une utilisation selon la revendication 1, le procédé comprenant l'administration du composé au patient en combinaison avec un agent de traitement de soutien choisi dans le groupe constitué par la phénothiazine, la butyrophénone, la benzodiazapine, un corticostéroïde, un antagoniste de la sérotonine, un cannabinoïde, un antagoniste d'un récepteur NK, un facteur de stimulation des colonies de granulocytes, un facteur de stimulation des colonies de granulocytes macrophages, l'érythropoïétine, une protéine de stimulation de l'érythropoïèse, la darbépoétine alpha et des dérivés correspondants.

3. Composé pour l'utilisation selon la revendication 1, le procédé comprenant :

i) l'administration d'une dose du composé de 60 mg/m²-150 mg/m² ;

ii) l'attente pendant un laps de temps d'au moins un jour pendant lequel le composé n'est pas administré ;

iii) l'administration d'une autre dose du composé de 60 mg/m²-150 mg/m² ; et

iv) la répétition des étapes ii)-iii) une pluralité de fois.

4. Composé pour l'utilisation selon la revendication 3, le laps de temps d'attente étant d'au moins 2 jours, d'au moins 3 jours, d'au moins 6 jours, d'au moins 13 jours, d'au moins 20 jours ou d'au moins 27 jours.

5. Composé pour l'utilisation selon la revendication 3, le laps de temps d'attente étant d'au moins 6 jours et les étapes ii) à iii) étant répétées au moins trois ou au moins cinq fois.

6. Composé pour l'utilisation selon la revendication 3, le laps de temps d'attente étant d'au moins 20 jours et les étapes ii) à iii) étant répétées au moins trois ou au moins cinq fois.

7. Composé pour l'utilisation selon la revendication 3, le laps de temps d'attente étant d'au moins 27 jours et les étapes ii) à iii) étant répétées au moins trois ou au moins cinq fois.

8. Composé pour l'utilisation selon la revendication 1, le procédé comprenant l'administration de la dose de 60 mg/m²-150 mg/m² toutes les une, deux, trois ou quatre semaines.

9. Composé pour l'utilisation selon la revendication 1, le procédé comprenant un cycle, le cycle comprenant l'administration de la dose de 60 mg/m²-150 mg/m² chaque semaine pendant trois semaines, suivies d'une période d'au moins deux semaines pendant lequel le composé n'est pas administré et le cycle étant répété une pluralité de fois.

10. Composé pour l'utilisation selon la revendication 1, la dose hebdomadaire étant de 60 mg/m²-90 mg/m², 60 mg/m²-70 mg/m², 65 mg/m²-75 mg/m², 70 mg/m²-80 mg/m², 75 mg/m²-85 mg/m², 80 mg/m²-90 mg/m², 85 mg/m²-95 mg/m², 90 mg/m²-100 mg/m², 95 mg/m²-105 mg/m², 100 mg/m²-110 mg/m², 105 mg/m²-115 mg/m², 110 mg/m²-120 mg/m², 115 mg/m²-125 mg/m², 120 mg/m²-130 mg/m², 125 mg/m²-135 mg/m², 130 mg/m²-140 mg/m², 135 mg/m²-145 mg/m², 140 mg/m²-150 mg/m².

11. Composé pour l'utilisation selon la revendication 1, le procédé comprenant l'administration du composé sous forme d'une composition pharmaceutique, comprenant le composé et un acide en solution aqueuse, le pH de la solution étant de 2-3,5.

12. Composé pour l'utilisation selon la revendication 11, l'acide étant l'acide acétique, l'acide ascorbique, l'acide benzènesulfonique, l'acide éthanesulfonique, l'acide glycolique, l'acide hydroxyéthanesulfonique, l'acide lactique, l'acide maléique, l'acide méthanesulfonique, l'acide propionique, l'acide succinique, l'acide sulfurique, l'acide trifluoroacétique ou l'acide toluènesulfonique.

13. Composé pour l'utilisation selon la revendication 11, l'acide étant l'acide méthanesulfonique.