(19)

(11)

EP 2 300 613 A2

(12)

(88) Date of publication A3: 06.05.2010 (43) Date of publication: 30.03.2011 Bulletin 2011/13 (21) Application number: 09767773.6 (22) Date of filing: 18.06.2009

(51) International Patent Classification (IPC):  C12N 15/54(2006.01) C12N 9/10(2006.01) (86) International application number: PCT/US2009/047862 (87) International publication number: WO 2009/155464 (23.12.2009 Gazette 2009/52)

(84) Designated Contracting States: AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK TR Designated Extension States: AL BA RS (30) Priority: 18.06.2008 US 73754 P (71) Applicant: Life Technologies Corporation Carlsbad, CA 92008 (US)	(72) Inventor: XI, Lei Foster City, CA 94404 (US) (74) Representative: Wöhler, Christian  Life Technologies Frankfurter Straße 129 B 64293 Darmstadt 64293 Darmstadt (DE)