SECRETION EXPRESSION OF ANTIBIOTIC PEPTIDE CAD IN BACILLUS SUBTILIS AND EXPRESSION SYSTEM OF RECOMBINATION BACILLUS SUBTILIS

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EP 2 319 927 B8

(12)	CORRECTED EUROPEAN PATENT SPECIFICATION
	Note: Bibliography reflects the latest situation

(15)	Correction information:
	Corrected version no 1 (W1 B1)

(48)	Corrigendum issued on:
	30.11.2016 Bulletin 2016/48

(45)	Mention of the grant of the patent:
	07.09.2016 Bulletin 2016/36

(21)	Application number: 09799930.4

(22)	Date of filing: 21.07.2009

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International Patent Classification (IPC):

C12N 15/12^(2006.01)
C12N 15/75^(2006.01)
C12N 1/21^(2006.01)

C12N 15/62^(2006.01)
C07K 14/435^(2006.01)
C12R 1/125^(2006.01)

(86)	International application number:
	PCT/CN2009/000815

(87)	International publication number:
	WO 2010/009614 (28.01.2010 Gazette 2010/04)

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SECRETION EXPRESSION OF ANTIBIOTIC PEPTIDE CAD IN BACILLUS SUBTILIS AND EXPRESSION SYSTEM OF RECOMBINATION BACILLUS SUBTILIS

SEKRETIONSEXPRESSION EINES ANTIBIOTISCHEN CAD-PEPTIDS IN BACILLUS SUBTILIS UND EXPRESSIONSSYSTEM FÜR REKOMBINANTEN BACILLUS SUBTILIS

EXPRESSION SÉCRÉTOIRE DE PEPTIDE ANTIBIOTIQUE CAD DANS BACILLUS SUBTILIS ET SYSTÈME D EXPRESSION DE BACILLUS SUBTILIS RECOMBINANT

(84)	Designated Contracting States:
	AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR

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Priority:

22.07.2008 CN 200810132258

(43)	Date of publication of application:
	11.05.2011 Bulletin 2011/19

(73)	Proprietor: Linzhou Sinagri Yingtai Biological Peptides Co., Ltd.
	Linzhou City Henan 456550 (CN)

(72)	Inventors:
	QIAO, Shiyan Beijing 100193 (CN) ZHU, Faming Beijing 100193 (CN) GUO, Wenjiang Beijing 100085 (CN)

(74)	Representative: Vidon Brevets & Stratégie
	16B, rue de Jouanet B.P. 90333 Technopole Atalante 35703 Rennes Cedex 7 35703 Rennes Cedex 7 (FR)

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References cited: :

WO-A2-03/087142
WO-A2-2008/140582
CN-A- 101 307 316
US-A1- 2006 134 630

WO-A2-2005/003313
CN-A- 101 182 351
US-A1- 2002 025 537
US-A1- 2006 134 630

MOON ET AL: "Recombinant expression, isotope labeling, refolding, and purification of an antimicrobial peptide, piscidin", PROTEIN EXPRESSION AND PURIFICATION, ACADEMIC PRESS, SAN DIEGO, CA, vol. 51, no. 2, 30 November 2006 (2006-11-30), pages 141-146, XP005727975, ISSN: 1046-5928, DOI: 10.1016/J.PEP.2006.07.010
XU ET AL: "High-level expression of the recombinant hybrid peptide cecropinA(1-8)-magainin2(1-12) with an ubiquitin fusion partner in Escherichia coli", PROTEIN EXPRESSION AND PURIFICATION, ACADEMIC PRESS, SAN DIEGO, CA, vol. 55, no. 1, 18 August 2007 (2007-08-18), pages 175-182, XP022206989, ISSN: 1046-5928, DOI: 10.1016/J.PEP.2007.04.018
WU X-C ET AL: "ENGINEERING A BACILLUS SUBTILIS EXPRESSION-SECRETION SYSTEM WITH A STRAIN DEFICIENT IN SIX EXTRACELLULAR PROTEASES", JOURNAL OF BACTERIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, DC; US, vol. 173, no. 16, 1 August 1991 (1991-08-01), pages 4952-4958, XP009005082, ISSN: 0021-9193
HUANG YA-DONG ET AL: "Modification of cecropin AD gene and its expression in Pichia pastoris", HUANAN LIGONG DAXUE XUEBAO - JOURNAL OF SOUTH CHINA UNIVERSITY OF TECHNOLOGY, GUANGZHOU, CH, vol. 30, no. 3, 28 February 2002 (2002-02-28), pages 13-16, XP008142707, ISSN: 1000-565X
BUTT T R ET AL: "SUMO fusion technology for difficult-to-express proteins", PROTEIN EXPRESSION AND PURIFICATION, ACADEMIC PRESS, SAN DIEGO, CA, vol. 43, no. 1, 1 September 2005 (2005-09-01), pages 1-9, XP027390259, ISSN: 1046-5928, DOI: 10.1016/J.PEP.2005.03.016 [retrieved on 2005-08-08]
X. CHEN ET AL: "Novel Expression Vector for Secretion of Cecropin AD in Bacillus subtilis with Enhanced Antimicrobial Activity", ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, vol. 53, no. 9, 1 September 2009 (2009-09-01), pages 3683-3689, XP55007631, ISSN: 0066-4804, DOI: 10.1128/AAC.00251-09
HUANG YA-DONG ET AL ET AL.: 'Modification of cecropin AD gene and its expression in Pichia pastoris' JOURNAL OF SOUTH CHINA UNIVERSITY OF TECHNOLOGY(NATURAL SCIENCE EDITION) vol. 30, no. 2, 28 February 2002, XP008142707
YANG MING-MING ET AL: "Construction and characterization of a novel maltose inducible expression vector in Bacillus subtilis", BIOTECHNOLOGY LETTERS, SPRINGER NETHERLANDS, DORDRECHT, vol. 28, no. 21, 3 August 2006 (2006-08-03), pages 1713-1718, XP019433285, ISSN: 1573-6776, DOI: 10.1007/S10529-006-9125-4
G. JI ET AL: "Reduction of arsenate to arsenite by the ArsC protein of the arsenic resistance operon of Staphylococcus aureus plasmid pI258.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 89, no. 20, 15 October 1992 (1992-10-15), pages 9474-9478, XP055073660, ISSN: 0027-8424, DOI: 10.1073/pnas.89.20.9474

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