(19)
(11) EP 2 339 016 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
27.01.2016 Bulletin 2016/04

(45) Mention of the grant of the patent:
25.11.2015 Bulletin 2015/48

(21) Application number: 11150877.6

(22) Date of filing: 28.03.2003
(51) International Patent Classification (IPC): 
C12P 21/06(2006.01)
C12N 1/20(2006.01)
C12N 15/74(2006.01)
 C12N 9/54(2006.01)
C12N 15/00(2006.01)
C12N 1/00(2006.01)

(54)

Enhanced production of subtilisins in Bacillus

Erhöhte Expression von Subtilisinen in Bacillus

Expression de subtilisins augmentée dans Bacillus

(84) Designated Contracting States:
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR

(30) Priority: 29.03.2002 US 368949 P
29.03.2002 US 368858 P
29.04.2002 US 376343 P

(43) Date of publication of application:
29.06.2011 Bulletin 2011/26

(60) Divisional application:
15196066.3

(62) Application number of the earlier application in accordance with Art. 76 EPC:
03726142.7 / 1495128

(73) Proprietor: Danisco US Inc.
Palo Alto, California 94304 (US)

(72) Inventors:
  • Ferrari, Eugenio
    San Bruno, CA 94066 (US)
  • Harbison, Carole
    Ithaca, NY 14850 (US)
  • Rashid, Harunur
    Sunnyvale, CA 94087 (US)
  • Weyler, Walter
    San Francisco, CA 94112 (US)

(74) Representative: van Kooij, Adriaan 
Arnold & Siedsma Bezuidenhoutseweg 57
2594 AC The Hague
2594 AC The Hague (NL)


(56) References cited: : 
EP-A1- 0 414 297
 WO-A1-01/96577
   
  • DEBARBOUILLE MICHEL ET AL: "Role of bkdR, a transcriptional activator of the SigL-dependent isoleucine and valine degradation pathway in Bacillus subtilis", JOURNAL OF BACTERIOLOGY, vol. 181, no. 7, April 1999 (1999-04), pages 2059-2066, XP002385304, ISSN: 0021-9193
  • BELITSKY BORIS E ET AL: "Role and regulation of Bacillus subtilis glutamate dehydrogenase genes", JOURNAL OF BACTERIOLOGY, vol. 180, no. 23, December 1998 (1998-12), pages 6298-6305, XP002385305, ISSN: 0021-9193
  • GARDAN R ET AL: "Expression of the rocDEF operon involved in arginine catabolism in Bacillus subtilis.", JOURNAL OF MOLECULAR BIOLOGY. 23 JUN 1995, vol. 249, no. 5, 23 June 1995 (1995-06-23) , pages 843-856, XP002385306, ISSN: 0022-2836
  • KUNST F ET AL: "THE COMPLETE GENOME SEQUENCE OF THE GRAM-POSITIVE BACTERIUM BACILLUS SUBTILIS", NATURE, NATURE PUBLISHING GROUP, LONDON, GB, vol. 390, 1997, pages 249-256, XP000919353, ISSN: 0028-0836
  • TSUGE KENJI ET AL: "The Genes degQ, pps, and lpa-8 (sfp) are responsible for conversion of Bacillus subtilis 168 to plipastatin production", ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, vol. 43, no. 9, September 1999 (1999-09), pages 2183-2192, XP002632934, ISSN: 0066-4804
  • COUTTE F ET AL: "Effect of pps disruption and constitutive expression of srfA on surfactin productivity, spreading and antagonistic properties of Bacillus subtilis 168 derivatives", JOURNAL OF APPLIED MICROBIOLOGY, vol. 109, no. 2, August 2010 (2010-08), pages 480-491, XP002632917, ISSN: 1364-5072
  • WU X-C ET AL: "ENGINEERING A BACILLUS SUBTILIS EXPRESSION-SECRETION SYSTEM WITH A STRAIN DEFICIENT IN SIX EXTRACELLULAR PROTEASES", JOURNAL OF BACTERIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, DC; US, vol. 173, no. 16, 1 August 1991 (1991-08-01), pages 4952-4958, XP009005082, ISSN: 0021-9193
  • WONG S-L: "ADVANCES IN THE USE OF BACILLUS SUBTILIS FOR THE EXPRESSION AND SECRETION OF HETEROLOGOUS PROTEINS", CURRENT OPINION IN BIOTECHNOLOGY, LONDON, GB, vol. 6, no. 5, 1 October 1995 (1995-10-01) , pages 517-522, XP000607468, ISSN: 0958-1669, DOI: 10.1016/0958-1669(95)80085-9
  • SARVAS M: "Gene expression in recombinant Bacillus", BIOPROCESS TECHNOLOGY, DEKKER, US, vol. 22, 1 January 1995 (1995-01-01), pages 53-120, XP009167177, ISSN: 0888-7470
   
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