(19)
(11) EP 2 390 258 A8

(12) CORRECTED EUROPEAN PATENT APPLICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 A1)

(48) Corrigendum issued on:
11.01.2012 Bulletin 2012/02

(43) Date of publication:
30.11.2011 Bulletin 2011/48

(21) Application number: 10178608.5

(22) Date of filing: 28.08.1998
(51) International Patent Classification (IPC): 
C07H 21/04(2006.01)
C12P 19/34(2006.01)
 C12N 9/12(2006.01)
(84) Designated Contracting States:
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
Designated Extension States:
AL LT LV MK RO SI

(30) Priority: 29.08.1997 US 56263 P
26.09.1997 US 60131 P
13.05.1998 US 85247 P
27.08.1998 US 141522 P

(62) Application number of the earlier application in accordance with Art. 76 EPC:
98943434.5 / 1012161

(71) Applicant: Life Technologies Corporation
Carlsbad, CA 92008 (US)

(72) Inventors:
  • Yang, Shuwei
    Rockville, MD 20855 (US)
  • Chatterjee, Deb, K.
    North Potomac, MD 20878 (US)

(74) Representative: Williams, Richard Andrew Norman 
Harrison Goddard Foote 40- 43 Chancery Lane
London WC2A 1JA
London WC2A 1JA (GB)

 
Remarks:
This application was filed on 23-09-2010 as a divisional application to the application mentioned under INID code 62.
Remarks:
Claims filed after the date of filing of the application (Rule 68(4) EPC).
 


(54) High fidelity polymerases and uses thereof


(57) The present invention relates to a DNA and RNA polymerases which have increased fidelity (or reduced misincorporation rate). In particular, the invention relates to a method of making such polymerases by modifying or mutating the nucleotide binding domain of the polymerase (e.g., the O-helix). The invention also relates to DNA molecules containing the genes encoding the polymerases of the invention, to host cells containing such DNA molecules and to methods to make the polymerases using the host cells. The polymerases are particularly suited for nucleic acid synthesis, sequencing, amplification and cDNA synthesis.