(19)
(11) EP 2 418 287 A3

(12) EUROPEAN PATENT APPLICATION

(88) Date of publication A3:
04.07.2012 Bulletin 2012/27

(43) Date of publication A2:
15.02.2012 Bulletin 2012/07

(21) Application number: 10185191.3

(22) Date of filing: 13.10.2003
(27) Previously filed application:
 13.10.2003 PCT/NL03/00690
(51) International Patent Classification (IPC): 
C12Q 1/68(2006.01)
(84) Designated Contracting States:
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR
Designated Extension States:
AL LT LV MK

(30) Priority: 11.10.2002 US 417779 P

(62) Application number of the earlier application in accordance with Art. 76 EPC:
03756746.8 / 1549764

(71) Applicant: Erasmus Universiteit Rotterdam
3015 GE Rotterdam (NL)

(72) Inventors:
  • van Dongen, Jacobus Johannes Maria
    2914 LE, Nieuwerkerk aan den IJssel (NL)
  • Langerak, Anthonie Willem
    2992 WJ, Barendrecht (NL)
  • Schuuring, Eduardus Maria Dominicus
    9746 BM, Groningen (NL)
  • San Miquel, Jesus Fernando
    37900, Salamanca (ES)
  • Garcia Sanz, Ramon
    37005, Salamanca (ES)
  • Parreira, Antonio
    1050-095, Lissabon (PT)
  • Smith, John Lewis
    SP5 2EL, Wilts (GB)
  • Lavender, Frances Louise
    Burley, Ringwood Hants, BH24 4EN (GB)
  • Morgan, Gareth John
    Leeds, LS8 1NZ (GB)
  • Evans, Paul Anthony Stuart
    North Yorkshire, WF 11 9JR (GB)
  • Kneba, Michael
    D-24259, Westensee (DE)
  • Hummel, Michael
    D-12105, Berlijn (DE)
  • Macintyre, Elizabeth Anne
    92190, Meudon (FR)
  • Bastard, Christian
    76680, Ardouval (FR)
  • Davi, Frederic Bernard Louis
    92190, Meudon (FR)
  • Brüggemann, Monika
    D-24118, Kiel (DE)

(74) Representative: Jansen, Cornelis Marinus et al
VEREENIGDE Johan de Wittlaan 7
2517 JR Den Haag
2517 JR Den Haag (NL)

   


(54) Nucleic acid amplification primers for PCR-based clonality studies of the TCR-beta gene


(57) The invention relates to PCR-based clonality studies for among others early diagnosis of lymphoproliferative disorders. Provided is a set of nucleic acid amplification primers comprising a forward primer, or a variant thereof, and a reverse primer, or a variant thereof, capable of amplifying a Vβ-Jβ TCRB rearrangement, and optionally a rearrangement selected from the group consisting of a VH-JH IGH rearrangement, a DH-JH IGH rearrangement, a VK-JK IGK rearrangement, a VK/intron-Kde IGK rearrangement, a Vλ-Jλ IGL rearrangement, a Dβ-Jβ TCRB rearrangement, a Vγ-Jγ TCRG rearrangement, a Vδ-Jδ TCRD rearrangement, a Dδ-Dδ TCRD rearrangement, a Dδ-Jδ TCRD rearrangement, a Vδ-Dδ TCRD rearrangement, or a translocation selected from t(11;14)(BCL1-IGH) and t(14;18)(BCL2-ICrH). The primers can be used in PCR-based clonality studies for early diagnosis of lymphoproliferative disorders and detection of minimal residual disease (MRD). Also provided is a kit comprising at least one set of primers of the invention.





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