(19)
(11) EP 2 423 302 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
30.09.2015 Bulletin 2015/40

(45) Mention of the grant of the patent:
08.07.2015 Bulletin 2015/28

(21) Application number: 09843569.6

(22) Date of filing: 30.09.2009
(51) International Patent Classification (IPC): 
C12N 5/071(2010.01)
C12Q 1/68(2006.01)
 C12N 5/10(2006.01)
(86) International application number:
PCT/CN2009/074358
(87) International publication number:
WO 2010/121465 (28.10.2010 Gazette 2010/43)

(54)

NEW SERUM-FREE MEDIUM FOR INDUCING PLURIPOTENT STEM CELLS QUICKLY WITH HIGH EFFICIENCY AND METHOD USING THEREOF

NEUES SERUMFREIES MEDIUM ZUR HOCHEFFIZIENTEN SCHNELLEN INDUZIERUNG PLURIPOTENTER STAMMZELLEN UND VERWENDUNGSVERFAHREN DAFÜR

NOUVEAU MILIEU SANS SÉRUM POUR L'INDUCTION RAPIDE DE CELLULES SOUCHES PLURIPOTENTES AVEC UN RENDEMENT ÉLEVÉ ET SON PROCÉDÉ D'UTILISATION

(84) Designated Contracting States:
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR

(30) Priority: 23.04.2009 CN 200910038883

(43) Date of publication of application:
29.02.2012 Bulletin 2012/09

(73) Proprietor: Guangzhou Institute Of Biomedicine And Health, Chinese Academy Of Sciences
Guangdong 510663 (CN)

(72) Inventors:
  • PEI, Duanqing
    Guangzhou Guangdong 510663 (CN)
  • CHEN, Jiekai
    Guangzhou Guangdong 510663 (CN)
  • LIU, Jing
    Guangzhou Guangdong 510663 (CN)

(74) Representative: Gulde & Partner 
Patent- und Rechtsanwaltskanzlei mbB Wallstraße 58/59
10179 Berlin
10179 Berlin (DE)


(56) References cited: : 
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CN-A- 101 126 077
US-A1- 2009 081 784
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CN-A- 1 673 356
CN-A- 1 800 368
CN-A- 1 844 374
CN-A- 1 974 764
CN-A- 101 092 606
CN-A- 101 333 513
   
  • AMIT M ET AL: "Feeder layer- and serum-free culture of human embryonic stem cells", BIOLOGY OF REPRODUCTION, NEW YORK, NY [U.A.] : ACADEM. PRESS, US, vol. 70, 1 January 2004 (2004-01-01), pages 837-845, XP002978624, ISSN: 0006-3363, DOI: 10.1095/BIOLREPROD.103.021147
  • TROND AASEN ET AL: "Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes", NATURE BIOTECHNOLOGY, vol. 26, no. 11, 1 November 2008 (2008-11-01), pages 1276-1284, XP055019438, ISSN: 1087-0156, DOI: 10.1038/nbt.1503
  • LUDWIG TENNEILLE E ET AL: "Feeder-independent culture of human embryonic stem cells", NATURE METHODS, NATURE PUBLISHING GROUP, GB, vol. 3, no. 8, 1 August 2006 (2006-08-01), pages 637-646, XP008084296, ISSN: 1548-7091, DOI: 10.1038/NMETH902
  • VANESSA DING ET AL: "Deciphering the Importance of Three Key Media Components in Human Embryonic Stem Cell Cultures", BIOTECHNOLOGY LETTERS, SPRINGER NETHERLANDS, DORDRECHT, vol. 28, no. 7, 1 April 2006 (2006-04-01), pages 491-495, XP019231244, ISSN: 1573-6776, DOI: 10.1007/S10529-006-0005-8
  • CHEN JIEKAI ET AL: "Rational optimization of reprogramming culture conditions for the generation of induced pluripotent stem cells with ultra-high efficiency and fast kinetics", CELL RESEARCH, vol. 21, no. 6, June 2011 (2011-06), pages 884-894, XP002695107, ISSN: 1001-0602
   
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