(19)
(11) EP 2 444 807 A3

(12) EUROPEAN PATENT APPLICATION

(88) Date of publication A3:
27.02.2013 Bulletin 2013/09

(43) Date of publication A2:
25.04.2012 Bulletin 2012/17

(21) Application number: 12151524.1

(22) Date of filing: 01.11.2007
(51) International Patent Classification (IPC): 
G01N 33/53(2006.01)
 C07K 16/44(2006.01)
(84) Designated Contracting States:
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC MT NL PL PT RO SE SI SK TR

(30) Priority: 01.11.2006 US 856133 P

(62) Application number of the earlier application in accordance with Art. 76 EPC:
07867341.5 / 2078197

(71) Applicant: Ventana Medical Systems, Inc.
Tucson, Arizona 85755 (US)

(72) Inventors:
  • Kosmeder, Jerry W.
    Tucson, AZ 85750 (US)
  • Lefever, Mark
    Oro Valley, AZ 85737 (US)
  • Johnson, Donald
    Tucson, AZ 85755 (US)
  • Farrell, Michael
    Tucson, AZ 85704 (US)
  • Zhilina, Zhanna
    Oro Valley, AZ 85737 (US)
  • Bieniarz, Christopher
    Tucson, AZ 85718 (US)

(74) Representative: Bond, Christopher William et al
Forresters Skygarden Erika-Mann-Strasse 11
80636 München
80636 München (DE)

   


(54) HAPTENS, HAPTEN CONJUGATES, COMPOSITIONS THEREOF AND METHOD FOR THEIR PREPARATION AND USE


(57) A method for performing a multiplexed diagnostic assay, such as for two or more different targets in a sample, is described. One embodiment comprised contacting the sample with two or more specific binding moieties that bind specifically to two or more different targets. The two or more specific binding moieties are conjugated to different haptens, and at least one of the haptens is an oxazole, a pyrazole, a thiazole, a nitroaryl compound other than dinitrophenyl, a benzofurazan, a triterpene, a urea, a thiourea, a rotenoid, a coumarin, a cyclolignan, a heterobiaryl, an azo aryl, or a benzodiazepine. The sample is contacted with two or more different anti-hapten antibodies that can be detected separately. The two or more different anti-hapten antibodies may be conjugated to different detectable labels.







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