(19)
(11) EP 2 449 113 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
25.11.2015 Bulletin 2015/48

(45) Mention of the grant of the patent:
14.10.2015 Bulletin 2015/42

(21) Application number: 11738625.0

(22) Date of filing: 25.07.2011
(51) International Patent Classification (IPC): 
C12N 15/87(2006.01)
A61K 47/34(2006.01)
A61K 39/39(2006.01)
 A61K 48/00(2006.01)
A61K 47/48(2006.01)
A61K 39/00(2006.01)
(86) International application number:
PCT/EP2011/003719
(87) International publication number:
WO 2012/013326 (02.02.2012 Gazette 2012/05)

(54)

COMPLEXATION OF NUCLEIC ACIDS WITH DISULFIDE-CROSSLINKED CATIONIC COMPONENTS FOR TRANSFECTION AND IMMUNOSTIMULATION

KOMPLEXIERUNG VON NUCLEINSÄUREN MIT DISULFID-VERNETZTEN KATIONISCHEN BESTANDTEILEN ZUR TRANSFEKTION UND IMMUNSTIMULATION

COMPLEXATION D'ACIDES NUCLÉIQUES AVEC DES COMPOSANTS CATIONIQUES RÉTICULÉS PAR DES LIAISONS DISULFURE POUR LA TRANSFECTION ET L'IMMUNOSTIMULATION

(84) Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

(30) Priority: 30.07.2010 US 369549 P
30.07.2010 EP 10007992

(43) Date of publication of application:
09.05.2012 Bulletin 2012/19

(60) Divisional application:
15001874.5

(73) Proprietor: CureVac AG
72076 Tübingen (DE)

(72) Inventors:
  • BAUMHOF, Patrick
    72144 Dusslingen (DE)
  • VOSS, Söhnke
    69151 Neckarsgmünd (DE)
  • KRAMPS, Thomas
    72070 Tübingen (DE)
  • KALLEN, Karl-Josef
    50226 Königsdorf (DE)

(74) Representative: Graf von Stosch, Andreas et al
Graf von Stosch Patentanwaltsgesellschaft mbH Prinzregentenstrasse 22
80538 München
80538 München (DE)


(56) References cited: : 
WO-A1-99/53961
WO-A1-2009/053700
WO-A2-01/04135
 WO-A1-2009/030481
WO-A1-2009/086640
US-B1- 6 689 757
   
  • READ MARTIN L ET AL: "A versatile reducible polycation-based system for efficient delivery of a broad range of nucleic acids", NUCLEIC ACIDS RESEARCH, vol. 33, no. 9, E86, May 2005 (2005-05), pages 1-16, XP002447464, DOI: :10.1093/nar/gni085 cited in the application
  • READ M L ET AL: "Vectors based on reducible polycations facilitate intracellular release of nucleic acids", JOURNAL OF GENE MEDICINE, vol. 5, no. 3, 1 March 2003 (2003-03-01), pages 232-245, XP002481542, WILEY, US ISSN: 1099-498X, DOI: 10.1002/JGM.331 cited in the application
  • MCKENZIE D L ET AL: "A potent new class of reductively activated peptide gene delivery agents", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 275, no. 14, 7 April 2000 (2000-04-07), pages 9970-9977, XP002238140, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, INC, US ISSN: 0021-9258, DOI: 10.1074/JBC.275.14.9970 cited in the application
  • MCKENZIE D L ET AL: "Low molecular weight disulfide cross-linking peptides as nonviral gene delivery carriers.", BIOCONJUGATE CHEMISTRY, vol. 11, no. 6, November 2000 (2000-11), pages 901-909, XP002609379, ISSN: 1043-1802 cited in the application
  • GAO XIANG ET AL: "Nonviral gene delivery: what we know and what is next.", THE AAPS JOURNAL, vol. 9, no. 1, 2007, pages E92-104, XP002609380, ISSN: 1550-7416 cited in the application
  • HARDY JOHN G ET AL: "Synergistic effects on gene delivery--co-formulation of small disulfide-linked dendritic polycations with Lipofectamine 2000.", ORGANIC & BIOMOLECULAR CHEMISTRY, vol. 7, no. 4, 21 February 2009 (2009-02-21), pages 789-793, XP002609381, ISSN: 1477-0539
  • KIM SUN HWA ET AL: "VEGF siRNA delivery system using arginine-grafted bioreducible poly(disulfide amine).", MOLECULAR PHARMACEUTICS, vol. 6, no. 3, May 2009 (2009-05), pages 718-726, XP002609382, ISSN: 1543-8384
  • TAKAE SEIJI ET AL: "PEG-detachable polyplex micelles based on disulfide-linked block catiomers as bioresponsive nonviral gene vectors.", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 130, no. 18, 7 May 2008 (2008-05-07), pages 6001-6009, XP002609383, ISSN: 1520-5126
  • LO ET AL: "An endosomolytic Tat peptide produced by incorporation of histidine and cysteine residues as a nonviral vector for DNA transfection", BIOMATERIALS, vol. 29, no. 15, 4 March 2008 (2008-03-04) , pages 2408-2414, XP022526913, ELSEVIER SCIENCE PUBLISHERS BV., BARKING, GB ISSN: 0142-9612, DOI: 10.1016/J.BIOMATERIALS.2008.01.031
  • MIYATA K ET AL: "Block Catiomer Polyplexes with Regulated Densities of Charge and Disulfide Cross-Linking Directed To Enhance Gene Expression", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, vol. 126, no. 8, 3 March 2004 (2004-03-03) , pages 2355-2361, XP002993261, ISSN: 0002-7863, DOI: 10.1021/JA0379666
  • TOKUNAGA MASAKI ET AL: "Effect of oligopeptides on gene expression: comparison of DNA/peptide and DNA/peptide/liposome complexes.", INTERNATIONAL JOURNAL OF PHARMACEUTICS, vol. 269, no. 1, 9 January 2004 (2004-01-09), pages 71-80, XP002609384, ISSN: 0378-5173
   
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