(19)
(11) EP 2 481 793 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
29.11.2017 Bulletin 2017/48

(45) Mention of the grant of the patent:
30.08.2017 Bulletin 2017/35

(21) Application number: 10819056.2

(22) Date of filing: 21.09.2010
(51) International Patent Classification (IPC): 
C12N 1/21(2006.01)
C12N 15/52(2006.01)
C12P 7/16(2006.01)
(86) International application number:
PCT/KR2010/006511
(87) International publication number:
WO 2011/037415 (31.03.2011 Gazette 2011/13)

(54)

RECOMBINANT MICROORGANISM WITH INCREASED BUTANOL PRODUCTION ABILITY, AND PREPARATION METHOD OF BUTANOL USING SAME

REKOMBINANTER MIKROORGANISMUS MIT ERHÖHTER BUTANOLHERSTELLUNGSFÄHIGKEIT SOWIE HERSTELLUNGSVERFAHREN FÜR BUTANOL DAMIT

MICRO-ORGANISME RECOMBINANT À CAPACITÉ DE PRODUCTION DE BUTANOL ACCRUE, ET PROCÉDÉ DE PRÉPARATION DE BUTANOL L'UTILISANT


(84) Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR

(30) Priority: 22.09.2009 KR 20090089829

(43) Date of publication of application:
01.08.2012 Bulletin 2012/31

(73) Proprietor: GS Caltex Corporation
Seoul 135-985 (KR)

(72) Inventors:
  • LEE, Sang Yup
    Daejeon 305-761 (KR)
  • JANG, Yu-Sin
    Daejeon 305-753 (KR)
  • LEE, Jin Young
    Incheon 405-802 (KR)
  • LEE, Joung Min
    Wonju-si Gangwon-do 220-170 (KR)
  • PARK, Jin Hwan
    Daejeon 305-333 (KR)

(74) Representative: Hiebl, Inge Elisabeth 
Kraus & Weisert Patentanwälte PartGmbB Thomas-Wimmer-Ring 15
80539 München
80539 München (DE)


(56) References cited: : 
EP-A1- 2 267 141
WO-A2-2008/143704
WO-A1-2008/052596
   
  • XIAOGUANG LIU ET AL: "Construction and Characterization of ack Deleted Mutant of Clostridium tyrobutyricum for Enhanced Butyric Acid and Hydrogen Production", BIOTECHNOLOGY PROGRESS, vol. 22, no. 5, 1 January 2006 (2006-01-01), pages 1265-1275, XP55022716, ISSN: 8756-7938, DOI: 10.1021/bp060082g
  • GREEN EDWARD M ET AL: "GENETIC MANIPULATION OF ACID FORMATION PATHWAYS BY GENE ACTIVATION IN CLOSTRIDIUM ACETOBUTYLICUM ATCC 824", MICROBIOLOGY, SOCIETY FOR GENERAL MICROBIOLOGY, READING, GB, vol. 142, no. 8, 1 January 1996 (1996-01-01), pages 2079-2086, XP001248643, ISSN: 1350-0872
  • BOYNTON Z L ET AL: "Cloning, sequencing, and expression of genes encoding phosphotransacetylase and acetate kinase from Clostridium acetobutylicum ATCC 824.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY AUG 1996, vol. 62, no. 8, August 1996 (1996-08), pages 2758-2766, XP002697631, ISSN: 0099-2240
  • SHOTA ATSUMI ET AL: "Metabolic engineering of Escherichia coli for 1-butanol production", METABOLIC ENGINEERING, ACADEMIC PRESS, US, vol. 10, 1 January 2008 (2008-01-01), pages 305-311, XP008156615, ISSN: 1096-7176, DOI: 10.1016/J.YMBEN.2007.08.003 [retrieved on 2007-09-14]
  • LEE SANG YUP ET AL: "Fermentative butanol production by clostridia", BIOTECHNOLOGY AND BIOENGINEERING, WILEY & SONS, HOBOKEN, NJ, US; BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US, vol. 101, no. 2, 1 October 2008 (2008-10-01), pages 209-228, XP002666128, ISSN: 0006-3592, DOI: 10.1002/BIT.22003 [retrieved on 2008-06-09]
  • SCOTCHER, C. M. ET AL.: 'Sequences affecting the regulation of solvent production in Clostridium acetobutylicum.' JOURNAL OF INDUSTRIAL MICROBIOLOGY AND BIOTECHNOLOGY. vol. 30, no. 7, July 2003, pages 414 - 420, XP008151480
  • NAIR, V. R. ET AL.: 'Expression ofplasmid-encoded aad in Clostridium acetobutylicum M5 restores vigorous butanol production.' JOURNAL OF BACTERIOLOGY. vol. 176, no. 18, September 1994, pages 5843 - 5846, XP002486978
  • JIANG, Y. ET AL.: 'Disruption of the acetoacetate decarboxylase gene in solvent-producing Clostridium acetobutylicum increases the butanol ratio.' METABOLIC ENGINEERING. vol. 11, 26 June 2009, pages 284 - 291, XP026439231
  • GIRBAL, L. ET AL.: 'Regulation of solvent production in clostridium acetobutylicum.' TRENDS IN BIOTECHNOLOGY. vol. 16, no. 1, January 1998, pages 11 - 16, XP004101891
  • Ryan Sillers ET AL: "Aldehyde-alcohol dehydrogenase and/or thiolase overexpression coupled with CoA transferase downregulation lead to higher alcohol titers and selectivity in Clostridium acetobutylicum fermentations", Biotechnology and Bioengineering, vol. 102, no. 1, 1 January 2009 (2009-01-01), pages 38-49, XP55044193, ISSN: 0006-3592, DOI: 10.1002/bit.22058
  • NAIR R V ET AL: "MOLECULAR CHARACTERIZATION OF AN ALDEHYDE/ALCOHOL DEHYDROGENASE GENE FROM CLOSTRIDIUM ACETOBUTYLICUM ATCC 824", JOURNAL OF BACTERIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 176, no. 3, 1 February 1994 (1994-02-01), pages 871-885, XP002045431, ISSN: 0021-9193
   
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