(19)
(11) EP 2 519 636 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
09.08.2017 Bulletin 2017/32

(45) Mention of the grant of the patent:
01.03.2017 Bulletin 2017/09

(21) Application number: 10841650.4

(22) Date of filing: 28.12.2010
(51) International Patent Classification (IPC): 
C12N 15/45(2006.01)
A61K 39/17(2006.01)
(86) International application number:
PCT/US2010/062274
(87) International publication number:
WO 2011/082189 (07.07.2011 Gazette 2011/27)

(54)

PRODUCTION OF HEMAGGLUTININ-NEURAMINIDASE PROTEIN IN MICROALGAE

HERSTELLUNG EINES HÄMAGGLUTININ-NEURAMINIDASE-PROTEINS IN MIKROALGEN

PRODUCTION DE PROTÉINE HÉMAGGLUTININE-NEURAMINIDASE DANS DES MICROALGUES


(84) Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

(30) Priority: 28.12.2009 US 290469 P

(43) Date of publication of application:
07.11.2012 Bulletin 2012/45

(73) Proprietors:
  • DSM IP Assets B.V.
    6411 TE Heerlen (NL)
  • Merial, Inc.
    Duluth, GA 30096 (US)

(72) Inventors:
  • APT, Kirk E.
    Ellicott City, Maryland 21042 (US)
  • LIPPMEIER, James Casey
    Columbia, Maryland 21045 (US)
  • BAYNE, Anne-Cecile V.
    Ellicott City, Maryland 21043 (US)
  • PRITCHARD, Joyce A.
    Gainesville, Georgia 30504 (US)
  • GUO, Xuan
    Suwanee, Georgia 30024 (US)

(74) Representative: D Young & Co LLP 
120 Holborn
London EC1N 2DY
London EC1N 2DY (GB)


(56) References cited: : 
EP-A1- 2 090 648
WO-A2-2006/013572
US-A1- 2006 275 904
US-A1- 2007 128 222
WO-A2-02/083869
US-A1- 2003 166 207
US-A1- 2006 286 650
US-A1- 2008 022 422
   
  • PRASAD V ET AL: "Expression of biologically active Hemagglutinin-neuraminidase protein of Peste des petits ruminants virus in transgenic pigeonpea [Cajanus cajan (L) Millsp.]", PLANT SCIENCE, ELSEVIER IRELAND LTD, IE, vol. 166, no. 1, 1 January 2004 (2004-01-01), pages 199-205, XP008161290, ISSN: 0168-9452
  • GOMEZ E ET AL: "Expression of Hemagglutinin-Neuraminidase glycoprotein of Newcastle Disease Virus in agroinfiltrated Nicotiana benthamiana plants", JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 144, no. 4, 1 December 2009 (2009-12-01), pages 337-340, XP026778485, ISSN: 0168-1656, DOI: 10.1016/J.JBIOTEC.2009.09.015 [retrieved on 2009-09-30]
  • TANABAYASHI ET AL.: "Expression of mumps virus glycoproteins in mammalian cells from cloned cDNAs: both F and HN proteins are required for cell fusion.", VIROLOGY, vol. 187, no. 2, April 1992 (1992-04), pages 801-804, XP0026462300,
  • LEÓN ROSA ET AL: "Nuclear transformation of eukaryotic microalgae: historical overview, achievements and problems", ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY,, vol. 16, 1 January 2007 (2007-01-01), pages 1-11, XP009105948, ISSN: 0065-2598, DOI: 10.1007/978-0-387-75532-8_1
  • LEON-BANARES R ET AL: "Transgenic microalgae as green cell-factories", TRENDS IN BIOTECHNOLOGY, ELSEVIER PUBLICATIONS, CAMBRIDGE, GB, vol. 22, no. 1, 1 January 2004 (2004-01-01), pages 45-52, XP004481957, ISSN: 0167-7799, DOI: 10.1016/J.TIBTECH.2003.11.003
  • ANNE-CÉCILE V. BAYNE ET AL: "Vaccination against Influenza with Recombinant Hemagglutinin Expressed by Schizochytrium sp. Confers Protective Immunity", PLOS ONE, vol. 8, no. 4, 1 January 2013 (2013-01-01) , pages e61790-e61790, XP055062273, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0061790
  • TANABAYASHI ET AL.: 'Expression of mumps virus glycoproteins in mammalian cells from cloned cDNAs: both F and HN proteins are required for cell fusion.' VIROLOGY vol. 187, no. 2, April 1992, pages 801 - 804
  • SIM ET AL.: 'Expression and Characterization of Synthetic Heat-Labile Enterotoxin B Subunit and Hemagglutinin?Neuraminidase-Neutralizing Epitope Fusion Protein in Escherichia coli and Tobacco Chloroplasts.' PLANT MOL BIOL REP vol. 27, 28 May 2009, pages 388 - 399, XP008161281
  • FUHRMANN ET AL.: 'Production of Antigens in Chlamydomonas reinhardtii: Green Microalgae as a Novel Source of Recombinant Proteins.' FROM: METHODS IN MOLECULAR MEDICINE, VOL. 94: MOLECULAR DIAGNOSIS OF INFECTIOUS DISEASES. vol. 94, 2004, pages 191 - 195
  • SIRIPORNADULSIL ET AL.: 'CHAPTER 11: Microalgal Vaccines. from Transgenic Microalgae as Green Cell Factories.' LANDES BIOSCIENCE AND SPRINGER SCIENCE BUSINESS MEDIA. 2007, pages 122 - 128
  • FLOSS ET AL.: 'Production of vaccines and therapeutic antibodies for veterinary applications in transgenic plants: an overview.' TRANSGENIC RES vol. 16, 2007, pages 315 - 332
  • JOENSUU ET AL.: 'Transgenic plants for animal health: plant-made vaccine antigens for animal infectious disease control.' PHYTOCHEM REV vol. 7, 2008, pages 553 - 577, XP019613381
  • PRASAD ET AL.: 'Expression of biologically active Hemagglutinin-neuraminidase protein of Peste des petits ruminants virus in transgenic pigeonpea [Cajanus cajan (L) Millsp.]' PLANT SCIENCE. vol. 166, 2004, pages 199 - 205, XP008161290
   
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