(19)

(11)

EP 2 556 146 A2

(12)

(88) Date of publication A3: 29.12.2011 (43) Date of publication: 13.02.2013 Bulletin 2013/07 (21) Application number: 10849173.9 (22) Date of filing: 30.12.2010

(51) International Patent Classification (IPC):  C12N 5/076(2010.01) C12Q 1/02(2006.01) C12N 5/02(2006.01) G01N 33/48(2006.01) (86) International application number: PCT/US2010/062598 (87) International publication number: WO 2011/123166 (06.10.2011 Gazette 2011/40)

(84) Designated Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR (30) Priority: 01.04.2010 US 320183 P 14.04.2010 US 624192 P 28.10.2010 WO PCT/US2010/054549 (71) Applicant: Inguran, LLC Navasota, TX 77868 (US)

(72) Inventors: MORENO, Juan College Station Texas 77840 (US) EVANS, Michael College Station Texas 77845 (US) KJELLAND, Michael Valley City North Dakota 58072 (US) GOSALVEZ, Jamie E-28770 Colmenar Viejo (ES) GONZALEZ MARTIN, Clara Navasota Texas 77868 (US) LOPEZ FERNANDEZ, Carmen E-28770 Colmenar Viejo (ES) (74) Representative: Jacob, Reuben Ellis et al R.G.C. Jenkins & Co 26 Caxton Street London SW1H 0RJ London SW1H 0RJ (GB)