(19)

(11)

EP 2 571 978 A2

(12)

(88) Date of publication A3: 05.01.2012 (43) Date of publication: 27.03.2013 Bulletin 2013/13 (21) Application number: 11782806.1 (22) Date of filing: 18.05.2011

(51) International Patent Classification (IPC):  C12N 1/12(2006.01) C12M 1/00(2006.01) C12M 1/42(2006.01) C12N 1/00(2006.01) C12N 5/04(2006.01) A01G 33/00(2006.01) C12M 1/04(2006.01) C12M 3/00(2006.01) C12N 1/20(2006.01) C12Q 3/00(2006.01) (86) International application number: PCT/CA2011/000574 (87) International publication number: WO 2011/143749 (24.11.2011 Gazette 2011/47)

(84) Designated Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR (30) Priority: 29.04.2011 CA 2738397 29.04.2011 CA 2738516 29.04.2011 CA 2738418 29.04.2011 CA 2738410 29.04.2011 CA 2738461 29.04.2011 CA 2738459 07.02.2011 US 201113022396 20.05.2010 US 784106 20.05.2010 US 784126 20.05.2010 US 784141 20.05.2010 US 784172 20.05.2010 US 784181 20.05.2010 US 784215 (71) Applicant: Pond Biofuels Inc. Scarborough, Ontario M1E 3S4 (CA)

(72) Inventors: GONZALEZ, Jaime, A. Oakville Ontario L6M 0C8 (CA) KOLESNIK, Max Toronto Ontario M2J 2T1 (CA) MARTIN, Steven, C. Toronto Ontario M4J 1P6 (CA) DIPIETRO, Tony Brampton Ontario L6Z 1R1 (CA) DIPIETRO, Emidio Brampton Ontario L7A 2T8 (CA) (74) Representative: Trinks, Ole et al Meissner, Bolte & Partner GbR Widenmayerstrasse 48 80538 München 80538 München (DE)