(19)
(11) EP 2 668 273 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
15.03.2017 Bulletin 2017/11

(45) Mention of the grant of the patent:
06.04.2016 Bulletin 2016/14

(21) Application number: 12707675.0

(22) Date of filing: 30.01.2012
(51) International Patent Classification (IPC): 
C12N 15/10(2006.01)
 C12Q 1/68(2006.01)
(86) International application number:
PCT/US2012/023139
(87) International publication number:
WO 2012/103545 (02.08.2012 Gazette 2012/31)

(54)

OLIGONUCLEOTIDE REPLACEMENT FOR DI-TAGGED AND DIRECTIONAL LIBRARIES

OLIGONUKLEOTIDERSATZ FÜR DI-MARKIERTE UND GERICHTETE BIBLIOTHEKEN

REMPLACEMENT D'OLIGONUCLÉOTIDES POUR BIBLIOTHÈQUES MARQUÉES AUX DEUX EXTRÉMITÉS ET DIRECTIONNELLES

(84) Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

(30) Priority: 28.01.2011 US 201161437451 P
12.07.2011 US 201161506777 P

(43) Date of publication of application:
04.12.2013 Bulletin 2013/49

(60) Divisional application:
16155567.7 / 3037536

(73) Proprietor: Illumina, Inc.
San Diego, CA 92122 (US)

(72) Inventors:
  • GORYSHIN, Igor
    San Diego California 92122 (US)
  • BAAS, Bradley
    San Diego California 92122 (US)
  • VAIDYANATHAN, Ramesh
    San Diego California 92122 (US)
  • MAFFITT, Mark
    San Diego California 92122 (US)

(74) Representative: Cooley (UK) LLP 
Dashwood 69 Old Broad Street
London EC2M 1QS
London EC2M 1QS (GB)


(56) References cited: : 
US-A- 5 925 545
 US-A1- 2010 120 098
   
  • ADEY ANDREW ET AL: "Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition", 2010, GENOME BIOLOGY, VOL. 11, NR. 12, PAGE(S) ARTICLE NO.: R119, XP002676821, ISSN: 1474-7596(print) page 1, column 2, line 22 - page 2, column 1, line 13 page 2, column 2, lines 5-10; figure 2 page 3, column 2, line 3 - page 5, column 1, paragraph 1 page 9, column 2, paragraph 2 page 10, column 2, paragraph 2 page 11, column 1, line 11, last paragraph - page 11, column 2, paragraph 1 page 12, column 1, last paragraph - page 13, column 1, paragraph 2 page 13, column 1, paragraph 2 - page 14, column 2, paragraph 2
  • SYED FRAZ ET AL: "Optimized library preparation method for next-generation sequencing", NATURE METHODS, NATURE PUBLISHING GROUP, GB, vol. 6, no. 10, 1 October 2009 (2009-10-01), pages I-II, XP002672202, ISSN: 1548-7091
  • CARUCCIO NICHOLAS: "Preparation of next-generation sequencing libraries using Nextera(TM) technology: simultaneous DNA fragmentation and adaptor tagging by in vitro transposition", METHODS IN MOLECULAR BIOLOGY, HUMANA PRESS, vol. 733, 1 January 2011 (2011-01-01), pages 241-255, XP009157814, ISSN: 1940-6029, DOI: 10.1007/978-1-61779-089-8_17 [retrieved on 2011-01-01]
  • GERTZ JASON ET AL: "Transposase mediated construction of RNA-seq libraries", GENOME RESEARCH, vol. 22, no. 1, January 2012 (2012-01), pages 134-141, XP009159746,
   
Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).