(19)
(11) EP 2 677 039 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
05.10.2022 Bulletin 2022/40

(45) Mention of the grant of the patent:
17.08.2022 Bulletin 2022/33

(21) Application number: 13150067.0

(22) Date of filing: 10.05.2007
(51) International Patent Classification (IPC): 
C12Q 1/6827(2018.01)
C12Q 1/6858(2018.01)
C12Q 1/6818(2018.01)
(52) Cooperative Patent Classification (CPC):
C12Q 1/6818; C12Q 1/6827; C12Q 1/6858; Y10T 436/143333
 
C-Sets:
  1. C12Q 1/6818, C12Q 2523/109, C12Q 2533/107, C12Q 2565/514;
  2. C12Q 1/6827, C12Q 2523/109, C12Q 2533/107, C12Q 2565/514;
  3. C12Q 1/6858, C12Q 2523/109, C12Q 2533/107, C12Q 2565/514;


(54)

Detection of nucleic acid targets using chemically reactive oligonucleotide probes

Erkennung von Nukleinsäuretargets mithilfe chemisch reaktiver Oligonnucleotidsonden

Détection de cibles d'acides nucléiques au moyen de sondes oligonucléotidiques chimiquement réactives


(84) Designated Contracting States:
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC MT NL PL PT RO SE SI SK TR

(30) Priority: 10.05.2006 US 746897 P

(43) Date of publication of application:
25.12.2013 Bulletin 2013/52

(62) Application number of the earlier application in accordance with Art. 76 EPC:
07794804.0 / 2021514

(73) Proprietor: DxTerity Diagnostics Incorporated
Rancho Dominguez, CA 90220 (US)

(72) Inventor:
  • Terbrueggen, Robert
    Manhattan Beach, CA 90266 (US)

(74) Representative: Mewburn Ellis LLP 
Aurora Building Counterslip
Bristol BS1 6BX
Bristol BS1 6BX (GB)


(56) References cited: : 
WO-A-89/12696
WO-A1-01/66802
WO-A-2004/076692
US-A- 5 681 943
   
  • HIROSHI ABE ET AL: "Destabilizing Universal Linkers for Signal Amplification in Self-Ligating Probes for RNA", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 126, no. 43, 1 November 2004 (2004-11-01), pages 13980-13986, XP55019411, ISSN: 0002-7863, DOI: 10.1021/ja046791c
  • GRYAZNOV AND R L LETSINGER S M: "Chemical Ligation of Oligonucleotides in the Presence and Absence of a Template", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC, US, no. 115, 5 May 1993 (1993-05-05), pages 3808-9, XP002073065, ISSN: 0002-7863
  • XU Y ET AL: "High sequence fidelity in a non-enzymatic DNA autoligation reaction", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 27, no. 3, 1 February 1999 (1999-02-01), pages 875-881, XP002225841, ISSN: 0305-1048
  • LI JINGHUAN ET AL: "Comparison of miRNA expression patterns using total RNA extracted from matched samples of formalin-fixed paraffin-embedded (FFPE) cells and snap frozen cells", BMC BIOTECHNOLOGY, BIOMED CENTRAL LTD. LONDON, GB, vol. 7, no. 1, 29 June 2007 (2007-06-29), page 36, XP021029476, ISSN: 1472-6750, DOI: 10.1186/1472-6750-7-36
   
Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).