(19)
(11) EP 2 719 697 A1

(12) EUROPEAN PATENT APPLICATION
published in accordance with Art. 153(4) EPC

(43) Date of publication:
16.04.2014 Bulletin 2014/16

(21) Application number: 12797048.1

(22) Date of filing: 04.06.2012
(51) International Patent Classification (IPC): 
C07D 471/14(2006.01)
A61K 31/4375(2006.01)
A61P 35/00(2006.01)
A61P 17/00(2006.01)
 C07D 519/00(2006.01)
A61K 9/00(2006.01)
A61P 13/08(2006.01)
(86) International application number:
PCT/CN2012/000761
(87) International publication number:
WO 2012/167606 (13.12.2012 Gazette 2012/50)
(84) Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

(30) Priority: 04.06.2011 CN 201110159903
04.11.2011 CN 201110365829

(71) Applicant: Xuanzhu Pharma Co., Ltd.
Jinan Shandong 250101 (CN)

(72) Inventors:
  • WU, Frank
    Jinan Shandong 250101 (CN)
  • ZHANG, Yan
    Jinan Shandong 250101 (CN)

(74) Representative: Croce, Valeria et al
Jacobacci & Partners S.p.A. Via Senato, 8
20121 Milano
20121 Milano (IT)

   


(54) PYRIDONAPHTHYRIDINE PI3K/MTOR DUAL INHIBITORS AND PREPARATION AND USE THEREOF


(57) The present invention relates to a pyridonaphthyridine compound as represented by general formula (I), which has a dual PI3K and mTOR inhibition effect, and its pharmaceutically acceptable salt, stereoisomer and deuteride thereof, wherein R1, R2, R3, R4, R5, R6, R7 and X are as defined in the specification; the present invention also relates to a method for preparing said compound, a pharmaceutical composition and a pharmaceutical formulation containing said compound, and uses of said compound in treating and/or preventing a proliferative disease and in the manufacture of a medicament for treating and/or preventing a proliferative disease.




Description

Technical Field



[0001] The present invention relates to a pyridonaphthyridine compound, which has a dual PI3K and mTOR inhibition effect, and its pharmaceutically acceptable salt, stereoisomer and deuteride thereof; a preparation method of said compound, a pharmaceutical composition and a pharmaceutical formulation containing said compound, and uses of said compound in treating and/or preventing a proliferative disease and in the manufacture of a medicament for treating and/or preventing a proliferative disease.

Background



[0002] Tumor is a neoplasma formed in the organism under various oncogenic factors, bringing about the change in the cell genetic material, and leading to the abnormal gene expression and the cell abnormal proliferation. Tumor cells lose the normal growth regulation function, has an autonomic or a relative autonomic growth capability. Even if the oncogenic factor disappears, the tumor cells can continue to grow and consume a large amount of nutrient substances in the human body. If finding and treating the tumor is not prompt, the tumor cells may also transfer to various places in the whole body to grow and propagate, and release many toxins, leading to maransis, anaemia, organ function impairment and even the death of the human being.

[0003] The therapy for treating tumor mainly comprises the following three aspects: medicament therapy, surgical therapy and radiation therapy. Since it is difficult for the surgical therapy and the radiation therapy to eradicate the tumor thoroughly, and the surgical therapy and the radiation therapy have little function to the patients in the middle-late stage, therefore the medicament therapy becomes more and more important in the tumor treatment. Traditional antineoplastic drug cannot distinguish the tumor cells from the normal tissue cells, leading to a severe side effect. The targeted drug aims at the cancer cells as the specific target, can accurately target to the tumor, and accordingly improves the therapeutic level greatly and decreases the adverse effect ratio. For example, it can increase the median live time for the late-stage large intestine carcinoma by 66.7%, and the treatment effective rate for the late-stage mammary cancer by 71.3%.

[0004] Because many pharmaceuticals company has accelerated the research and development in the targeted antineoplastic drug, plus because there is a strong requirement on this kind of the antineoplastic drug in the commercial market, the molecular targeted drug has became a fastest growth unit in the global antineoplastic drug market. PI3K pathway is a place where the human tumor cells are subjected to variation most frequently, which may lead to the cell proliferation, activation, and amplification of signal. Phosphatidylinositol-3-kinase (PI3K) and mammalian target of rapamycin (mTOR) are the most important kinases for the PI3K signaling pathway.

[0005] Phosphatidylinositol-3-kinase (PI3K) refers to a member of the lipid kinase family, which can produce the phosphatidylinositol 3-phosphate (PIP3) through the 3-position phosphorylation of phosphatidylinositol to regulate the cell metabolism and growth. The second messenger PIP3 of this kind may cause the P13K and the downstream effector (in particular AKt) to combine in pair, leading to membrane recruitment and phosphorylation, cell proliferation and activation. Therefore, the inhibition of Phosphatidylinositol-3-kinase may have an influence on the PI3K pathway, and accordingly inhibit the cancer cell proliferation and activation.

[0006] The mTOR is a serine/threonine protein kinase present in kytoplasm, belongs to phosphoinositide kinase dependent protein kinase family, and present in the organism in a form of two complexes, i.e. mTORC1 (the target point for rapamycin) and mTORC2 (not inhibited by rapamycin). The mTOR is a cell signal transducer. It regulates the response of the tumor cells to the nutrients and growth factors, and controls the blood supply to the tumor through the effect on vascular endothelial growth factor. The mTOR inhibitor can make the tumor cells starve and inhibit the mTOR, leading to the decrease in the tumor volume. Currently, there is some drug development in the dual PI3K and mTOR inhibitor. However, most of such compounds have poor druggability, and this kind of compounds has not entered the pharmaceutical market. Journal of Medicinal Chemistry (2011), 54(5), 1473-1480, "Discovery of 9-(6-Aminopyridin-3-yl)-1-(3-(trifluoromethyl)phenyl)benzo[h][1,6]naphthyridin-2(1H)-one (Torin2) as a potent, selective, and orally available mammalian target of rapamycin (mTOR) inhibitor for treatment of cancer" discloses a compound named Torin2 and reports the research results on its in vivo pharmacokinetics.



[0007] In summary, it has became hot in the current research for the antineoplastic drug to seek a compound having a dual PI3K and mTOR inhibition, a good activity, a high selectivity, and a good pharmacokinetic characteristics.

[0008] The present inventors, upon developing the drug with the good antineoplastic effect, have found a class of pyridonaphthyridine compound having a dual PI3K and mTOR inhibition.

Summary of the Invention



[0009] Thus, the present invention provides a compound represented by general formula (I), and its pharmaceutically acceptable salt, stereoisomer and deuteride thereof:

wherein:

X is O;

R1 is hydrogen or the following group that is unsubstituted or substituted by 1-3 R8: C1-6alkyl, C2-6alkenyl, C2-6alkynyl, 3-14-membered cycloalkyl,

6-14-membered aryl, 3-14-membered heterocyclyl, 7-12-membered spiro ring group or 7-12-membered bridged ring group;

R2 is hydrogen or the following group that is unsubstituted or substituted by 1-3 R8': C1-6alkyl, C2-6alkenyl, C2-6alkynyl, 3-14-membered cycloalkyl,

6-14-membered aryl, 3-14-membered heterocyclyl, 7-12-membered spiro ring group or 7-12-membered bridged ring group;

each of R3 and R4 is independently selected from the group consisting of hydrogen, halogen, cyano, amino, hydroxy, sulfonyl, -SO2C1-6alkyl, and C1-6alkyl and C1-6alkoxyl that are unsubstituted or substituted with 1-3 substituents selected from halogen, hydroxy and/or carboxyl;

R5 is selected from the group consisting of hydrogen, cyano, amino, sulfonyl, -SO2C1-6alkyl, and C1-6alkyl and C1-6alkoxyl that are unsubstituted or substituted with 1-3 substituents selected from halogen, hydroxy and/or carboxyl;

each of R6 and R7 is independently selected from the group consisting of hydrogen, hydroxy, halogen, amino, and C1-6alkyl and C1-6alkoxyl that are unsubstituted or substituted with 1-3 substituents selected from halogen, hydroxy and/or carboxyl;

each of R8 and R8' are independently selected from the group consisting of

  1. (1) hydroxy, halogen, amino, cyano, -(CH2)nNRaRb, -(CH2)nC(O)Rc, -(CH2)nS(O)mRc, -(CH2)nS(O)mNRaRb, -(CH2)nNRaS(O)mRc, -(CH2)nC(O)(CH2)nNRaRb, -(CH2)nOC(O)Rc, -(CH2)nNRaC(O)Rc, and -(CH2)nNRaC(O)NRaRb,
  2. (2) C1-6alkyl, C2-6alkenyl, C2-6alkynyl and C1-6alkoxyl, which are unsubstituted or substituted by 1-3 substitutents selected from cyano, halogen and/or hydroxy, and
  3. (3) 3-14-membered cycloalkyl, 6-14-membered aryl and 3-14-membered heterocyclyl, which are unsubstituted or substituted by 1-3 substitutents selected from cyano, trifluoromethyl, halogen, C1-6alkyl, C2-8alkenyl, C2-8alkynyl, C1-6alkoxyl, -(CH2)nNRaRb, -(CH2)nC(O)Rc', -(CH2)nC(O)(CH2)nNRaRb, -(CH2)nS(O)mRc', -(CH2)nS(O)mNRaRb, -(CH2)nNRaS(O)mRc', -(CH2)nOC(O)Rc', -(CH2)nNRaC(O)Rc' and/or -(CH2)nNRaC(O)NRaRb;

wherein

each of Ra and Rb is independently selected from the group consisting of hydrogen, or C1-6alkyl that is unsubstituted or substituted by 1-3 substitutents selected from hydroxy, halogen and/or cyano;

Rc is C1-6alkyl or C1-6alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from hydroxy, halogen, cyano and/or trifluoromethyl; Rc' is C1-6alkyl, C1-6alkoxyl, 3-8-membered monocyclic cycloalkyl or 3-8-membered monocyclic heterocyclyl, all of which are unsubstituted or substituted by 1-3 substitutents selected from hydroxy, halogen and/or cyano;

m is 0, 1 or 2; and

n is 0-4.



[0010] The present invention also provides a pharmaceutical composition containing the compound of general formula (I), and its pharmaceutically acceptable salt, stereoisomer or deuteride thereof.

[0011] The present invention also provides a pharmaceutical formulation containing the compound of general formula (I), and its pharmaceutically acceptable salt, stereoisomer or deuteride thereof, and a pharmaceutically acceptable carrier.

[0012] The present invention also provides the compound of general formula (I), and its pharmaceutically acceptable salt, stereoisomer or deuteride thereof, and a pharmaceutical composition containing the compound of general formula (I), and pharmaceutically acceptable salt, stereoisomer or deuteride thereof, as a medicament for treating a proliferative disease.

[0013] The present invention also provides a use of the compound of general formula (I), and its pharmaceutically acceptable salt, stereoisomer or deuteride thereof, and a pharmaceutical composition containing the compound of general formula (I), and pharmaceutically acceptable salt, stereoisomer or deuteride thereof in the manufacture of a medicament for treating a proliferative disease.

[0014] The present invention also provides a method of treating a proliferative disease, comprising the step of administrating a subject in need thereof an effective amount of the compound of general formula (I), and its pharmaceutically acceptable salt, stereoisomer or deuteride thereof, or a pharmaceutical composition containing the compound of general formula (I), and its pharmaceutically acceptable salt, stereoisomer or deuteride thereof.

[0015] The present invention also provides a preparation method of the compound of general formula (I), i.e.

comprising the following steps:

wherein R1, R2, R3, R4, R5, R6 and R7 are defined as hereinbefore, Hal1, Hal2 and Hal3 represent halogen, which is each independently selected from the group consisting of F, Cl, Br and I, and Hal1, Hal2 and Hal3 can be identical or different; Alk represents a lower alkyl, e.g. "C1-6alkyl", preferably "C1-4alkyl" and more preferably ethyl; "anhydride" is preferably an organic acid anhydride, for example, selected from but not limited to acetic anhydride, propionic anhydride, preferably acetic anhydride;


1. Preparation of Intermediate 1



[0016] Starting Material 1 and Starting Material 2 are reacted under heating to reflux in an alcohol organic solvent in presence of a base until the starting material disappears to produce Intermediate 1;

2. Preparation of Intermediate 2'



[0017] Intermediate 1 is reacted with an reductant in an alcohol organic solvent ; the solvent is removed under a reduced pressure; water is added to the reaction mixture; the resulting mixture is extracted with a halogenated hydrocarbon organic solvent; the organic phase is concentrated, to which is added an oxidant; the resulting mixture is reacted under stirring to produce Intermediate 2';

3. Preparation of Intermediate 2



[0018] In the nitrogen protection, Intermediate 2' and a Grignard reagent R6-Mg-Hal3 are reacted and then oxidized to produce Intermediate 2;

4. Preparation of Intermediate 3



[0019] 

Method 1: In a sealed vessel, Intermediate 2 and Starting Material 3 are reacted in an alcohol organic solvent in the presence of an inorganic base at 110-180°C to produce Intermediate 3; or

Method 2: Intermediate 2 is dissolved in a non-protonic polar organic solvent and Starting Material 3'; the reaction is conducted in a microwave reactor until the starting material disappears to produce Intermediate 3;
and


5. Preparation of Compound of Formula (I)



[0020] Intermediate 3 and Starting Material 4 are dissolved in an organic solvent; the resulting mixture was reacted in the presence of a catalyst and a base under reflux in a nitrogen-protecting atmosphere to produce the compound of formula (I);

[0021] If necessary, a functional group that needs to be protected, such as hydroxy, amino and the like, can be protected; and afterwards can be deprotected according to the conventional method.

[0022] In the above preparation method,

[0023] Said "alcohol organic solvent" is selected from, for example, but not limited to, methanol, ethanol, isopropanol, t-butanol and the like, preferably ethanol, t-butanol;

[0024] Said "organic solvent" is selected from said "alcohol organic solvent ", an "aromatic hydrocarbon organic solvent" or a mixture thereof, said "aromatic hydrocarbon organic solvent" includes but is not limited to, for example, benzene, toluene, xylene and the like, preferably toluene;

[0025] Said "halogenating hydrocarbon organic solvent" is selected from, for example, but not limited to, chlorobenzene, dichlorobenzene, chloromethane, dichlormethane and the like, preferably dichlormethane;

[0026] Said "base" includes an organic base and an inorganic base, preferably an inorganic base. Said inorganic base is selected from, for example, but not limited to potassium hydroxide, sodium hydroxide, zinc hydroxide, calcium hydroxide, potassium carbonate, potassium bicarbonate, sodium carbonate, sodium bicarbonate and the like, preferably potassium carbonate and sodium carbonate; said organic base includes but is not limited to, for example, triethylamine, ethylene diamine, ethanolamine, diethanolamine, triethanolamine, sodium ethoxide, pyridine, dimethylaminopyridine, sodium methoxide, potassium ethoxide, potassium tertbutoxide, butyl lithium, phenyl lithium, lithium diisopropylamide, lithium hexamethyldisilazide and the like;

[0027] Said "non protonic polar organic solvent " is selected from, for example, but not limited to, N,N-dimethylacetamide, N,N-dimethyl formamide, dimethyl sulfoxide, acetonitrile and the like, preferably N,N-dimethylacetamide;

[0028] Said "reductant" is preferably metal hydrides, is selected from, for example, but not limited to sodium borohydride, lithium aluminum hydride, diborane and the like, preferably sodium borohydride;

[0029] Said "oxidant" preferably is a high valency oxidant which comprises a varying valency element, and selected from, for example, but not limited to potassium permanganate, potassium chlorate, manganese dioxide, ferric chloride and the like, preferably manganese dioxide; and

[0030] Said "catalyst" is selected from, for example, but not limited to, nickel catalyst, palladium catalyst, platinum catalyst, metal hydride catalyst and the like, preferably palladium catalyst, such as Pd/C, palladium chloride, palladium tetrakis(triphenylphosphine) and the like.

[0031] In a preferable embodiment of the present compound of general formula (I), R1 is 3-14-membered cycloalkyl, 6-14-membered aryl, 3-14-membered heterocyclyl, 7-12-membered spiro ring group or 7-12-membered bridged ring group, all of which are unsubstituted or substituted by 1-3 R8, wherein R8 is selected from the group consisting of
  1. (1) hydroxy, halogen, amino, cyano, -(CH2)nNRaRb, -(CH2)nC(O)Rc, -(CH2)nS(O)mRc, -(CH2)nS(O)mNRaRb, -(CH2)nNRaS(O)mRc, -(CH2)nC(O)(CH2)nNRaRb, -(CH2)nOC(O)Rc, -(CH2)nNRaC(O)Rc and -(CH2)nNRaC(O)NRaRb,
  2. (2) C1-6alkyl and C1-6alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from cyano, halogen and/or hydroxy, and
  3. (3) 3-8-membered monocyclic cycloalkyl, 6-14-membered aryl and 3-8-membered monocyclic heterocyclyl, all of which are unsubstituted or substituted by 1-3 substitutents selected from cyano, trifluoromethyl, halogen, C1-6alkoxyl, -(CH2)nNRaRb, -(CH2)nC(O)Rc', -(CH2)nC(O)(CH2)nNRaRb, -(CH2)nS(O)mRc', -(CH2)nS(O)mNRaRb, -(CH2)nNRaS(O)mRc', -(CH2)nOC(O)Rc', -(CH2)nNRaC(O)Rc' and/or -(CH2)nNRaC(O)NRaRb,
wherein

each of Ra and Rb is independently selected from the group consisting of hydrogen, or C1-6alkyl that is unsubstituted or substituted by 1-3 substitutents selected from hydroxy, halogen and/or cyano,

Rc is C1-6alkyl or C1-6alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from hydroxy, halogen, cyano and/or trifluoromethyl, Rc' is C1-6alkyl and C1-6alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from hydroxy, halogen and/or cyano,

m is 0, 1 or 2, and

n is 0, 1, 2 or 3.



[0032] In a further preferable embodiment of the present compound of general formula (I), R1 is 3-8-membered monocyclic cycloalkyl, 6-14-membered aryl or 3-8-membered monocyclic heterocyclyl that are unsubstituted or substituted by 1-3 R8, wherein R8 is selected from the group consisting of
  1. (1) hydroxy, halogen, amino, cyano, -(CH2)nC(O)Rc and -(CH2)nS(O)mRc, wherein Rc is C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by hydroxy, halogen, cyano or trifluoromethyl,
  2. (2) C1-4alkyl and C1-4alkoxyl, which are unsubstituted or substituted by cyano, hydroxy or 1-3 halogens, and
  3. (3) 5-8-membered monocyclic heterocyclyl that is unsubstituted or substituted by 1-3 substitutents selected from cyano, trifluoromethyl, halogen, C1-4alkoxyl, -(CH2)nNRaRb, -(CH2)nC(O)Rc', -(CH2)nC(O)(CH2)nNRaRb and/or -(CH2)nS(O)mRc, wherein each of Ra and Rb is independently selected from the group consisting of hydrogen, or C1-4alkyl that is unsubstituted or substituted by 1-3 substitutents selected from hydroxy, halogen and/or cyano, Rc' is C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from hydroxy, halogen and/or cyano,

    m is 0, 1 or 2, and

    n is 0, 1, 2 or 3.



[0033] In a further preferable embodiment of the present compound of general formula (I), R1 is 6-10-membered aryl or 3-8-membered saturated monocyclic heterocyclyl or 5-6-membered aromatic monocyclic heterocyclyl, all of which are unsubstituted or substituted by 1-3 R8, wherein R8 is selected from the group consisting of
  1. (1) hydroxy, halogen, amino, cyano and -C(O)Rc, wherein Rc is C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by hydroxy, halogen, cyano or trifluoromethyl,
  2. (2) C1-4alkyl and C1-4alkoxyl, which are unsubstituted or substituted by cyano, hydroxy or 1-3 halogens, and
  3. (3) 5-6-membered monocyclic heterocyclyl, which is unsubstituted or substituted by 1-3 substitutents selected from cyano, trifluoromethyl, halogen, C(O)Rc' and/or -S(O)2Rc', wherein Rc' is C1-4alkyl or C1-4alkoxyl, which are unsubstituted or substituted by 1-3 substitutents selected from hydroxy, halogen and/or cyano.


[0034] In a further preferable embodiment of the present compound of general formula (I), R1 is 6-10-membered aryl or 5-6-membered monocyclic heterocyclyl that are unsubstituted or substituted by 1-3 R8, wherein R8 is (1) amino or -C(O)Rc, Rc is C1-4alkyl that is unsubstituted or substituted by hydroxy or halogen, (2) C1-4alkyl or C1-4alkoxyl that are unsubstituted or substituted by cyano or 1-3 halogens, or (3) 5-6-membered monocyclic heterocyclyl that is unsubstituted or substituted by cyano or trifluoromethyl.

[0035] In a further preferable embodiment of the present compound of general formula (I), R1 is phenyl, naphthyl or 5-6-membered saturated monocyclic heterocyclyl, all of which are unsubstituted or substituted by 1-3 R8, wherein R8 is
  1. (1) amino or -C(O)Rc, Rc is C1-4alkyl that is unsubstituted or substituted by hydroxy or halogen,
  2. (2) C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by cyano or 1-3 fluoro, or
  3. (3) piperazinyl or piperidinyl, both of which are unsubstituted or substituted by cyano or trifluoromethyl.


[0036] In a further preferable embodiment of the present compound of general formula (I), R1 is phenyl, piperidinyl or piperazinyl, all of which are unsubstituted or substituted by 1-2 R8, R8 is:
  1. (1) amino or -C(O)Rc, Rc is C1-2alkyl that is unsubstituted or substituted by hydroxy,
  2. (2) C1-2alkyl, which is unsubstituted or substituted by cyano or three fluoro, or
  3. (3) piperazinyl or piperidinyl, both of which are unsubstituted or substituted by cyano or trifluoromethyl.


[0037] In a preferable embodiment of the present compound of general formula (I), R2 is 3-14-membered cycloalkyl, 6-14-membered aryl or 3-14-membered heterocyclyl, all of which are unsubstituted or substituted by 1-3 R8', wherein R8' is selected from the group consisting of
  1. (1) hydroxy, halogen, amino, cyano, -(CH2)nNRaRb, -(CH2)nC(O)Rc, -(CH2)nS(O)mRc, -(CH2)nS(O)mNRaRb, -(CH2)nNRaS(O)mRc, -(CH2)nC(O)(CH2)nNRaRb, -(CH2)nOC(O)Rc, -(CH2)nNRaC(O)Rc, and -(CH2)nNRaC(O)NRaRb,
  2. (2) C1-6alkyl and C1-6alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from cyano, halogen and/or hydroxy, and
  3. (3) 3-8-membered monocyclic cycloalkyl, 6-14-membered aryl and 3-8-membered monocyclic heterocyclyl, which are unsubstituted or substituted by 1-3 substitutents selected from cyano, trifluoromethyl, halogen, C1-6alkyl, C1-6alkoxyl, -(CH2)nNRaRb, -(CH2)nC(O)Rc', -(CH2)nC(O)(CH2)nNRaRb, -(CH2)nS(O)mRc', -(CH2)nS(O)mNRaRb, -(CH2)nNRaS(O)mRc', -(CH2)nOC(O)Rc', -(CH2)nNRaC(O)Rc' and/or -(CH2)nNRaC(O)NRaRb;
wherein

each of Ra and Rb is independently selected from the group consisting of hydrogen, or C1-6alkyl that is unsubstituted or substituted by 1-3 substitutents selected from hydroxy, halogen and/or cyano;

Rc is C1-6alkyl or C1-6alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from hydroxy, halogen, cyano and/or trifluoromethyl; Rc' is C1-6alkyl or C1-6alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from hydroxy, halogen and/or cyano;

m is 0, 1 or 2; and

n is 0, 1, 2 or 3.



[0038] In a further preferable embodiment of the present compound of general formula (I), R2 is 6-14-membered aryl or 5-10-membered heterocyclyl, both of which are unsubstituted or substituted by 1-3 R8's, wherein R8' is selected from the group consisting of
  1. (1) hydroxy, halogen, cyano, amino, -(CH2)nC(O)Rc, -(CH2)nNRaRb, -(CH2)nS(O)mRc, -(CH2)nS(O)mNRaRb, -(CH2)nNRaS(O)mRc, -(CH2)nC(O)(CH2)nNRaRb, -(CH2)nOC(O)Rc, -(CH2)nNRaC(O)Rc and -(CH2)nNRaC(O)NRaRb,
  2. (2) C1-4alkyl and C1-4alkoxyl, which are unsubstituted or substituted by 1-3 substitutents selected from cyano, halogen and/or hydroxy, and
  3. (3) 5-8-membered monocyclic heterocyclyl, which is unsubstituted or substituted by 1-3 substitutents selected from cyano, trifluoromethyl, halogen, C1-4alkyl, C1-4alkoxyl, -(CH2)nNRaRb, -(CH2)nC(O)Rc', -(CH2)nC(O)(CH2)nNRaRb, -(CH2)nS(O)mRc', -(CH2)nS(O)mNRaRb, -(CH2)nNRaS(O)mRc', -(CH2)nOC(O)Rc', -(CH2)nNRaC(O)Rc' and/or -(CH2)nNRaC(O)NRaRb;

    each of Ra and Rb is independently hydrogen, or C1-4alkyl that is unsubstituted or substituted by 1-3 substitutents selected from hydroxy and/or halogen;

    Rc is C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from hydroxy, halogen and/or cyano;

    Rc' is C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from hydroxy and/or halogen;

    m is 0, 1 or 2; and

    n is 0, 1, 2 or 3.



[0039] In a further preferable embodiment of the present compound of general formula (I), R2 is 6-10-membered aryl, 5-6-membered partially saturated monocyclic heterocyclyl, 5-6-membered aromatic monocyclic heterocyclyl or 9-10-membered fused heterocyclyl, all of which are unsubstituted or substituted by 1-3 R8', wherein R8' is:
  1. (1) hydroxy, halogen, cyano, amino, -(CH2)nNRaC(O)Rc or -(CH2)nS(O)mRc, wherein Ra is hydrogen or C1-4alkyl that is unsubstituted or substituted by 1-3 substitutents selected from hydroxy and/or halogen, Rc is C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from hydroxy, halogen and/or cyano, m is 0, 1 or 2; and n is 0, 1, 2 or 3;
  2. (2) C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from cyano, halogen and/or hydroxy; or
  3. (3) 5-6-membered monocyclic heterocyclyl, which is unsubstituted or substituted by cyano, trifluoromethyl, halogen, C1-4alkyl or C1-4alkoxyl.


[0040] In a further preferable embodiment of the present compound of general formula (I), R2 is 6-10-membered aryl or 5-10-membered heterocyclyl, each of which are unsubstituted or substituted by 1-3 R8', wherein R8' is (1) hydroxy, halogen, amino, cyano, -NHC(O)Rc or -S(O)mRc, wherein m is 0, 1 or 2, Rc is C1-4alkyl, (2) C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by hydroxy or 1-3 halogens, or (3) 5-6-membered monocyclic heterocyclyl, which is unsubstituted or substituted by cyano, trifluoromethyl, halogen, C1-4alkyl or C1-4alkoxyl.

[0041] In a further preferable embodiment of the present compound of general formula (I), R2 is 6-10-membered aryl, 5-6-membered partially saturated monocyclic heterocyclyl, 5-6-membered aromatic monocyclic heterocyclyl or 9-10-membered fused heterocyclyl, all of which are unsubstituted or substituted by 1-3 R8', wherein R8' is (1) hydroxy, halogen, amino, cyano, -NHC(O)Rc or -S(O)mRc, wherein m is 0, 1 or 2, Rc is C1-4alkyl, (2) C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by hydroxy or 1-3 halogens, or (3) 5-6-membered monocyclic heterocyclyl, which is unsubstituted or substituted by cyano, trifluoromethyl, halogen, C1-4alkyl or C1-4alkoxyl.

[0042] In a further preferable embodiment of the present compound of general formula (I), R2 is phenyl, pyridinyl, pyrimidinyl, pyridazinyl, pyrazinyl, pyrazolyl, imidazolyl, pyrrolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, indazolyl, quinolinyl, isoquinolinyl, indolyl, pyrrolopyridine, dihydropyrrolopyridine or pyrazolopyridinyl, all of which are unsubstituted or substituted by 1-3 R8', wherein R8' is:
  1. (1) hydroxy, halogen, amino, cyano, -NHC(O)Rc or -S(O)mRc, wherein m is 0 or 2, Rc is C1-4alkyl,
  2. (2) C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by hydroxy or 1-3 fluoro, or
  3. (3) pyridinyl, piperidinyl, pyrimidinyl, pyridazinyl, pyrazinyl, piperazinyl, pyrazolyl, imidazolyl, pyrrolyl, pyrrolidinyl or morpholinyl, all of which are unsubstituted or substituted by cyano, trifluoromethyl, halogen, C1-4alkyl or C1-4alkoxyl.


[0043] In a further preferable embodiment of the present compound of general formula (I), R2 is phenyl, pyridinyl, pyrimidinyl, pyridazinyl, pyrazinyl, pyrazolyl, imidazolyl, pyrrolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, quinolinyl, isoquinolinyl, pyrrolopyridine, dihydropyrrolopyridine or indolyl, all of which are unsubstituted or substituted by 1-2 R8', wherein R8' is:
  1. (1) halogen, amino, cyano, -NHC(O)Rc or -S(O)mRc, wherein m is 0 or 2, Rc is C1-4alkyl,
  2. (2) C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by hydroxy or three fluoro, or
  3. (3) piperazinyl, pyrazolyl, piperidinyl, morpholinyl, pyrimidinyl, pyridazinyl, pyrazinyl, pyrrolyl or pyrrolidinyl, all of which are unsubstituted or substituted by C1-4alkyl.


[0044] In a preferable embodiment of the present compound of general formula (I), each of R3 and R4 is independently selected from the group consisting of hydrogen, halogen, cyano, amino, hydroxy, and C1-6alkyl and C1-6alkoxyl that are unsubstituted or substituted by hydroxy or 1-3 halogens.

[0045] In a further preferable embodiment of the present compound of general formula (I), each of R3 and R4 are independently selected from the group consisting of hydrogen, halogen, cyano, amino, hydroxy, trifluoromethyl and trifluoromethoxy. In a further preferable embodiment of the present compound of general formula (I), each of R3 and R4 is hydrogen.

[0046] In a preferable embodiment of the present compound of general formula (I), R5 is hydrogen, cyano, amino, or C1-6alkyl or C1-6alkoxyl, each of which are unsubstituted or substituted by hydroxy, carboxyl or 1-3 halogens.

[0047] In a further preferable embodiment of the present compound of general formula (I), R5 is hydrogen.

[0048] In a preferable embodiment of the present compound of general formula (I), each of R6 and R7 is independently selected from the group consisting of hydrogen, hydroxy, halogen, amino, and C1-6alkyl and C1-6alkoxyl that are unsubstituted or substituted by hydroxy or 1-3 halogens.

[0049] In a further preferable embodiment of the present compound of general formula (I), each of R6 and R7 is independently hydrogen or C1-4alkyl.

[0050] In another preferable embodiment of the present compound of general formula (I),

R1 is 3-14-membered cycloalkyl, 6-14-membered aryl, 3-14-membered heterocyclyl, 7-12-membered spiro ring group or 7-12-membered bridged ring group, all of which are unsubstituted or substituted by 1-3 R8;

R2 is 3-14-membered cycloalkyl, 6-14-membered aryl or 3-14-membered heterocyclyl, all of which are unsubstituted or substituted by 1-3 R8';

each of R3 and R4 is independently selected from the group consisting of hydrogen, halogen, cyano, amino, hydroxy, trifluoromethyl and trifluoromethoxy;

R5 is hydrogen, cyano or amino;

each of R6 and R7 is independently selected from the group consisting of hydrogen, hydroxy, halogen, amino, and C1-6alkyl;

each of R8 and R8' is independently selected from the group consisting of

  1. (1) hydroxy, halogen, amino, cyano, -(CH2)nC(O)Rc, -(CH2)nNRaRb, -(CH2)nS(O)mRc, -(CH2)nS(O)mNRaRb, -(CH2)nNRaS(O)mRc, -(CH2)nC(O)(CH2)nNRaRb, -(CH2)nOC(O)Rc, -(CH2)nNRaC(O)Rc and -(CH2)nNRaC(O)NRaRb,
  2. (2) C1-6alkyl and C1-6alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from cyano, halogen and/or hydroxy, and
  3. (3) 3-8-membered monocyclic cycloalkyl, 6-14-membered aryl and 3-8-membered monocyclic heterocyclyl, which are unsubstituted or substituted by 1-3 substitutents selected from cyano, trifluoromethyl, halogen, C1-6alkyl, C1-6alkoxyl, -(CH2)nNRaRb, -(CH2)nC(O)Rc', -(CH2)nC(O)(CH2)nNRaRb, -(CH2)nS(O)mRc', -(CH2)nS(O)mNRaRb, -(CH2)nNRaS(O)mRc', -(CH2)nOC(O)Rc', -(CH2)nNRaC(O)Rc' and/or -(CH2)nNRaC(O)NRaRb;
wherein

each of Ra and Rb is independently selected from the group consisting of hydrogen, or C1-6alkyl that is unsubstituted or substituted by 1-3 substitutents selected from hydroxy, halogen and/or cyano;

Rc is C1-6alkyl and C1-6alkoxyl, each of which are unsubstituted or substituted by 1-3 substitutents selected from hydroxy, halogen, cyano and/or trifluoromethyl; Rc' is C1-6alkyl or C1-6alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from hydroxy, halogen and/or cyano;

m is 0, 1 or 2; and

n is 0, 1, 2 or 3.



[0051] In another preferable embodiment of the present compound of general formula (I),

R1 is 3-8-membered monocyclic cycloalkyl, 6-14-membered aryl or 3-8-membered monocyclic heterocyclyl, each of which are unsubstituted or substituted by 1-3 R8;

R2 is 3-8-membered monocyclic cycloalkyl, 6-14-membered aryl or 5-10-membered heterocyclyl, all of which are unsubstituted or substituted by 1-3 R8';

each of R3, R4 and R5 is hydrogen;

each of R6 and R7 is independently hydrogen or C1-4alkyl;

R8 is selected from the group consisting of

  1. (1) hydroxy, halogen, cyano, amino, and -(CH2)nC(O)Rc,
  2. (2) C1-4alkyl and C1-4alkoxyl, which are unsubstituted or substituted by 1-3 substitutents selected from cyano, halogen and/or hydroxy, and
  3. (3) 5-8-membered saturated monocyclic heterocyclyl, which is unsubstituted or substituted by 1-3 substitutents selected from cyano, trifluoromethyl, halogen, -(CH2)nC(O)Rc', -(CH2)nC(O)(CH2)nNRaRb and/or -(CH2)nS(O)mRc';

R8' is selected from the group consisting of

  1. (1) hydroxy, halogen, cyano, amino, -(CH2)nC(O)Rc, -(CH2)nNRaRb, -(CH2)nS(O)mRc, -(CH2)nS(O)mNRaRb, -(CH2)nNRaS(O)mRc, -(CH2)nC(O)(CH2)nNRaRb, -(CH2)nOC(O)Rc, -(CH2)nNRaC(O)Rc and -(CH2)nNRaC(O)NRaRb,
  2. (2) C1-4alkyl and C1-4alkoxyl, which are unsubstituted or substituted by 1-3 substitutents selected from cyano, halogen and/or hydroxy, and
  3. (3) 5-8-membered monocyclic heterocyclyl, which is unsubstituted or substituted by 1-3 substitutents selected from cyano, trifluoromethyl, halogen, C1-4alkyl, C1-4alkoxyl, -(CH2)nNRaRb, -(CH2)nC(O)Rc', -(CH2)nC(O)(CH2)nNRaRb, -(CH2)nS(O)mRc', -(CH2)nS(O)mNRaRb, -(CH2)nNRaS(O)mRc', -(CH2)nOC(O)Rc', -(CH2)nNRaC(O)Rc' and/or -(CH2)nNRaC(O)NRaRb;

each of Ra and Rb is independently hydrogen, or C1-4alkyl that is unsubstituted or substituted by 1-3 substitutents selected from hydroxy and/or halogen;

Rc is C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from hydroxy, halogen and/or cyano;

Rc' is C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from hydroxy and/or halogen;

m is 0, 1 or 2; and

n is 0, 1, 2 or 3.



[0052] In a further preferable embodiment of the present compound of general formula (I):

R1 is 6-10-membered aryl, 3-8-membered saturated monocyclic heterocyclyl or 5-6-membered aromatic monocyclic heterocyclyl, all of which are unsubstituted or substituted by 1-3 R8;

R2 is 6-10-membered aryl, 5-6-membered partially saturated monocyclic heterocyclyl, 5-6-membered aromatic monocyclic heterocyclyl or

9-10-membered fused heterocyclyl, all of which are unsubstituted or substituted by 1-3 R8';

each of R3, R4 and R5 is hydrogen;

each of R6 and R7 is independently hydrogen or C1-4alkyl;

R8 is

  1. (1) hydroxy, halogen, cyano, amino or -C(O)Rc,
  2. (2) C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from cyano, halogen and/or hydroxy, or
  3. (3) 5-6-membered saturated monocyclic heterocyclyl, which is unsubstituted or substituted by 1-3 substitutents selected from cyano, trifluoromethyl, halogen, -C(O)Rc', -C(O)(CH2)nNRaRb and/or -S(O)2Rc';

R8' is

  1. (1) hydroxy, halogen, cyano, amino, -(CH2)nNRaC(O)Rc or -(CH2)nS(O)mRc,
  2. (2) C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from cyano, halogen and/or hydroxy, or
  3. (3) 5-6-membered monocyclic heterocyclyl, which is unsubstituted or substituted by cyano, trifluoromethyl, halogen, C1-4alkyl or C1-4alkoxyl;

each of Ra and Rb is independently hydrogen, or C1-4alkyl that is unsubstituted or substituted by 1-3 substitutents selected from hydroxy and/or halogen;

Rc is C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from hydroxy, halogen and/or cyano;

Rc' is C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from hydroxy and/or halogen;

m is 0, 1 or 2; and

n is 0, 1, 2 or 3.



[0053] In a further preferable embodiment of the present compound of general formula (I):

R1 is phenyl, naphthyl or 5-8-membered saturated monocyclic heterocyclyl that are unsubstituted or substituted by 1-3 R8;

R2 is phenyl, pyridinyl, pyrimidinyl, pyridazinyl, pyrazinyl, pyrazolyl, imidazolyl, pyrrolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, indazolyl, quinolinyl, isoquinolinyl, indolyl, pyrrolopyridine, dihydropyrrolopyridine or pyrazolopyridinyl, all of which are unsubstituted or substituted by 1-3 R8;

each of R3, R4 and R5 is hydrogen;

each of R6 and R7 is independently hydrogen or methyl;

R8 is

  1. (1) amino or -C(O)Rc, Rc is C1-4alkyl that is unsubstituted or substituted by hydroxy or halogen,
  2. (2) C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by cyano or 1-3 fluoro, or
  3. (3) piperazinyl or piperidinyl, both of which are unsubstituted or substituted by cyano or trifluoromethyl;

R8' is

  1. (1) hydroxy, halogen, amino, cyano, -NHC(O)Rc or -S(O)mRc, wherein m is 0 or 2, Rc is C1-4alkyl,
  2. (2) C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by hydroxy or 1-3 fluoro, or
  3. (3) pyridinyl, piperidinyl, pyrimidinyl, pyridazinyl, pyrazinyl, piperazinyl, pyrazolyl, imidazolyl, pyrrolyl, pyrrolidinyl or morpholinyl, all of which are unsubstituted or substituted by cyano, trifluoromethyl, halogen, C1-4alkyl or C1-4alkoxyl.



[0054] In a further preferable embodiment of the present compound of general formula (I),

R1 is phenyl, piperidinyl or piperazinyl, all of which are unsubstituted or substituted by 1-2 R8;

R2 is phenyl, pyridinyl, pyrimidinyl, pyridazinyl, pyrazinyl, pyrazolyl, imidazolyl, pyrrolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, quinolinyl, isoquinolinyl, pyrrolopyridine, dihydropyrrolopyridine or indolyl, all of which are unsubstituted or substituted by 1-2 R8';

each of R3, R4 and R5 is hydrogen;

each of R6 and R7 is independently hydrogen or methyl;

R8 is

  1. (1) amino or -C(O)Rc, Rc is C1-4alkyl that is unsubstituted or substituted by hydroxy,
  2. (2) C1-4alkyl, which is unsubstituted or substituted by cyano or three fluoro, or
  3. (3) piperazinyl or piperidinyl, both of which are unsubstituted or substituted by cyano or trifluoromethyl;

R8' is

  1. (1) halogen, amino, cyano, -NHC(O)Rc or -S(O)mRc, wherein m is 0 or 2, Rc is C1-4alkyl,
  2. (2) C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by hydroxy or three fluoro, or
  3. (3) piperazinyl, pyrazolyl, piperidinyl, morpholinyl, pyrimidinyl, pyridazinyl, pyrazinyl, pyrrolyl or pyrrolidinyl, all of which are unsubstituted or substituted by C1-4alkyl.



[0055] The preferable compounds of the present invention comprise the following compounds and its pharmaceutically acceptable salt, stereoisomer and deuteride thereof:
No. Structure Name
1

2-(6-aminopyridin-3-yl)-10-(3-(trifluoromethyl)p henyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one
2

2-(6-methoxypyridin-3-yl)-10-(4-(piperazin-1-yl) -3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]nap hthyridin-9(10H)-one
3

(R)-2-(6-aminopyridin-3-yl)-10-(1-(2-hydroxypro panoyl)piperidin-4-yl)pyrido [3,2-c][1,5]naphthyri din-9(10H)-one
4

(R)-10-(1-(2-hydroxypropanoyl)piperidin-4-yl)-2-(6-methoxypyridin-3-yl)pyrido[3,2-c][1,5]naphth yridin-9(10H)-one
5

2-methyl-2-(4-(9-oxo-2-(quinolin-3-yl)pyrido[3,2 -c][1,5]naphthyridin-10(9H)-yl)phenyl)propaneni trile
6

2-(6-aminopyridin-3-yl)-10-(4-(piperazin-1-yl)-3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphth yridin-9(10H)-one
7

2-(4-(2-(6-aminopyridin-3-yl)-9-oxopyrido[3,2-c] [1,5]naphthyridin-10(9H)-yl)phenyl)-2-methylpro panenitrile
8

2-(6-methoxypyridin-3-yl)-10-(3-(trifluoromethyl )phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne
9

2-(quinolin-3-yl)-10-(3-(trifluoromethyl)phenyl)p yrido[3,2-c][1,5]naphthyridin-9(10H)-one
10

10-(4-(piperazin-1-yl)-3-(trifluoromethyl)phenyl) -2-(quinolin-3-yl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one
11

(R)-10-(1-(2-hydroxypropanoyl)piperidin-4-yl)-2-(quinolin-3-yl)pyrido[3,2-c][1,5]naphthyridin-9(1 0H)-one
12

2-(4-(2-(6-methoxypyridin-3-yl)-9-oxopyrido[3,2 -c][1,5]naphthyridin-10(9H)-yl)phenyl)-2-methyl propanenitrile
13

2-(2-aminopyrimidin-5-yl)-10-(3-(trifluoromethyl )phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne
14

2-(2-methoxypyrimidin-5-yl)-10-(3-(trifluoromet hyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H )-one
15

N-(5-(9-oxo-10-(3-(trifluoromethyl)phenyl)-9,10-dihydropyrido [3,2-c][1,5]naphthyridin-2-yl)pyrid in-2-yl)acetamide
16

5-(9-oxo-10-(3-(trifluoromethyl)phenyl)-9,10-dih ydropyrido[3,2-c][1,5]naphthyridin-2-yl)-2-cyano pyridine
17

2-(1H-pyrrolo[2,3-b]pyridin-3-yl)-10-(3-(trifluor omethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9 (10H)-one
18

2-(6-(hydroxymethyl)pyridin-3-yl)-10-(3-(trifluor omethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9 (10H)-one
19

2-(6-(4-methylpiperazin-1-yl)pyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphth yridin-9(10H)-one
20

2-(6-(1H-pyrazol-1-yl)pyridin-3-yl)-10-(3-(trifluo romethyl)phenyl)pyrido [3,2-c][1,5]naphthyridin-9(10H)-one
21

2-(4-(2-(2-methoxypyrimidin-5-yl)-9-oxopyrido[ 3,2-c][1,5]naphthyridin-10(9H)-yl)phenyl)-2-met hylpropanenitrile
22

2-(6-(methylsulfonyl)pyridin-3-yl)-10-(3-(trifluor omethyl)phenyl)pyrido [3,2-c][1,5]naphthyridin-9 (10H)-one
23

2-(6-methoxypyridin-3-yl)-10-(3-(trifluoromethyl )phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne
24

2-(6-morpholinopyridin-3-yl)-10-(3-(trifluoromet hyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H )-one
25

2-(5-methoxypyridin-3-yl)-10-(3-(trifluoromethyl )phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne
26

2-(2-methoxypyridin-3-yl)-10-(3-(trifluoromethyl )phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne
27

10-(3-(trifluoromethyl)phenyl)-2-(6-(trifluoromet hyl)pyridin-3-yl)pyrido[3,2-c][1,5]naphthyridin-9 (10H)-one
28

2-(6-methylpyridin-3-yl)-10-(3-(trifluoromethyl)p henyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one
29

2-(3,5-dimethylisoxazol-4-yl)-10-(3-(trifluoromet hyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H )-one
30

2-(5-fluoropyridin-3-yl)-10-(3-(trifluoromethyl)p henyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one
31

10-(3-(trifluoromethyl)phenyl-2-(5-(trifluorometh yl)pyridin-3-yl)pyrido[3,2-c][1,5]naphthyridin-9( 10H)-one
32

2-(1-methyl-1H-pyrazol-5-yl)-10-(3-(trifluoromet hyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H )-one
33

2-(thiazol-5-yl)-10-(3-(trifluoromethyl)phenyl)py rido[3,2-c][1,5]naphthyridin-9(10H)-one
34

3-methyl-5-(9-oxo-10-(3-(trifluoromethyl)phenyl) -9,10-dihydropyrido[3,2-c][1,5]naphthyridin-2-yl )-2-cyanopyridine
35

5-(9-oxo-10-(3-(trifluoromethyl)phenyl)-9,10-dih ydropyrido[3,2-c][1,5]naphthyridin-2-yl)-3-cyano pyridine
36

2-(6-(pyrrolidin-1-yl)pyridin-3-yl)-10-(3-(trifluor omethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9 (10H)-one
37

2-(1-methyl-1H-pyrazol-4-yl)-10-(3-(trifluoromet hyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H )-one
38

2-(6-(methylthio)pyridin-3-yl)-10-(3-(trifluorome thyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10 H)-one
39

2-(1H-pyrrolo[2,3-b]pyridin-5-yl)-10-(3-(trifluor omethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9 (10H)-one
40

2-(6-(methylsulfonyl)pyridin-3-yl)-10-(4-(piperaz in-1-yl)-3-(trifluoromethyl)phenyl)pyrido[3,2-c][ 1,5]naphthyridin-9(10H)-one
41

(R)-10-(1-(2-hydroxypropanoyl)piperidin-4-yl)-2-(6-(methylsulfonyl)pyridin-3-yl)pyrido[3,2-c][1,5 ]naphthyridin-9(10H)-one
42

2-methyl-2-(4-(2-(6-(methylsulfonyl)pyridin-3-yl )-9-oxopyrido[3,2-c][1,5]naphthyridin-10(9H)-yl) phenyl)propanenitrile
43

N-(5-(9-oxo-10-(4-(piperazin-1-yl)-3-(trifluorom ethyl)phenyl)-9,10-dihydropyrido[3,2-c][1,5]naph thyridin-2-yl)pyridin-2-yl)acetamide
44

(R)-N-(5-(10-(1-(2-hydroxypropanoyl)piperidin-4 -yl)-9-oxo-9,10-dihydropyrido[3,2-c][1,5]naphthy ridin-2-yl)pyridin-2-yl)acetamide
45

N-(5-(10-(4-(2-cyanopropan-2-yl)phenyl)-9-oxo-9,10-dihydropyrido[3,2-c][1,5]naphthyridin-2-yl) pyridin-2-yl)acetamide
46

5-(9-oxo-10-(4-(piperazin-1-yl)-3-(trifluoromethy l)phenyl)-9,10-dihydropyrido[3,2-c][1,5]naphthyr idin-2-yl)-2-cyanopyridine
47

(R)-(5-(10-(1-(2-hydroxypropanoyl)piperidin-4-yl )-9-oxo-9,10-dihydropyrido[3,2-c][1,5]naphthyrid in-2-yl)-2-cyanopyridine
48

5-(10-(4-(2-cyanopropan-2-yl)phenyl)-9-oxo-9,10 -dihydropyrido[3,2-c][1,5]naphthyridin-2-yl)-2-c yanopyridine
49

2-(6-(methylthio)pyridin-3-yl)-10-(4-(piperazin-1 -yl)-3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5] naphthyridin-9(10H)-one
50

(R)-10-(1-(2-hydroxypropanoyl)piperidin-4-yl)-2-(6-(methylthio)pyridin-3-yl)pyrido[3,2-c][1,5]nap hthyridin-9(10H)-one
51

2-methyl-2-(4-(2-(6-(methylthio)pyridin-3-yl)-9-oxopyrido[3,2-c][1,5]naphthyridin-10(9H)-yl)phe nyl)propanenitrile


[0056] In the present invention, the term "halogen" means fluoro, chloro, bromo or iodo. In the present invention, the term "C1-6alkyl" means a straight-chain or branched chain alkyl containing 1-6 carbon atoms, wherein including, for example "C1-4alkyl", "C2-5alkyl", "C1-3alkyl" and the like; its example includes but is not limited to, for example, methyl, ethyl, n-propyl, iso-propyl, n-butyl, 2-methylpropyl, 1-methylpropyl, 1,1-dimethylethyl, n-pentyl, 3-methylbutyl, 2-methylbutyl, 1-methylbutyl, 1-ethylpropyl, n-hexyl, 4-methylpentyl, 3-methylpentyl, 2-methylpentyl, 1-methylpentyl, 3,3-dimethylbutyl, 2,2-dimethylbutyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 1,3-dimethylbutyl, 2,3-dimethylbutyl, 2-ethylbutyl, 1,2-dimethylpropyl and the like.

[0057] In the present invention, the term "C2-6alkenyl" means a straight-chain, brached chain or cyclic hydrocarbonyl containing 2-6 carbon atoms and double bond(s), wherein including for example "C2-4alkenyl", "C2-5alkenyl", "C2-3alkenyl", C3-6cycloalkenyl and the like; its example includes but is not limited to, for example, ethenyl, 1-propenyl, 2-propenyl, 1-methylethenyl, 1-butenyl, 2-butenyl, 3-butenyl, 1-methyl-1-propenyl, 2-methyl-1-propenyl, 1-methyl-2-propenyl, 2-methyl-2-propenyl, 1-pentenyl, 2-pentenyl, 3-pentenyl, 4-pentenyl, 1-methyl-1-butenyl, 2-methyl-1-butenyl, 3-methyl-1-butenyl, 1-methyl-2-butenyl, 2-methyl-2-butenyl, 3-methyl-2-butenyl, 1-methyl-3-butenyl, 2-methyl-3-butenyl, 3-methyl-3-butenyl, 1,1-dimethyl-2-propenyl, 1,2-dimethyl-1-propenyl, 1,2-dimethyl-2-propenyl, 1-ethyl-1-propenyl, 1-ethyl-2-propenyl, 1-hexenyl, 2-hexenyl, 3-hexenyl, 4-hexenyl, 5-hexenyl, 1-methyl-1-pentenyl, 2-methyl-1-pentenyl, 3-methyl-1-pentenyl, 4-methyl-1-pentenyl, 1-methyl-2-pentenyl, 2-methyl-2-pentenyl, 3-methyl-2-pentenyl, 4-methyl-2-pentenyl, 1-methyl-3-pentenyl, 2-methyl-3-pentenyl, 3-methyl-3-pentenyl, 4-methyl-3-pentenyl, 1-methyl-4-pentenyl, 2-methyl-4-pentenyl, 3-methyl-4-pentenyl, 4-methyl-4-pentenyl, 1,1-dimethyl-2-butenyl, 1,1-dimethyl-3-butenyl, 1,2-dimethyl-1-butenyl, 1,2-dimethyl-2-butenyl, 1,2-dimethyl-3-butenyl, 1,3-dimethyl-1-butenyl, 1,3-dimethyl-2-butenyl, 1,3-dimethyl-2-butenyl, 2,2-dimethyl-3-butenyl, 2,3-dimethyl-1-butenyl, 2,3-dimethyl-2-butenyl, 2,3-dimethyl-3-butenyl, 3,3-dimethyl-1-butenyl, 3,3-dimethyl-2-butenyl, 1-ethyl-1-butenyl, 1-ethyl-2-butenyl, 1-ethyl-3-butenyl, 2-ethyl-1-butenyl, 2-ethyl-2-butenyl, 2-ethyl-3-butenyl, 1,1,2-trimethyl-2-propenyl, 1-ethyl-1-methyl-2-propenyl, 1-ethyl-2-methyl-1-propenyl, 1-ethyl-2-methyl-2-propenyl, 1,3-butadiene, 1,3-pentadiene, 1,4-pentadiene, 1,4-hexadiene and the like. The example of said "C3-6cycloalkenyl" includes but is not limited to, for example, cyclopropenyl, cyclobutenyl, cyclopentenyl, 1,3-cyclopentadienyl, cyclohexenyl, 1,4-cyclohexadienyl and the like.

[0058] In the present invention, the term "C2-6alkynyl" means a straight-chain, or brached chain hydrocarbonyl containing 2-6 carbon atoms and triple bond(s), wherein including for example "C2-4alkynyl", "C2-5alkynyl", "C2-3alkynyl" the like; its example includes but is not limited to, for example, ethynyl, 2-propynyl, 2-butynyl, 3-butynyl, 1-methyl-2-propynyl, 2-pentynyl, 3-pentynyl, 4-pentynyl, 1-methyl-2-butynyl, 1-methyl-3-butynyl, 2-methyl-3-butynyl, 1,1-dimethyl-2-propynyl, 1-ethyl-2-propynyl, 2-hexynyl, 3-hexynyl, 4-hexynyl, 5-hexynyl, 1-methyl-2-pentynyl, 1-methyl-3-pentynyl, 1-methyl-4-pentynyl, 2-methyl-3-pentynyl, 2-methyl-4-pentynyl, 3-methyl-4-pentynyl, 4-methyl-2-pentynyl, 1,1-dimethyl-2-butynyl, 1,1-dimethyl-3-butynyl, 1,2-dimethyl-3-butynyl, 2,2-dimethyl-3-butynyl, 1-ethyl-2-butynyl, 1-ethyl-3-butynyl, 2-ethyl-3-butynyl, 1-ethyl-1-methyl-2-propynyl and the like. In the present invention, the term "C1-6alkoxy" means a "C1-6alkyl-O-" group, wherein C1-6alkyl is defined as hereinbefore, wherein including for example "C1-4alkoxyl", "C2-5alkoxyl", "C1-3alkoxyl" and the like; its example includes but is not limited to, for example, methoxy, ethoxy, propoxy, isopropoxy, butoxy, isobutoxy, tert-butoxy, sec-butoxy, pentyloxy, neo-pentyloxy, hexyloxy, and the like.

[0059] In the present invention, the term "3-14-membered cycloalkyl" means a cycloalkyl having 3-14 carbon atoms, wherein including for example "3-12-membered cycloalkyl", "5-10-membered cycloalkyl", "3-8-membered cycloalkyl", "3-6-membered cycloalkyl", "5-8-membered cycloalkyl" and the like; also including "3-8-membered monocyclic cycloalkyl" and "6-14-membered fused cycloalkyl".

[0060] Said "3-8-membered monocyclic cycloalkyl" means monocycloalkyl having 3-8 carbon atoms, wherein including for example "3-6-membered monocyclic cycloalkyl", "5-8-membered monocyclic cycloalkyl", "5-6-membered monocyclic cycloalkyl" and like; its example includes but is not limited to: cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctanyl and the like; said 3-8-membered monocyclic cycloalkyl can also be further substutited by C1-6alkyl, including but being not limited to, methylcyclopropyl, dimethylcyclopropyl, methylcyclobutyl, dimethylcyclobutyl, methylcyclopentyl, dimethylcyclopentyl, methylcyclohexyl, dimethylcyclohexanyl and the like.

[0061] Said "6-14-membered fused cycloalkyl" means a fused cycloalkyl group, which is formed by two or more cyclic structures in said fused ring via sharing two adjacent carbon atoms with each other, wherein including for example "6-12-membered fused cycloalkyl", "8-12-membered fused cycloalkyl", "7-10-membered fused cycloalkyl" and the like; its example includes but is not limited to: dicyclo[3.1.0]hexyl, dicyclo[4.1.0]heptyl, dicyclo[2.2.0]hexyl, dicyclo[3.2.0]heptyl, dicyclo[4.2.0]octyl, octahydro-1H-indenyl, decahydronaphthalenyl, tetradecahydrophenanthrenyl and the like.

[0062] In the present invention, the term "6-14-membered aryl" means an aromatic group having 6-14 carbon atoms, wherein including for example "6-10-membered aryl" and the like; also including "6-8-membered monocyclic aryl" and "8-14-membered fused aryl".

[0063] Said "6-8-membered monocyclic aryl" includes, for example, phenyl, cyclooctatetraeneyl and the like.

[0064] Said "8-14-membered fused aryl" means a fused-ring group, which has 8-14 carbon atoms and is formed by two or more cyclic structures via sharing two adjacent carbon atoms with each other, and in which at least one ring is aromatic, wherein including 10-14-membered fused aryl wherein all of the rings are aromatic rings, for example naphthyl, phenanthrenyl and the like, also including 8-14-membered fused aryl wherein a part of the rings are aromatic rings, for example benzene-fused 3-8-membered monocyclic cycloalkyl, benzene-fused C3-6cycloalkenyl and the like. Its specific example includes but is not limited to, for example, 2,3-dihydro-1H-indenyl, 1H-indenyl, 1,2,3,4-tetrahydronaphthyl, 1,4-dihydronaphthyl and the like.

[0065] In the present invention, the term "7-12-membered bridged ring group" means an aliphatic cyclic hydrocarbyl containing 7-12 ring atoms, which is formed via any two rings sharing two indirectly attached atoms, wherein all of said ring atoms can be carbon atoms, or said ring atoms can contain at least one heteroatom selected from N, O and S and the like. Said "7-12-membered bridged ring group" includes "7-12-membered saturated bridged group" and "7-12-membered non-saturated bridged ring group".

[0066] Said "7-12-membered saturated bridged ring group" means all of the rings in the bridged ring group are saturated cyclic groups, wherein including for example "7-10-membered saturated bridged ring group", "7-8-membered saturated bridged ring group" and the like; its specific example includes but is not limited to, for example, the following bridged ring groups:







and the like; preferably 7-8-membered saturated bridged ring group.

[0067] Said "7-12-membered non-saturated bridged ring group" means a cyclic group, in which at least one ring is non-saturated, wherein including for example "7-10-membered non-saturated bridged ring group", "7-8-membered non-saturated bridged ring group" and the like; its specific example includes but is not limited to, for example, the following bridged ring groups:



and the like; preferably 7-8-membered non-saturated bridged ring group.

[0068] In the present invention, the term "7-12-membered spiro ring group" means an aliphatic cyclic hydrocarbyl that is formed via at least two rings sharing one atom and has 7-12 ring atoms; All of the ring atoms can be carbon atoms, or the ring atom contains at least one heteroatom selected from N, O, S and the like; wherein including for example "7-11-membered spiro ring group", "8-11-membered spiro ring group", "9-10-membered spiro ring group" and the like, also including "7-12-membered saturated spiro ring group" and "7-12-membered non-saturated spiro ring group".

[0069] Said "7-12-membered saturated spiro ring group" means all of the rings in said spiro ring group are saturated rings; wherein including for example "7-11-membered saturated spiro ring group", "8-11-membered saturated spiro ring group", "9-10-membered saturated spiro ring group" and the like; its specific example includes but is not limited to, for example, the following spiro ring groups:







and the like.

[0070] Said "7-12-membered non-saturated spiro ring group" means at least one ring in said spiro ring group is/are non-saturated ring(s), wherein including for example "7-11-membered non-saturated spiro ring group", "8-11-membered non-saturated spiro ring group", "9-10-membered non-saturated spiro ring group" and the like; its specific example includes but is not limited to, for example, the following spiro ring groups:





and the like.

[0071] In the present invention, the term "3-14-membered heterocyclyl" means a cyclic group containing 3-14 ring atoms (wherein containing at least one hetero atom), wherein including for example "3-8-membered heterocyclyl", "4-12-membered heterocyclyl", "5-10-membered heterocyclyl", "5-8-membered heterocyclyl", "5-6-membered heterocyclyl", and the like, also including "3-8-membered monocyclic heterocyclyl" and "6-14-membered fused heterocyclyl", wherein said hetero atom is selected from N, O, S and the like.

[0072] Said "3-8-membered monocyclic heterocyclyl" means a monocyclic heterocyclyl containing 3-8 ring atoms (wherein containing at least one hetero atom), wherein including for example "5-8-membered monocyclic heterocyclyl", "5-6-membered monocyclic heterocyclyl" and the like, also including "5-8-membered aromatic monocyclic heterocyclyl", "3-8-membered partially saturated monocyclic heterocyclyl" and "3-8-membered saturated monocyclic heterocyclyl".

[0073] Said "5-8-membered aromatic monocyclic heterocyclyl" means an aromatic cyclic group containing at least one hetero atom, wherein including for example "5-6-membered aromatic monocyclic heterocyclyl", "5-7-membered aromatic monocyclic heterocyclyl" and the like; its specific example includes but is not limited to, for example, furyl, thienyl, pyrrolyl, thiazolyl, thiodiazolyl, oxazolyl, oxdiazolyl, imidazolyl, pyrazolyl, pyridinyl, pyrimidinyl, 1,4-dioxinyl, 2H-1,2-oxazinyl, 4H-1,2-oxazinyl, 6H-1,2-oxazinyl, 4H-1,3-oxazinyl, 6H-1,3-oxazinyl, 4H-1,4-oxazinyl, pyridazinyl, pyrazinyl, 1,2,3-triazinyl, 1,2,4-triazinyl, 1,3,5-triazinyl, 1,2,4,5-tetrazinyl, oxepinyl, thiepinyl, azepinyl, 1,3-diazepinyl, azocinyl and the like.

[0074] Said "3-8-membered partially saturated monocyclic heterocyclyl" means a monocyclic heterocyclyl containing a double bond, wherein including for example "5-8-membered partially saturated monocyclic heterocyclyl", "5-6-membered partially saturated monocyclic heterocyclyl" and the like; its specific example includes but is not limited to, for example, 2,5-dihydrothienyl, 4,5-dihydropyrazolyl, 3,4-dihydro-2H-pyranyl, 5,6-dihydro-4H-1,3-oxazinyl and the like.

[0075] Said "3-8-membered saturated monocyclic heterocyclyl" means a monocyclic heterocyclyl, in which all of bonds are saturated, wherein including for example "5-8-membered saturated monocyclic heterocyclyl", "5-6-membered saturated monocyclic heterocyclyl", "3-6-membered saturated monocyclic heterocyclyl" and the like; its specific example includes but is not limited to, for example, aziridinyl, azetidinyl, thietanyl, tetrahydrofuryl, tetrahydropyrrolyl, imidazolidinyl, pyrazolidinyl, tetrahydrofuryl, 1,4-dioxanyl, 1,3-dioxanyl, 1,3-dithianyl, morpholinyl, piperazinyl and the like.

[0076] Said "6-14-membered fused heterocyclyl" means a fused heterocyclyl, which contains 6-14 ring atoms (wherein containing at least one hetero atom), and which is formed by linking via two or more cyclic structures sharing two adjacent atoms with each other, wherein including for example "8-12-membered fused heterocyclyl", "7-10-membered fused heterocyclyl", "9-10-membered fused heterocyclyl", "9-12-membered fused heterocyclyl" and the like, also including "8-14-membered aromatic fused heterocyclyl", "6-14-membered partially saturated fused heterocyclyl" and "6-14-membered saturated fused heterocyclyl". Said "8-14-membered aromatic fused heterocyclyl" means a fused heterocyclyl, in which all of rings are aromatic rings, wherein including for example "8-12-membered aromatic fused heterocyclyl", "9-10-membered aromatic fused heterocyclyl", "10-14-membered aromatic fused heterocyclyl" and the like, for example, a fused heterocyclyl formed by fusing benzene with 5-8-membered aromatic monocyclic heterocyclyl, a fused heterocyclyl formed by fusing 5-8-membered aromatic monocyclic heterocyclyl with 5-8-membered aromatic monocyclic heterocyclyl and the like; its specific example includes but is not limited to, for example, benzofuryl, benzoisofuryl, benzothienyl, indolyl, benzoxazolyl, benzimidazolyl, indazolyl, benzotriazolyl, quinolinyl, isoquinolinyl, pyridopyrazolyl, pyridopyrrolyl, pyrimidinopyrazolyl, pyrimidinopyrrolyl, pyridazinopyrazolyl, pyridazinopyrrolyl, acridinyl, phenanthridinyl, benzopyridazinyl, phthalazinyl, quinazolinyl, quinoxalyl, phenazinyl, pteridinyl, purinyl, naphthyridinyl and the like.

[0077] Said "6-14-membered partially saturated fused heterocyclyl" means a fused heterocyclyl containing at least one partially saturated ring or aromatic ring, for example, a group formed by fusing benzene with 3-8-membered partially saturated monocyclic heterocycle, a group formed by fusing 3-8-membered partially saturated monocyclic heterocyclyl with 3-8-membered saturated monocyclic heterocyclyl, a group formed by fusing 3-8-membered partially saturated monocyclic heterocyclyl with 3-8-membered partially saturated moncyclic heterocyclyl and the like, a group formed by fusing 3-8-membered partially saturated monocyclic heterocyclyl with 3-8-membered monocyclic cycloalkyl, a group formed by fusing 5-6-membered aromatic monocyclic heterocyclyl with 3-8-membered monocyclic cycloalkyl, a group formed by fusing 5-6-membered aromatic monocyclic heterocyclyl with 3-8-membered partially saturated monocyclic heterocyclyl and the like; its specific example includes but is not limited to, for example, 1,3-dihydrobenzofuryl, benzo[d][1,3]dioxolyl, isoindolinyl, chromanyl, 1,2,3,4-tetrahydropyrrolo[3,4-c]pyrrolyl, dihydropyrrolopyridinyl, dihydropyrrolopyrimidinyl, dihydropyrrolopyridazinyl, tetrahydropyrrolopyridinyl, tetrahydropyrrolopyrimidinyl, tetrahydropyrrolopyridazinyl and the like.

[0078] Said "6-14-membered saturated fused heterocyclyl" means a fused heterocyclyl, in which all of rings are saturated, for example, a group formed by fusing 3-8-membered saturated monocyclic heterocyclyl with 3-8-membered saturated monocyclic heterocyclyl, a group formed by fusing 3-8-membered saturated monocyclic cycloalkyl with 3-8-membered saturated monocyclic heterocyclyl and the like; its specific example includes but is not limited to, for example, cyclobutane-fused tetrahydropyrrolyl cyclopentane-fused tetrahydropyrrolyl azetidine-fused imidazolidinyl and the like.

[0079] In an embodiment of the preparation method of the present compound of general formula (I), the compound of general formula (I) wherein R6=H can be prepared, for example, according to the specific processes as illustrated in the following scheme:

wherein R1, R2, R3, R4, R5 and R7 are defined as hereinbefore, Hall and Hal2 represent halogen, which is each independently selected from the group consisting of F, Cl, Br and I, and Hall and Hal2 can be identical or different; Alk represents a lower alkyl, e.g. "C1-6alkyl", preferably "C1-4alkyl" and more preferably ethyl; "anhydride" is preferably an organic acid anhydride, for example, selected from but not limited to acetic anhydride, propionic anhydride, preferably acetic anhydride;


1. Preparation of Intermediate 1



[0080] Starting Material 1 and Starting Material 2 are dissolved in an alcohol organic solvent (which includes but is not limited to, for example, methanol, ethanol, isopropanol or t-butanol and the like, preferably ethanol and t-butanol). To the system is added a base (which includes an organic base and an inorganic base, preferably inorganic base, and which includes but is not limited to, for example, potassium hydroxide, sodium hydroxide, zinc hydroxide, calcium hydroxide, potassium carbonate, potassium bicarbonate, sodium carbonate or sodium bicarbonate and the like, preferably potassium carbonate and sodium carbonate). The reaction is conducted under heating to reflux with stirring until the starting material disapperance to produce Intermediate 1;

2. Preparation of Intermediate 2'



[0081] Intermediate 1 is added to an alcohol organic solvent (which includes but is not limited to, for example, methanol, ethanol, isopropanol or t-butanol and the like). To the mixture is added an reductant (preferably metal hydride, which includes but is not limited to, for example, sodium borohydride, lithium aluminum hydride or diborane and the like) to conduct a reduction reaction. The solvent is removed under a reduced pressure. To the resulting residue is added water. The resulting mixture is extracted with a halogenating hydrocarbon organic solvent (which includes but is not limited to, for example, chlorobenzene, dichlorobenzene, chloromethane or dichlormethane and the like). The organic phase is dried with a netrual drying agent (which is selected from, for example, but not limited to anhydrous calcium sulphate, anhydrous sodium sulfate or anhydrous magnesium sulfate and the like, preferably anhydrous sodium sulfate), and concentrated. To the resulting concentrated liquor is added an oxidant (which is selected from, for example, but not limited to potassium permanganate, potassium chlorate, manganese dioxide or ferric chloride and the like) in batch. The reaction is conducted with stirring to produce Intermediate 2';

3. Preparation of Intermediate 3'



[0082] Method 1: To a solution of Intermediate 2' and Starting Material 3 in an alcohol organic solvent (which is selected from, for example, but not limited to methanol, ethanol, isopropanol or t-butanol and the like) in a sealed vessel, is added an inorganic base (which is selected from, for example, but not limited to potassium hydroxide, sodium hydroxide, zinc hydroxide, calcium hydroxide, potassium carbonate, potassium bicarbonate, sodium carbonate or sodium bicarbonate and the like). The reaction was conducted at 130-160°C to produce Intermediate 3; or Method 2: Intermediate 2' is dissolved in a non protonic polar organic solvent (which is selected from, for example, but not limited to N,N-dimethylacetamide, N,N-dimethyl formamide, dimethyl sulfoxide or acetonitrile and the like) and Starting Material 3'. The reaction mixture is reacted in a microwave reactor until the starting material disappearance to produce Intermediate 3; and

4. Preparation of Formula (I')



[0083] Intermediate 3', and Starting Material 4 are dissolved in an organic solvent [selected from "an alcohol organic solvent (which includes but is not limited to, for example, methanol, ethanol, isopropanol or t-butanol and the like)", "an aromatic hydrocarbon organic solvent (which includes but is not limited to, for example, benzene, toluene or xylene and the like)" or a mixture thereof]. To the reaction system is added a solution of a catalyst (which is selected from, for example, but not limited to nickel catalyst, palladium catalyst, platinum catalyst or metal hydride catalyst and the like) and a base (e.g. potassium hydroxide, sodium hydroxide, zinc hydroxide, calcium hydroxide, potassium carbonate, potassium bicarbonate, sodium carbonate or sodium bicarbonate and the like). The resulting mixture is reacted under reflux in a nitrogen-protecting atmosphere to produce the compound of formula (I);

[0084] If necessary, a functional group that needs to be protected, such as hydroxy, amino and the like, can be protected; and afterwards can be deprotected according to the conventional method.

[0085] "A pharmaceutically acceptable salt" of the present compound of general formula (I) means a salt formed from an acidic functional group (e.g. -COOH, -OH, SO3H and the like) present in the compound of general formula (I) and a suitable inorganic or organic cationic ion (base), including, a salt formed with an alkali metal such as Na, K and the like, a salt formed with an alkaline-earth metal such as Ca, Mg and the like, an ammonium salt, and a salt formed with a nitrogen-containing organic base, wherein said organic base includes but is not limited to, for example, trimethylamine, triethylamine, tributylamine, pyridine, N,N-dimethylphenylamine, N-methylpiperidine, N-methylmorpholine, diethylamine, dicyclohexylamine, procaine, dibenzylamine, N-benzyl-β-benzeneethylamine, 1-diphenylhydroxymethylamine, N,N'-dibenzylethylenediamine and the like; and a salt formed from a basic functional group (e.g. -NH2 and the like) present in the compound of general formula (I) and a suitable inorganic or organic anionic ion (acid), including, a salt formed with an inorganic acid, such as hydrochloride, hydrobromide, sulfate and the like, a salt formed with an organic carboxylic acid, such as tartrate, formate, acetate, lactate, citrate, trichloroacetate, trifluoroacetate and the like, a salt formed with sulfonic acid, such as mesylate, benzenesulphonate, para-tosylate, napsylatenapsylate and the like.

[0086] The "stereoisomer" of the present compound of general formula (I) means all of possible stereoisomers caused in case that an asymmetric carbon atom or a carbon-carbon double bond is present in the compound of general formula (I), which includes enantiomer, diastereoisomer, racemate, cis-/trans-isomer, tauromer, geometric isomer, epimer and a mixture thereof, all of which fall into the scope of the present invention.

[0087] The present invention includes the "deuteride" of the compound of general formula (I), in case that the hydrogen atom in the compound is replaced in part or in a whole with its isotope deuterium (marked as D). The resulting deuteride compound also falls in the scope of the present invention.

[0088] The present compound of general forumla (I) and its pharmaceutically acceptable salt, stereoisomer and deuteride thereof can be administrated, for example, orally, parenterally (intravenously, intramuscularly, subcutaneously or rectally, and the like), pulmonarily, topically to a mammal e.g. human being. The daily dosage of the present compound can be in a range of about 20mg-500mg, preferably 50-300mg.

[0089] The present compound of general forumla (I), and its pharmaceutically acceptable salt, stereoisomer or deuteride thereof and one or more pharmaceutically acceptable carriers can be combined to form a pharmaceutically acceptable pharmaceutical formulation for oral, parenteral administration and the like to a patient in need thereof.

[0090] For the oral administration, the present compound of general formula (I), and its pharmaceutically acceptable salt, stereoisomer or deuteride thereof can be mixed with conventional filler, binder, disintegrant, lubricant and/or diluent and the like to formulate into a conventional solid preparation, such as tablet, capsule, pill, granule and the like; or an oral liquid preparation, such as an oral solution, an oral suspension, a syrup and the like. For the parenteral administration, the present compound of general formula (I), and its pharmaceutically acceptable salt, stereoisomer or deuteride thereof can be formulated into an injectable preparation, including an injection solution, a sterile injection powder and a concentrated injection solution. For preparing the injectable preparation, a conventional method in the pharmaceutical production can be used. For preparing the injectable preparation, an additive can be optionally added, depending on the nature of drug. The additive includes an osmotic regulator, a pH-value regulator, a solubilizer, a filler, an antioxidant, a bacteriostatic agent, an emulsifier, a suspending agent or the like.

[0091] The present compound of general formula (I), and its pharmaceutically acceptable salt, stereoisomer or deuteride thereof can be used to treat and/or prevent a proliferative disease, and can be administrated or used in combination with one or more other therapeutical agent(s), in particular, an antineoplastic agent and an immunosuppressive agent, said antineoplastic agent and said immunosuppressive agent are selected from antimetabolite, including but being not limited to capecitabine, gemcitabine, pemetrexed disodium; growth factor inhibitor, including but being not limited to pazopanib, imatinib, erlotinib, lapatinib, geftinat, vandetanib; antibody, including but being not limited to herceptin, bevacizumab; mitotic inhibitor, including but being not limited to paclitaxel, vinorelbine, docetaxel, doxorubicin; antineoplastic hormone, including but being not limited to letrozole, tamoxifen, fulvestrant, flutamide, triptorelin; alkylating agent, including but being not limited to cyclophosphamide, chlormethine, melphalan, chlorambucil, carmustine; metallic platinum, including but being not limited to carboplatin, cisplatin, oxaliplatin; topoismerase inhibitor, including but being not limited to topotecan, camptothecin, topotecan, irinotecanpto; immunosuppressive agents, including but being not limited to everolimus, sirolimus, torisel; purine analogues, including but being not limited to 6-mercaptopurine, 6-thioguanine, azathioprine; antibiotics, including but being not limited to actinomycin D, daunorubicin, adriamycin, mitoxantrone, bleomycin, plicamycin; platinum complex, including but being not limited to cisplatin, carboplatin; adrenal cortex inhibitors, including but being not limited to aminoglutethimide and the like. All of components to be administered or used in combination can be administered at the same time or successively and separately in a form of the single formulation or in a combination of the divided formulations.

[0092] The present invention also involves the use of a compound of general formula (I), and its pharmaceutically acceptable salt, stereoisomer or deuteride thereof in the manufacture of a medicament for treating and/or preventing a proliferative disease.

[0093] Said proliferative disease includes a cancer and a non-carcinomatous proliferative disease. Said cancer is selected from cerebroma, lung cancer, non-small cell lung cancer, squamous epithelial cell carcinoma, bladder carcinoma, gastric cancer, ovarian cancer, peritoneal cancer, pancreatic cancer, mammary cancer, head and neck cancer, cervical cancer, endometrial cancer, colorectal cancer, liver cancer, renal carcinoma, adenocarcinoma of esophagus, esophageal squamous cell cancer, solid tumor, non-Hodgkin lymphoma, glioma, glioblastoma multiforme, glioma sarcomatosum, prostate carcinoma, thyroid carcinoma, female genital tract cancer, carcinoma in situ, lymphoma, histiocytic lymphoma, neurofibromatosis, osteocarcinoma, cutaneous carcinoma, brain cancer, colon carcinoma, testis carcinoma, small cell lung cancer, gastrointestinal stromal tumor, prostate tumor, mast cell tumor, multiple myeloma, melanoma, neurogliocytoma, glioblastoma, astrocytoma, neuroblastoma, sarcoma and the like; said non-carcinomatous proliferative disease is selected from, for example, skin or prostate benign proliferations and the like.

[0094] It is demonstrated by experiment that the present compound is a dual PI3K and mTOR inhibitor, having an excellent antineoplastic effect; a good therapeutic effect on a proliferative disease; and a good pharmacokinetic characteristic.

Specific embodiments



[0095] Hereinafter, the present invention will be further illustrated in details by the following specific examples. It should be understood that the scope of the present invention is not limited by the following examples.

I. Preparation examples for the present compound


(I) Preparation of ethyl 4,6-dichloro-1,5-naphthyridine-3-carboxylate (starting material)



[0096] It was prepared according to the method disclosed in WO2010/038165 A1 as follows:

1. Preparation of 6-bromopyridine-3-amine



[0097] To a solution of 2-bromo-5-nitropyridine (64g, 0.317mol)/ethanol (1L) was successively added Fe powder (88g, 1.571mmol), concentrated hydrochloric acid (61mL) and water (287mL). The reaction mixture was reacted under reflux for 5h. The reaction mixture was cooled and filtered. The filtrate was concentrated and adjusted with a sodium bicarbonate solution to pH≈7-8, and re-filtered. The resulting filtrate was extracted with dichlormethane. The organic phase was dried with anhydrous sodium sulfate, and concentrated in a reduced pressure to produce 40.5g of the title compound as a pale-yellow solid in a yield of 74.4%.

2. Preparation of diethyl 2-((6-bromopyridine-3-ylamino)methylene)malonate



[0098] 6-bromopyridine-3-amine (74g, 0.43mol) and diethyl ethoxymethylenemalonate (100mL) were added ethanol (680mL). The mixture is reacted under heating to reflux for 5h. The reaction mixture was cooled. The solid was separated out and filtered by suction. The resulting solid was washed with petroleum ether to produce 125.4g of the title compound as a pale-yellow solid in a yield of 85.2%.

3. Preparation of ethyl 6-bromo-4-hydroxy-1,5-naphthyridine-3-carboxylate



[0099] To a boiling diphenylether (214mL) was added diethyl (2-((6-bromopyridine-3-ylamino)methylene)malonate (40g, 0.117mol) in 5 mins in batch. The mixture was heated to reflux for 45mins and TLC (ethyl acetate:petroleum ether=1:3) showed the starting material disappearance. The reaction mixture was cooled and poured into petroleum ether. The solid was separated out and filtered by suction to produce 24.6g of the title compound as an earthy yellow solid in a yield of 71.2%.

4. Preparation of ethyl 4,6-dichloro-1,5-naphthyridine-3-carboxylate



[0100] ethyl 6-bromo-4-hydroxy-1,5-naphthyridine-3-carboxylate (49.8g, 0.168mmol) and N,N-dimethylamine(8mL) were added tophosphorus oxychloride(400mL). The mixture was heated to reflux for 3h. The reaction mixture was cooled. Phosphorus oxychloride was removed by evaporation under a reduced pressure. The resulting residual was poured into an ice-water mixture. The resulting mixture was adjusted with a sodium bicarbonate solution to pH≈8, and then extracted with dichlormethane. The organic phase was dried with anhydrous sodium sulfate, concentrated, and purified with a silica-gel column chromatography (petroleum ether:ethyl acetate=3:1) to produce 20.2g of the title compound as a pale-yellow solid in a yield of 44.5%.

(II) Preparation of the present compound


Example 1: Preparation of 2-(6-aminopyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphth yridin-9(10H)-one (Compound 1)



[0101] 


1. Preparation of ethyl 6-chloro-4-(3-(trifluoromethyl)phenylamino)-1,5-naphthyridine-3-carboxylate



[0102] 



[0103] Ethyl 4,6-dichloro-1,5-naphthyridine-3-carboxylate (2.70g, 10.0mmol) and 3-trifluoromethylphenylamine (1.77g, 11.0mmol) were dissolved in t-butanol (50mL). To the resulting reaction system was added potassium carbonate (4.15g, 30.0mmol). The reaction was conducted with stirring under reflux for 3hrs. TLC (ethyl acetate:petroleum ether=1:3) showed the starting material disappearance. The reaction system was filtered by suction. The filter cake was washed with dichlormethane. The filter cake was discarded. The washing liquor and the filtrate were combined. The combined material was concentrated under a reduced pressure to obtain a solid, which was recrystallized with diether ether to produce 3.7g of the title compound as a pale-yellow solid in a yield of 93.5%.

2. Preparation of 6-chloro-4-(3-(trifluoromethyl)phenylamino)-1,5-naphthyridine-3-carbaldehyde



[0104] 



[0105] ethyl 6-chloro-4-(3-trifluoromethylphenylamino)-1,5-naphthyridin-3-carboxylate (2.5g, 6.3mmol) was added to ethanol (80mL). To this system was added sodium borohydride (0.95g, 25.1mmol) in batch. The mixture was stirred at room temperature for 24hrs. Ethanol was removed by evaporation under a reduced pressure. 20mL water was added to the resulting residue. The resulting mixture was extracted with dichlormethane. The organic phase was dried with anhydrous sodium sulfate and concentrated in a reduced pressure to produce 1.3g of the crude product. The crude product was dissolved in dichlormethane (20mL). To the resulting solution was added manganese dioxide (9.6g, 110.5mmol) in batch. The mixture was reacted under stirring at room temperature for 8h and filtered. The filter cake was washed with dichlormethane. The filter cake was discarded. The washing liquor and the filtrate were combined. The combined material was concentrated under a reduced pressure to obtain a solid, which was purified with a silica-gel column chromatography (ethyl acetate:petroleum ether=2:1) to produce 1.1 g of the title compound in a yield (two steps) of 49.7%.

3. Preparation of 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne



[0106] 



[0107] To a solution of 6-chloro-4-(3-(trifluoromethyl)phenylamino)-1,5-naphthyridine-3-carbaldehyde (1.0g, 2.84mmol) and ethyl 2-(diethoxyphosphoryl)acetate (1.78g, 7.98mmol) in t-butanol (70mL) in a 250mL sealed bottle was added potassium carbonate (2.36g, 17.1mmol). The mixture was reacted at 160°C for 36h. The reaction mixture was cooled. The reaction system was filtered by suction. The filter cake was washed with dichlormethane. The filter cake was discarded. The washing liquor and the filtrate were combined. The combined material was concentrated under a reduced pressure to obtain a solid, which was purified with a silica-gel column chromatography (ethyl acetate:petroleum ether=3:1) to produce 0.56g of the title compound in a yield of 52.5%.

4. Preparation of 2-(6-aminopyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphth yridin-9(10H)-one



[0108] 



[0109] 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne(418mg, 1.11mmol) and 5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine (368mg, 1.67mmol) were dissolved in toluene (40mL) and ethanol (10mL). To the resulting reaction system was added palladium tetrakis(triphenylphosphine) (12mg) and 2 N sodium carbonate solution (2mL). The resulting mixture was reacted under reflux in a nitrogen-protecting atmosphere for 12h. The reaction mixture was cooled to the room temperature and the filtered. The organic layer is concentrated in a reduced pressure and then dissolved in dichlormethane. The resulting solution was washed successively by water and saturated brine, dried over anhydrous sodium sulfate, concentrated, and then purified with a silica-gel column chromatography (ethyl acetate:petroleum ether=3:1) to produce 248 mg of the target compound in a yield of 51.5%.
Formula: C23H14F3N5O MW: 433.1 MS(M+1):434
1H NMR (d6-DMSO, 400 MHz): δ 9.14 (1H, s), 8.33 (2H, d), 8.22-8.10 (2H, m), 7.87-7.79 (2H, m), 7.72 (1H, t), 7.65-7.55 (1H, m), 6.95 (1H, d), 6.80 (1H, d), 6.40 (2H, s), 6.27 (1H, d).

[0110] According to the preparation method in Example 1 (for compound 1), Compound 3, i.e. (R)-2-(6-aminopyridin-3-yl)-10-(1-(2-hydroxypropanoyl)piperidin-4-yl)pyrido[3, 2-c][1,5]naphthyridin-9(10H)-one could be prepared.



[0111] Formula: C24H24N6O3 MW: 444.19 MS(M+H): 445.

[0112] According to the preparation method in Example 1 (for compound 1), Compound 4, i.e., (R)-10-(1-(2-hydroxypropanoyl)piperidin-4-yl)-2-(6-methoxypyridin-3-yl)pyrido [3,2-c][1,5]naphthyridin-9(10H)-one, could be prepared.



[0113] Formula: C25H25N5O4 MW: 459.19 MS(M+H): 460.

[0114] Example 2: Preparation of 2-(6-methoxypyridin-3-yl)-10-(4-(piperazin-1-yl)-3-(trifluoromethyl)phenyl)pyri do[3,2-c][1,5]naphthyridin-9(10H)-one (Compound 2)


1. Preparation of methyl 4-(4-(4-(tert-butoxycarbonyl)piperazin-1-yl)-3-(trifluoromethyl)phenylamino)-6-chloro-1,5-naphthyridine-3-carboxylate



[0115] 



[0116] Ethyl 4,6-dichloro-1,5-naphthyridine-3-carboxylate (0.5g, 1.84mmol) and tert-butyl 4-(4-amino-2-(trifluoromethyl)phenyl)piperazin-1-carboxylate (0.766g, 2.22mmol) were dissolved in a mixed solution of dichlormethane (5mL) and t-butanol (5mL). To the resulting reaction system was added potassium carbonate (0.612g, 4.43mmol). The mixture was reacted under stirring at room temperature for 24hrs. The reaction system was filtered by suction. The filter cake was washed with dichlormethane. The filter cake was discarded. The washing liquor and the filtrate were combined. The combined material was concentrated under a reduced pressure to obtain a solid, which was recrystallized with diether ether to produce 0.73g of the title compound as a pale-yellow solid in a yield of 70.1%.

2. Preparation of tert-butyl 4-(4-(6-chloro-3-(hydroxymethyl)-1,5-naphthyridin-4-ylamino)-2-(trifluorometh yl)phenyl)piperazin-1-carboxylate



[0117] 

methyl 4-(4-(4-(tert-butoxycarbonyl)piperazin-1-yl)-3-(trifluoromethyl)phenylamino)-6-chloro-1,5-naphthyridine-3-carboxylate (0.565g, 1.0mmol) was added to ethanol (10mL). To the system was added sodium borohydride(0.228g, 6mmol) in batch. The mixture was stirred at room temperature for 18hrs. Ethanol was removed under a reduced pressure. To the resulting residual was added to water (10mL). The resulting mixture was extracted with dichlormethane. The organic phase was dried with anhydrous sodium sulfate and concentrated in a reduced pressure to produce a title compound as solid crude, which was directly used in the next step.

3. Preparation of tert-butyl 4-(4-(6-chloro-3-formyl-1,5-naphthyridin-4-ylamino)-2-(trifluoromethyl)phenyl) piperazin-1-carboxylate



[0118] 



[0119] The crude tert-butyl 4-(4-(6-chloro-3-(hydroxymethyl)-1,5-naphthyridin-4-ylamino)-2-(trifluorometh yl)phenyl)piperazin-1-carboxylate (1mmol) directly obtained in the above step was dissolved in dichlormethane(10mL). To the resulting solution was added manganese dioxide (2.14g, 24.6mmol). The resulting mixture was reacted under stirring at room temperature 3h and filtered. The filter cake was washed with dichlormethane. The filter cake was discarded. The washing liquor and the filtrate were combined. The combined material was concentrated under a reduced pressure to obtain a solid, which was purified with a silica-gel column chromatography (ethyl acetate:petroleum ether=2:1) to produce 0.33g of the title compound in a yield (two steps) of 61.6%.

4. Preparation of tert-butyl 4-(4-(2-chloro-9-oxopyrido[3,2-c][1,5]naphthyridin-10(9H)-yl)-2-(trifluorometh yl)phenyl)piperazin-1-carboxylate



[0120] 



[0121] To a solution of tert-butyl 4-(4-(6-chloro-3-formyl-1,5-naphthyridin-4-ylamino)-2-(trifluoromethyl)phenyl) piperazin-1-carboxylate (2.5g, 4.66mmol) and ethyl 2-(diethoxyphosphoryl)acetate (2.93g, 13.1mmol) in t-butanol(125mL) in 250mL seal bottom was added potassium carbonate (3.87g, 28.0mmol). The mixture was reacted at 160°C for 36h. The reaction mixture was cooled. The reaction system was filtered by suction. The filter cake was washed with dichlormethane. The filter cake was discarded. The washing liquor and the filtrate were combined. The combined material was concentrated under a reduced pressure to obtain a solid, which was purified with a silica-gel column chromatography (ethyl acetate:petroleum ether=3:1) to produce 145 mg of the target compound in a yield of 5.6%.

5. Preparation of tert-butyl 4-(4-(2-(6-methoxypyridin-3-yl)-9-oxopyrido[3,2-c][1,5]naphthyridin-10(9H)-yl) -2-(trifluoromethyl)phenyl)piperazin-1-carboxylate



[0122] 

tert-butyl 4-(4-(2-(6-methoxypyridin-3-yl)-9-oxopyrido[3,2-c][1,5]naphthyridin-10(9H)-yl) -2-(trifluoromethyl)phenyl)piperazin-1-carboxylate (145mg, 0.26mmol) and 6-methoxy-3-pyridine boric acid (40mg, 0.26mmol) were dissolved in toluene(8mL) and ethanol (2mL). To the resulting reaction system was added palladium tetrakis(triphenylphosphine) (3mg) and 2N sodium carbonate solution (0.12mL). The resulting mixture was reacted under reflux in a nitrogen-protecting atmosphere for 4 h. The reaction mixture was cooled to room temperature, and filtered. The organic layer was separated out, concentrated in a reduced pressure, dissolved in dichlormethane, successively washed with water and saturated brine, dried over anhydrous sodium sulfate, concentrated, purified with a silica-gel column chromatography (ethyl acetate:petroleum ether=3:1) to produce 72 mg of the target compound in a yield of 43.8%.

6. Preparation of 2-(6-methoxypyridin-3-yl)-10-(4-(piperazin-1-yl)-3-(trifluoromethyl)phenyl)pyri do[3,2-c][1,5]naphthyridin-9(10H)-one



[0123] 

tert-butyl 4-(4-(2-(6-methoxypyridin-3-yl)-9-oxopyrido[3,2-c][1,5]naphthyridin-10(9H)-yl) -2-(trifluoromethyl)phenyl)piperazin-1-carboxylate (72mg, 0.114mmol) was dissolved in dichlormethane (8mL). To this system was introduced a gas of hydrogen chloride for 0.5h. The solid was separated out and filtered by suction. The resulting solid was successively washed with dichlormethane and diethylether. The resulting solid was dissolved in water. The resulting solution was adjusted with 1N sodium hydroxide solution to pH≈9, and then extracted with ethyl acetate. The organic phase was washed with saturated brine, dried over anhydrous sodium sulfate, concentrated in a reduced pressure to produce 54g of the title compound as a solid in a yield of 88.6%.
Formula: C28H23F3N6O2 MW: 532.18 MS(M+H): 532.9
1H NMR (CDCl3, 400 MHz): δ 9.04 (1H, s), 8.43 (1H, d), 8.14 (1H, d), 8.03 (1H, d), 7.95 (1H, d), 7.59 (1H, d), 7.47-7.38 (3H, m), 7.01 (1H, d), 6.72 (1H, d), 3.97 (3H, s), 3.05 (6H, br s), 2.98-2.89 (2H, m).

Example 3: Preparation of 2-methyl-2-(4-(9-oxo-2-(quinolin-3-yl)pyrido[3,2-c][1,5]naphthyridin-10(9H)-yl )phenyl)propanenitrile (Compound 5)



[0124] 


1. Preparation of methyl 6-chloro-4-(4-(2-(cyanopropan-2-yl)phenylamino) -1,5-naphthyridine-3-carboxylate



[0125] 



[0126] Ethyl 4,6-dichloro-1,5-naphthyridine-3-carboxylate (5.0g, 18.4mmol) and 2-(4-aminophenyl)-2-methylpropanenitrile (2.96g, 18.5mmol) were dissolved in 1,4-dioxane (80mL). The reaction was conducted with stirring under reflux for 4hrs. The reaction mixture was cooled, and concentrated in a reduced pressure. The resulting solid was dissolved with dichlormethane, successively washed with saturated aqueous sodium carbonate solution and saturated brine, dried over anhydrous sodium sulfate, and concentrated in a reduced pressure to produce 6.54g of the title compound as a pale-yellow solid in a yield of 90.2%.

2. Preparation of 2-(4-(6-chloro-3-(hydroxymethyl)-1,5-naphthyridin-4-ylamino)phenyl)-2-methyl propanenitrile



[0127] 



[0128] 6-chloro-4-(4-(2-(cyanopropan-2-yl)phenylamino)-1,5-naphthyridine-3-ethyl carboxylate (6.54g, 16.6mmol) was added to ethanol (200mL). To the system was added sodium borohydride(6.27g, 166mmol) in batch. The mixture was stirred at room temperature for 24hrs. Ethanol was removed under a reduced pressure. The resulting solution was adjusted with 1N diluted hydrochloric acid to a neutral pH, and extracted with ethyl acetate. The organic phase was dried with anhydrous sodium sulfate, concentrated in a reduced pressure to produce 5.8 g of the title compound as solid crude, which was directly used in the next step.

3. Preparation of 2-(4-(6-chloro-3-formyl-1,5-naphthyridin-4-ylamino)phenyl)-2-methylpropaneni trile



[0129] 



[0130] About 5.8 g of crude 2-(4-(6-chloro-3-(hydroxymethyl)-1,5-naphthyridin-4-ylamino)phenyl)-2-methyl propanenitrile obtained in the above step was dissolved in dichlormethane (80mL). To the resulting solution added manganese dioxide (46.2g, 0.53mol) in batch. The resulting mixture was reacted under stirring at room temperature for 30hrs and then filtered. The filter cake was washed with dichlormethane. The filter cake was discarded. The washing liquor and the filtrate were combined. The combined material was concentrated under a reduced pressure to obtain a solid. The resulting solid was purified with a silica-gel column chromatography (ethyl acetate:petroleum ether=2:1) to produce 2.3g of the title compound in a yield (two steps) of 39.5%.

4. Preparation of 2-(4-(2-chloro-9-oxopyrido[3,2-c][1,5]naphthyridin-10(9H)-yl)phenyl)-2-methyl propanenitrile



[0131] 



[0132] To a 250mL sealed bottle were successively added 2-(4-(6-chloro-3-formyl-1,5-naphthyridin-4-ylamino)phenyl)-2-methylpropaneni trile (1.0g, 2.85mmol), ethyl 2-(diethoxyphosphoryl)acetate (1.8g, 8.03mmol), t-butanol(70mL) and potassium carbonate(1.85g, 13.4mmol). The resulting mixture was reacted at 160°C for 48hrs. The reaction mixture was cooled. The reaction system was filtered by suction. The filter cake was washed with dichlormethane. The filter cake was discarded. The washing liquor and the filtrate were combined. The combined material was concentrated under a reduced pressure to obtain a solid. The resulting solid was purified with a silica-gel column chromatography (ethyl acetate:petroleum ether=1:1) to produce 0.31g of the title compound in a yield of 29.0%.

5. Preparation of 2-methyl-2-(4-(9-oxo-2-(quinolin-3-yl)pyrido[3,2-c][1,5]naphthyridin-10(9H)-yl )phenyl)propanenitrile



[0133] 

2-(4-(2-chloro-9-oxopyrido [3,2-c][1,5]naphthyridin-10(9H)-yl)phenyl)-2-methyl propanenitrile(310mg, 0.827mmol) and quinolin-3-yl boric acid (159mg, 0.919mmol) were dissolved in toluene (21mL) and ethanol (7mL). To the resulting reaction system was added palladium tetrakis(triphenylphosphine) (10mg) and 2N sodium carbonate solution (1.2mL). The resulting mixture was reacted under reflux in a nitrogen-protecting atmosphere for 8hrs. The reaction mixture was cooled to room temperature, and filtered. The organic layer was separated out, concentrated in a reduced pressure, dissolved in dichlormethane, successively washed with water and saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified with a silica-gel column chromatography (ethyl acetate:petroleum ether=1:1) to produce 108 mg of the target compound in a yield of 27.9%.
Formula: C30H21N5O MW: 467.17 MS(M+H): 467.9
1H-NMR(d6-DMSO, 400 MHz):δ 9.25 (1H, s), 8.76 (1H, d), 8.55 (1H, d), 8.46 (1H, d), 8.41-8.33 (2H, m), 8.04 (1H, d), 7.99 (1H, d), 7.85-7.77 (1H, m), 7.69-7.58 (3H, m), 7.43 (2H, d), 6.99 (1H, d), 1.60 (6H, s).

Example 4: Preparation of 2-(6-aminopyridin-3-yl)-10-(4-(piperazin-1-yl)-3-(trifluoromethyl)phenyl)pyrido [3,2-c][1,5]naphthyridin-9(10H)-one (Compound 6)hydrochloride



[0134] 


1. Preparation of tert-butyl 4-(4-(2-chloro-9-oxopyrido[3,2-c] [1,5]naphthyridin-10(9H)-yl)-2-(trifluorometh yl)phenyl)piperazin-1-carboxylate



[0135] 

tert-butyl 4-(4-(6-chloro-3-formyl-1,5-naphthyridin-4-ylamino)-2-(trifluoromethyl)phenyl) piperazin-1-carboxylate (524mg, 0.98mmol) was dissolved in 9mL N,N-dimethylacetamide and 6mL acetic anhydride. The resulting mixture was reacted in a microwave reactor at 160°C for 40min. The reaction mixture was cooled. A large portion of the solvent was removed under a reduced pressure. The resulting residue was purified with a silica-gel column chromatography (ethyl acetate) to produce 505 mg of the title compound as a white solid in a yield of 91.8%.

2. Preparation of tert-butyl 4-(4-(2-(6-aminopyridin-3-yl)-9-oxopyrido[3,2-c][1,5]naphthyridin-10(9H)-yl)-2 -(trifluoromethyl)phenyl)piperazin-1-carboxylate



[0136] 

tert-butyl 4-(4-(2-(6-methoxypyridin-3-yl)-9-oxopyrido[3,2-c][1,5]naphthyridin-10(9H)-yl) -2-(trifluoromethyl)phenyl)piperazin-1-carboxylate (302mg, 0.54mmol) and 5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine (240mg, 1.09mmol) were dissolved in toluene (6mL) and ethanol (2mL). To the resulting reaction system was added palladium tetrakis(triphenylphosphine) (13mg) and 2N sodium carbonate solution (1.6mL). The resulting mixture was reacted under reflux in a nitrogen-protecting atmosphere for 4 h. The reaction mixture was cooled to room temperature and filtered. The organic layer was separated out, concentrated in a reduced pressure, dissolved in dichlormethane, successively washed with water and saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified with a silica-gel column chromatography (ethyl acetate:petroleum ether=1:1) to produce 312 mg of the target compound in a yield of 93.5%.

3. Preparation of 2-(6-aminopyridin-3-yl)-10-(4-(piperazin-1-yl)-3-(trifluoromethyl)phenyl)pyrido [3,2-c] [1,5]naphthyridin-9(10H)-onehydrochloride



[0137] 

tert-butyl 4-(4-(2-(6-aminopyridin-3-yl)-9-oxopyrido[3,2-c][1,5]naphthyridin-10(9H)-yl)-2 -(trifluoromethyl)phenyl)piperazin-1-carboxylate (312mg, 0.505mmol) was dissolved in dichlormethane (8mL). To this system was introduced a gas of hydrogen chloride for 0.5h. The solid was separated out and filtered by suction. The resulting solid was successively washed with dichlormethane and diethylether to produce 271 mg of the title compound as a white solid in a yield of 96.8%.
Formula: C27H23ClF3N7O MW: 553.16 MS (M-HCl+H):518.0
1H-NMR(D2O, 400 MHz):δ 9.06 (1H, s), 8.28-8.19 (2H, m), 8.04 (1H, s), 7.88 (1H, d), 7.51 (1H, s), 7.38 (1H, d), 7.22 (1H, d), 7.05-6.93 (2H, m), 6.74 (1H, d), 3.32 (4H, br s), 3.22-3.10 (2H, m), 2.88-2.78 (2H, m).

Example 5: Preparation of 2-(4-(2-(6-aminopyridin-3-yl)-9-oxopyrido[3,2-c][1,5]naphthyridin-10(9H)-yl)ph enyl)-2-methylpropanenitrile (Compound 7)



[0138] 


1. Preparation of 2-(4-(2-chloro-9-oxopyrido [3,2-c][1,5]naphthyridin-10(9H)-yl)phenyl)-2-methyl propanenitrile



[0139] 



[0140] 2-(4-(6-chloro-3-formyl-1,5-naphthyridin-4-ylamino)phenyl)-2-methylpropaneni trile(1.3g, 3.71mmol) was dissolved in 12mL N,N-dimethylacetamide and 8mL acetic anhydride. The resulting mixture was reacted in a microwave reactor at 160°C for 60mins. The reaction mixture was cooled. A large portion of the solvent was removed under a reduced pressure. The resulting residue was purified with a silica-gel column chromatography (ethyl acetate) to produce 1.1 g of the title compound as a white solid in a yield of 79.0%.

2. 2-(4-(2-(6-aminopyridin-3-yl)-9-oxopyrido[3,2-c][1,5]naphthyridin-10(9H)-yl)ph enyl)-2-methylpropanenitrile



[0141] 



[0142] 2-(4-(2-chloro-9-oxopyrido [3,2-c][1,5]naphthyridin-10(9H)-yl)phenyl)-2-methyl propanenitrile (400mg, 1.07mmol) and 5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine (282mg, 1.28mmol) were dissolved in toluene (21mL) and ethanol (7mL). To the resulting reaction system was added palladium tetrakis(triphenylphosphine) (12mg) and 2N sodium carbonate solution (1.6mL). The resulting mixture was reacted under reflux in a nitrogen-protecting atmosphere for 8hrs. The reaction mixture was cooled to room temperature and filtered. The organic layer was separated out, concentrated in a reduced pressure, dissolved in dichlormethane, successively washed with water and saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified with a silica-gel column chromatography (ethyl acetate:petroleum ether=1:1) to produce 61 mg of the target compound in a yield of 13.2%.
Formula: C26H20N6O MW: 432.17 MS(M+H): 433.0
1H-NMR(d6-DMSO, 400 MHz):δ 9.11 (1H, s), 8.35-8.26 (2H, m), 8.10 (1H, d), 7.96 (1H, s), 7.60 (2H, d), 7.34 (2H, d), 7.24 (1H, d), 6.92 (1H, d), 6.39-6.27 (3H, m), 1.74 (6H, s).

Example 6: Preparation of 2-(4-(2-(6-methoxypyridin-3-yl)-9-oxopyrido[3,2-c][1,5]naphthyridin-10(9H)-yl) phenyl)-2-methylpropanenitrile (Compound 12)



[0143] 



[0144] The specific procedure was the same as those in Example 1, except for substituting
2-(4-(2-chloro-9-oxopyrido[3,2-c][1,5]naphthyridin-10(9H)-yl)phenyl)-2-methyl propanenitrile(200mg, 0.534mmol) and 6-methoxy-3-pyridine boric acid(100mg, 0.654mmol) respectively for 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne and 5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine to produce 182 mg of the target compound in a yield of 76.2%.
Formula: C27H21N5O2 MW: 447.17 MS(M+H): 448.2
1H-NMR (d6-DMSO, 400 MHz):δ 9.18 (1H, s), 8.42 (1H, d),, 8.33 (1H, d), 8.21 (1H, d) 7.85 (1H, d), 7.79 (1H, dd), 7.63 (2H, d), 7.37 (2H, d), 6.95 (1H, d), 6.78 (1H, d), 3.91 (3H, s), 1.75 (6H, s).

Example 7: Preparation of 2-(2-aminopyrimidin-5-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naph thyridin-9(10H)-one (Compound 13)



[0145] 


1. Preparation of 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridine-9(10H)-one



[0146] 



[0147] 6-chloro-4-(3-(trifluoromethyl)phenylamino)-1,5-naphthyridine-3-carbaldehyde (0.600g, 1.71mmol) was dissolved in 10mL N,N-dimethylacetamide and 6mL acetic anhydride. The resulting mixture was reacted in a microwave reactor at 160°C for 40min. The reaction mixture was cooled. A large portion of the solvent was removed under a reduced pressure. The resulting residue was purified with a silica-gel column chromatography (ethyl acetate) to produce 0.580 g of the title compound as a white solid in a yield of 90.1 %.

2. Preparation of 2-(2-aminopyrimidin-5-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naph thyridin-9(10H)-one



[0148] 



[0149] The specific procedure was the same as those in Example 1, Step 4, except for substituting 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne(300mg, 0.799mmol) and 5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyrimidin-2-amine (213mg, 0.963mmol) respectively for 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne and 5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine to produce 148 mg of the target compound in a yield of 42.7%.
Formula: C22H13F3N6O MW: 434.11 MS(M+H): 434.9
1H-NMR(d6-DMSO, 400 MHz):δ 9.14 (1H, s), 8.41-8.29 (2H, m), 8.15 (1H, d), 7.98 (2H, s), 7.82-7.70 (3H, m), 7.69-7.63 (1H, m), 7.03 (2H, s), 6.94 (1H, d).

Example 8: Preparation of 2-(2-methoxypyrimidin-5-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5] naphthyridin-9(10H)-one (Compound 14)



[0150] 



[0151] The specific processure was the same as those in Example 1, Step 4, except for substituting 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne(180mg, 0.479mmol) and 2-methoxypyrimidin-5-yl boric acid(89mg, 0.578mmol) respectively for 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne and 5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine to produce 165 mg of the target compound in a yield of 76.6%.
Formula: C23H14F3N5O2 MW: 449.11 MS(M+H): 450.2
1H-NMR(d6-DMSO, 400 MHz):δ 9.25 (1H, s), 8.50 (1H, d), 8.37 (1H, d), 8.33 (2H, s), 8.30 (1H, d), 7.85 (1H, s), 7.82 (1H, d), 7.74 (1H, t), 7.66 (1H, d), 7.00 (1H, d), 3.96 (3H, s).

Example 9: Preparation of N-(5-(9-oxo-10-(3-(trifluoromethyl)phenyl)-9,10-dihydropyrido[3,2-c][1,5]nap hthyridin-2-yl)pyridin-2-yl)acetamide (Compound 15)



[0152] 


1. Preparation of N-(5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-yl)acetamide



[0153] 



[0154] To a solution of 5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine (300mg, 1.36mmol) in dichlormethane(15mL) were successively added dimethylaminopyridine(17mg, 0.139mmol), triethylamine(0.21mL, 1.50mmol) and acetic anhydride(153mg, 1.50mmol). The resulting mixture was reacted under stirring at room temperature for several hours. Then the reaction mixture was diluted with dichlormethane, and washed with aqueous saturated ammonium chloride solution. The organic phase was dried with anhydrous magnesium sulfate, filtered, concentrated to produce 225 mg of the target compound in a yield of 63.1%.

2. Preparation of N-(5-(9-oxo-10-(3-(trifluoromethyl)phenyl)-9,10-dihydropyrido[3,2-c][1,5]napht hyridin-2-yl)pyridin-2-yl)acetamide



[0155] 



[0156] To N-(5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-yl)acetamide(225m g, 0.858mmol) were added 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne(300mg, 0.799mmol), palladium tetrakis(triphenylphosphine)(15mg) and 2N sodium carbonate solution(1.3mL). This system was reacted in a nitrogen-protecting atmosphere at 90 °C for 16hrs. The reaction mixture was cooled to room temperature and filtered. The organic layer was separated out, concentrated in a reduced pressure, dissolved in dichlormethane, successively washed with water and saturated brine, dried over anhydrous sodium sulfate, concentrated and purified with a silica-gel column chromatography (ethyl acetate) to produce 160 mg of the target compound in a yield of 42.2%.
Formula: C25H16F3N5O2 MW: 475.13 MS(M+H): 476.2
1H-NMR(d6-DMSO, 400 MHz):δ 10.65 (1H, s), 9.23 (1H, s), 8.46 (1H, d), 8.37 (1H, d), 8.34 (1H, d), 8.32 (1H, d), 7.98 (1H, d), 7.86 (1H, s), 7.80 (1H, d), 7.74 (1H, t), 7.66 (1H, d), 7.31 (1H, dd), 6.98 (1H, d), 2.12 (3H, s).

Example 10: Preparation of 5-(9-oxo-10-(3-(trifluoromethyl)phenyl)-9,10-dihydropyrido[3,2-c][1,5]naphth yridin-2-yl)-2-cyanopyridine (Compound 16)



[0157] 



[0158] The specific processure was the same as those in Example 9 (for the preparation of compound 15), Step 2, except for substituting 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne(188mg, 0.50mmol) and 5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-2-cyanopyridine(138mg, 0.60mmol) respectively for 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne and N-(5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-yl)acetamide to produce 128 mg of the target compound in a yield of 57.8%.
Formula: C24H12F3N5O MW: 443.1 MS(M+H): 444.1
1H-NMR(d6-DMSO, 400 MHz):δ 9.27 (1H, s), 8.55 (1H, d), 8.49 (1H, d), 8.40 (1H, d), 8.37 (1H, d), 7.97 (1H, d), 7.89 (1H, d), 7.81 (1H, s), 7.77 (1H, t), 7.71-7.62 (2H, m), 7.00 (1H, d).

Example 11: Preparation of 2-(1H-pyrrolo[2,3-b]pyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c] [1,5]naphthyridin-9(10H)-one (Compound 17)



[0159] 


1. Preparation of tert-butyl 3-(9-oxo-10-(3-(trifluoromethyl)phenyl)-9,10-dihydropyrido[3,2-c][1,5]naphthyr idin-2-yl)-1H-pyrrolo[2,3-b]pyridin-1-carboxylate



[0160] 



[0161] 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne(137mg, 0.365mmol) and tert-butyl 3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-pyrrolo[2,3-b]pyridin-1-carb oxylate (151mg, 0.439mmol) were dissolved in toluene(6mL) and ethanol (2mL). To the resulting reaction system was added palladium tetrakis(triphenylphosphine)(10mg) and 2N sodium carbonate solution(0.5mL). The resulting mixture was reacted under reflux in a nitrogen-protecting atmosphere for 6 h. The reaction mixture was cooled to room temperature and filtered. The organic layer was separated out, concentrated in a reduced pressure, dissolved in dichlormethane, successively washed with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated to dryness to produce 193 mg of the target compound in a yield of 95.2%.

2. Preparation of 2-(1H-pyrrolo[2,3-b]pyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1 ,5]naphthyridin-9(10H)-one



[0162] 



[0163] The crude tert-butyl 3-(9-oxo-10-(3-(trifluoromethyl)phenyl)-9,10-dihydropyrido[3,2-c][1,5]naphthyr idin-2-yl)-1H-pyrrolo[2,3-b]pyridin-1-carboxylate (193mg, 0.347mmol) directly obtained in the above step was dissolved in dichlormethane(10mL). To this system was introduced a gas of hydrogen chloride for 0.5h. The solid was separated out and filtered by suction. The resulting solid was successively washed with dichlormethane and diethylether. The resulting material was dissolving in water. The resulting solution was adjusted with 1N sodium hydroxide solution to pH=9, and then extracted with ethyl acetate. The organic phase was washed with saturated brine, dried over anhydrous sodium sulfate, filtered by suction, concentrated, and purified with a silica-gel column chromatography (ethyl acetate) to produce 44 mg of the target compound in a yield of 27.7%.
Formula: C25H14F3N5O MW: 457.12 MS(M+H): 458.2
1H-NMR(d6-DMSO, 400 MHz):δ 12.16 (1H, s), 9.15 (1H, s), 8.39 (1H, d), 8.35 (1H, d), 8.31-8.24 (2H, m), 8.18 (1H, d), 7.83 (1H, s), 7.77-7.65 (3H, m), 7.17 (1H, dd), 6.96 (1H, d), 6.73 (1H, s).

Example 12: Preparation of 2-(6-(hydroxymethyl)pyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][ 1,5]naphthyridin-9(10H)-one (Compound 18)



[0164] 


1. Preparation of (5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-yl)methanol



[0165] 



[0166] To 1,4-dioxane(20mL) was successively added(5-bromopyridin-2-yl)methanol (376mg, 2.0mmol), bis(pinacolato)diboron (762mg, 3.0mmol), potassium acetate (504mg, 5.14mmol) and [1,1'-bis(diphenylphosphino)ferrocene] palladium(II) chloride dichlormethane complex(20mg). The resulting mixture was reacted under stirring in a nitrogen-protecting atmosphere at 90°C for 12hrs. The reaction mixture was cooled and directly used in the next step without a further treatment.

2. Preparation of 2-(6-(hydroxymethyl)pyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][ 1,5]naphthyridin-9(10H)-one



[0167] 



[0168] To the cooled reaction liquor of ((5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-yl)methanol) obtained in the above step, were added 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne(315mg, 0.84mmol), palladium tetrakis(triphenylphosphine)(15mg) and 2N sodium carbonate solution(3.0mL). This system was reacted in a nitrogen-protecting atmosphere at 90 °C for 16hrs. The reaction mixture was cooled to room temperature and filtered. The organic layer was separated out, concentrated in a reduced pressure, dissolved in dichlormethane, successively washed with water and saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified with a silica-gel column chromatography (ethyl acetate) to produce 189 mg of the target compound in a yield of 50.0%.
Formula: C24H15F3N4O2 MW: 448.11 MS(M+H): 449.2
1H-NMR(d6-DMSO, 400 MHz):δ 9.24 (1H, s), 8.58 (1H, d), 8.49 (1H, d), 8.38 (1H, d), 8.35 (1H, d), 7.89-7.83 (2H, m), 7.73 (1H, t), 7.65 (1H, d), 7.35 (1H, d), 7.25 (1H, dd), 6.99 (1H, d), 5.51 (1H, t), 4.58 (2H, d).

Example 13: Preparation of 2-(6-(4-methylpiperazin-1-yl)pyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyri do[3,2-c][1,5]naphthyridin-9(10H)-one (Compound 19)



[0169] 




1. Preparation of 1-(5-bromopyridin-2-yl)-4-methylpiperazine



[0170] 



[0171] 2,5-dibromopyridine(2.00g, 8.44mmol) and 1-methylpiperazine(3.00mL) were stirred at 110°C for 2hrs. An excess of 1-methylpiperazine was removed by evaporation in vacuum under a reduced pressure. To the resulting residue was added a saturated sodium bicarbonate solution. The resulting solution was extracted with ethyl acetate. The organic phase was dried with anhydrous sodium sulfate, and filtered. The organic solvent was removed under a reduced pressure to produce 1.45g of the title compound as a brown solid in a yield of 67.1%.

2. Preparation of 1-methyl-4-(5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-yl)piperazi ne



[0172] 



[0173] To 1,4-dioxane(20mL) was successively added 1-(5-bromopyridin-2-yl)-4-methylpiperazine (0.67g, 2.62mmol), bis(pinacolato)diboron (1.00g, 3.94mmol), potassium acetate (0.65g, 6.63mmol) and [1,1'-bis(diphenylphosphino)ferrocene] palladium(II) chloride dichlormethane complex(15mg). The resulting mixture was reacted under stirring in a nitrogen-protecting atmosphere at 90°C for 12hrs. The reaction mixture was cooled and directly used in the next step without a further treatment.

3. Preparation of 2-(6-(4-methylpiperazin-1-yl)pyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido [3,2-c][1,5]naphthyridin-9(10H)-one



[0174] 



[0175] To the cooled reaction liquor of (1-methyl-4-(5-(4,4,5,5-tetramethyl-1,3 ,2-dioxaborolan-2-yl)pyridin-2-yl)piperaz ine) obtained in the above step, were added 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne (0.68g, 1.81mmol), palladium tetrakis(triphenylphosphine) (15mg) and 2N sodium carbonate solution (3.9mL). This system was reacted in a nitrogen-protecting atmosphere at 90 °C for 16 h, cooled to the room temperature and filtered. The organic layer was separated out, concentrated in a reduced pressure, dissolved in dichlormethane, successively washed with water and saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified with a silica-gel column chromatography (ethyl acetate) to produce 518 mg of the target compound in a yield of 55.6%.
Formula: C28H23F3N6O MW: 516.19 MS(M+H): 517.3
1H-NMR(CDCl3, 400 MHz): δ 8.99 (1H, s), 8.35 (1H, d), 8.33 (1H, d), 8.02 (1H, d), 7.94 (1H, d), 7.76 (1H, d), 7.69 (1H, s), 7.63 (1H, t), 7.42 (1H, d), 6.99 (1H, d), 6.94 (1H, dd), 6.46 (1H, d), 3.75-3.67 (4H, m), 2.64-2.56 (4H, m), 2.40 (3H, s).

Example 14: Preparation of 2-(6-(1H-pyrazol-1-yl)pyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one (Compound 20)



[0176] 


1. Preparation of 5-bromo-2-(1H-pyrazol-1-yl)pyridine



[0177] 



[0178] To a solution of 60% sodium hydride (1.20g, 30.0mmol) in N,N-dimethyl formamide (40mL) was added pyrazole (2.05g, 30.1mmol) in batch. The resulting mixture was reacted under stirring at room temperature for an hour. Then to the reaction system was added 2,5-dibromopyridine(4.75g, 20.1mmol). The resulting mixture was reacted under stirring at 100°C for 2hrs. The reaction mixture was cooled and poured into an ice water, The solid was separated out and filtered by suction. The resulting solid was dired in vacuum and recrystallized with n-hexane to produce 3.31 g of the title compound as brown solid in a yield of 73.6%.

2. Preparation of 2-(1H-pyrazol-1-yl)-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridine



[0179] 



[0180] To 1,4-dioxane(20mL) was successively added 5-bromo-2-(1H-pyrazol-1-yl)pyridine (0.45g, 2.01mmol), bis(pinacolato)diboron (0.76g, 2.99mmol), potassium acetate (0.51g, 5.20mmol) and [1,1'-bis(diphenylphosphino)ferrocene] palladium(II) chloride dichlormethane complex(15mg). The resulting mixture was reacted under stirring in a nitrogen-protecting atmosphere at 90°C for 5hrs. The reaction mixture was cooled and directly used in the next step without a further treatment.

3. Preparation of 2-(6-(1H-pyrazol-1-yl)pyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c] [1,5]naphthyridin-9(10H)-one



[0181] 



[0182] To the cooled reaction liquor of (2-(1H-pyrazol-1-yl)-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridine) obtained in the above step, were added 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne (300mg, 0.799mmol), palladium tetrakis(triphenylphosphine) (15mg) and 2N sodium carbonate solution (3mL). This system was reacted in a nitrogen-protecting atmosphere at 90 °C for 16hrs. The reaction mixture was cooled to room temperature and filtered. The organic layer was separated out, concentrated in a reduced pressure, dissolved in dichlormethane, successively washed with water and saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified with a silica-gel column chromatography (ethyl acetate) to produce 291 mg of the target compound in a yield of 75.2%.
Formula: C26H15F3N6O MW: 484.13 MS(M+H): 485.2
1H-NMR(d6-DMSO, 400 MHz):δ 9.24 (1H, s), 8.62 (1H, d), 8.54 (1H, d), 8.50 (1H, d), 8.38 (1H, d), 8.36 (1H, d), 7.93 (1H, d), 7.91-7.87 (2H, m), 7.79-7.72 (2H, m), 7.65 (1H, d), 7.38 (1H, dd), 6.99 (1H, d), 6.62 (1H, t).

Example 15: Preparation of 2-(4-(2-(2-methoxypyrimidin-5-yl)-9-oxopyrido[3,2-c][1,5]naphthyridin-10(9 H)-yl)phenyl)-2-methylpropanenitrile (Compound 21)



[0183] 



[0184] The specific procedure was the same as those in Example 1, Step 4, except for substituting 2-(4-(2-chloro-9-oxopyrido[3,2-c][1,5]naphthyridin-10(9H)-yl)phenyl)-2-methyl propanenitrile(166mg, 0.443mmol) and 2-methoxypyrimidin-5-yl boric acid respectively for 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne and 5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine to produce 157 mg of the target compound in a yield of 79.0%.
Formula: C26H20N6O2 MW: 448.16 MS(M+H): 449.2
1H-NMR(d6-DMSO, 400 MHz):δ 9.19 (1H, s), 8.45 (1H, d),, 8.37 (2H, s), 8.32 (1H, d), 8.27 (1H, d), 7.63 (2H, d), 7.37 (2H, d), 6.96 (1H, d), 3.96 (3H, s), 1.78 (6H, s).

Example 16: Preparation of 2-(6-(methylsulfonyl)pyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c ][1,5]naphthyridin-9(10H)-one (Compound 22)



[0185] 




1. Preparation of 5-bromo-2-(methylthio)pyridine



[0186] 



[0187] 2,5-dibromopyridine (3.0g, 12.66mmol) was dissolved in N,N-dimethyl formamide (20mL). To the resulting solution was added sodium methyl mercaptide (1.1g, 15.69mmol). The resulting mixture was placed in an ice-bath in the nitrogen protection to react with stirring for 16hrs. The reaction mixture was poured into water (150mL). The solid was separated out and filtered by suction. The resulting solid was dired to produce 2.5g of the title compound as whilte solid crude, which was directly used in the next step.

2. Preparation of 5-bromo-2-(methylsulfonyl)pyridine



[0188] 



[0189] 2.5g of the crude 5-bromo-2-(methylthio)pyridine directly obtained in the above step was dissolved in a mixed solution of isopropanol (10mL) and water (5mL). To the resulting mixture was added a compound salt of potassium monopersulfate (15.7g, 25.5mmol). The resulting material was stirred overnight at room temperature and filtered. The filtrate was concentrated and then dissolved in ethyl acetate. The resulting material was successively washed with water and saturated brine, dried with anhydrous magnesium sulfate, and filtered. The filtrate was concentrated in a reduced pressure, and then purified with a silica-gel column chromatography (petroleum ether:ethyl acetate=5:1) to produce 0.43 g of the title compound as a white solid in a yield (two steps) of 14.4%.

3. Preparation of 2-(methylsulfonyl)-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridine



[0190] 



[0191] To 1,4-dioxane (20mL) was successively added 5-bromo-2-(methylsulfonyl)pyridine (0.200g, 0.847mmol), bis(pinacolato)diboron (433mg, 1.71mmol), potassium acetate (167mg, 1.70mmol) and [1,1'-bis(diphenylphosphino)ferrocene] palladium(II) chloride dichlormethane complex(50mg). The resulting mixture was reacted under stirring in a nitrogen-protecting atmosphere at 90°C for 5hrs. The reaction mixture was cooled and directly used in the next step without a further treatment.

4. Preparation of 2-(6-(methylsulfonyl)pyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][ 1,5]naphthyridin-9(10H)-one



[0192] 



[0193] To the cooled reaction liquor of 2-(methylsulfonyl)-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridine obtained in the above step, were added 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne (319mg, 0.85mmol), palladium tetrakis(triphenylphosphine) (15mg) and 2N sodium carbonate solution(1.3mL). This system was reacted in a nitrogen-protecting atmosphere at 90 °C for 16hrs. The reaction mixture was cooled to room temperature and filtered. The organic layer was separated out, concentrated in a reduced pressure, dissolved in dichlormethane, successively washed with water and saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified with a silica-gel column chromatography (ethyl acetate) to produce 58 mg of the target compound in a yield of 13.7%.
Formula: C24H15F3N4O3S MW: 496.08 MS(M+H): 497.1
1H-NMR(d6-DMSO, 400 MHz):δ 9.35 (1H, s), 8.74 (1H, d), 8.63 (1H, d), 8.50 (1H, d), 8.44 (1H, d), 7.99-7.89 (3H, m), 7.82 (1H, t), 7.73 (1H, d), 7.68 (1H, dd), 7.07 (1H, d), 3.36 (3H, s).

Example 17: Preparation of 2-(6-methoxypyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]na phthyridin-9(10H)-one (Compound 23)



[0194] 



[0195] The specific processure was the same as those in Example 1, Step 4, except for substituting 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne(100mg, 0.266mmol) and 6-methoxy-3-pyridine boric acid respectively for 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne and 5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine to produce 82 mg of the target compound in a yield of 68.8%.
Formula: C24H15F3N4O2 MW: 448.11 MS(M+H): 449.1
1H-NMR(d6-DMSO, 400 MHz):δ 9.22 (1H, s), 8.45 (1H, d), 8.39-8.34 (2H, m), 8.29 (1H, d), 7.92-7.84 (2H, m), 7.74 (1H, t), 7.63 (1H, d), 7.10 (1H, dd), 6.99 (1H, d), 6.66 (1H, d), 3.90 (3H, s).

Example 18: Preparation of 2-(6-morpholinopyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]n aphthyridin-9(10H)-one (Compound 24)



[0196] 



[0197] The specific processure was the same as those in Example 1, Step 4, except for substituting 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne (150mg, 0.399mmol) and 6-morpholinopyridin-3-yl boric acid (100mg, 0.481mmol) respectively for 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne and 5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine to produce 78 mg of the target compound in a yield of 38.8%.
Formula: C27H20F3N5O2 MW: 503.16 MS(M+H): 504.2
1H-NMR(d6-DMSO, 400 MHz):δ 9.16 (1H, s), 8.37 (1H, d), 8.34 (1H, d), 8.28 (1H, d), 8.22 (1H, d), 7.90 (1H, d), 7.84 (1H, s), 7.73 (1H, t), 7.62 (1H, d), 7.00 (1H, dd), 6.97 (1H, s), 6.62 (1H, d), 3.71 (4H, t), 3.53 (4H, t).

Example 19: Preparation of 2-(5-methoxypyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naph thyridin-9(10H)-one (Compound 25)



[0198] 



[0199] The specific processure was the same as those in Example 1, Step 4, except for substituting 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne (150mg, 0.399mmol) and 3-methoxy-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-pyridine (113mg, 0.48mmol) respectively for 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne and 5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine to produce 87 mg of the target compound in a yield of 48.6%.
Formula: C24H15F3N4O2 MW: 448.11 MS(M+H): 449.1
1H-NMR (d6-DMSO, 400 MHz):δ 9.26 (1H, s), 8.51 (1H, d), 8.37 (1H, d), 8.35 (1H, d), 8.31 (1H, d), 7.85-7.75 (3H, m), 7.74-7.63 (2H, m), 7.24 (1H, s), 6.99 (1H, d), 3.89 (3H, s).

Example 20: Preparation of 2-(2-methoxypyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naph thyridin-9(10H)-one (Compound 26)



[0200] 



[0201] The specific processure was the same as those in Example 1, Step 4, except for substituting 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne(150mg, 0.399mmol) and 2-methoxypyridin-3-yl boric acid(74mg, 0.484mmol) respectively for 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne and 5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine to produce 107 mg of the target compound in a yield of 59.9%.
Formula: C24H15F3N4O2 MW: 448.11 MS(M+H): 449.1
1H-NMR(d6-DMSO, 400 MHz):δ 9.25 (1H, s), 8.43 (1H, d), 8.37 (1H, d), 8.24 (1H, d), 8.20 (1H, dd), 7.82 (1H, s), 7.74 (1H, d), 7.65 (1H, t), 7.60 (1H, d), 6.98 (1H, d), 6.84 (1H, dd), 6.72 (1H, dd), 3.90 (3H, s).

Example 21: Preparation of 10-(3-(trifluoromethyl)phenyl)-2-(6-(trifluoromethyl)pyridin-3-yl)pyrido[3,2-c][ 1,5]naphthyridin-9(10H)-one (Compound 27)



[0202] 



[0203] The specific processure was the same as those in Example 1, Step 4, except for substituting 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne(150mg, 0.399mmol) and 6-(trifluoromethyl)pyridin-3-yl boric acid(92mg, 0.482mmol) respectively for 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne and 5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine to produce 102 mg of the target compound in a yield of 52.6%.
Formula: C24H12F6N4O MW: 486.09 MS(M+H): 487.1
1H-NMR(d6-DMSO, 400 MHz):δ 9.29 (1H, s), 8.66 (1H, s), 8.57 (1H, d), 8.42 (1H, d), 8.38 (1H, d), 7.88 (1H, d), 7.85-7.75 (3H, m), 7.69 (1H, d), 7.59 (1H, d), 7.01(1H, d).

Example 22: Preparation of 2-(6-methylpyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphth yridin-9(10H)-one (Compound 28)



[0204] 



[0205] The specific processure was the same as those in Example 1, Step 4, except for substituting 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne(225mg, 0.599mmol) and 6-methylpyridin-3-yl boric acid(99mg, 0.723mmol) respectively for 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne and 5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine to produce 160 mg of the target compound in a yield of 61.8%.
Formula: C24H15F3N4O MW: 432.12 MS(M+H): 433.2
1H-NMR(CDCl3, 400 MHz):δ 9.06 (1H, s), 8.61 (1H, s), 8.46 (1H, d), 8.05 (1H, d), 8.03 (1H, d), 7.76 (1H, d), 7.66 (1H, d), 7.63 (1H, s), 7.47(1H, d), 7.07-7.00 (3H, m), 2.60 (3H, s).

Example 23: Preparation of 2-(3,5-dimethylisoxazol-4-yl)-10-(3-(trifluoromethyl)phenyl)pyrido [3,2-c][1,5]n aphthyridin-9(10H)-one (Compound 29)



[0206] 



[0207] The specific processure was the same as those in Example 1, Step 4, except for substituting 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne(188mg, 0.50mmol) and 3,5-dimethyl-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-isoxazole (134mg, 0.575mmol) respectively for 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne and 5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine to produce 112 mg of the target compound in a yield of 51.4%.
Formula: C23H15F3N4O2 MW: 436.11 MS(M+H): 437.1
1H-NMR(d6-DMSO, 400 MHz):δ 9.26 (1H, s), 8.45 (1H, d), 8.37 (1H, d), 7.76 (1H, d), 7.65-7.55 (3H, m), 7.50 (1H, d), 7.00 (1H, d), 2.11 (3H, s), 1.89 (3H, s).

Example 24: Preparation of 2-(5-fluoropyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphth yridin-9(10H)-one (Compound 30)



[0208] 



[0209] The specific processure was the same as those in Example 1, Step 4, except for substituting 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne(200mg, 0.53mmol) and 5-fluoropyridin-3-yl boric acid(90mg, 0.64mmol) respectively for 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne and 5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine to produce 162 mg of the target compound in a yield of 69.8%.
Formula: C23H12F4N4O MW: 436.09 MS(M+H): 437.1
1H-NMR(d6-DMSO, 400 MHz):δ 9.29 (1H, s), 8.61 (1H, d), 8.55 (1H, d), 8.50 (1H, t), 8.42 (1H, d), 8.39 (1H, d), 7.87 (1H, s), 7.81 (1H, d), 7.75 (1H, t), 7.69 (1H, d), 7.14 (1H, dt), 7.01 (1H, d).

Example 25: Preparation of 10-(3-(trifluoromethyl)phenyl-2-(5-(trifluoromethyl)pyridin-3-yl)pyrido[3,2-c][1 ,5]naphthyridin-9(10H)-one (Compound 31)



[0210] 


1. Preparation of 3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-5-(trifluoromethyl)pyridine



[0211] 



[0212] To 1,4-dioxane (20mL) was successively added 3-bromo-5-trifluoromethylpyridine (273mg, 1.21mmol), bis(pinacolato)diboron (460mg, 1.81mmol), potassium acetate (300mg, 3.06mmol) and [1,1'-bis(diphenylphosphino)ferrocene] palladium(II) chloride dichlormethane complex (15mg). The resulting mixture was reacted under stirring in a nitrogen-protecting atmosphere at 90°C for 12hrs. The reaction mixture was cooled and directly used in the next step without a further treatment.

2. Preparation of 10-(3-(trifluoromethyl)phenyl-2-(5-(trifluoromethyl)pyridin-3-yl)pyrido[3,2-c][1 ,5]naphthyridin-9(10H)-one



[0213] 



[0214] To the cooled reaction liquor of 3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-5-(trifluoromethyl)pyridine obtained in the above step, were added 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne (300mg, 0.799mmol), palladium tetrakis(triphenylphosphine) (15mg) and 2N sodium carbonate solution (1.8mL). This system was reacted in a nitrogen-protecting atmosphere at 90 °C for 16hrs. The reaction mixture was cooled to room temperature and filtered. The organic layer was separated out, concentrated in a reduced pressure, dissolved in dichlormethane, successively washed with water and saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified with a silica-gel column chromatography (ethyl acetate) to produce 72 mg of the target compound in a yield of 18.5%.
Formula: C24H12F6N4O MW: 486.09 MS(M+H): 487.1
1H-NMR(d6-DMSO, 400 MHz):δ 9.29 (1H, s), 9.00 (1H, s), 8.55 (1H, d), 8.44 (1H, d), 8.41-8.36 (2H, m), 8.12 (1H, s), 7.78 (1H, s), 7.76-7.71 (3H, m), 7.01 (1H, d).

Example 26: Preparation of 2-(1-methyl-1H-pyrazol-5-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]n aphthyridin-9(10H)-one (Compound 32)



[0215] 



[0216] The specific processure was the same as those in Example 1, Step 4, except for substituting 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c] [1,5]naphthyridin-9(10H)-o ne(188mg, 0.50mmol) and 1-methyl-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H- pyrazole (127mg, 0.61mmol) respectively for 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne and 5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine to produce 105 mg of the target compound in a yield of 49.8%.
Formula: C22H14F3N5O MW: 421.12 MS(M+H): 422.1
1H-NMR(d6-DMSO, 400 MHz): δ 9.27 (1H, s), 8.48 (1H, d), 8.38 (1H, d), 7.95 (1H, d), 7.74 (1H, s), 7.64-7.55 (3H, m), 7.35 (1H, d), 7.00 (1H, d), 6.05 (1H, d), 3.58 (3H, s).

Example 27: Preparation of 2-(thiazol-5-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9( 10H)-one (Compound 33)



[0217] 



[0218] The specific processure was the same as those in Example 1, Step 4, except for substituting 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(110H)-o ne (188mg, 0.50mmol) and 5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-thiazole (125mg, 0.59mmol) respectively for 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne and 5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine to produce 71 mg of the target compound in a yield of 33.6%.
Formula: C21H11F3N4OS MW: 424.06 MS(M+H): 425.1
1H-NMR(d6-DMSO, 400 MHz): δ 9.22 (1H, s), 9.10 (1H, s), 8.46 (1H, d), 8.37 (1H, s), 8.35 (1H, d), 8.28 (1H, d), 7.85-7.80 (2H, m), 7.75 (1H, t), 7.59 (1H, d),

Example 28: Preparation of 3-methyl-5-(9-oxo-10-(3 -(trifluoromethyl)phenyl)-9,10-dihydropyrido[3,2-c][1,5 ]naphthyridin-2-yl)-2-cyanopyridine (Compound 34)



[0219] 


1. Preparation of 5-bromo-3-methyl-2-cyanopyridine



[0220] 



[0221] To a solution of 2,5-dibromo-3-methylpyridine (5.0g, 19.9mmol) in N,N-dimethyl formamide (20mL) was added cuprous cyanide (1.8g, 20.0mmol). The resulting mixture was reacted at 120°C under stirring for 12hrs. The reaction mixture was cooled, to which water was added. The resulting mixture was extracted with ethyl acetate. The organic phase was washed with saturated brine, dried with anhydrous sodium sulfate, and filtered. The filtrate was concentrated and purified with a silica-gel column chromatography (petroleum ether:ethyl acetate=5:1) to produce 2.4 g of the title compound as a white solid in a yield of 61.3%.

2. Preparation of 3-methyl-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-2-cyanopyridine



[0222] 



[0223] To 1,4-dioxane (20mL) was successively added 5-bromo-3-methyl-2-cyanopyridine (390mg, 1.98mmol), bis(pinacolato)diboron (754mg, 2.97mmol), potassium acetate (493mg, 5.03mmol) and [1,1'-bis(diphenylphosphino)ferrocene] palladium(II) chloride dichlormethane complex(20mg). The resulting mixture was reacted under stirring in a nitrogen-protecting atmosphere at 90°C for 12hrs. The reaction mixture was cooled and directly used in the next step without a further treatment.

3. Preparation of 3-methyl-5-(9-oxo-10-(3-(trifluoromethyl)phenyl)-9,10-dihydropyrido[3,2-c][1,5 ]naphthyridin-2-yl)-2-cyanopyridine



[0224] 



[0225] To the cooled reaction liquor of 3-methyl-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-2-cyanopyridine obtained in the above step, was added 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne(500mg, 1.33mmol), palladium tetrakis(triphenylphosphine) (15mg) and 2N sodium carbonate solution (3.0mL). This system was reacted in a nitrogen-protecting atmosphere at 90 °C for 16hrs. The reaction mixture was cooled to room temperature and filtered. The organic layer was separated out, concentrated in a reduced pressure, dissolved in dichlormethane, sucessively washed with water and saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified with a silica-gel column chromatography (ethyl acetate) to produce 192 mg of the target compound in a yield of 31.6%.
Formula: C25H14F3N5O MW: 457.12 MS(M+H): 458.1
1H-NMR(d6-DMSO, 400 MHz):δ 9.30 (1H, s), 8.58 (1H, d), 8.40 (1H, d), 8.38 (1H, d), 8.20 (1H, d), 7.87 (1H, d), 7.82-7.72 (4H, m), 7.02 (1H, d), 2.52 (3H, s).

Example 29: Preparation of 5-(9-oxo-10-(3-(trifluoromethyl)phenyl)-9,10-dihydropyrido[3,2-c][1,5]naphthyr idin-2-yl)-3-cyanopyridine (Compound 35)



[0226] 

2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne (200mg, 0.532mmol) and 5-cyanopyridin-3-yl boric acid (95mg, 0.642mmol) were dissolved in 1,4-dioxane (12mL). To this system were added palladium tetrakis(triphenylphosphine) (10mg), and 2N sodium carbonate solution (0.9mL). The resulting mixture was reacted under reflux in a nitrogen-protecting atmosphere for 6hr. The reaction mixture was cooled to room temperature and filtered. The organic layer was separated out, concentrated in a reduced pressure, dissolved in dichlormethane, sucessively washed with water and saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified with a silica-gel column chromatography (ethyl acetate) to produce 186 mg of the target compound in a yield of 78.8%.
Formula: C24H12F3N5O MW: 443.10 MS(M+H): 444.10
1H-NMR(d6-DMSO, 400 MHz):δ 9.29 (1H, s), 9.05 (1H, d), 8.70 (1H, d), 8.57 (1H, d), 8.44 (1H, d), 8.39 (1H, d), 7.89-7.74 (5H, m), 7.02 (1H, d).

Example 30: Preparation of 2-(6-(pyrrolidin-1-yl)pyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1 ,5]naphthyridin-9(10H)-one (Compound 36)



[0227] 



[0228] The specific processure was the same as those in Example 1, Step 4, except for substituting 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne(188mg, 0.50mmol) and 2-(pyrrolidin-yl)-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-pyridine (165mg, 0.602mmol) respectively for 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne and 5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine to produce 198 mg of the target compound in a yield of 81.2%.
Formula: C27H20F3N5O MW: 487.16 MS(M+H): 488.2
1H-NMR(d6-DMSO, 400 MHz):δ 9.13 (1H, s), 8.36-8.33 (2H, m), 8.32 (1H, s), 8.19 (1H, d), 7.90-7.83 (2H, m), 7.71 (1H, t), 7.59 (1H, d), 6.95 (1H, d), 6.85 (1H, dd), 6.21 (1H, d), 3.46-3.38 (4H, m), 1.99-1.93 (4H, m).

Example 31: Preparation of 2-(1-methyl-1H-pyrazol-4-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]n aphthyridin-9(10H)-one (Compound 37)



[0229] 



[0230] The specific processure was the same as those in Example 1, Step 4, except for substituting 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne(188mg, 0.50mmol) and 1-methyl-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-pyrazole (125mg, 0.60mmol) respectively for 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne and 5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine to produce 163 mg of the target compound in a yield of 77.4%. Formula: C22H14F3N5O MW: 421.12 MS(M+H): 422.2 1H-NMR(d6-DMSO, 400 MHz):δ 9.12 (1H, s), 8.36-8.28 (2H, m), 7.97-7.89 (2H, m), 7.84 (1H, s), 7.76 (1H, t), 7.60 (1H, d), 7.43 (1H, s), 7.25 (1H, s), 6.94 (1H, d), 3.79 (3H, s).

Example 32: Preparation of 2-(6-(methylthio)pyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]n aphthyridin-9(10H)-one (Compound 38)



[0231] 


1. Preparation of 2-(methylthio)-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridine



[0232] 



[0233] To 1,4-dioxane (20mL) was successively added 5-bromo-2-(methylthio)pyridine (326mg, 1.60mmol), bis(pinacolato)diboron (608mg, 2.39mmol), potassium acetate (402mg, 4.08mmol) and [1,1'-bis(diphenylphosphino)ferrocene] palladium(II) chloride dichlormethane complex(35mg), The resulting mixture was reacted under stirring in a nitrogen-protecting atmosphere at 90°C for 12hrs. The reaction mixture was cooled and directly used in the next step without a further treatment.

2. Preparation of 2-(6-(methylthio)pyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]n aphthyridin-9(10H)-one



[0234] 



[0235] To the cooled reaction liquor of 2-(methylthio)-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridine obtained in the above step, were added 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne(300mg, 0.799mmol), palladium tetrakis(triphenylphosphine)(15mg) and 2N sodium carbonate solution(2.4mL). This system was reacted in a nitrogen-protecting atmosphere at 90 °C for 16h, cooled to room temperature and filtered. The organic layer was separated out, concentrated in a reduced pressure, dissolved in dichlormethane, sucessively washed with water and saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified with a silica-gel column chromatography (ethyl acetate) to produce 153 mg of the target compound in a yield of 41.3%.
Formula: C24H15F3N4OS MW: 464.09 MS(M+H): 465.1
1H-NMR (d6-DMSO, 400 MHz):δ 9.24 (1H, s), 8.50 (1H, d), 8.48 (1H, d), 8.37 (1H, d), 8.34 (1H, d), 7.90 (1H, d), 7.87 (1H, s), 7.74 (1H, t), 7.64 (1H, d), 7.18-7.09 (2H, m), 6.99 (1H, d), 2.56 (3H, s).

Example 33: Preparation of 2-(1H-pyrrolo[2,3-b]pyridin-5-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1 ,5]naphthyridin-9(10H)-one (Compound 39)



[0236] 



[0237] The specific processure was the same as those in Example 1, Step 4, except for substituting 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne(150mg, 0.399mmol) and 5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-pyrrolo[2,3-b]pyridine (117mg, 0.479mmol) respectively for 2-chloro-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-o ne and 5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine to produce 94 mg of the target compound in a yield of 51.6%.
Formula: C25H14F3N5O MW: 457.12 MS(M+H): 458.1
1H-NMR(d6-DMSO, 400 MHz):δ 11.83 (1H, s), 9.21 (1H, s), 8.45 (1H, d), 8.38 (1H, d), 8.36 (1H, d), 8.26 (1H, d), 7.90 (1H, d), 7.87 (1H, s), 7.78 (1H, t), 7.70 (1H, d), 7.53 (1H, d), 7.52 (1H, t), 6.98 (1H, d), 6.51 (1H, dd).

II. In vitro Enzymology and Antineoplastic Activity Assays of the present compounds



[0238] Hereinafter, the beneficial effects of the present compounds will be illustrated by in vitro enzymology and antineoplastic assay of the present compounds. However, it should be noted that the beneficial effects of the present compounds are not limited to the effects as illustrated below.

Assay 1



[0239] In vitro enzymology inhibitory activity of the present compounds

Samples:



[0240] The present compounds: lab-made, their chemical names and structural formulae are shown in the preparation examples.
mTOR enzymology Assay procedures

1. The agent's final concentrations and the compound solution preparation



[0241] 

1.1 mTOR(2.5nM) kinase solution, substrate ULight-4E-BP1 peptide 50 nM, ATP 10.8 uM;

1.2 4 folds kinase solution, 2 folds substrate and ATP solution;

1.3 test compound 1mM stock solution (final concentration 100 folds of DMSO solution).


2. Assay process



[0242] 

2.1 test compound 1 mM, diluted with DMSO 4 folds gradient, and then diluted with kinase buffer 25 folds;

2.2 To each well in a 384-well plate was added 2.5 µL series diluted compounds;

2.3 To each well was added 2.5 µL 4 fold kinase solution;

2.4 To each well was added 2.5 µL substrate /ATP solution;

2.5 incubated for 60 mins;

2.6 Envision data reading Lance signal (665 nM).


3. Data processing



[0243] The inhibition ratio% = (Lance signal-min)/(max-min)×100
wherein "max" is the DMSO control containing the enzyme but not containing the compound; "min" is the control without the kinase.

[0244] Input the data into GraphPad Prism5.0 to plot a curve and obtain IC50.

[0245] PI3Kα enzymology Assay procedures

1. The agent's final concentrations and the compound formulation



[0246] 

1.1 PI3Kα (1.65nM) kinase solution, substrate PIP2 50µM, ATP 25µM;

1.2 4 folds kinase solution, 2 folds substrate and ATP solution;

1.3 test compound 1mM stock solution (final concentration 100 folds of DMSO solution).


2. Assay process



[0247] 

2.1 test compound 1 mM, diluted with DMSO 4 folds gradient, and then diluted with kinase buffer 25 folds;

2.2 To each well in a 384-well plate was added 2.5 µL series diluted compounds;

2.3 To each well was added 2.5 µL 4 folds kinase solution;

2.4 To each well was added 2.5 µL substrate /ATP solution ;

2.5 incubated for 60 mins;

2.6 Envision data reading: Lance signal (665 nM).


3. Data processing



[0248] The inhibition ratio% = (sample RLU - min)/(max-min)×100
wherein "max" is the control without the kinase; "min" is the DMSO control containing the enzyme but not containing the compound.

[0249] Data were input into GraphPad Prism 5.0 to plot a curve and obtain IC50.

[0250] Results

[0251] See the Table 1 below.
Table 1 the activities of the present compounds in vitro enzymology (IC50)
Tested substance PI3Kα (nM) mTOR (nM)
Compound 1 3.2 0.87
Compound 13 2.4 0.47
Compound 14 8.8 4.4
Compound 15 6.1 3
Compound 16 58 8.7
Compound 17 45 5.3
Compound 18 3.6 2.6
Compound 19 158 2.3
Compound 20 104 6
Compound 38 17 5
Compound 39 2.5 0.89
Conclusion    


[0252] It could be seen from Table 1 that the present compounds had a good in vitro inhibitory activity for both of the enzymes PI3Kα and mTOR.

Assay 2



[0253] In vitro cellular inhibitory activity of the present compounds

Samples:



[0254] Controls: Torin-2, its structure is shown in the section "Background" hereinbefore and synthesized according to the method in the following literature: Journal of Medicinal Chemistry (2011), 54(5), 1473-1480, "Discovery of 9-(6-Aminopyridin-3-yl)-1-(3-(trifluoromethyl)phenyl)benzo[h][1,6]naphthyridin-2(1H)-one (Torin2) as a potent, selective, and orally available mammalian target of rapamycin (mTOR) inhibitor for treatment of cancer"; and
the present compounds: lab-made, their chemical names and structural formulae are shown in the preparation examples.

Positive control: paclitaxel



[0255] Assay procedures:
  1. 1. Formulating the agents and the compounds: Formulating the PBS, the XTT working liquor, the paclitaxel stock solution and gradient dilute solutions thereof, and the stock solution of test compound and gradient dilute solutions of test compounds.
  2. 2. Culturing cells: Thawing cells, Passing cells, Freezing and preserving cells.
  3. 3. Plating Cells:

    Praparing the cell suspension;

    The cell suspension was added to a 96-well plate, 100 µL per well;

    The plate was placed in the incubator and incubated at 37°C under 5% CO2 overnight.

  4. 4. Treating with drugs
    Drugs were added to the cell culture plate. The plate was placed in the CO2 incubator and incubated for 72 hours.
  5. 5. Testing the cell viability with the XTT method
    The XTT working solution was added to the plate. The plate was placed in the CO2 incubator for 2 hours. Then the plate was placed in a microplate reader to read the absorbance at 450 nm.
  6. 6. Data processing
    1. 1) %inhibition=(Absorbance(Vehicle)-Absorbance(Compound))/(Absorbance(Vehicle) - Absorbance(positive control)×100%;
    2. 2) Data were input into GraphPad Prism 5.0 to plot a curve and obtain IC50. Result:

      See the Table 2 below.

Table 2: In vitro cytological activities of the present compounds (IC50)
Tested substance U87MG cells (nM) A549 cells (nM)
Torin-2 12.4 14.7
Compound 1 8.9 11.9
Compound 7 29.3 -
Compound 13 26.4 -
Compound 15 12.7 20.2
Compound 39 8.4 6.8

Conclusion:



[0256] It could be seen from Table 2 that the present compounds could effectively inhibit the proliferations of U87MG and A549 cells, and had a comparable activity over the control drug Torin-2.

Assay 3:



[0257] Pharmacokinetic assay of the present compounds in rat in vivo

Tested animals



[0258] Male SD rats, each test substance, each administration, three rats were used, having a body weight of 200-250 g.

Test substances:



[0259] Control drug: Torin-2, dissolved with 5%NMP+40%PEG400+55% sterile water for injection;
the present compounds: Compound 16, dissolved with 5%NMP+60%PEG400+35% sterile water for injection, other compounds, dissolved with 5%NMP+ 40%PEG400+55% sterile water for injection.

Assay procedures:



[0260] Administration: The administration manner and dosage of the test substance are shown in the following Table 3
Table 3: Administration manner and dosage of Test substances
Tested substance Intervenous push injection (IV) intragastric administration (PO)
Dosage (mg/kg) Volume (mL/kg) Dosage (mg/kg) Volume (mL/kg)
Torin-2 2 4 4 8
Compound 1 2 2 4 4
Compound 13 2 2 4 4
Compound 14 - - 4 4
Compound 15 2 2 4 4
Compound 16 0.45 2 4 4
Compound 17 2 2 4 4


[0261] Blood collection: each of about 100 µL whole blood were collected at 0 h before administration (pre-administration), and at 0.083 h, 0.25 h, 0.5 h, 1 h, 2 h, 4 h, 6 h, 8 h and 24 h after administration. The collected blood samples were centrifuged at 8000rpm in a high-speed centrifuge for 6 mins to separate the blood plasm. The separated plasm was freezing-preserved at -80 °C in a refrigerator.

[0262] Plasm sample analysis: The plasm sample was treated by a liquid-liquid extraction. 20µL of the plasm was taken, subjected to a vortex at 1500 rpm for 10 mins, and then centrifuged at 12000 rpm for 5 mins. 400µL of the supernatant was taken and blowed to dryness in the nitrogen gas. The resulting substance is re-dissolved in 200µL methanol:water (1:1, V/V), and the solution was analyzed with LC-MS/MS.

Result:



[0263] See the Table 4 below.
Table 4: Rat PK evaluation result of the present compounds (Dosage in Table 3)
Tested substance I V(2mg/kg) PO(4mg/kg)
T1/2 (h) AUClast (h·ng/ml) T1/2 (h) AUClast (h·ng/ml) F(%)
Torin-2 0.86 259.24 2.55 104.32 22.64
Compound 1 1.79 1306 2.78 1029 43.7
Compound 13 0.62 710.62 1.44 706.62 48.96
Compound 14 - - 3.49 445.1 42.26
Compound 15 0.71 440.83 2.36 549.8 64.96
Compound 16 1.52 194.82 1.67 409.84 23.04
Compound 17 0.61 723.12 5.68 212.35 20.82
T1/2 represents the half-life
AUClast represents the area under curve on administration from time=0→t)
F% represents the absolute bioavailability

Conclusion



[0264] It could be seen from Table 4 that, whether with the intravenous injection adminstration or with the oral administration, the present compounds had a remarkably higher AUClast than that of the control drug, which demonstrated that the present compounds had a good pharmacokinetic characteristic and an excellent potential of being developed into drug.


Claims

1. A compound represented by general formula (I), and its pharmaceutically acceptable salt, stereoisomer or deuteride thereof:

wherein:

X is O;

R1 is hydrogen or the following group that is unsubstituted or substituted by 1-3 R8: C1-6alkyl, C2-6alkenyl, C2-6alkynyl, 3-14-membered cycloalkyl, 6-14-membered aryl, 3-14-membered heterocyclyl, 7-12-membered spiro ring group or 7-12-membered bridged ring group;

R2 is hydrogen or the following group that is unsubstituted or substituted by 1-3 R8': C1-6alkyl, C2-6alkenyl, C2-6alkynyl, 3-14-membered cycloalkyl, 6-14-membered aryl, 3-14-membered heterocyclyl, 7-12-membered spiro ring group or 7-12-membered bridged ring group;

each of R3 and R4 is independently selected from the group consisting of hydrogen, halogen, cyano, amino, hydroxy, sulfonyl, -SO2C1-6alkyl, and C1-6alkyl and C1-6alkoxyl that are unsubstituted or substituted with 1-3 substituents selected from halogen, hydroxy and/or carboxyl;

R5 is selected from the group consisting of hydrogen, cyano, amino, sulfonyl, -SO2C1-6alkyl, and C1-6alkyl and C1-6alkoxyl that are unsubstituted or substituted with 1-3 substituents selected from halogen, hydroxy and/or carboxyl;

each of R6 and R7 is independently selected from the group consisting of hydrogen, hydroxy, halogen, amino, and C1-6alkyl and C1-6alkoxyl that are unsubstituted or substituted with 1-3 substituents selected from halogen, hydroxy and/or carboxyl;

each of R8 and R8' is independently selected from the group consisting of

(1) hydroxy, halogen, amino, cyano, -(CH2)nNRaRb, -(CH2)nC(O)Rc, -(CH2)nS(O)mRc, -(CH2)nS(O)mNRaRb, -(CH2)nNRaS(O)mRc, -(CH2)nC(O)(CH2)nNRaRb, -(CH2),OC(O)Rc, -(CH2)nNRaC(O)Rc, and -(CH2)nNRaC(O)NRaRb,

(2) C1-6alkyl, C2-6alkenyl, C2-6alkynyl and C1-6alkoxyl, all of which are unsubstituted or substituted by 1-3 substitutents selected from cyano, halogen and/or hydroxy, and

(3) 3-14-membered cycloalkyl, 6-14-membered aryl and 3-14-membered heterocyclyl, all of which are unsubstituted or substituted by 1-3 substitutents selected from cyano, trifluoromethyl, halogen, C1-6alkyl, C2-8alkenyl, C2-8alkynyl, C1-6alkoxyl, -(CH2)nNRaRb, -(CH2)nC(O)Rc', -(CH2)nC(O)(CH2)nNRaRb, -(CH2)nS(O)mRc', -(CH2)nS(O)mNRaRb, -(CH2)nNRaS(O)mRc', -(CH2)nOC(O)Rc', -(CH2)nNRaC(O)Rc' and/or -(CH2)nNRaC(O)NRaRb;

wherein

each of Ra and Rb is independently selected from the group consisting of hydrogen, or C1-6alkyl that is unsubstituted or substituted by 1-3 substitutents selected from hydroxy, halogen and/or cyano;

Rc is C1-6alkyl or C1-6alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from hydroxy, halogen, cyano and/or trifluoromethyl; Rc' is C1-6alkyl, C1-6alkoxyl, 3-8-membered monocyclic cycloalkyl or 3-8-membered monocyclic heterocyclyl, all of which are unsubstituted or substituted by 1-3 substitutents selected from hydroxy, halogen and/or cyano;

m is 0, 1 or 2; and

n is 0-4.


 
2. The compound of claim 1, and its pharmaceutically acceptable salt, stereoisomer or deuteride thereof, wherein

R1 is 3-14-membered cycloalkyl, 6-14-membered aryl, 3-14-membered heterocyclyl, 7-12-membered spiro ring group or 7-12-membered bridged ring group, all of which are unsubstituted or substituted by 1-3 R8;

R2 is 3-14-membered cycloalkyl, 6-14-membered aryl or 3-14-membered heterocyclyl, all of which are unsubstituted or substituted by 1-3 R8';

each of R3 and R4 is independently selected from the group consisting of hydrogen, halogen, cyano, amino, hydroxy, trifluoromethyl and trifluoromethoxy;

R5 is hydrogen, cyano or amino;

each of R6 and R7 is independently selected from the group consisting of hydrogen, hydroxy, halogen, amino, and C1-6alkyl;

each of R8 and R8' is independently selected from the group consisting of

(1) hydroxy, halogen, amino, cyano, -(CH2)nC(O)Rc, -(CH2)nNRaRb, -(CH2)nS(O)mRc, -(CH2)nS(O)mNRaRb, -(CH2)nNRaS(O)mRc, -(CH2)nC(O)(CH2)nNRaRb, -(CH2),OC(O)Rc, -(CH2)nNRaC(O)Rc and -(CH2)nNRaC(O)NRaRb,

(2) C1-6alkyl and C1-6alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from cyano, halogen and/or hydroxy, and

(3) 3-8-membered monocyclic cycloalkyl, 6-14-membered aryl and 3-8-membered monocyclic heterocyclyl, all of which are unsubstituted or substituted by 1-3 substitutents selected from cyano, trifluoromethyl, halogen, C1-6alkyl, C1-6alkoxyl, -(CH2)nNRaRb, -(CH2)nC(O)Rc', -(CH2)nC(O)(CH2)nNRaRb, -(CH2)nS(O)mRc', -(CH2)nS(O)mNRaRb, -(CH2)nNRaS(O)mRc', -(CH2)nOC(O)Rc', -(CH2)nNRaC(O)Rc' and/or -(CH2)nNRaC(O)NRaRb;

wherein

each of Ra and Rb is independently selected from the group consisting of hydrogen, or C1-6alkyl that is unsubstituted or substituted by 1-3 substitutents selected from hydroxy, halogen and/or cyano;

Rc is C1-6alkyl or C1-6alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from hydroxy, halogen, cyano and/or trifluoromethyl; Rc' is C1-6alkyl or C1-6alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from hydroxy, halogen and/or cyano;

m is 0, 1 or 2; and

n is 0, 1, 2 or 3.


 
3. The compound of claim 1, and its pharmaceutically acceptable salt, stereoisomer or deuteride thereof, wherein

R1 is 3-8-membered monocyclic cycloalkyl, 6-14-membered aryl or 3-8-membered monocyclic heterocyclyl, all of which are unsubstituted or substituted by 1-3 R8;

R2 is 3-8-membered monocyclic cycloalkyl, 6-14-membered aryl or 5-10-membered heterocyclyl, all of which are unsubstituted or substituted by 1-3 R8';

each of R3, R4 and R5 is hydrogen;

each of R6 and R7 is independently hydrogen or C1-4alkyl;

R8 is selected from the group consisting of

(1) hydroxy, halogen, cyano, amino, and -(CH2)nC(O)Rc,

(2) C1-4alkyl and C1-4alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from cyano, halogen and/or hydroxy, and

(3) 5-8-membered saturated monocyclic heterocyclyl, which is unsubstituted or substituted by 1-3 substitutents selected from cyano, trifluoromethyl, halogen, -(CH2)nC(O)Rc', -(CH2)nC(O)(CH2)nNRaRb and/or -(CH2)nS(O)mRc';

R8' is selected from the group consisting of

(1) hydroxy, halogen, cyano, amino, -(CH2)nC(O)Rc, -(CH2)nNRaRb, -(CH2)nS(O)mRc, -(CH2)nS(O)mNRaRb, -(CH2)nNRaS(O)mRc, -(CH2)nC(O)(CH2)nNRaRb, -(CH2),OC(O)Rc, -(CH2)nNRaC(O)Rc and -(CH2)nNRaC(O)NRaRb,

(2) C1-4alkyl and C1-4alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from cyano, halogen and/or hydroxy, and

(3) 5-8-membered monocyclic heterocyclyl, which is unsubstituted or substituted by 1-3 substitutents selected from cyano, trifluoromethyl, halogen, C1-4alkyl, C1-4alkoxyl, -(CH2)nNRaRb, -(CH2)nC(O)Rc', -(CH2)nC(O)(CH2)nNRaRb, -(CH2)nS(O)mRc', -(CH2)nS(O)mNRaRb, -(CH2)nNRaS(O)mRc', -(CH2)nOC(O)Rc', -(CH2)nNRaC(O)Rc' and/or -(CH2)nNRaC(O)NRaRb;

each of Ra and Rb is independently hydrogen, or C1-4alkyl that is unsubstituted or substituted by 1-3 substitutents selected from hydroxy and/or halogen;

Rc is C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from hydroxy, halogen and/or cyano;

Rc' is C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from hydroxy and/or halogen;

m is 0, 1 or 2; and

n is 0, 1, 2 or 3.


 
4. The compound of claim 1, and its pharmaceutically acceptable salt, stereoisomer or deuteride thereof, wherein

R1 is 6-10-membered aryl, 3-8-membered saturated monocyclic heterocyclyl or

5-6-membered aromatic monocyclic heterocyclyl, all of which are unsubstituted or substituted by 1-3 R8;

R2 is 6-10-membered aryl or 5-10-membered heterocyclyl, both of which are unsubstituted or substituted by 1-3 R8';

each of R3, R4 and R5 is hydrogen;

each of R6 and R7 is independently hydrogen or C1-4alkyl;

R8 is

(1) hydroxy, halogen, cyano, amino or -C(O)Rc,

(2) C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from cyano, halogen and/or hydroxy, or

(3) 5-6-membered saturated monocyclic heterocyclyl, which is unsubstituted or substituted by 1-3 substitutents selected from cyano, trifluoromethyl, halogen, -C(O)Rc', -C(O)(CH2)nNRaRb and/or -S(O)2Rc';

R8' is

(1) hydroxy, halogen, cyano, amino, -(CH2)nNRaC(O)Rc or -(CH2)nS(O)mRc,

(2) C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from cyano, halogen and/or hydroxy, or

(3) 5-6-membered monocyclic heterocyclyl, which is unsubstituted or substituted by cyano, trifluoromethyl, halogen, C1-4alkyl or C1-4alkoxyl;

each of Ra and Rb is independently hydrogen, or C1-4alkyl that is unsubstituted or substituted by 1-3 substitutents selected from hydroxy and/or halogen;

Rc is C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from hydroxy, halogen and/or cyano;

Rc' is C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by 1-3 substitutents selected from hydroxy and/or halogen;

m is 0, 1 or 2; and

n is 0, 1, 2 or 3.


 
5. The compound of claim 4, and its pharmaceutically acceptable salt, stereoisomer or deuteride thereof, wherein

R1 is phenyl, naphthyl or 5-6-membered saturated monocyclic heterocyclyl, all of which are unsubstituted or substituted by 1-3 R8;

R2 is phenyl, pyridinyl, pyrimidinyl, pyridazinyl, pyrazinyl, pyrazolyl, imidazolyl, pyrrolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, indazolyl, quinolinyl, isoquinolinyl, indolyl, pyrrolopyridine, dihydropyrrolopyridine or pyrazolopyridinyl, all of which are unsubstituted or substituted by 1-3 R8';

each of R6 and R7 is independently hydrogen or methyl;

R8 is

(1) amino or -C(O)Rc, Rc is C1-4alkyl that is unsubstituted or substituted by hydroxy or halogen,

(2) C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by cyano or 1-3 fluoro, or

(3) piperazinyl or piperidinyl, both of which are unsubstituted or substituted by cyano or trifluoromethyl;

R8' is

(1) hydroxy, halogen, amino, cyano, -NHC(O)Rc or -S(O)mRc, wherein m is 0 or 2, Rc is C1-4alkyl,

(2) C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by hydroxy or 1-3 fluoro, or

(3) pyridinyl, piperidinyl, pyrimidinyl, pyridazinyl, pyrazinyl, piperazinyl, pyrazolyl, imidazolyl, pyrrolyl, pyrrolidinyl or morpholinyl, all of which are unsubstituted or substituted by cyano, trifluoromethyl, halogen, C1-4alkyl or C1-4alkoxyl.


 
6. The compound of claim 4, and its pharmaceutically acceptable salt, stereoisomer or deuteride thereof, wherein

R1 is 6-10-membered aryl or 5-6-membered monocyclic heterocyclyl, both of which are unsubstituted or substituted by 1-3 R8., wherein R8 is (1) amino or -C(O)Rc, Rc is C1-4alkyl that is unsubstituted or substituted by hydroxy or halogen, (2) C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by cyano or 1-3 halogens, or (3) 5-6-membered monocyclic heterocyclyl that is unsubstituted or substituted by cyano or trifluoromethyl;
and/or

R2 is 6-10-membered aryl, 5-6-membered partially saturated monocyclic heterocyclyl, 5-6-membered aromatic monocyclic heterocyclyl or 9-10-membered fused heterocyclyl, all of which are unsubstituted or substituted by 1-3 R8', wherein R8' is (1) hydroxy, halogen, amino, cyano, -NHC(O)Rc or -S(O)mRc, wherein m is 0, 1 or 2, Rc is C1-4alkyl, (2) C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by hydroxy or 1-3 halogens, or (3) 5-6-membered monocyclic heterocyclyl, which is unsubstituted or substituted by cyano, trifluoromethyl, halogen, C1-4alkyl or C1-4alkoxyl.


 
7. The compound of claim 5, and its pharmaceutically acceptable salt, stereoisomer or deuteride thereof, wherein

R1 is phenyl, piperidinyl or piperazinyl, all of which are unsubstituted or substituted by 1-2 R8;

R2 is phenyl, pyridinyl, pyrimidinyl, pyridazinyl, pyrazinyl, pyrazolyl, imidazolyl, pyrrolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, quinolinyl, isoquinolinyl, pyrrolopyridine, dihydropyrrolopyridine or indolyl, all of which are unsubstituted or substituted by 1-2 R8';

R8 is

(1) amino or -C(O)Rc, Rc is C1-4alkyl that is unsubstituted or substituted by hydroxy,

(2) C1-4alkyl that is unsubstituted or substituted by cyano or three fluoro, or

(3) piperazinyl or piperidinyl, both of which are unsubstituted or substituted by cyano or trifluoromethyl;

R8' is

(1) halogen, amino, cyano, -NHC(O)Rc or -S(O)mRc, wherein m is 0 or 2, Rc is C1-4alkyl,

(2) C1-4alkyl or C1-4alkoxyl, both of which are unsubstituted or substituted by hydroxy or three fluoro, or

(3) piperazinyl, pyrazolyl, piperidinyl, morpholinyl, pyrimidinyl, pyridazinyl, pyrazinyl, pyrrolyl or pyrrolidinyl, all of which are unsubstituted or substituted by C1-4alkyl.


 
8. The compound of claim 1, and its pharmaceutically acceptable salt, stereoisomer or deuteride thereof, wherein said compound is selected from:

2-((6-aminopyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphth yridin-9(10H)-one,

2-((6-methoxypyridin-3-yl)-10-(4-(piperazin-1-yl)-3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

(R)-2-(6-aminopyridin-3--yl)-10-(1-(2-hydroxypropanoyl)piperidin-4-yl)pyrido[3, 2-c][1,5]naphthyridin-9(10H)-one,

(R)-10-(1-(2-hydroxypropanoyl)piperidin-4-yl)-2-(6-methoxypyridin-3-yl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

2-methyl-2-(4-(9-oxo-2-(quinolin-3-yl)pyrido[3,2-c][1,5]naphthyridin-10(9H)-yl)phenyl)propanenitrile,

2-((6-aminopyridin-3-yl)-10-(4-(piperazin-1-yl)-3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

2-((4-(2-(6-aminopyridin-3-yl)-9-oxopyrido[3,2-c][1,5]naphthyridin-10(9H)-yl)phenyl)-2-methylpropanenitrile,

2-((6-methoxypyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

2-((quinolin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

10-((4-(piperazin-1-yl)-3-(trifluoromethyl)phenyl)-2-(quinolin-3-yl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

(R)-10-(1-(2-hydroxypropanoyl)piperidin-4-yl)-2-(quinolin-3-yl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

2-((4-(2-(6-methoxypyridin-3-yl)-9-oxopyrido[3,2-c][1,5]naphthyridin-10(9H)-yl)phenyl)-2-methylpropanenitrile,

2-((2-aminopyrimidin-5-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

2-((2-methoxypyrimidin-5-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

N-(5-(9-oxo-10-(3-(trifluoromethyl)phenyl)-9,10-dihydropyrido[3,2-c][1,5]naphthyridin-2-yl)pyridin-2-yl)acetamide,

5-((9-oxo-10-(3-(trifluoromethyl)phenyl)-9,10-dihydropyrido[3,2-c][1,5]naphthyridin-2-yl)-2-cyanopyridine,

2-((1H-pyrrolo[2,3-b]pyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

2-((6-(hydroxymethyl)pyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

2-((6-(4-methylpiperazin-1-yl)pyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

2-((6-(1H-pyrazol-1-yl)pyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

2-((4-(2-(2-methoxypyrimidin-5-yl)-9-oxopyrido[3,2-c][1,5]naphthyridin-10(9H)-yl)phenyl)-2-methylpropanenitrile,

2-((6-(methylsulfonyl)pyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

2-((6-methoxypyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

2-((6-morpholinopyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

2-((5-methoxypyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

2-((2-methoxypyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

10-((3-(trifluoromethyl)phenyl)-2-(6-(trifluoromethyl)pyridin-3-yl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

2-((6-methylpyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

2-((3,5-dimethylisoxazol-4-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

2-((5-fluoropyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

10-((3-(trifluoromethyl)phenyl-2-(5-(trifluoromethyl)pyridin-3-yl)pyrido[3,2-c][1 ,5]naphthyridin-9(10H)-one,

2-((1-methyl-1H-pyrazol-5-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

2-((thiazol-5-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

3-methyl-5-(9-oxo-10-(3 -(trifluoromethyl)phenyl)-9,10-dihydropyrido[3,2-c][1,5]naphthyridin-2-yl)-2-cyanopyridine,

5-((9-oxo-10-(3-(trifluoromethyl)phenyl)-9,10-dihydropyrido[3,2-c][1,5]naphthyridin-2-yl)-3-cyanopyridine,

2-((6-(pyrrolidin-1-yl)pyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

2-((1-methyl-1H-pyrazol-4-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

2-((6-(methylthio)pyridin-3-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

2-((1H-pyrrolo[2,3-b]pyridin-5-yl)-10-(3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

2-((6-(methylsulfonyl)pyridin-3-yl)-10-(4-(piperazin-1-yl)-3 -(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

(R)-10-(1-(2-hydroxypropanoyl)piperidin-4-yl)-2-(6-(methylsulfonyl)pyridin-3-yl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

2-methyl-2-(4-(2-(6-(methylsulfonyl)pyridin-3-yl)-9-oxopyrido[3,2-c][1,5]naphthyridin-10(9H)-yl)phenyl)propanenitrile,

N-(5-(9-oxo-10-(4-(piperazin-1-yl)-3-(trifluoromethyl)phenyl)-9,10-dihydropyrido[3,2-c][1,5]naphthyridin-2-yl)pyridin-2-yl)acetamide,

(R)-N-(5-(10-(1-(2-hydroxypropanoyl)piperidin-4-yl)-9-oxo-9,10-dihydropyrido[3,2-c][1,5]naphthyridin-2-yl)pyridin-2-yl)acetamide,

N-(5-(10-(4-(2-cyanopropan-2-yl)phenyl)-9-oxo-9,10-dihydropyrido[3,2-c][1,5]naphthyridin-2-yl)pyridin-2-yl)acetamide,

5-((9-oxo-10-(4-(piperazin-1-yl)-3-(trifluoromethyl)phenyl)-9,10-dihydropyrido[3,2-c][1,5]naphthyridin-2-yl)-2-cyanopyridine,

(R)-5-(10-(1-(2-hydroxypropanoyl)piperidin-4-yl)-9-oxo-9,10-dihydropyrido[3,2-c][1,5]naphthyridin-2-yl)-2-cyanopyridine,

5-((10-(4-(2-cyanopropan-2-yl)phenyl)-9-oxo-9,10-dihydropyrido[3,2-c][1,5]naphthyridin-2-yl)-2-cyanopyridine,

2-((6-(methylthio)pyridin-3-yl)-10-(4-(piperazin-1-yl)-3-(trifluoromethyl)phenyl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one,

(R)-10-(1-(2-hydroxypropanoyl)piperidin-4-yl)-2-(6-(methylthio)pyridin-3-yl)pyrido[3,2-c][1,5]naphthyridin-9(10H)-one, and

2-methyl-2-(4-(2-(6-(methylthio)pyridin-3-yl)-9-oxopyrido[3,2-c][1,5]naphthyridin-10(9H)-yl)phenyl)propanenitrile.


 
9. A pharmaceutical composition, which contains the compound according to any one of claims 1-8, and its pharmaceutically acceptable salt, stereoisomer or deuteride thereof.
 
10. The pharmaceutical composition of claim 9, which further comprises one or more antineoplastic agent(s) and/or immunosuppressive agent(s), said antineoplastic agent and said immunosuppressive agent are antimetabolite selected from capecitabine, gemcitabine, pemetrexed disodium; growth factor inhibitor selected from pazopanib, imatinib, erlotinib, lapatinib, gefitinib, vandetanib; antibody selected from herceptin, bevacizumab; mitotic inhibitor selected from paclitaxel, vinorelbine, docetaxel, doxorubicin; antineoplastic hormone selected from letrozole, tamoxifen, fulvestrant, flutamide, triptorelin; alkylating agent selected from cyclophosphamide, chlormethine, melphalan, chlorambucil, carmustine; metallic platinum selected from carboplatin, cisplatin, oxaliplatin; topoismerase inhibitor selected from topotecan camptothecin, topotecan, irinotecanpto; immunosuppressive agent selected from everolimus, sirolimus, torisel; purine analogues selected from 6-mercaptopurine, 6-thioguanine, azathioprine; antibiotics selected from actinomycin D, daunorubicin, adriamycin, mitoxantrone, bleomycin, plicamycin; platinum complex selected from cisplatin, carboplatin; adrenal cortex inhibitors selected from aminoglutethimide.
 
11. A pharmaceutical formulation containing the compound according to any one of claims 1-8, and its pharmaceutically acceptable salt, stereoisomer or deuteride thereof and one or more pharmaceutically acceptable carriers, which is in any dosage form that is clinically or pharmaceutically acceptable.
 
12. A use of the compound according to any one of claims 1-8, and its pharmaceutically acceptable salt, stereoisomer or deuteride thereof in the manufacture of a medicament for treating and/or preventing a proliferative disease.
 
13. A method for treating a proliferative disease, which comprises a step of administering an effective amount of the compound of general formula (I) according to any one of claims 1-8, and its pharmaceutically acceptable salt, stereoisomer or deuteride thereof, or a pharmaceutical composition containing the compound of general formula (I), and its pharmaceutically acceptable salt, stereoisomer or deuteride thereof to a subject in need thereof.
 
14. The use of claim 12 or the method of claim 13, wherein the proliferative disease is a cancer or a non-carcinomatous proliferative disease, said cancer is selected from cerebroma, lung cancer, non-small cell lung cancer, squamous epithelial cell carcinoma, bladder carcinoma, gastric cancer, ovarian cancer, peritoneal cancer, pancreatic cancer, mammary cancer, head and neck cancer, cervical cancer, endometrial cancer, colorectal cancer, liver cancer, renal carcinoma, adenocarcinoma of esophagus, esophageal squamous cell cancer, solid tumor, non-Hodgkin lymphoma, glioma, glioblastoma multiforme, glioma sarcomatosum, prostate carcinoma, thyroid carcinoma, female genital tract cancer, carcinoma in situ, lymphoma, histiocytic lymphoma, neurofibromatosis, osteocarcinoma, cutaneous carcinoma, brain cancer, colon carcinoma, testis carcinoma, small cell lung cancer, gastrointestinal stromal tumor, prostate tumor, mast cell tumor, multiple myeloma, melanoma, neurogliocytoma, glioblastoma, astrocytoma, neuroblastoma, sarcoma; said non-carcinomatous proliferative disease is selected from skin or prostate benign proliferation.
 
15. A method for preparing the compound of general formula (I) of claim 1, i.e., the following formula

which method comprises the following steps:

wherein R1, R2, R3, R4, R5, R6 and R7 are defined as hereinbefore, Hal1, Hal2 and Hal3 represent halogen, which is each independently selected from the group consisting of F, Cl, Br and I, and Hal1, Hal2 and Hal3 can be identical or different; Alk represents a lower alkyl; "anhydride" is an organic acid anhydride;

(1) preparation of intermediate 1
starting material 1 and starting material 2 are reacted under heating to reflux in an alcohol organic solvent in presence of a base until the starting material disappears to produce intermediate 1;

(2) preparation of intermediate 2'
intermediate 1 is reacted with an reductant in an alcohol organic solvent; the solvent is removed under a reduced pressure; water is added to the reaction mixture; the resulting mixture is extracted with a halogenated hydrocarbon organic solvent; the organic phase is concentrated, to which is added an oxidant; the resulting mixture is reacted under stirring to produce intermediate 2';

(3) preparation of intermediate 2
in the nitrogen protection, intermediate 2' and a Grignard reagent R6-Mg-Hal3 are reacted and then oxidized to produce Intermediate 2;

(4) preparation of intermediate 3
method 1: in a sealed vessel, intermediate 2 and starting material 3 are reacted in an alcohol organic solvent in the presence of an inorganic base at 110-180°C to produce intermediate 3; or
method 2: intermediate 2 is dissolved in a non-protonic polar organic solvent and starting material 3'; the reaction is conducted in a microwave reactor until the starting material disappears to produce intermediate 3;
and

(5) preparation of compound of formula (I)
intermediate 3 and starting material 4 are dissolved in an organic solvent; the resulting mixture was reacted in the presence of a catalyst and a base under reflux in a nitrogen-protecting atmosphere to produce the compound of formula (I);
if necessary, a functional group that needs to be protected can be protected; and afterwards can be deprotected according to the conventional method.


 





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Cited references

REFERENCES CITED IN THE DESCRIPTION



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Patent documents cited in the description




Non-patent literature cited in the description