Methods for treating a suspension of mononuclear cells to facilitate extracorporeal photopheresis

(19)

(11)

EP 2 813 253 A3

(12)	EUROPEAN PATENT APPLICATION

(88)	Date of publication A3:
	18.03.2015 Bulletin 2015/12

(43)	Date of publication A2:
	17.12.2014 Bulletin 2014/51

(21)	Application number: 13188736.6

(22)	Date of filing: 15.10.2013

(51)

International Patent Classification (IPC):

A61M 1/36^(2006.01)

A61M 1/34^(2006.01)

(84)	Designated Contracting States:
	AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
	Designated Extension States:
	BA ME

(30)

Priority:

13.06.2013 US 201313916854

(71)	Applicant: Fenwal, Inc.
	Lake Zurich, IL 60047 (US)

(72)	Inventor:
	Radwanski, Katherine Des Plaines, IL Illinois 60016 (US)

(74)	Representative: Prins, Hendrik Willem et al
	Arnold & Siedsma Bezuidenhoutseweg 57 2594 AC The Hague 2594 AC The Hague (NL)

(54)	Methods for treating a suspension of mononuclear cells to facilitate extracorporeal photopheresis

(57) A method for performing a photopheresis procedure is provided comprising collecting MNCs in a suspension comprising RBCs and plasma and lysing the red blood cells in the solution, preferably by combining the suspension with a solution to cause lysis. In one example, the solution for causing lysis of the red blood cells comprises ammonium chloride, and the suspension including the ammonium chloride is incubated to cause lysing. After lysing, the suspension may be washed to remove plasma and hemoglobin freed by the lysis of the red blood cells, and an ultraviolet light activated substance is added to the suspension. The suspension is then irradiated with ultraviolet light.

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