(57) The invention relates to a process for making an oligo(alkylene glycol) functionalized
co-polyisocyanopeptide, wherein the process comprises the steps of:
i) copolymerizing
- a first comonomer of an oligo(alkylene glycol) functionalized isocyanopeptide grafted
with a linking group and
- a second comonomer of a non-grafted oligo(alkylene glycol) functionalized isocyanopeptide,
wherein the molar ratio between the first comonomer and the second comonomer is 1:500
and 1:30 and
ii) adding a reactant of a spacer unit and a cell adhesion factor to the copolymer
obtained by step i), wherein the spacer unit is represented by general formula A-L-B,
wherein the linking group and group A are chosen to react and form a first coupling
and the cell adhesion factor and group B are chosen to react and form a second coupling,
wherein the first coupling and the second coupling are independently selected from
the group consisting of alkyne-azide coupling, dibenzocyclooctyne-azide coupling,
oxanorbornadiene-based-azide couplings, vinylsulphone-thiol coupling, maleimide-thiol
coupling, methyl methacrylate-thiol coupling, ether coupling, thioether coupling,
biotin-strepavidin coupling, amine-carboxylic acid resulting in amides linkages, alcohol-carboxylic
acid coupling resulting in esters linkages and NHS-Ester (N-Hydroxysuccinimide ester)-amine
coupling and
wherein group L is a linear chain segment having 10-60 bonds between atoms selected
from C, N, O and S in the main chain.
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