Immobilisation of chelating groups for immobilised metal ion chromatography

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EP 2 826 555 A3

(12)	EUROPEAN PATENT APPLICATION

(88)	Date of publication A3:
	27.01.2016 Bulletin 2016/04

(43)	Date of publication A2:
	21.01.2015 Bulletin 2015/04

(21)	Application number: 14173856.7

(22)	Date of filing: 06.08.2008

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International Patent Classification (IPC):

B01J 20/32^(2006.01)
B01D 15/38^(2006.01)
C07K 14/00^(2006.01)

B01J 45/00^(2006.01)
C07K 1/22^(2006.01)
C07K 1/13^(2006.01)

(84)	Designated Contracting States:
	AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MT NL NO PL PT RO SE SI SK TR

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Priority:

06.08.2007 EP 07015389
06.08.2007 US 954144 P
14.07.2008 EP 08012686

(62)	Application number of the earlier application in accordance with Art. 76 EPC:
	08785398.2 / 2183049

(71)	Applicant: Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.
	80539 München (DE)

(72)	Inventors:
	Frey, Steffen 37075 Göttingen-Herberhausen (DE) Görlich, Dirk 37077 Göttingen (DE)

(74)	Representative: Weickmann & Weickmann
	Postfach 860 820 81635 München 81635 München (DE)

(54)	Immobilisation of chelating groups for immobilised metal ion chromatography

(57) The present invention refers to a method for binding a polycarboxylic acid to a solid phase. Further, the invention refers to a solid phase having a polycarboxylic acid immobilized thereto and methods of using the solid phase, e.g. for purifying Histagged recombinant polypeptides.

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