(19)
(11) EP 2 875 128 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
24.06.2020 Bulletin 2020/26

(45) Mention of the grant of the patent:
20.05.2020 Bulletin 2020/21

(21) Application number: 13740348.1

(22) Date of filing: 18.07.2013
(51) International Patent Classification (IPC): 
C12N 9/14(2006.01)
 C12N 9/12(2006.01)
(86) International application number:
PCT/GB2013/051925
(87) International publication number:
WO 2014/013260 (23.01.2014 Gazette 2014/04)

(54)

MODIFIED HELICASES

MODIFIZIERTE HELIKASEN

HÉLICASES MODIFIÉES

(84) Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

(30) Priority: 19.07.2012 US 201261673452 P
08.03.2013 US 201361774862 P

(43) Date of publication of application:
27.05.2015 Bulletin 2015/22

(60) Divisional application:
20171363.3

(73) Proprietor: Oxford Nanopore Technologies Limited
Oxford OX4 4DQ (GB)

(72) Inventors:
  • HERON, Andrew
    Oxford Oxfordshire OX4 4GA (GB)
  • CLARKE, James
    Oxford Oxfordshire OX4 4GA (GB)
  • MOYSEY, Ruth
    Oxford Oxfordshire OX4 4GA (GB)
  • WALLACE, Elizabeth Jayne
    Oxford Oxfordshire OX4 4GA (GB)
  • BRUCE, Mark
    Oxford Oxfordshire OX4 4GA (GB)
  • JAYASINGHE, Lakmal
    Oxford Oxfordshire OX4 4GA (GB)
  • CAPROTTI, Domenico
    Oxford Oxfordshire OX4 4GA (GB)
  • SOEROES, Szabolcs
    Oxford Oxfordshire OX4 4GA (GB)
  • MCNEILL, Luke
    Oxford Oxfordshire OX4 4GA (GB)
  • ALVES, David
    Oxford Oxfordshire OX4 4GA (GB)
  • BOWEN, Rebecca
    Oxford Oxfordshire OX4 4GA (GB)
  • MILTON, John
    Oxford Oxfordshire OX4 4GA (GB)

(74) Representative: J A Kemp LLP 
14 South Square Gray's Inn
London WC1R 5JJ
London WC1R 5JJ (GB)


(56) References cited: : 
WO-A1-2010/004265
WO-A1-2010/086603
 WO-A1-2010/086602
WO-A2-2013/057495
   
  • DATABASE UniProt [Online] 30 November 2010 (2010-11-30), "RecName: Full=Putative ski2-type helicase; EC=3.6.4.-;", XP002712958, retrieved from EBI accession no. UNIPROT:E1QUS6 Database accession no. E1QUS6
  • DATABASE UniProt [Online] 11 July 2012 (2012-07-11), "SubName: Full=DEAD/DEAH box helicase;", XP002712959, retrieved from EBI accession no. UNIPROT:I3D0E7 Database accession no. I3D0E7
  • DATABASE UniProt [Online] 28 November 2012 (2012-11-28), "RecName: Full=Putative ski2-type helicase; EC=3.6.4.-;", XP002712960, retrieved from EBI accession no. UNIPROT:K0IM99 Database accession no. K0IM99
  • DATABASE UniProt [Online] 23 September 2008 (2008-09-23), "SubName: Full=GI23225;", XP002712961, retrieved from EBI accession no. UNIPROT:B4KAC8 Database accession no. B4KAC8
  • DATABASE REFSEQ [Online] NCBI; 7 June 2012 (2012-06-07), "activating signal cointegrator 1 complex subunit 3-like [Strongylocentrotus purpuratus];", XP002712962, Database accession no. XP_003728286
  • DATABASE UniProt [Online] 15 May 2007 (2007-05-15), "SubName: Full=Predicted protein;", XP002712963, retrieved from EBI accession no. UNIPROT:A4S1E1 Database accession no. A4S1E1
  • J. D. RICHARDS ET AL: "Structure of the DNA Repair Helicase Hel308 Reveals DNA Binding and Autoinhibitory Domains", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 283, no. 8, 1 February 2008 (2008-02-01), pages 5118-5126, XP55056802, ISSN: 0021-9258, DOI: 10.1074/jbc.M707548200
  • FAIRMAN-WILLIAMS M E ET AL: "SF1 and SF2 helicases: family matters", CURRENT OPINION IN STRUCTURAL BIOLOGY, ELSEVIER LTD, GB, vol. 20, no. 3, 1 June 2010 (2010-06-01), pages 313-324, XP027067341, ISSN: 0959-440X, DOI: 10.1016/J.SBI.2010.03.011 [retrieved on 2010-04-22] cited in the application
  • SCHNEIDER GRÉGORY F ET AL: "DNA sequencing with nanopores.", NATURE BIOTECHNOLOGY APR 2012, vol. 30, no. 4, April 2012 (2012-04), pages 326-328, XP002712964, ISSN: 1546-1696
  • Anonymous: "Press release: Oxford Nanopore introduces DNA 'strand sequencing' on the high-throughput GridION platform and presents MinION, a sequencer the size of a USB memory stick", , 17 February 2012 (2012-02-17), XP002712965, Retrieved from the Internet: URL:https://www.nanoporetech.com/news/pres s-releases/view/39 [retrieved on 2013-09-16]
  • GREEN N S ET AL: "Quantitative evaluation of the lengths of homobifunctional protein cross-linking reagents used as molecular rulers", PROTEIN SCIENCE, CAMBRIDGE UNIVERSITY PRESS, vol. 10, no. 1, 1 January 2001 (2001-01-01), pages 1293-1304, XP002415341, ISSN: 0961-8368, DOI: 10.1110/PS.51201
  • J. A. Ali ET AL: "Kinetic Measurement of the Step Size of DNA Unwinding by Escherichia coli UvrD Helicase", Science, vol. 275, no. 5298, 17 January 1997 (1997-01-17), pages 377-380, XP55492703, US ISSN: 0036-8075, DOI: 10.1126/science.275.5298.377
  • B. Theissen ET AL: "Cooperative binding of ATP and RNA induces a closed conformation in a DEAD box RNA helicase", Proceedings of the National Academy of Sciences, vol. 105, no. 2, 15 January 2008 (2008-01-15), pages 548-553, XP055493662, US ISSN: 0027-8424, DOI: 10.1073/pnas.0705488105
  • S. ARSLAN ET AL: "Engineering of a superhelicase through conformational control", SCIENCE, vol. 348, no. 6232, 17 April 2015 (2015-04-17), pages 344-347, XP55440958, US ISSN: 0036-8075, DOI: 10.1126/science.aaa0445
  • Martin R. Singleton ET AL: "Structure and Mechanism of Helicases and Nucleic Acid Translocases", Annual Review of Biochemistry, vol. 76, no. 1, 7 June 2007 (2007-06-07), pages 23-50, XP055056968, ISSN: 0066-4154, DOI: 10.1146/annurev.biochem.76.052305.115300
   
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