(19)
(11) EP 2 885 416 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
14.02.2024 Bulletin 2024/07

(45) Mention of the grant of the patent:
03.01.2024 Bulletin 2024/01

(21) Application number: 13830306.0

(22) Date of filing: 20.08.2013
(51) International Patent Classification (IPC): 
C12P 19/04(2006.01)
C12N 9/10(2006.01)
C12P 19/18(2006.01)
C12N 9/12(2006.01)
(52) Cooperative Patent Classification (CPC):
C12P 19/04; C12Y 207/0701; C12P 19/18; Y02P 20/55
(86) International application number:
PCT/US2013/055731
(87) International publication number:
WO 2014/031602 (27.02.2014 Gazette 2014/09)

(54)

LARGE SCALE ENZYMATIC SYNTHESIS OF OLIGOSACCHARIDES

ENZYMATISCHE SYNTHESE VON OLIGOSACCHARIDEN IM GROSSEN MASSSTAB

SYNTHÈSE ENZYMATIQUE À GRANDE ÉCHELLE D'OLIGOSACCHARIDES


(84) Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

(30) Priority: 20.08.2012 US 201261684974 P

(43) Date of publication of application:
24.06.2015 Bulletin 2015/26

(73) Proprietor: Academia Sinica
Taipei 11529 (TW)

(72) Inventors:
  • WONG, Chi-huey
    Rancho Santa Fe, CA 92067 (US)
  • WU, Chung-yi
    Taipei 115 (TW)
  • TSAI, Tsung-I
    Taipei 115 (TW)

(74) Representative: Cabinet Becker et Associés 
25, rue Louis le Grand
75002 Paris
75002 Paris (FR)


(56) References cited: : 
US-A1- 2002 034 805
US-A1- 2007 202 578
US-A1- 2007 202 578
US-A1- 2011 046 003
   
  • DORIS M. SU ET AL: "Enzymatic Synthesis of Tumor-Associated Carbohydrate Antigen Globo-H Hexasaccharide", ORGANIC LETTERS, vol. 10, no. 5, 1 March 2008 (2008-03-01), pages 1009-1012, XP055262837, US ISSN: 1523-7060, DOI: 10.1021/ol703121h
  • MUSLEH M. MUTHANA ET AL: "Efficient one-pot multienzyme synthesis of UDP-sugars using a promiscuous UDP-sugar pyrophosphorylase from Bifidobacterium longum (BLUSP)", CHEMICAL COMMUNICATIONS - CHEMCOM, vol. 48, no. 21, 1 January 2012 (2012-01-01), page 2728, XP055262842, GB ISSN: 1359-7345, DOI: 10.1039/c2cc17577k
  • JIANWEN FANG ET AL: "Highly Efficient Chemoenzymatic Synthesis of [alpha]-Galactosyl Epitopes with a Recombinant [alpha](1->3)-Galactosyltransferase", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 120, no. 27, 1 July 1998 (1998-07-01) , pages 6635-6638, XP055263251, US ISSN: 0002-7863, DOI: 10.1021/ja9808898
  • XI CHEN ET AL: "Sugar Nucleotide Regeneration Beads (Superbeads): A Versatile Tool for the Practical Synthesis of Oligosaccharides", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 123, no. 9, 1 March 2001 (2001-03-01) , pages 2081-2082, XP055263247, US ISSN: 0002-7863, DOI: 10.1021/ja005738v
  • J. SHAO ET AL: "Donor Substrate Regeneration for Efficient Synthesis of Globotetraose and Isoglobotetraose", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 68, no. 11, 1 November 2002 (2002-11-01), pages 5634-5640, XP055262721, US ISSN: 0099-2240, DOI: 10.1128/AEM.68.11.5634-5640.2002
  • TOSHIHISA KOTAKE ET AL: "Properties and physiological functions of UDP-sugar pyrophosphorylase in Arabidopsis", BIOSCIENCE BIOTECHNOLOGY BIOCHEMISTRY., vol. 71, no. 3, 1 March 2007 (2007-03-01), pages 761-771, XP055262839, TOKYO, JAPAN ISSN: 0916-8451, DOI: 10.1271/bbb.60605]
  • KOIZUMI, SATOSHI ET AL.: 'Large-scale production of UDP-galactose and globotriose by coupling metabolically engineered bacteria' NATURE BIOTECHNOLOGY vol. 16, no. 9, 1998, pages 847 - 850, XP055182862
  • LEE, JAE-HUN ET AL.: 'Otimization of the enzymatic one pot reaction for the synthesis of uridine 5' -diphosphogalactose' BIOPROCESS AND BIOSYSTEMS ENGINEERING vol. 33, no. 1, January 2010, pages 71 - 78, XP019781920
   
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