ATBECHDEDKESFRGBGRITLILUNLSEMCPTIESILTLVFIROMKCYALTRBGCZEEHUPLSK..HRIS..MTNORS..SM..................JBDM Ver 0.1.67 (18 Oct 2017) - 2100000/03048110EUROPEAN PATENT SPECIFICATIONB120191211EP14846349.02014091220160318koenen2013011150220130917KR20191211201950201607272016302019121120195020191023A61K 38/22 20060101AFI20190408BHEP A61P 17/00 20060101ALI20190408BHEP C07K 5/08 20060101ALI20190408BHEP C07K 5/10 20060101ALI20190408BHEP C07K 7/06 20060101ALI20190408BHEP deZUSAMMENSETZUNG MIT PEPTID AUS ADIPONECTINenCOMPOSITION COMPRISING PEPTIDE DERIVED FROM ADIPONECTINfrCOMPOSITION COMPRENANT UN PEPTIDE DÉRIVÉ D'ADIPONECTINEWO-A1-2005/042007JP-A- 2004 331 590JP-A- 2004 331 590JP-A- 2004 345 968JP-A- 2009 523 173KR-A- 20100 100 708US-A1- 2006 258 562LASZLO OTVOS JR ET AL: "Design and development of a peptide-based adiponectin receptor agonist for cancer treatment", BMC BIOTECHNOLOGY, BIOMED CENTRAL LTD. LONDON, GB, vol. 11, no. 1, 5 October 2011 (2011-10-05), page 90, XP021110409, ISSN: 1472-6750, DOI: 10.1186/1472-6750-11-90Anonymous: "SupadElixir & Features of SE-Tri-Peptides", , 15 December 2009 (2009-12-15), XP055344106, Retrieved from the Internet: URL:http://asia.in-cosmetics.com/__novadoc uments/305114?v=636135663896370000&v=63613 5663896370000 [retrieved on 2017-02-09]20170227HAHN, Jang-Hee105-903 80-7 Oesol-gil 19beon-gil Dongnae-myeonChuncheon-si Gangwon-do 200-883KRLIM, Dong-Young201 19 Aemakgol-gil 11beon-gilChuncheon-si Gangwon-do 200-936KRKNU-Industry Cooperation Foundation101380391CRT/61237 EP1 Gangwondaehak-gilChuncheon-si, Gangwon-do 200-701KRSupadelixir Inc.101516870CRT/61237 EPMedical School Building 1-410 Gangwondaehak-gilChuncheon-si, Kangwon-do 200-701KRTurner, Craig Robert100050564A.A. Thornton & Co. 10 Old BaileyLondon EC4M 7NGGBALATBEBGCHCYCZDEDKEEESFIFRGBGRHRHUIEISITLILTLULVMCMKMTNLNOPLPTRORSSESISKSMTRKR201400849720140912koWO201504143020150326201512 TECHNICAL FIELD

The present invention relates to a pharmaceutical or cosmetic composition comprising adiponectin-derived peptide fragments or small peptides. The peptides facilitate skin regeneration and moisturization, inhibit skin wrinkle, and have inhibitory activities against allergy and inflammation as well as metastasis of cancer cells.

BACKGROUND ART

Wounds, also referred to as skin lesions, show various symptoms, e.g., pain, bleeding, scar formation, dysfunction and so on. Wound healing involves processes such as inflammatory response, granulation tissue formation, re-epithelialization and angiogenesis etc. The processes include the actions of various growth factors, cytokines, neuronal hormones, etc. secreted from fibroblasts, adipocytes, blood-derived cells, and sensory neurons, leading to epidermal cell proliferation and extracellular matrix formation.

For regenerating connective tissues or maintaining the elasticity and strength of intact connective tissues without destruction thereof, it is important to form and maintain the extracellular matrix (ECM) proteins, such as type I, III collagens, elastin, fibronectin, etc., present in the skin. Natural aging or chronic exposure to ultraviolet radiation results in loss of the ECM proteins and reduced elasticity and strength of skin tissues, which are associated strongly with the characteristics of skin aging, such as wrinkle formation. Matrix metalloproteinases (MMPs) act as a key enzyme in degrading the dermal ECM. Over-expression of MMPs in keratinocytes and dermal fibroblasts under stress conditions, such as ultraviolet irradiation, contributes to degradation of the ECM proteins, thereby forming wrinkles on the skin.

An inflammatory response is known as a protective response of living organism for rehabilitating the structures and functions of tissues damaged by infection, trauma, etc. Mobilization of leukocytes to a focus of inflammation is critical for the rapid resolution of infections and restoration of tissue damages resulting from a variety of injuries. However, a misdirected or prolonged inflammatory response causes damage to the body's tissues or diseases. For example, inflammatory diseases are caused by bacterial or viral infection, e.g., cerebrospinal meningitis, enteritis, dermatitis, uveitis, encephalitis, or adult respiratory distress syndrome, or non-infectious factors, e.g., trauma, autoimmune diseases, or organ transplantation rejection. Inflammatory diseases are classified into acute and chronic inflammatory diseases according to symptoms or pathological features. Acute inflammation such as allergy or bacterial/viral infection is manifested as local signs such as a change in bloodstream, blood vessel size, and vascular permeability, and the recruitment of leukocytes. In contrast, a main pathological feature of chronic inflammation such as rheumatoid arthritis, artherosclerosis, chronic kidney infection, or hepatocirrhosis is a continuous emigration of macrophages, lymphocytes, or plasma cells into foci of inflammation due to recurrence of inflammatory factors, thereby causing a long-lasting inflammatory response.

In order to induce an inflammatory response, the emigration of leukocytes into inflammation foci is an essential event. Many cell adhesion molecules are implicated in the emigration of leukocytes. That is, the emigration of leukocytes includes a rolling stage in which leukocytes are mobilized to the blood vessels of inflamed sites by chemokine secreted from the inflamed sites and then rolled on surfaces of vascular endothelial cells while reducing the velocity of cell movement; an adhesion stage in which the leukocytes stops rolling and are firmly adhered to the vascular endothelial cells; and a transmigration stage wherein the leukocytes migrate through capillary vessels and basement membranes. The final stage, i.e., the transmigration stage is also called "diapedesis".

Cancer cells induced by carcinogens proliferate rapidly relative to normal cells, thereby forming tumor masses, invading surrounding tissues, and interfering with normal body functions. Cancer cells bring nutrients and oxygen by inducing angiogenesis, and metastasis thereof is also caused by angiogenesis. Although cancer cells grow infinitely at specific sites, they can also leave the sites from which they originated, migrate to and grow in new sites, whose process is called "metastasis". Metastasis involve several key steps: conversion of cancer cells to migratory mesenchymal cells, dissociation of the mesenchymal cells from the original tumor sites, invasion into and spread through surrounding connective tissues and capillary vessels, migration through blood vessels, escape from the blood vessels, migration through connective tissues, and proliferation in secondary sites.

Meanwhile, adiponectin is one of the adipokines, protein hormones secreted specifically in adipocytes. Adiponectin promotes the function of insulin and induces insulin resistance, thereby playing an important role in controlling cardiovascular diseases, such as hyperglycemia, hyperinsulinemia, obesity, and arteriosclerosis. And also, adiponectin has the functions for suppressing metastasis of cancer cells and inflammatory response. Adiponectin also performs the functions of wound healing, fibrosis inhibition, amelioration of skin wrinkle, and moisturization, by facilitating the expressions of filaggrin, hyaluronic acid, and extracellular matrix as well as the proliferation of keratinocytes in the skin.

DISCLOSURE Technical Problem

The present inventors have found that the specific peptide fragments or small peptides derived from adiponectin facilitate the proliferation of fibroblasts and induce the formation of extracellular matrix such as collagen, thereby exhibiting excellent activities for wound healing and skin regeneration. And also, the present inventors have found that the specific peptide fragments induce the formation of skin barrier by facilitating the expression of filaggrin, thereby exhibiting skin moisturizing activity. In addition, the present inventors have found that the specific peptide fragments inhibit allergic reactions and inflammatory responses (especially, inflammatory responses in the skin) through inhibiting the transmigration of leukocytes; and inhibit the metastasis of cancer cells through inhibiting the invasion of cancer cells.

Therefore, it is an object of the present invention to provide a pharmaceutical and cosmetic composition comprising the specific peptide fragments derived from adiponectin as an active ingredient.

Technical Solution

In accordance with an aspect of the present invention, there is provided a pharmaceutical composition for preventing or treating a skin disease, comprising a peptide selected from the group consisting of the peptides of SEQ ID NOs: 1 to 6 as an active ingredient and a pharmaceutically acceptable carrier.

In the pharmaceutical composition of the present invention, the skin disease may be dermatitis, skin wrinkle, wound, or dry skin; preferably one or more dermatitis selected from the group consisting of allergic dermatitis, atopic dermatitis, contact dermatitis, acne vulgaris, ultraviolet radiation-induced dermatitis, eczema, and psoriasis.

In accordance with another aspect of the present invention, there is provided a cosmetic composition for inhibiting or improving a skin disorder, comprising a peptide selected from the group consisting of the peptides of SEQ ID NOs: 1 to 6 as an active ingredient.

In the cosmetic composition of the present invention, the skin disorder may be dermatitis, skin wrinkle, or dry skin, preferably skin wrinkle or dry skin.

ADVANTAGEOUS EFFECTS

The peptides of the present invention, i.e., the peptides selected from the group consisting of the peptides of SEQ ID NOs: 1 to 6, facilitate the proliferation of fibroblasts and induce the formation of extracellular matrix such as collagen, thereby exhibiting excellent activities for wound healing and skin regeneration. And also, the peptides of the present invention induce the formation of skin barrier by facilitating the expression of filaggrin, thereby exhibiting skin moisturizing activity. Therefore, said peptides can be usefully applied to a pharmaceutical composition for wound healing or facilitating wound healing and a cosmetic composition for inhibiting wrinkle formation on the skin and for skin moisturization.

And also, the peptides of the present invention inhibit allergic reactions and inflammatory responses (especially, inflammatory responses in the skin) through inhibiting the transmigration of leukocytes; and inhibit the metastasis of cancer cells through inhibiting the invasion of cancer cells. Therefore, said peptides can be also usefully applied to a pharmaceutical composition for preventing or treating allergy or inflammatory diseases, a cosmetic composition for inhibiting or improving skin allergy or dermatitis, and a pharmaceutical composition for inhibiting metastasis of cancer cells.

BRIEF DESCRIPTION OF THE DRAWINGS

BEST MODE

Throughout the specification, the term "skin disease" includes all the skin diseases, especially the diseases caused by abnormalities in the adiponectin-mediated skin functions. Examples of the skin disease include dermatitis, skin wrinkle, wound, and dry skin. Said dermatitis includes allergic dermatitis, atopic dermatitis, contact dermatitis, acne vulgaris (or acneiform eruption), ultraviolet radiation-induced dermatitis, eczema, psoriasis, and so on. Said skin wrinkle includes the wrinkles according to UV stimulation and/or aging. Said wound includes a burn or a skin damage caused by UV stimulation. Said dry skin includes xeroderma, Sjogren's syndrome, and so on.

The term "skin disorder" includes all the skin disorders, especially the disorders caused by abnormalities in the adiponectin-mediated skin functions. Examples of the skin disorder include dermatitis, skin wrinkle, and dry skin. Said dermatitis includes allergic dermatitis, atopic dermatitis, contact dermatitis, acne vulgaris, ultraviolet radiation-induced dermatitis, eczema, psoriasis, and so on. Said skin wrinkle includes the wrinkles according to UV stimulation and/or aging. Said dry skin includes xeroderma, Sjogren's syndrome, and so on.

The present inventors prepared various fragments derived from adiponectin and investigated the activities thereof. Surprisingly, the present inventors have found that the specific small peptide fragments having 3 to 5 amino acids derived from adiponectin facilitate the proliferation of fibroblasts and induce the formation of extracellular matrix such as collagen, thereby exhibiting excellent activities for wound healing and skin regeneration. And also, the present inventors have found that said peptide fragments induce the formation of skin barrier by facilitating the expression of filaggrin, thereby exhibiting skin moisturizing activity. In addition, the present inventors have found that said peptide fragments inhibit allergic reactions and inflammatory responses (especially, inflammatory responses in the skin) through inhibiting the transmigration of leukocytes; and inhibit the metastasis of cancer cells (especially, skin cancer cells) through inhibiting the invasion of cancer cells. The peptide fragments are shown in the following Table 1. Table 1 Peptide name SEQ ID NO Amino acid sequence adipo-pep A4 SEQ ID NO: 1 Tyr-His-Ile-Thr adipo-pep A3 SEQ ID NO: 2 His-Ile-Thr adipo-pep B5 SEQ ID NO: 3 Leu-Phe-Thr-Tyr-Asp adipo-pep B4 SEQ ID NO: 4 Leu-Phe-Thr-Tyr adipo-pep B3 SEQ ID NO: 5 Phe-Thr-Tyr adipo-pep C3 SEQ ID NO: 6 His-Leu-Thr

Therefore, the present invention provides a pharmaceutical composition for preventing or treating a skin disease, comprising a peptide selected from the group consisting of the peptides of SEQ ID NOs: 1 to 6 as an active ingredient and a pharmaceutically acceptable carrier. In the pharmaceutical composition of the present invention, the skin disease may be dermatitis, skin wrinkle, wound, or dry skin; preferably one or more dermatitis selected from the group consisting of allergic dermatitis, atopic dermatitis, contact dermatitis, acne vulgaris, ultraviolet radiation-induced dermatitis, eczema, and psoriasis.

The present invention also provides a pharmaceutical composition for inhibiting cancer metastasis, preferably for inhibiting skin cancer metastasis, comprising a peptide selected from the group consisting of the peptides of SEQ ID NOs: 1 to 6 as an active ingredient and a pharmaceutically acceptable carrier.

The pharmaceutical composition of the present invention may include a pharmaceutically acceptable carrier, for example additives such as lactose or corn starch, lubricants such as magnesium stearate, currently available emulsifiers, suspending agents, buffers, isotonic agents, etc. The pharmaceutical composition of the present invention can be administered in an oral or a parenteral dosage form, preferably in a parenteral dosage form. For intramuscular, intraperitoneal, subcutaneous, or intravenous administration, a sterilized solution of an active ingredient is generally prepared. In this case, the sterilized solution may include a buffer to achieve a desired pH value. With respect to formulations for intravenous administration, an isotonic agent may be used to render the formulations isotonic. The pharmaceutical compositions of the present invention can be formulated into aqueous solutions including a pharmaceutically acceptable carrier such as a saline of pH 7.4. The aqueous solutions can be introduced into a patient's intramuscular blood stream by local bolus injection. The pharmaceutical composition of the present invention can be administered to patients who suffer from various skin diseases and cancers such as skin allergy, dermatitis, skin wrinkle, wound, dry skin, etc. at a daily dosage of about 1 to 2000 mg/kg. An adequate dosage is generally changed according to age, body weight, and conditions of a patient.

And also, the present invention includes within its scope a functional cosmetic composition comprising said peptides as an active ingredient. That is, the present invention provides a cosmetic composition for inhibiting or improving a skin disorder, comprising a peptide selected from the group consisting of the peptides of SEQ ID NOs: 1 to 6 as an active ingredient. In the cosmetic composition of the present invention, the skin disorder may be dermatitis, skin wrinkle, or dry skin, preferably skin wrinkle or dry skin.

The cosmetic composition of the present invention may be prepared in various forms according to conventional methods thereof. For example, the cosmetic composition may be prepared in forms of cosmetic products, cosmetic solutions, creams, lotions, etc., which may be diluted with a cleansing water, an astringent solution, or a moisture solution, for the use thereof. And also, the cosmetic composition may include conventional excipients, such as a stabilizer, a solubilizing agent, vitamin, a pigment, a flavoring agent, which are conventionally used in the field of cosmetic composition. In the cosmetic composition, said peptides may be present in an amount enough to provide the effects for improving skin allergy or inflammation, for example in an amount ranging from 0.001 to 10 weight %, preferably about 0.01 to 1 weight %, based on the total weight of the composition.

Hereinafter, the present invention will be described more specifically by the following examples and experimental examples. However, the following examples and experimental examples are provided only for illustrations and thus the present invention is not limited to or by them.

Example 1: Synthesis of peptides

The peptides of SEQ ID NOs: 1 to 6 (in the above Table 1) were synthesized with an automatic peptide synthesizer (PeptrEx-R48, Peptron, Daejeon, Korea) using a FMOC solid-phase method. The synthesized peptides were purified and analyzed by reverse-phase high-performance liquid chromatography (reverse-phase HPLC) (Prominence LC-20AB, Shimadzu, Japan) using a C18 analytical RP column (Shiseido capcell pak), and isolated using a mass spectrometer (HP 1100 Series LC/MSD, Hewlett-Packard, Roseville, U.S.A.).

Example 2: Preparation of peptide-containing compositions

The peptides of SEQ ID NOs: 1 to 6 were respectively dissolved in phosphate buffered saline (PBS) to a concentration of 1 M. The resultant protein solutions were diluted with PBS and then used in the following experimental examples.

Experimental Example 1: Tests for facilitating the proliferation of mouse fibroblast cell line NIH3T3

The NIH3T3 cells (mouse fibroblast cell line, ATCC CRL-1658, USA), along with 100 µℓ of Dulbecco's Modified Eagle medium (DMEM), were added to each well of the 96-well microplate in the concentration of 5 X 103 cells per well, and then cultured in a 5% CO2 incubator at 37°C for 24 hours. The peptide solutions (100 µℓ) having the respective peptide of SEQ ID NOs: 1, 2, 3 and 5 in the concentration of 10 µM were added to each well. While incubating the cells for 24, 48, and 72 hours therefrom, 10 µℓ of CCK-8 (Dojindo Laboratories, Japan) was added to each well and then the respective absorbance at 475 nm was measured with Spectramax plus 190 plate reader (Molecular Devices, USA). The results thereof are shown in FIG. 1. As shown in FIG. 1, the proliferations of NIH3T3 cells were significantly increased in the groups treated with the peptides of SEQ ID NOs: 1, 2, 3 and 5. These results show that the peptides of the present invention have wound-healing and skin-regenerating activities through facilitating the proliferation of fibroblasts.

Experimental Example 2: Evaluation on activation of the procollagen type-1 expression in fibroblasts

Since facilitation of the collagen fiber synthesis is one of the principal means for inhibiting skin aging, we evaluate whether the collagen synthesis in fibroblasts is facilitated by the treatment of the peptides of the present invention, using the NIH3T3 cells (mouse fibroblast cell line, ATCC CRL-1658, USA). The fibroblasts were treated with the peptides of SEQ ID NOs: 1, 2, 3 and 5, in the concentrations of 0.1, 1 and 10 µM, respectively. After 48 hours, the cell extracts were subject to the Western blotting assay so as to measure the expressions of procollagen type-1. The results thereof are shown in FIG. 2. As shown in FIG. 2, the expressions of procollagen type-1 were increased by the treatment of the peptides of SEQ ID NOs: 1, 2, 3 and 5 in concentration-dependent manner. Therefore, it can be seen that the peptides of the present invention can inhibit skin aging derived from loss of collagen, especially skin wrinkle.

Experimental Example 3: Evaluation on control of the profilaggrin expression in human keratinocyte cell line HaCaT

Since profilaggrin is one of the principal elements for constituting skin barrier, we evaluate whether the profilaggrin synthesis in keratinocytes is facilitated by the treatment of the peptides of the present invention, using the HaCaT cells (human keratinocyte cell line, DKFZ, Heidelberg, Germany). The HaCaT cells were treated with the peptides of SEQ ID NOs: 3 and 5, in the concentrations of 0.1, 1.0 and 10 µM. After 48 hours, the cell extracts were subject to the Western blotting assay so as to measure the expressions of profilaggrin. The results thereof are shown in FIG. 3. As shown in FIG. 3, the expressions of profilaggrin were increased by the treatment of the peptides of SEQ ID NOs: 3 and 5 in concentration-dependent manner. Therefore, it can be seen that the peptides of the present invention have a skin-moisturizing activity through facilitating the profilaggrin expression.

Experimental Example 4: Tests for inhibition of NF-κB activity

Effects of the peptides of the present invention on activation of the transcription factor NF-κB was analyzed according to the in situ proximity ligation assay method. The analysis was performed using the Duolink® In Situ reagent (Olink Bioscience, Sweden).

The NIH3T3 cells (2.5 × 104 cells) were plated in the 24-well plate, each well having a 12-mm glass in the bottom. The cells were stabilized for 24 hours and then treated with the peptides of SEQ ID NOs: 1, 2, 3 and 5, in the concentrations of 0.1, 1.0 and 10 µM. After 1 hour from the treatments, the cells of each well were treated with TNF-α (25 µg/ml) for 2 hours. After the cells fixed with 4% paraformaldehyde were treated with 0.1% Triton-X, a drop of the blocking solution was added to the cells, which were then incubated at 37 °C for 30 minutes. For measuring levels of the translocation of NF-κB into the nucleus, the cells were treated with the anti-p50 mouse monoclonal antibody (NFκB p50 (4D1): sc-53744, Santa Cruz Biotechnology, INC., USA) and the anti-p65 rabbit monoclonal antibody (NF-κB p65 (D14E12) XP® rabbit mAb, Cell signaling Technology, USA) at 37°C. After 30 minutes therefrom, the cells were treated with the PLA probe solution at 37°C for 1 hour, with the ligation solution for 30 minutes, and then with the amplification solution for 100 minutes. After washing the cells, the number of the spots translocated into the nucleus among the NF-κB shown in red spots was measured with a confocal microscopy. The results thereof are shown in FIGs. 4a to 4d. As shown in FIGs. 4a to 4d, the translocations in the groups treated with the peptides of SEQ ID NOs: 1 2, 3 and 5 were significantly reduced (about 25∼60% reduction) as compared with that in the control group. These results show that the peptides of the present invention inhibit activation of the NF-κB which plays a critical role in the synthesis of inflammatory cytokines.

Experimental Example 5: Tests for inhibitory activity against in vitro trans-endothelial migration of monocytes

Human umbilical vein endothelial cells (HUVECs) were cultured in the upper compartments of Boyden chambers. The supernatants were removed, and human monocytes (U937), which had been untreated or treated with the protein solutions including the peptide of SEQ ID NOs: 3 to 5 (30 µg/mℓ) prepared in Example 2 for 1 hour, were seeded at 2 x 105 cells/chamber. At this time, a culture including a supernatant obtained by centrifugation of the culture obtained after culturing NIH/3T3 mouse fibroblasts in serum-free DMEM containing 0.005% vitamin C and 0.1% Bovine Serum Albumin (BSA) for 16 hours was placed in the lower compartments of the chambers to induce the invasion of the monocytes. The chambers were incubated for 6 hours, and the number of the cells migrated to the lower compartments was measured. The test was repeated three times, and the results are shown in FIG. 5. The control group was treated with only PBS having no peptide. As shown in FIG. 5, the numbers of migrated monocytes in the groups treated with the peptides of the present invention were significantly reduced (about 50∼70% reduction) as compared with that in the control group. Taking into consideration that transmigration is essential for migration of leukocytes into inflammation sites through blood vessels, it is expected that peptides of the present invention can effectively inhibit the inflammatory reaction.

Experimental Example 6: Tests for inhibitory activity against invasion of cancer cells

After matrigel, basement membrane components, is subject to polymerization reaction in a transwell, human breast cancer cells MCF-7 cells (1.5 x 105 cells) were loaded to the upper compartment of the transwell and then treated with each peptide of SEQ ID NOs: 3 to 5 in the concentration of 1 µM. The cells were cultured in a 5% CO2 incubator at 37 °C. 0.1% BSA was added to the upper compartment of the transwell. After an invasion-inducing medium (the supernatant isolated after culturing NIH 3T3 cells in the serum-free DMEM containing 0.005% vitamin C and 0.1% bovine serum albumin for 24 hours) was placed in each lower compartment, cells migrated into the lower compartments of the transwell were counted three times at 24-hour intervals, and then the results were statistically analyzed. The control group was treated with only PBS having no peptide. The results thereof are shown in FIG. 6. As shown in FIG. 6, the base membrane invasion rates of the human breast cancer cells in the groups treated with the peptides of SEQ ID NOs: 3 to 5 of the present invention were reduced by about 80% as compared with that in the control group. Taking into consideration that cancer cells come out from blood vessels and invade basement membranes or surrounding connective tissues and then spread to secondary sites, it can be seen that the peptides of the present invention can effectively inhibit the metastasis of cancer cells.

A pharmaceutical composition suitable for preventing or treating a skin disease, comprising a peptide selected from the group consisting of the peptides of SEQ ID NOs: 1 to 6 as an active ingredient and a pharmaceutically acceptable carrier. A pharmaceutical composition according to claim 1, wherein the skin disease is dermatitis, skin wrinkle, wound, or dry skin. A pharmaceutical composition according to claim 2, wherein the skin disease is one or more dermatitis selected from the group consisting of allergic dermatitis, atopic dermatitis, contact dermatitis, acne vulgaris, ultraviolet radiation-induced dermatitis, eczema, and psoriasis. A pharmaceutical composition according to any one of claims 1 to 3, wherein the carrier is selected from: additives, optionally lactose and corn starch, lubricants, optionally magnesium stearate, emulsifiers, suspending agents, buffers, isotonic agents and aqueous solutions, optionally saline. A cosmetic composition suitable for inhibiting or improving a skin disorder, comprising a peptide selected from the group consisting of the peptides of SEQ ID NOs: 1 to 6 as an active ingredient. A cosmetic composition according to claim 5, wherein the skin disorder is dermatitis, skin wrinkle, or dry skin. A cosmetic composition according to claim 6, wherein the skin disorder is skin wrinkle or dry skin. A cosmetic composition according to any one of claims 5 to 7, further comprising an excipient, optionally a stabilizer, a solubilizing agent, vitamin, a pigment or a flavouring agent. A cosmetic or pharmaceutical composition according to any one of the preceding claims, wherein the peptide is the peptide of SEQ ID NO: 1. A cosmetic or pharmaceutical composition according to any one of claims 1 to 8, wherein the peptide is the peptide of SEQ ID NO: 2. A cosmetic or pharmaceutical composition according to any one of claims 1 to 8, wherein the peptide is the peptide of SEQ ID NO: 3. A cosmetic or pharmaceutical composition according to any one of claims 1 to 8, wherein the peptide is the peptide of SEQ ID NO: 4. A cosmetic or pharmaceutical composition according to any one of claims 1 to 8, wherein the peptide is the peptide of SEQ ID NO: 5. A cosmetic or pharmaceutical composition according to any one of claims 1 to 8, wherein the peptide is the peptide of SEQ ID NO: 6. Pharmazeutische Zusammensetzung, die zum Verhindern oder Behandeln einer Hauterkrankung geeignet ist, umfassend ein Peptid, ausgewählt aus der Gruppe bestehend aus den Peptiden der SEQ ID NRn: 1 bis 6 als ein Wirkstoff und ein pharmazeutisch unbedenklicher Träger. Pharmazeutische Zusammensetzung nach Anspruch 1, wobei die Hautkrankheit Dermatitis, faltige Haut, eine Wunde oder trockene Haut ist. Pharmazeutische Zusammensetzung nach Anspruch 2, wobei die Hautkrankheit eine Dermatitis oder mehrere Dermatitiden sind, ausgewählt aus der Gruppe bestehend aus allergischer Dermatitis, atopischer Dermatitis, Kontaktdermatitis, Akne vulgaris, durch ultraviolette Strahlung induzierter Dermatitis, Ekzem und Psoriasis. Pharmazeutische Zusammensetzung nach einem der Ansprüche 1 bis 3, wobei der Träger ausgewählt wird aus: Additiven, gegebenenfalls Laktose und Maisstärke, Gleitmitteln, gegebenenfalls Magnesiumstearat, Emulgatoren, Suspensionsmitteln, Puffern, isotonischen Mitteln und wässrigen Lösungen, gegebenenfalls Kochsalzlösung. Kosmetische Zusammensetzung, die zum Hemmen oder Verbessern einer Hauterkrankung geeignet ist, umfassend ein Peptid, ausgewählt aus der Gruppe bestehend aus Peptiden der SEQ ID NR: 1 bis 6 als ein Wirkstoff. Kosmetische Zusammensetzung nach Anspruch 5, wobei die Hauterkrankung Dermatitis, faltige Haut oder trockene Haut ist. Kosmetische Zusammensetzung nach Anspruch 6, wobei die Hauterkrankung faltige Haut oder trockene Haut ist. Kosmetische Zusammensetzung nach einem der Ansprüche 5 bis 7, ferner umfassend einen Hilfsstoff, gegebenenfalls einen Stabilisator, ein Lösungsvermittler, ein Vitamin, ein Pigment oder einen Aromastoff. Kosmetische oder pharmazeutische Zusammensetzung nach einem der vorhergehenden Ansprüche, wobei das Peptid das Peptid der SEQ ID NR: 1 ist. Kosmetische oder pharmazeutische Zusammensetzung nach einem der Ansprüche 1 bis 8, wobei das Peptid das Peptid der SEQ ID NR: 2 ist. Kosmetische oder pharmazeutische Zusammensetzung nach einem der Ansprüche 1 bis 8, wobei das Peptid das Peptid der SEQ ID NR: 3 ist. Kosmetische oder pharmazeutische Zusammensetzung nach einem der Ansprüche 1 bis 8, wobei das Peptid das Peptid der SEQ ID NR: 4 ist. Kosmetische oder pharmazeutische Zusammensetzung nach einem der Ansprüche 1 bis 8, wobei das Peptid das Peptid der SEQ ID NR: 5 ist. Kosmetische oder pharmazeutische Zusammensetzung nach einem der Ansprüche 1 bis 8, wobei das Peptid das Peptid der SEQ ID NR: 6 ist. Composition pharmaceutique appropriée pour prévenir ou traiter une maladie cutanée, comprenant un peptide choisi parmi le groupe constitué des peptides de SEQ ID No : 1 à 6 en tant que principe actif et un transporteur pharmaceutiquement acceptable. Composition pharmaceutique selon la revendication 1, dans laquelle la maladie cutanée est la dermatite, la peau ridée, la blessure ou la peau sèche. Composition pharmaceutique selon la revendication 2, dans laquelle la maladie cutanée est une ou plusieurs dermatites choisies dans le groupe comprenant la dermatite allergique, la dermatite atopique, la dermatite de contact, l'acné vulgaire, la dermatite induite par les rayons ultraviolets, l'eczéma et le psoriasis. Composition pharmaceutique selon l'une quelconque des revendications 1 à 3, dans laquelle le transporteur est choisi parmi : des additifs, éventuellement du lactose et de l'amidon de maïs, des lubrifiants, éventuellement du stéarate de magnésium, des émulsifiants, des agents de suspension, des tampons, des agents isotoniques et des solutions aqueuses, éventuellement du sérum physiologique. Composition cosmétique appropriée pour inhiber ou améliorer un trouble de la peau, comprenant un peptide choisi parmi le groupe constitué des peptides de SEQ ID No : 1 à 6 en tant que principe actif. Composition cosmétique selon la revendication 5, dans laquelle le trouble de la peau est la dermatite, la peau ridée ou la peau sèche. Composition cosmétique selon la revendication 6, dans laquelle le trouble de la peau est la peau ridée ou la peau sèche. Composition cosmétique selon l'une quelconque des revendications 5 à 7, comprenant en outre un excipient, éventuellement un stabilisateur, un agent solubilisant, de la vitamine, un pigment ou un agent aromatisant. Composition cosmétique ou pharmaceutique selon l'une quelconque des revendications précédentes, dans laquelle le peptide est le peptide de SEQ ID NO : 1. Composition cosmétique ou pharmaceutique selon l'une quelconque des revendications 1 à 8, dans laquelle le peptide est le peptide de SEQ ID NO : 2. Composition cosmétique ou pharmaceutique selon l'une quelconque des revendications 1 à 8, dans laquelle le peptide est le peptide de SEQ ID NO : 3. Composition cosmétique ou pharmaceutique selon l'une quelconque des revendications 1 à 8, dans laquelle le peptide est le peptide de SEQ ID NO : 4. Composition cosmétique ou pharmaceutique selon l'une quelconque des revendications 1 à 8, dans laquelle le peptide est le peptide de SEQ ID NO : 5. Composition cosmétique ou pharmaceutique selon l'une quelconque des revendications 1 à 8, dans laquelle le peptide est le peptide de SEQ ID NO : 6.
REFERENCES CITED IN THE DESCRIPTION

This list of references cited by the applicant is for the reader's convenience only. It does not form part of the European patent document. Even though great care has been taken in compiling the references, errors or omissions cannot be excluded and the EPO disclaims all liability in this regard.

Patent documents cited in the description