(19)
(11) EP 3 132 035 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
22.04.2020 Bulletin 2020/17

(45) Mention of the grant of the patent:
15.01.2020 Bulletin 2020/03

(21) Application number: 15718987.9

(22) Date of filing: 16.04.2015
(51) International Patent Classification (IPC): 
C12N 15/10(2006.01)
C12N 15/74(2006.01)
(86) International application number:
PCT/GB2015/051152
(87) International publication number:
WO 2015/159086 (22.10.2015 Gazette 2015/42)

(54)

DELETION MUTATIONS

DELETIONSMUTATIONEN

MUTATIONS PAR DÉLÉTION


(84) Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

(30) Priority: 17.04.2014 GB 201406968

(43) Date of publication of application:
22.02.2017 Bulletin 2017/08

(73) Proprietor: Biocleave Limited
Abingdon, Oxfordshire OX14 4SD (GB)

(72) Inventors:
  • KRABBEN, Preben
    Didcot Oxfordshire OX11 8FD (GB)
  • JENKINSON, Elizabeth
    Abingdon Oxfordshire OX14 4SD (GB)

(74) Representative: Dehns 
St. Bride's House 10 Salisbury Square
London EC4Y 8JD
London EC4Y 8JD (GB)


(56) References cited: : 
WO-A1-2013/141680
WO-A1-2014/143381
WO-A2-2013/133882
WO-A1-2013/142578
WO-A1-2014/191518
   
  • ANN RAN F ET AL: "Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity", CELL, CELL PRESS, US, vol. 154, no. 6, 12 September 2013 (2013-09-12), pages 1380-1389, XP002718603, ISSN: 0092-8674, DOI: 10.1016/J.CELL.2013.08.021 [retrieved on 2013-08-29]
  • L. CONG ET AL: "Multiplex Genome Engineering Using CRISPR/Cas Systems", SCIENCE, vol. 339, no. 6121, 15 February 2013 (2013-02-15), pages 819-823, XP55181426, ISSN: 0036-8075, DOI: 10.1126/science.1231143 -& L. CONG ET AL: "Multiplex Genome Engineering Using CRISPR/Cas Systems, Supplementary Materials", SCIENCE, vol. 339, no. 6121, 3 January 2013 (2013-01-03), pages 1-27, XP55153567, ISSN: 0036-8075, DOI: 10.1126/science.1231143
  • M. A. AL-HINAI ET AL: "Novel System for Efficient Isolation of Clostridium Double-Crossover Allelic Exchange Mutants Enabling Markerless Chromosomal Gene Deletions and DNA Integration", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 78, no. 22, 14 September 2012 (2012-09-14), pages 8112-8121, XP055177560, ISSN: 0099-2240, DOI: 10.1128/AEM.02214-12
  • JIANG WENYAN ET AL: "RNA-guided editing of bacterial genomes using CRISPR-Cas systems", NATURE BIOTECHNOLOGY,, vol. 31, no. 3, 1 March 2013 (2013-03-01), pages 233-239, XP002699849, & JIANG WENYAN ET AL: "RNA-guided editing of bacterial genomes using CRISPR-Cas systems, Supplementary Text and Figures;", NATURE BIOTECHNOLOGY MAR 2013, vol. 31, no. 3, March 2013 (2013-03), pages 1-21, ISSN: 1546-1696
  • HEAP JOHN T ET AL: "Development of Genetic Knock-out Systems for Clostridia", 1 January 2009 (2009-01-01), CLOSTRIDIA : MOLECULAR BIOLOGY IN THE POST-GENOMIC ERA,, PAGE(S) 179 - 198, XP009116784, ISBN: 978-1-904455-38-7 the whole document
  • ZHONG JIN ET AL: "Targeted and random bacterial gene disruption using a group II intron (targetron) vector containing a retrotransposition-activated selectable marker", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, GB, vol. 31, no. 6, 1 March 2003 (2003-03-01), pages 1656-1664, XP002456213, ISSN: 0305-1048, DOI: 10.1093/NAR/GKG248
  • JOSIANE E. GARNEAU ET AL: "The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA", NATURE, vol. 468, no. 7320, 4 November 2010 (2010-11-04), pages 67-71, XP55181397, ISSN: 0028-0836, DOI: 10.1038/nature09523
  • RICHTER HAGEN ET AL: "Characterization of CRISPR RNA processing in Clostridium thermocellum and Methanococcus maripaludis.", NUCLEIC ACIDS RESEARCH OCT 2012, vol. 40, no. 19, October 2012 (2012-10), pages 9887-9896, XP002741441, ISSN: 1362-4962
  • J. A. DOUDNA ET AL: "The new frontier of genome engineering with CRISPR-Cas9", SCIENCE, vol. 346, no. 6213, 28 November 2014 (2014-11-28), pages 1258096-1258096, XP055162699, ISSN: 0036-8075, DOI: 10.1126/science.1258096
   
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