(19)
(11) EP 3 132 036 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
22.04.2020 Bulletin 2020/17

(45) Mention of the grant of the patent:
15.01.2020 Bulletin 2020/03

(21) Application number: 15718988.7

(22) Date of filing: 16.04.2015
(51) International Patent Classification (IPC): 
C12N 15/10(2006.01)
C12N 15/90(2006.01)
 C12N 15/74(2006.01)
(86) International application number:
PCT/GB2015/051153
(87) International publication number:
WO 2015/159087 (22.10.2015 Gazette 2015/42)

(54)

TARGETED MUTATIONS

GEZIELTE MUTATIONEN

MUTATIONS CIBLEES

(84) Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

(30) Priority: 17.04.2014 GB 201406970

(43) Date of publication of application:
22.02.2017 Bulletin 2017/08

(73) Proprietor: Biocleave Limited
Abingdon, Oxfordshire OX14 4SD (GB)

(72) Inventors:
  • JENKINSON, Elizabeth
    Abingdon Oxfordshire OX14 4SD (GB)
  • KRABBEN, Preben
    Didcot Oxfordshire OX11 8FD (GB)

(74) Representative: Dehns 
St. Bride's House 10 Salisbury Square
London EC4Y 8JD
London EC4Y 8JD (GB)


(56) References cited: : 
WO-A1-2008/040387
WO-A1-2014/191518
WO-A2-2007/148091
 WO-A1-2014/143381
WO-A1-2015/040402
WO-A2-2009/101400
   
  • JIANG ET AL.: "RNA-guided editing of bacterial genomes using CRISPR-Cas systems", NATURE BIOTECHNOLOGY, vol. 31, 2013, pages 233-239, XP002699849, cited in the application
  • WANG YI ET AL: "Markerless chromosomal gene deletion in Clostridium beijerinckii using CRISPR/Cas9 system.", JOURNAL OF BIOTECHNOLOGY, vol. 200, 20 April 2015 (2015-04-20), pages 1-5, XP002741440, ISSN: 1873-4863
  • RAN F ANN ET AL: "Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity", CELL, vol. 154, no. 6, 12 September 2013 (2013-09-12), pages 1380-1389, XP028716272, ISSN: 0092-8674, DOI: 10.1016/J.CELL.2013.08.021
  • L. CONG ET AL: "Multiplex Genome Engineering Using CRISPR/Cas Systems", SCIENCE, vol. 339, no. 6121, 15 February 2013 (2013-02-15), pages 819-823, XP055181426, ISSN: 0036-8075, DOI: 10.1126/science.1231143 -& L. CONG ET AL: "Supplementary Materials for Multiplex Genome Engineering Using CRISPR/Cas Systems", SCIENCE, vol. 339, no. 6121, 3 January 2013 (2013-01-03), page 28PP, XP055153567, ISSN: 0036-8075, DOI: 10.1126/science.1231143
  • RICHTER HAGEN ET AL: "Characterization of CRISPR RNA processing in Clostridium thermocellum and Methanococcus maripaludis.", NUCLEIC ACIDS RESEARCH, vol. 40, no. 19, October 2012 (2012-10), pages 9887-9896, XP002741441, ISSN: 1362-4962
  • JOSIANE E. GARNEAU ET AL: "The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA", NATURE, vol. 468, no. 7320, 4 November 2010 (2010-11-04), pages 67-71, XP055181397, ISSN: 0028-0836, DOI: 10.1038/nature09523
  • HAGEN RICHTER ET AL: "Exploiting CRISPR/Cas: Interference Mechanisms and Applications", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 14, no. 7, 12 July 2013 (2013-07-12), pages 14518-14531, XP055153313, DOI: 10.3390/ijms140714518
  • XU TAO ET AL: "Efficient Genome Editing in Clostridium cellulolyticum via CRISPR-Cas9 Nickase.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 81, no. 13, 24 April 2015 (2015-04-24), pages 4423-4431, XP002741442, ISSN: 1098-5336 -& XU TAO ET AL: "Supplementary Data for Efficient Genome Editing in Clostridium cellulolyticum via CRISPR-Cas9 Nickase.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 24 April 2015 (2015-04-24), page 13PP, XP002741729,
  • RUTH KIRO ET AL: "Efficient engineering of a bacteriophage genome using the type I-E CRISPR-Cas system", RNA BIOLOGY, vol. 11, no. 1, 1 January 2014 (2014-01-01), pages 42-44, XP055385263, US ISSN: 1547-6286, DOI: 10.4161/rna.27766
  • Michiel Vos ET AL: "A comparison of homologous recombination rates in bacteria and archaea", The I S M E Journal: multidisciplinary journal of microbial ecology, vol. 3, no. 2, 2 October 2008 (2008-10-02) , pages 199-208, XP055493591, United Kingdom ISSN: 1751-7362, DOI: 10.1038/ismej.2008.93
   
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