(19)
(11) EP 3 140 421 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
06.05.2020 Bulletin 2020/19

(45) Mention of the grant of the patent:
25.03.2020 Bulletin 2020/13

(21) Application number: 15723189.5

(22) Date of filing: 08.05.2015
(51) International Patent Classification (IPC): 
C12Q 1/6827(2018.01)
C12Q 1/6883(2018.01)
C12Q 1/6886(2018.01)
(86) International application number:
PCT/EP2015/060188
(87) International publication number:
WO 2015/169947 (12.11.2015 Gazette 2015/45)

(54)

DETECTION OF DNA THAT ORIGINATES FROM A SPECIFIC CELL-TYPE AND RELATED METHODS

NACHWEIS VON AUS EINEM SPEZIFISCHEN ZELLTYP STAMMENDER DNA

DÉTECTION D'ADN QUI PROVIENT D'UN TYPE CELLULAIRE SPÉCIFIQUE


(84) Designated Contracting States:
BE CH DE FR GB IE LI LU MC
Designated Extension States:
BA ME
Designated Validation States:
MA

(30) Priority: 09.05.2014 EP 14167769
09.05.2014 EP 14167775

(43) Date of publication of application:
15.03.2017 Bulletin 2017/11

(60) Divisional application:
18214042.6 / 3521454

(73) Proprietor: Eurofins LifeCodexx GmbH
78467 Konstanz (DE)

(72) Inventors:
  • GRÖMMINGER, Sebastian
    78467 Konstanz (DE)
  • HOFMANN, Wera
    78467 Konstanz (DE)
  • SAID, Hamed
    78467 Konstanz (DE)
  • SACHSE, Matthias
    78467 Konstanz (DE)

(74) Representative: Dörries, Hans Ulrich 
df-mp Dörries Frank-Molnia & Pohlman Patentanwälte Rechtsanwälte PartG mbB Theatinerstrasse 16
80333 München
80333 München (DE)


(56) References cited: : 
WO-A1-2011/018600
WO-A2-2005/118852
WO-A2-2011/092592
WO-A1-2011/034631
WO-A2-2010/033639
   
  • CAMPAN MIHAELA ET AL: "MethyLight.", METHODS IN MOLECULAR BIOLOGY (CLIFTON, N.J.) 2009, vol. 507, 2009, pages 325-337, XP002730440, ISSN: 1064-3745
  • WEISENBERGER D J ET AL: "Analysis of repetitive element DNA methylation by MethyLight", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, GB, vol. 33, no. 21, 1 January 2005 (2005-01-01), pages 6823-6836, XP002422120, ISSN: 0305-1048, DOI: 10.1093/NAR/GKI987
  • HE Q ET AL: "Development of a multiplex MethyLight assay for the detection of multigene methylation in human colorectal cancer", CANCER GENETICS AND CYTOGENETICS, ELSEVIER SCIENCE PUBLISHING, NEW YORK, NY, US, vol. 202, no. 1, 1 October 2010 (2010-10-01), pages 1-10, XP027246507, ISSN: 0165-4608 [retrieved on 2010-08-27]
  • EKATERINA OLKHOV-MITSEL ET AL: "Novel Multiplex MethyLight Protocol for Detection of DNA Methylation in Patient Tissues and Bodily Fluids", SCIENTIFIC REPORTS, vol. 4, 21 March 2014 (2014-03-21), XP055143311, DOI: 10.1038/srep04432
  • SUZANNE SNELLENBERG ET AL: "Development of a multiplex methylation-specific PCR as candidate triage test for women with an HPV-positive cervical scrape", BMC CANCER, BIOMED CENTRAL, LONDON, GB, vol. 12, no. 1, 23 November 2012 (2012-11-23), page 551, XP021128495, ISSN: 1471-2407, DOI: 10.1186/1471-2407-12-551
  • WEISENBERGER DANIEL J ET AL: "DNA methylation analysis by digital bisulfite genomic sequencing and digital MethyLight", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, GB, vol. 36, no. 14, 1 August 2008 (2008-08-01), pages 4689-4698, XP002585338, ISSN: 1362-4962, DOI: 10.1093/NAR/GKN455 [retrieved on 2008-07-15]
  • CHAN K C ALLEN ET AL: "Hypermethylated RASSF1A in maternal plasma: A universal fetal DNA marker that improves the reliability of noninvasive prenatal diagnosis", CLINICAL CHEMISTRY, AMERICAN ASSOCIATION FOR CLINICAL CHEMISTRY, WASHINGTON, DC, vol. 52, no. 12, 1 December 2006 (2006-12-01), pages 2211-2218, XP009089108, ISSN: 0009-9147, DOI: 10.1373/CLINCHEM.2006.074997
  • A. O. H. NYGREN ET AL: "Quantification of Fetal DNA by Use of Methylation-Based DNA Discrimination", CLINICAL CHEMISTRY, vol. 56, no. 10, 20 August 2010 (2010-08-20), pages 1627-1635, XP055089811, ISSN: 0009-9147, DOI: 10.1373/clinchem.2010.146290
  • THERESA SWIFT-SCANLAN ET AL: "Two-color quantitative multiplex methylation-specific PCR", BIOTECHNIQUES, vol. 40, no. 2, 1 February 2006 (2006-02-01), pages 210-219, XP055143300, ISSN: 0736-6205, DOI: 10.2144/000112097
   
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