(19)
(11) EP 3 143 410 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
05.06.2019 Bulletin 2019/23

(45) Mention of the grant of the patent:
02.01.2019 Bulletin 2019/01

(21) Application number: 15727124.8

(22) Date of filing: 12.05.2015
(51) International Patent Classification (IPC): 
G01N 33/68(2006.01)
C12Q 1/68(2018.01)
G01N 33/80(2006.01)
C12N 5/00(2006.01)
C12N 5/078(2010.01)
(86) International application number:
PCT/US2015/030420
(87) International publication number:
WO 2015/175562 (19.11.2015 Gazette 2015/46)

(54)

PREPARATION OF FETAL NUCLEATED RED BLOOD CELLS (NRBCS) FOR DIAGNOSTIC TESTING

HERSTELLUNG FETALER NUKLEIERTER ERYTHROZYTEN FÜR DIAGNOSTISCHE TESTS

PRÉPARATION DE GLOBULES ROUGES NUCLÉÉES F TALES (NRBC) POUR TEST DE DIAGNOSTIC


(84) Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated Extension States:
BA ME

(30) Priority: 15.05.2014 US 201461993659 P

(43) Date of publication of application:
22.03.2017 Bulletin 2017/12

(60) Divisional application:
18248180.4 / 3489691

(73) Proprietor: KellBenx Inc.
Great River, NY 11739 (US)

(72) Inventors:
  • BENNANI, Hassan
    Dix Hills, New York 11746 (US)
  • KELLNER, Leonard H.
    Massapequa, New York 11758 (US)

(74) Representative: Hoffmann Eitle 
Patent- und Rechtsanwälte PartmbB Arabellastraße 30
81925 München
81925 München (DE)


(56) References cited: : 
WO-A1-2004/076653
US-A1- 2013 122 492
   
  • PURWOSUNU Y ET AL: "Clinical Potential for Noninvasive Prenatal Diagnosis Through Detection of Fetal Cells in Maternal Blood", TAIWANESE JOURNAL OF OBSTETRICS AND GYNECOLOGY, ELSEVIER (SINGAPORE) PTE LTD, HONG KONG BRANCH, HONG KONG, HK, vol. 45, no. 1, 1 March 2006 (2006-03-01), pages 10-20, XP026304744, ISSN: 1028-4559, DOI: 10.1016/S1028-4559(09)60184-4 [retrieved on 2006-03-01]
  • BIANCHI D W ET AL: "ISOLATION OF FETAL DNA FROM NUCLEATED ERYTHROCYTES IN MATERNAL BLOOD", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, US, vol. 87, no. 9, 1 May 1990 (1990-05-01), pages 3279-3283, XP000226739, ISSN: 0027-8424, DOI: 10.1073/PNAS.87.9.3279
  • MARY ANN DEMARIA ET AL: "Improved fetal nucleated erythrocyte sorting purity using intracellular antifetal hemoglobin and Hoechst 33342", CYTOMETRY, vol. 25, 1 September 1996 (1996-09-01), pages 37-45, XP055200753,
  • ZHENG Y L ET AL: "Flow sorting of fetal erythroblasts using intracytoplasmic anti-fetal haemoglobin: preliminary observations on maternal samples", PRENATAL DIAGNOSIS, CHICHESTER, SUSSEX, GB, vol. 15, no. 10, 1 October 1995 (1995-10-01), pages 897-905, XP009168364, ISSN: 0197-3851
  • BÜSCH J ET AL: "Enrichment of fetal cells from maternal blood by high gradient magnetic cell sorting (double MACS) for PCR-based genetic analysis", PRENATAL DIAGNOSIS, CHICHESTER, SUSSEX, GB, vol. 14, no. 12, 1 December 1994 (1994-12-01), pages 1129-1140, XP002513168, ISSN: 0197-3851, DOI: 10.1002/PD.1970141206
  • JI YI WANG ET AL: "Fetal nucleated erythrocyte recovery: Fluorescence activated cell sorting-based positive selection using anti-gamma globin versus magnetic activated cell sorting using anti-CD45 depletion and anti-gamma globin positive selection", CYTOMETRY, vol. 39, no. 3, 1 March 2000 (2000-03-01), pages 224-230, XP055201215, ISSN: 0196-4763, DOI: 10.1002/(SICI)1097-0320(20000301)39:3<224: :AID-CYTO8>3.0.CO;2-J
  • SEKIZAWA ET AL: "Fetal cell recycling: Diagnosis of gender and RhD genotype in the same fetal cell retrieved from maternal blood", AMERICAN JOURNAL OF OBSTETRICS & GYNECOLOGY, MOSBY, ST LOUIS, MO, US, vol. 181, no. 5, 1 November 1999 (1999-11-01), pages 1237-1242, XP005691139, ISSN: 0002-9378, DOI: 10.1016/S0002-9378(99)70115-8
   
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