                         SEQUENCE LISTING

<110>  Matrix Genetics
 
<120>  Targeted Mutagenesis in Spirulina

<130>  M077-0017USP1

<160>  27    

<170>  PatentIn version 3.5

<210>  1
<211>  6994
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pApl-pilA plasmid backbone including addA gene with homology arms
       and restriction sites for Asd (GGCGCGCC) and EcoRI (GAATTC)

<400>  1
gaattcacat acgcggccgc ctgggccttg agctcgaatt tcctgcatta atgaatcggc       60

caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac      120

tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata      180

cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa      240

aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct      300

gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa      360

agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg      420

cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca      480

cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa      540

ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg      600

gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg      660

tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga      720

acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc      780

tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag      840

attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac      900

gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc      960

ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag     1020

taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt     1080

ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag     1140

ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca     1200

gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact     1260

ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca     1320

gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg     1380

tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc     1440

atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg     1500

gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca     1560

tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt     1620

atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc     1680

agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc     1740

ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca     1800

tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa     1860

aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat     1920

tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa     1980

aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa     2040

accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc     2100

gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca     2160

gcttgtctgt aagcggatgc caagcttgca tgcctgcagg tggcgcgccg aatgacgcta     2220

acagcaggac ctatattggg aattaggttg aggagtccgg cgagaatggc attggcgaaa     2280

attaagggga ctctcagtat ccacaagccg actactgtta gtagggtgac accaatcatg     2340

ttgagtaagg caccgattag ccaacgacct agggctattt cgcaagtatc gagaatgtcg     2400

ttcattcgcc ttcggtaaaa ggcgggaatt agttgtaagc tgctgcgacg gtaggcttgg     2460

gggtcggcta aaagcatgag ggtgaggacg aggattaata agatgctgac taatccttct     2520

actgtggttc cgacgacgac cccaaaaccg ccgattaatt gattgctgag ggcttgggct     2580

tgttggataa tttgttcgat gcggatctgt ctcaggtcgc gactgacttc tgatagatat     2640

tcccgtaagg gtccgttgag gtcggtgcgt aaatagtcaa ttaacaggtt cagcagcttt     2700

agaccttgtt cgagaccggc ggggacctgt ttggctagtt gttctaattg gttgattagg     2760

ggggggacta ccaataaaaa gccgatggta atccctaaga agaatagact aatggatagg     2820

aagacggcaa gcgatcgcgg tagtttcaga cgctggaatt gacgggtgag acggtttaag     2880

gcggtggcta ggacgacggc ggcgaaaatc atcagcaggg cttgtttaat ccgccacagg     2940

atgtatatag cgattaagaa agctgtgaag cctatccatg tacctagggt cattgcgact     3000

gttgagggtg agagaatttg cttttctaca ggcgatcgcc tgtacaatag ttatctatca     3060

tagaatacta gattctgtgg tgggattcca ccaaagccag ccagctacct atgacgcaac     3120

ccagatatga tgaacaggaa aatatatctc gccacgcagc ggtagatgaa ggatgctgcg     3180

aggatatgga acgaaaatat gggtggaatc ttgtgcgaat tgaggtaacc tcagatccta     3240

tccttgaggt tgattgcgta tttctgggga aaacggagtt tcctcggccc tataatgaaa     3300

ctgataagga ggaagattga tgcgaaaata catcattttt cgggctgata agggtgaacc     3360

tggttggaaa gaacgtaaac tccaacatac ccagggttta actagaattt tggctgaaca     3420

tttcgactct tctgatcaac ctattcctga gttgggatat cgaccgaggg agtttattcg     3480

tattgatggg ttgcataatc ccactgaaca tggctacagc acccattatc gtcagggtga     3540

ctgggaagta actagggtag aaagttactc tcctgatgtg tctatggctg agtttgatat     3600

gatcgtgatt tgcttttgca aatatagccc tgttaatggt cctgtgcaac ctatgccaga     3660

gcgtcgagtg tctgtagatg ggttcgctgg ggatgagaag cggttggagc aatggcacga     3720

gtcccaaaag aaaccgactg aggtttagtt ggtccccggt ttgcttattt gactagccag     3780

attcgccacg acaacagaac cgccagcagg gaagagggga atagaaccgc gaaaagagcg     3840

atcgcattgg tttcgggaat ggcgaaattt ggcccgccat atttgatcag taaggatagc     3900

cctagggata ggatcaggat tttggccacc aataacagtt tgttgtccat ttatatatag     3960

gatgcgatgg gatgcgatcg cctatatttt accaattggt ggcggcctta accccctaag     4020

ccctatgtct gacacccagt caccccaacc gccttagtcg gtattcccca ctgggtgggc     4080

gatacacgat ttcccggaaa aattgaaaaa aaattttgtg gcaggtgata tgatttgcga     4140

tcgaggtggg taagttagaa ctacagaaaa caaataacct catttcaccc cgaggagaac     4200

gaattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg     4260

tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg     4320

atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg     4380

atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag     4440

agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt     4500

ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg     4560

caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa     4620

gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg     4680

aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact     4740

gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa     4800

ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc     4860

agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct     4920

tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca     4980

aggtagtcgg caaataacag ataatctagc agtttaggtt tcagatggag tcagctaggg     5040

tctttaaccc catcgtcaac tataagatgg agagatggca tacggtgaca ctgttaaagc     5100

cataactata ttgctcttgt tgataaaatc agaaactgag gccacttgag agtattgtaa     5160

ataggtagta cagtagtgct acctattttg ttttaaaata actaattgag tttatcgctg     5220

ggactatggt tgataatcag atgtcggaaa agcagaatat tttatcagca gaagctgata     5280

tttacttgca taaaagggat tatcaacaag cagccatttt atatgaacaa gccatcaatc     5340

tcaatcccga ctataaatat aattactgga aactggggtt aaccctgctg ctacaggagc     5400

gggaggaaga agcccagacc acctggctgg tggcgatgag tgatggagac ccagaacaga     5460

ttgaccagtg gacgatagac ttactcgagg tgctatcatc agaagccgat gcacgaacct     5520

ccgccgaaga ctattccgtg gcttggggga ttcgccaaca cattttagaa ataaaccccc     5580

atggtattga taatatatta aagttaattg atttgtcaat taccctgaca acctataccg     5640

gaaatgagat cgcagaatat ggactggttc ccttactgca acaggagtcc atcaatgtca     5700

atccagattt actactgcac ctgtggaaaa aactcctagt tgtagatgca attaatcccc     5760

tatctctgga attgggagag gcggtgatta accatcttgg tgagcaaccg gagtttgtca     5820

ctagcttaat ccctaaatta tacgatattg cctattcctt tggtgaaata ggtatagcca     5880

agggctatgg agagttgttg ttgaacctgg caccgcatca tcaagagttg ctgagggctc     5940

tggctcaatt ttgtattcaa ttaaccgagt atgaccatgg cattgaatat gccagacgtt     6000

cctatcatct cactgaatcc ccagatcagc aagccctgga tagcaatttt atgatcagtg     6060

gtctgatgtc tcaaggggga tgcacaaagc aagtgcgaga ggtggtcaac catcaggaat     6120

cattgctgga tgctatgctg aaagacccgc ccctagactt gggaactggg gttatgcaat     6180

tatataatac cagctttttc tttccttatg ttagagattc acctgctgtc aactcacaaa     6240

taaaagccca agttgctcaa atttgccaag ccaacttaga aacggctcag gcagaaaata     6300

tcgccacata tcaagcccgg aatttacaga aacgccatca atccgcgacc aatcaacccc     6360

taaaaattgg ttatatatct tattgtttcc ggcggcattc tgtgggttgg atatctcgct     6420

ggttatttca acatcacgat cgcgataaat ttgaaattta tgcctatatg attggggcag     6480

aaaatcgaca agatgctctc caaaactggt atgcagaaca agccaacaag tcttatcaat     6540

atggaatagt aagcacagaa gttgccgagc aaatttctga agaccagatt gatattctca     6600

ttgatttaga tagcctgaca ttaaccaata cctgcgccat tatggcactt aaacccgcac     6660

ctgtacaagt cacctggttg ggatgggatg cctctgctat acccaccgtt gactatttta     6720

ttgctgaccc ctatgttttg ccagaaaatg cccaggatta ttactcagaa accatctggc     6780

gactacctca aacctatgtg gccgtcggtg gcttcgaggt cggcattccc agtttgcgaa     6840

gagaggatat caatattccc gaaaatgcca ttgtttattt taccgcccaa agggggccga     6900

aatacaaccc agaactagcc caattgcaaa tgcaaattct gaaacaagta cccaatagtt     6960

atttggtaat taagggattc gataaagagc aatc                                 6994


<210>  2
<211>  5967
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pApl-pilA8005/aadA plasmid including aadA gene flanked by 
       homology arms

<400>  2
gcctgggcct tgagctcgaa tttcctgcat taatgaatcg gccaacgcgc ggggagaggc       60

ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt      120

cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca      180

ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa      240

aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat      300

cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc      360

cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc      420

gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt      480

tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac      540

cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg      600

ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca      660

gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc      720

gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa      780

accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa      840

ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac      900

tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta      960

aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt     1020

taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata     1080

gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc     1140

agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac     1200

cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag     1260

tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac     1320

gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc     1380

agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg     1440

gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc     1500

atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct     1560

gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc     1620

tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc     1680

atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc     1740

agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc     1800

gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca     1860

cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt     1920

tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt     1980

ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca     2040

ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac     2100

ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat     2160

gccaagcttg catgcctgca ggtggcgcga acagaagacg accaacactt tctccctgat     2220

gggttgcgcc ctgatcaatg tcctttaaac cctgatacgt tcttcaaccg tcgaacctag     2280

tccaaccgag tagcccataa ctgaccaccc tatttaatca tgttgcattc tgagcggaac     2340

catgttcaac cccaaactat cccccaagtt ctcccggtcc agtgaatctg gctacaccct     2400

gcttgagtcc atcatggcta tggtggtggt cagcatcctg atggtcggca tttctccggt     2460

gttgggtttt gccttcgcca cacgggtaca ggctcggcgc atagaactag ccagcagagc     2520

cgctaacagt tatatcagtg ctgtccgtac tgacccaacc aataccaact tggtgctgtt     2580

caatcccagt gaccggaacg ctggaccaac tgcaactggt agcttgaact gtggggctgg     2640

tcgagcctgt accaccccag ccaacttgtt ctgtgtggat aatgacggaa gtgggggatg     2700

tgaaaccgat agccccacgg atatgattgt tcagggaatt ggcataaatg agtgtagcga     2760

cgagctagag aatggttatc agttgattgt cagggtgtat cgtgctgacg catttgccca     2820

atcgggacaa ctagaggggg ctaatgatga cgggtctagt aatttagcga gttcccgagt     2880

cggtttagga aaccccagag accccctagt gcaggtgcga accgagatta gccccactag     2940

caccagttcc tttgggagtt tacgtgaccg tttagcagga tgcagttctg acgatgaagg     3000

ggattaatag gtttggtgat ggttcccatg gagtgcaaaa gtaagattat cccggtagag     3060

aggtagttat gctgtcccta gttcggtttt ggttaatttg tgtattgaaa tctaggtcaa     3120

aaaccaccca ggggaatgac ttaggcttca cgatgattga gttattgatt tcggctgttg     3180

tggctagtat gatcatcacg cccatcctat tctttgtggt ggatgtttta cgaaccgata     3240

cccgggaaga aatcacagcc agatcggaac aggagttaca ggcggcagca gatttcattc     3300

gtcgcgacct ctcccaggct atctatatct acgacggtga aggggttaat gcgattagga     3360

gccaacttcc tgattccgat aatgtggtat tggtgttctg gaaacaggag tttcgcgaag     3420

gtgtgattcc tcacggtgac gtccccaggc cagttgctgc taatcattgc gatacgaggc     3480

gttgcgatga tgtccaagtt ttatctttag ttgcttacta tttacaaaca gaaagagagc     3540

ctaatagtcc ttggtctgat caggcgcgta tttctcgctt acaaatcaac gatggtgtca     3600

tcgatccgaa tccagaagat gaatgcaccc aaccaccatg ttatgtcaaa ctgggtgata     3660

aggaattcaa accagaccga ggttttgatc gacctcctgg ccgtacaagc atgaatgctt     3720

gggaagcaaa agagggcgag cagattgaag catcacgaga agttctgatt gattacatag     3780

acaaaacaga actaacaggt gatatagatt gtgaatctcg tggcatcagt aatgatgata     3840

gtgaggattt actatccgtg ggtaagaatt ctgaggggaa tcctatctat ggcttttttg     3900

tatgtgtaga ctcccgtaat acagcagctt tagttaacct gagaatgaat gcggcaattc     3960

gcattgatcc gaatgctaca gaattcgacg aggataggcg atcgcttttt ccagagacca     4020

gctttttggt taaaggctta ggtgttttca attaaccgat aaaatcaggg aatttataat     4080

aaaaggaacc tataaaatga gtattttaat taacccaaaa tcggtgaatc ccaggcgcac     4140

caaaaaccag tcgggcttca ccatcattga gacgttggtg gtagttttaa tgagggaagc     4200

ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct     4260

cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc     4320

acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg     4380

agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg     4440

cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa     4500

gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc     4560

agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc     4620

cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga     4680

ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg     4740

aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc     4800

gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat     4860

acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga     4920

tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata     4980

agtagttata actagccaat tgtaacacaa aaaattaatt atctactgaa tattttacaa     5040

aactttaccc ccaatttcgg aaaacatagt ttagaataca ccaccaaaga tatcacccgt     5100

aacgaggcct tttacgagtg agattagttg tgtatgctgc ggttagctga cctacacgac     5160

atctatcctt gtgacccagg ttaaataatg gaaaatacat taattaagca cttgcttctg     5220

gttcagaaac aggctattaa tttagtgcct gaaaaacctg ttagcagtga ctatcagggt     5280

gcgaaatcgc gaaaatcctc tacagaatca gggttttctt tgttagaagt gttaggggtg     5340

atggtgatta tatcaatact aggtgggata gcggttccga cttggttagg tttcgcgaat     5400

aaccagaaac tgcaaacctc tatccggcga ctcaactggg ctatccactc agccaaaagt     5460

gaggctatac tatctagtac atcatggcaa gcgagtatca gggaatatgg gggacgagta     5520

caggtagcag tccatcccgc gaagactccc cctggtcaac tcccagaagg ttcatggact     5580

aacctagaac cggggataga aattgacaac cgggaaagga acaccaggaa taaatatgaa     5640

actaccctgc gaagagtaga cccggacacc aaccaagtcc gaaacgcggg gacggtgtac     5700

cgagttttat tcaactacca aggctgtccg gtctataacc cgtctaattt atgcacgcaa     5760

acttctctag ctgcgaaggg acgcattgct atccatcatc ctgaagttcc aggtcaaaag     5820

cgatgtatca tcatttcgac gctgataggt gcgactcgca ttggtcagca acagcaaaga     5880

ccggatcaaa atggctacta ttgccattaa ttaataatta ataattaata attaataatt     5940

attaattggg tagcgaccct gtgggcc                                         5967


<210>  3
<211>  8117
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pApl-NS1/Prs-crtW-crtZ plasmid including aadA gene, crtW, crtZ, 
       and homology arms

<400>  3
gcctgggcct tgagctcgaa tttcctgcat taatgaatcg gccaacgcgc ggggagaggc       60

ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt      120

cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca      180

ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa      240

aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat      300

cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc      360

cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc      420

gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt      480

tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac      540

cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg      600

ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca      660

gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc      720

gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa      780

accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa      840

ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac      900

tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta      960

aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt     1020

taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata     1080

gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc     1140

agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac     1200

cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag     1260

tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac     1320

gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc     1380

agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg     1440

gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc     1500

atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct     1560

gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc     1620

tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc     1680

atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc     1740

agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc     1800

gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca     1860

cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt     1920

tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt     1980

ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca     2040

ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac     2100

ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat     2160

gccaagcttg catgcctgca ggtggcgcgc caggtccgaa gaagaacgca ccatagctgc     2220

tggtaaagaa gcccgcgcca gggctgaaca actcctgcaa actgtccgcg ctgagttgga     2280

aaggttgcga ctcgaagctg ataccgtcct acctgcagag gcggagcgag aagccagaga     2340

actgcaagcc agaggtaatg cggccgcttt agcggaaaat tcccgcgcgg cggcggaagt     2400

aaacgacctg attaacgcag tttggcagga aatgggcgac gacgcatcgg aactgtttta     2460

cttacaacaa attgagatgg tcttgcgcga agctgcccaa attccccgcc gggttagtct     2520

ggaatatgtc aatgttattg atagtggaga cggggaagcg atcgctggtt tggttcgcgc     2580

ctatcccgaa attttccgcc aattcctcga aagtgtcgat cgcactttgg gtattaatgt     2640

atctaggact ttgggggaac ataacaagca gaaattaaat aacaattagt caatttgatg     2700

gcaaccaaac acaggtacag gaattatgga aattatcatc gccttattag cagccctggg     2760

tttaggaacc ggagcgactg tatttattat ccgcaatctc tactatattt gtcagcctag     2820

cgaagtgttg atttttgccg gaggtcgtcg ccctacacaa gatgatcgca ctgtgggata     2880

tcgcctggtg aaaggtggta gcagcattcg gacacccctg ctagaacaag cctttcacat     2940

ggatttaacc aatatgatta ttgagttaaa ggtggctaat gcttactcga aaggaggcat     3000

tcctttacag gttgatgggg tggcgaatat taaaattgct ggggaagaac ccacaattca     3060

taatgcgatc gagcgtttgt tggggaaaac tcgccaggaa attgaacgca ttgctaaaga     3120

taccctggaa ggaaacctgc gcggggtatt agccagcctt actcctgaac aggttaatgg     3180

ggataaattg gcttttgcta aaagtctcct cgaagaagct gaggatgact tagaacaact     3240

ggggctgatt ttagatactc tccaaattca aaatatttct gatgacgtgg gatatcttga     3300

ctccatcgga cgacaacaac gggcggaatt gtttagagat tctcgtgtcg ctgaagcaca     3360

agctaaggct gatgctgcta tccgtactgc ggaaaatcat aaaatgactc agttgaaaaa     3420

gttggaaact gaggtagagg tatctaaggc ggaagctgaa cgccgggtgg ctgatgctat     3480

gactaaacgg gcggcggtgg tagcagagtc ggagtcggaa acagcggctg aggtggctag     3540

gactcaggct gaggtctctg ttcaaaaaga aaggattaag caggtagaac agcaactaca     3600

agctgatgtg gtcgcaccag cagaggcgga atgtaaaaag gcgatcgcta gggcgcgggg     3660

ggatgctgcc caaattattg aagatgggaa agcacaagct gaagggacac gacgactggc     3720

tgagtcttgg aaagcagccg gtcggaccgt cactgcataa ttcgtgtcgc tcaaggcgca     3780

ctcccgttct ggataatgtt ttttgcgccg acatcataac ggttctggca aatattctga     3840

aatgagctgt tgacaattaa tcatccggct cgtataatgt gtggaattgt gagcggataa     3900

caatttcata cgctcacaat tggtaccggt gataccagca tcgtcttgat gcccttggca     3960

gcaccctgct aaggaggcaa caagatgacg gctgcggtcg ctgagccgcg aatcgtgcct     4020

cgtcaaacct ggatcgggct cacacttgca gggatgattg tggcaggctg ggggagtctt     4080

cacgtctatg gagtctactt tcatcgctgg gggacaagta gcctcgttat tgtgccagca     4140

atcgtggctg tccagacctg gctcagtgtg ggcctattca ttgtcgcaca cgacgctatg     4200

cacgggtcgc tggcgccggg tcggcctcgc ctaaacgcag ccgtaggcag actcacctta     4260

gggctctatg ccggtttccg atttgatcgc cttaaaacgg cccaccacgc acatcacgcc     4320

gcccccggta ctgccgatga ccctgatttc tacgccccag caccgcgagc gtttttgcct     4380

tggttcctca acttctttcg cacttacttt ggctggcgtg agatggctgt tctgacagct     4440

ttagtcctaa tagccttgtt tggtttgggt gcccggccgg caaatcttct cacgttctgg     4500

gcagctccgg cgctgttaag tgctctacag ctattcactt ttggcacgtg gctgccgcac     4560

cgccacactg atcagccttt tgcggacgcc caccatgctc ggtcttccgg ttacggcccg     4620

gtgctcagcc tgcttacctg ttttcacttt ggtcggcacc acgaacacca tctgacaccg     4680

tggcgcccct ggtggcggct atggcgaggt gagagctaaa cacaggaaac aggaattaga     4740

tatgctgtgg atttggaacg cgctgatcgt gtttgtcaca gtcatcggga tggaagttat     4800

tgccgccttg gcacacaaat acatcatgca cggctggggc tggggatggc atctgtctca     4860

ccacgaaccc cgcaaaggcg ctttcgaagt caatgatctg tatgctgtcg tgtttgcagc     4920

cctgagcatt ttgctcatct acctgggctc gaccggtatg tggccgttgc aatggattgg     4980

tgccggcatg accgcctacg gtctgctgta tttcatggtg cacgatggcc tggtccatca     5040

acggtggcct tttcggtata ttccccgaaa gggctacctg aagcgcctat atatggccca     5100

tcgaatgcat cacgcagtcc gggggaagga agggtgtgtg agttttgggt ttctatatgc     5160

tcccccgttg tcgaaactgc aagcaaccct gcgtgagcgt catggcgctc gcgctggggc     5220

ggcgcgggac gcgcaagggg gcgaagatga accggcatcg gggaaataaa ctagtcatcg     5280

agctagcaag cttggccgga tccggccgga tccggagttt gtagaaacgc aaaaaggcca     5340

tccgtcagga tggccttctg cttaatttga tgcctggcag tttatggcgg gcgtcctgcc     5400

cgccaccctc cgggccgttg cttcgcaacg ttcaaatccg ctcccggcgg atttgtccta     5460

ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga     5520

gcctttcgtt ttatttgatg cctggcagtt ccctactctc gagtcacccc aaccgcctta     5580

gtcggtattc cccactgggt gggcgataca cgatttcccg gaaaaattga aaaaaaattt     5640

tgtggcaggt gatatgattt gcgatcgagg tgggtaagtt agaactacag aaaacaaata     5700

acctcatttc accccgagga gaacgaatta tgagggaagc ggtgatcgcc gaagtatcga     5760

ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg     5820

tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc     5880

tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt     5940

tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg     6000

ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag     6060

aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc     6120

tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg     6180

aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa     6240

cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt     6300

cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact     6360

gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt     6420

atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc     6480

actacgtgaa aggcgagatc accaaggtag tcggcaaata actagctggc ctggaagggt     6540

gggaaagtgt taacaactgg ttgacaatat tccgcttttc caggtcttgt cgaagtacga     6600

ctacgggtat tgatgtggcg acggctgttc aaaattgggg cggcggttct tctcaacctc     6660

aacctacttc cacagctaaa agtctcaaat cttcccgcac taatggtcaa gctgttcgcc     6720

gcactaatgg cgcagcacct aagccagaag atactacaca aagcctacag agcgaggtta     6780

aacaactact tgatcatgtc gcacaaaata ctaagaataa tgctgaggct gaagaggcga     6840

tcgcccaggc tttgcgacag tatcctaacc tcaagcgtcg tttacagatg ctttttaagt     6900

ctcagggaac tcaggcgatc cgaaatatgt ttaatcaccc tactatctac atccctaccg     6960

ctatgctgca aatgtggcta gaacaggatt agagcgtatt cccccccctc tcgtctaggg     7020

tgggggatct gtctctgtaa ctgtatatgc acaaaatggc gtttttgtca ataagccgaa     7080

aaatggaccc cgcttttaac cccaaaactc gtgcatcgcc agttttattt tttggtaaga     7140

taataattat aaccgcgaca aaataagggt tttagccttt atttttggaa atagacacag     7200

taagcccttg ggtgcgattt ccttgagcat accataaccc ccataatctc gtcaactttt     7260

tgcgaggtat ttgccataaa attgtattaa ttcttaacaa tattttaaaa ataaaatcta     7320

tctagccgta taaattagta aaatttatgt aatttcccga ttgagggggt ggggtgtggg     7380

ggggtttata gtcaaattaa tgtgattgga tctcatttaa cctctgacgt actacttgca     7440

tatcctgcca cattaaccat ttaggtttac caacatctcg cgaggaattt ctgagtaggt     7500

atgcagggtg aaaaatcggc atacattgac gaccttccca ctgaaaccac tgaccgcgaa     7560

ttttagtaat cgggcgttta tctcctagaa tagacttaac tgcagtcgcc ccagttagga     7620

ggataatttg aggattaatg aggcggattt gttctaatag ataaggctta caggcttgga     7680

gttctgaagc ggttggggtg cgattttccg ggggacgaca gcggatagca ttagaaatat     7740

aaacttcggt atctgtactc agtcctacgg agtctaaaat attatcaaga agttttccag     7800

atctaccgac aaacggaaga ccttgttcat cttcatgttg tcctggtgct tctccgataa     7860

tcataatggg ggcggtgggg ttccctcgac caatcacagc atgagtgcgg ttttttccaa     7920

gttcacagcg cgagcaccta ttacaatgat cggcaatttg tgcgagagtt tgataggttc     7980

ccggcggtat gggagtcttg gcattttggg gaatttcttc taggtcaaaa tctaccgact     8040

taggggattg taatgggtct tgattaacac taagaatatc gaacaggctg agttgttggg     8100

aattcacata cgcggcc                                                    8117


<210>  4
<211>  8146
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pApl-NS1/aadA-cpcBA plasmid backbone including aadA, cpcA, and 
       cpcB genes with a p15A origin of replication

<400>  4
aggtccgaag aagaacgcac catagctgct ggtaaagaag cccgcgccag ggctgaacaa       60

ctcctgcaaa ctgtccgcgc tgagttggaa aggttgcgac tcgaagctga taccgtccta      120

cctgcagagg cggagcgaga agccagagaa ctgcaagcca gaggtaatgc ggccgcttta      180

gcggaaaatt cccgcgcggc ggcggaagta aacgacctga ttaacgcagt ttggcaggaa      240

atgggcgacg acgcatcgga actgttttac ttacaacaaa ttgagatggt cttgcgcgaa      300

gctgcccaaa ttccccgccg ggttagtctg gaatatgtca atgttattga tagtggagac      360

ggggaagcga tcgctggttt ggttcgcgcc tatcccgaaa ttttccgcca attcctcgaa      420

agtgtcgatc gcactttggg tattaatgta tctaggactt tgggggaaca taacaagcag      480

aaattaaata acaattagtc aatttgatgg caaccaaaca caggtacagg aattatggaa      540

attatcatcg ccttattagc agccctgggt ttaggaaccg gagcgactgt atttattatc      600

cgcaatctct actatatttg tcagcctagc gaagtgttga tttttgccgg aggtcgtcgc      660

cctacacaag atgatcgcac tgtgggatat cgcctggtga aaggtggtag cagcattcgg      720

acacccctgc tagaacaagc ctttcacatg gatttaacca atatgattat tgagttaaag      780

gtggctaatg cttactcgaa aggaggcatt cctttacagg ttgatggggt ggcgaatatt      840

aaaattgctg gggaagaacc cacaattcat aatgcgatcg agcgtttgtt ggggaaaact      900

cgccaggaaa ttgaacgcat tgctaaagat accctggaag gaaacctgcg cggggtatta      960

gccagcctta ctcctgaaca ggttaatggg gataaattgg cttttgctaa aagtctcctc     1020

gaagaagctg aggatgactt agaacaactg gggctgattt tagatactct ccaaattcaa     1080

aatatttctg atgacgtggg atatcttgac tccatcggac gacaacaacg ggcggaattg     1140

tttagagatt ctcgtgtcgc tgaagcacaa gctaaggctg atgctgctat ccgtactgcg     1200

gaaaatcata aaatgactca gttgaaaaag ttggaaactg aggtagaggt atctaaggcg     1260

gaagctgaac gccgggtggc tgatgctatg actaaacggg cggcggtggt agcagagtcg     1320

gagtcggaaa cagcggctga ggtggctagg actcaggctg aggtctctgt tcaaaaagaa     1380

aggattaagc aggtagaaca gcaactacaa gctgatgtgg tcgcaccagc agaggcggaa     1440

tgtaaaaagg cgatcgctag ggcgcggggg gatgctgccc aaattattga agatgggaaa     1500

gcacaagctg aagggacacg acgactggct gagtcttgga aagcagccgg tagtcacccc     1560

aaccgcctta gtcggtattc cccactgggt gggcgataca cgatttcccg gaaaaattga     1620

aaaaaaattt tgtggcaggt gatatgattt gcgatcgagg tgggtaagtt agaactacag     1680

aaaacaaata acctcatttc accccgagga gaacgaatta tgagggaagc ggtgatcgcc     1740

gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg     1800

ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat     1860

attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc     1920

aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa     1980

gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg     2040

caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc     2100

gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt     2160

ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat     2220

gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg     2280

cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc     2340

gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct     2400

agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa     2460

gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata actagctggc     2520

ctggaagggt gggaaagtgt taacaactgg ttgacaatat tccgcttttc caggtcttgt     2580

cgaagtacgc ggaccgttga aggggtgaga aggaaggcga atgttgattt atgaagtttg     2640

attaacattt gtatcaaaat ataaaattct tctcataaac cctgtacaat cttttaagat     2700

ttcggaaagt gttctaggat actgaagaaa tgaaccacgg ggcaattgtt aaaagccttt     2760

gtcgatggtt cgccccggaa ggggtcttag gaggtgacac cgatggattg attgtcgtga     2820

tcattcatgg tgtgtccaat cccaactcaa ctctaagcaa gtcaacaagt aggagataaa     2880

tccatgtttg atgccttcac caaggtggtt tctcaagctg atactcgcgg cgaaatgctg     2940

agtacagctc aaatcgatgc tctgagccaa atggttgctg aaagcaacaa acgtttggat     3000

tctgttaacc gcattaccag caacgcttcc accattgttt ccaacgctgc tcgttctttg     3060

ttcgcagagc aaccccaact gattgctccc ggtggaaacg cctacaccag ccgtcgtatg     3120

gctgcttgct tgcgtgacat ggaaatcatc ctgcgctatg taacctacgc tgtgtttgct     3180

ggcgatgcaa gtgttctcga agatcgttgc ttgaacggtt tgcgtgaaac ttacctggct     3240

ttgggaactc ccggttcttc cgttgctgtc ggtgttggca aaatgaaaga agctgctctg     3300

gcgatcgtta acgatcccgc aggtatcact cctggtgatt gtagcgcttt ggcttcagaa     3360

atcgctggtt actttgaccg tgctgctgca gcagtttcct aatcaagcag atccatagca     3420

tataacaatt gaaacagttt agctgaagtc taagtgactg gacttctgtt tgttacctaa     3480

ttttttgtaa accaatcggg agataactcg agaatgaaaa cccccctaac cgaagcagtt     3540

tctatcgctg attcccaagg tcgtttccta agcagcaccg aaatccaagt agcttttggc     3600

cgttttcgtc aagccaaagc tggtctggaa gctgctaaag ctttgacctc taaagctgat     3660

agtctgatca gtggtgctgc ccaagcagtg tacaacaagt tcccctacac cacccaaatg     3720

cagggaccta actacgcggc agaccaacgc ggtaaggaca aatgtgctcg tgacataggc     3780

tactacctgc ggatggtaac ttattgcctg attgctggtg gaactggccc catggatgag     3840

tacctgattg ccggtattga tgaaatcaac cggactttcg agctttctcc aagctggtac     3900

attgaagccc tgaaatacat caaagctaac cacggtttgt ctggtgacgc tgctgttgaa     3960

gctaactcct acctcgacta cgcgatcaac gccctgagct agactagtca tcgagctagc     4020

aagcttggcc ggatccggcc ggatccggag tttgtagaaa cgcaaaaagg ccatccgtca     4080

ggatggcctt ctgcttaatt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc     4140

ctccgggccg ttgcttcgca acgttcaaat ccgctcccgg cggatttgtc ctactcagga     4200

gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc     4260

gttttatttg atgcctggca gttccctact ctcgactacg ggtattgatg tggcgacggc     4320

tgttcaaaat tggggcggcg gttcttctca acctcaacct acttccacag ctaaaagtct     4380

caaatcttcc cgcactaatg gtcaagctgt tcgccgcact aatggcgcag cacctaagcc     4440

agaagatact acacaaagcc tacagagcga ggttaaacaa ctacttgatc atgtcgcaca     4500

aaatactaag aataatgctg aggctgaaga ggcgatcgcc caggctttgc gacagtatcc     4560

taacctcaag cgtcgtttac agatgctttt taagtctcag ggaactcagg cgatccgaaa     4620

tatgtttaat caccctacta tctacatccc taccgctatg ctgcaaatgt ggctagaaca     4680

ggattagagc gtattccccc ccctctcgtc tagggtgggg gatctgtctc tgtaactgta     4740

tatgcacaaa atggcgtttt tgtcaataag ccgaaaaatg gaccccgctt ttaaccccaa     4800

aactcgtgca tcgccagttt tattttttgg taagataata attataaccg cgacaaaata     4860

agggttttag cctttatttt tggaaataga cacagtaagc ccttgggtgc gatttccttg     4920

agcataccat aacccccata atctcgtcaa ctttttgcga ggtatttgcc ataaaattgt     4980

attaattctt aacaatattt taaaaataaa atctatctag ccgtataaat tagtaaaatt     5040

tatgtaattt cccgattgag ggggtggggt gtgggggggt ttatagtcaa attaatgtga     5100

ttggatctca tttaacctct gacgtactac ttgcatatcc tgccacatta accatttagg     5160

tttaccaaca tctcgcgagg aatttctgag taggtatgca gggtgaaaaa tcggcataca     5220

ttgacgacct tcccactgaa accactgacc gcgaatttta gtaatcgggc gtttatctcc     5280

tagaatagac ttaactgcag tcgccccagt taggaggata atttgaggat taatgaggcg     5340

gatttgttct aatagataag gcttacaggc ttggagttct gaagcggttg gggtgcgatt     5400

ttccggggga cgacagcgga tagcattaga aatataaact tcggtatctg tactcagtcc     5460

tacggagtct aaaatattat caagaagttt tccagatcta ccgacaaacg gaagaccttg     5520

ttcatcttca tgttgtcctg gtgcttctcc gataatcata atgggggcgg tggggttccc     5580

tcgaccaatc acagcatgag tgcggttttt tccaagttca cagcgcgagc acctattaca     5640

atgatcggca atttgtgcga gagtttgata ggttcccggc ggtatgggag tcttggcatt     5700

ttggggaatt tcttctaggt caaaatctac cgacttaggg gattgtaatg ggtcttgatt     5760

aacactaaga atatcgaaca ggctgagttg ttgggaattc acatacgcgg ccgcctgggc     5820

cttgagctcg aatttcctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg     5880

tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg     5940

gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa     6000

cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaatagcgg     6060

agtgtatact ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg     6120

caggagaaaa aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc     6180

ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta     6240

cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg     6300

gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga     6360

cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct     6420

ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg     6480

ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc     6540

caagctggac tgtatgcacg aaccccccat tcagtccgac cgctgcgcct tatccggtaa     6600

ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg     6660

taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga     6720

caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc     6780

agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta     6840

cgcgcagacc aaaacgatct caagaagatc atcttattaa gattatcaaa aaggatcttc     6900

acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa     6960

acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta     7020

tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc     7080

ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat     7140

ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta     7200

tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt     7260

aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt     7320

ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg     7380

ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc     7440

gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc     7500

gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg     7560

cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga     7620

actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta     7680

ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct     7740

tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag     7800

ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga     7860

agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat     7920

aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc     7980

attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg     8040

cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct     8100

tgtctgtaag cggatgccaa gcttgcatgc ctgcaggtgg cgcgcc                    8146


<210>  5
<211>  458
<212>  PRT
<213>  Acinetobacter baylii sp.

<400>  5

Met Arg Pro Leu His Pro Ile Asp Phe Ile Phe Leu Ser Leu Glu Lys 
1               5                   10                  15      


Arg Gln Gln Pro Met His Val Gly Gly Leu Phe Leu Phe Gln Ile Pro 
            20                  25                  30          


Asp Asn Ala Pro Asp Thr Phe Ile Gln Asp Leu Val Asn Asp Ile Arg 
        35                  40                  45              


Ile Ser Lys Ser Ile Pro Val Pro Pro Phe Asn Asn Lys Leu Asn Gly 
    50                  55                  60                  


Leu Phe Trp Asp Glu Asp Glu Glu Phe Asp Leu Asp His His Phe Arg 
65                  70                  75                  80  


His Ile Ala Leu Pro His Pro Gly Arg Ile Arg Glu Leu Leu Ile Tyr 
                85                  90                  95      


Ile Ser Gln Glu His Ser Thr Leu Leu Asp Arg Ala Lys Pro Leu Trp 
            100                 105                 110         


Thr Cys Asn Ile Ile Glu Gly Ile Glu Gly Asn Arg Phe Ala Met Tyr 
        115                 120                 125             


Phe Lys Ile His His Ala Met Val Asp Gly Val Ala Gly Met Arg Leu 
    130                 135                 140                 


Ile Glu Lys Ser Leu Ser His Asp Val Thr Glu Lys Ser Ile Val Pro 
145                 150                 155                 160 


Pro Trp Cys Val Glu Gly Lys Arg Ala Lys Arg Leu Arg Glu Pro Lys 
                165                 170                 175     


Thr Gly Lys Ile Lys Lys Ile Met Ser Gly Ile Lys Ser Gln Leu Gln 
            180                 185                 190         


Ala Thr Pro Thr Val Ile Gln Glu Leu Ser Gln Thr Val Phe Lys Asp 
        195                 200                 205             


Ile Gly Arg Asn Pro Asp His Val Ser Ser Phe Gln Ala Pro Cys Ser 
    210                 215                 220                 


Ile Leu Asn Gln Arg Val Ser Ser Ser Arg Arg Phe Ala Ala Gln Ser 
225                 230                 235                 240 


Phe Asp Leu Asp Arg Phe Arg Asn Ile Ala Lys Ser Leu Asn Val Thr 
                245                 250                 255     


Ile Asn Asp Val Val Leu Ala Val Cys Ser Gly Ala Leu Arg Ala Tyr 
            260                 265                 270         


Leu Met Ser His Asn Ser Leu Pro Ser Lys Pro Leu Ile Ala Met Val 
        275                 280                 285             


Pro Ala Ser Ile Arg Asn Asp Asp Ser Asp Val Ser Asn Arg Ile Thr 
    290                 295                 300                 


Met Ile Leu Ala Asn Leu Ala Thr His Lys Asp Asp Pro Leu Gln Arg 
305                 310                 315                 320 


Leu Glu Ile Ile Arg Arg Ser Val Gln Asn Ser Lys Gln Arg Phe Lys 
                325                 330                 335     


Arg Met Thr Ser Asp Gln Ile Leu Asn Tyr Ser Ala Val Val Tyr Gly 
            340                 345                 350         


Pro Ala Gly Leu Asn Ile Ile Ser Gly Met Met Pro Lys Arg Gln Ala 
        355                 360                 365             


Phe Asn Leu Val Ile Ser Asn Val Pro Gly Pro Arg Glu Pro Leu Tyr 
    370                 375                 380                 


Trp Asn Gly Ala Lys Leu Asp Ala Leu Tyr Pro Ala Ser Ile Val Leu 
385                 390                 395                 400 


Asp Gly Gln Ala Leu Asn Ile Thr Met Thr Ser Tyr Leu Asp Lys Leu 
                405                 410                 415     


Glu Val Gly Leu Ile Ala Cys Arg Asn Ala Leu Pro Arg Met Gln Asn 
            420                 425                 430         


Leu Leu Thr His Leu Glu Glu Glu Ile Gln Leu Phe Glu Gly Val Ile 
        435                 440                 445             


Ala Lys Gln Glu Asp Ile Lys Thr Ala Asn 
    450                 455             


<210>  6
<211>  446
<212>  PRT
<213>  Streptomyces coelicolor

<400>  6

Met Thr Pro Asp Pro Leu Ala Pro Leu Asp Leu Ala Phe Trp Asn Ile 
1               5                   10                  15      


Glu Ser Ala Glu His Pro Met His Leu Gly Ala Leu Gly Val Phe Glu 
            20                  25                  30          


Ala Asp Ser Pro Thr Ala Gly Ala Leu Ala Ala Asp Leu Leu Ala Ala 
        35                  40                  45              


Arg Ala Pro Ala Val Pro Gly Leu Arg Met Arg Ile Arg Asp Thr Trp 
    50                  55                  60                  


Gln Pro Pro Met Ala Leu Arg Arg Pro Phe Ala Phe Gly Gly Ala Thr 
65                  70                  75                  80  


Arg Glu Pro Asp Pro Arg Phe Asp Pro Leu Asp His Val Arg Leu His 
                85                  90                  95      


Ala Pro Ala Thr Asp Phe His Ala Arg Ala Gly Arg Leu Met Glu Arg 
            100                 105                 110         


Pro Leu Glu Arg Gly Arg Pro Pro Trp Glu Ala His Val Leu Pro Gly 
        115                 120                 125             


Ala Asp Gly Gly Ser Phe Ala Val Leu Phe Lys Phe His His Ala Leu 
    130                 135                 140                 


Ala Asp Gly Leu Arg Ala Leu Thr Leu Ala Ala Gly Val Leu Asp Pro 
145                 150                 155                 160 


Met Asp Leu Pro Ala Pro Arg Pro Arg Pro Glu Gln Pro Pro Arg Gly 
                165                 170                 175     


Leu Leu Pro Asp Val Arg Ala Leu Pro Asp Arg Leu Arg Gly Ala Leu 
            180                 185                 190         


Ser Asp Ala Gly Arg Ala Leu Asp Ile Gly Ala Ala Ala Ala Leu Ser 
        195                 200                 205             


Thr Leu Asp Val Arg Ser Ser Pro Ala Leu Thr Ala Ala Ser Ser Gly 
    210                 215                 220                 


Thr Arg Arg Thr Ala Gly Val Ser Val Asp Leu Asp Asp Val His His 
225                 230                 235                 240 


Val Arg Lys Thr Thr Gly Gly Thr Val Asn Asp Val Leu Ile Ala Val 
                245                 250                 255     


Val Ala Gly Ala Leu Arg Arg Trp Leu Asp Glu Arg Gly Asp Gly Ser 
            260                 265                 270         


Glu Gly Val Ala Pro Arg Ala Leu Ile Pro Val Ser Arg Arg Arg Pro 
        275                 280                 285             


Arg Ser Ala His Pro Gln Gly Asn Arg Leu Ser Gly Tyr Leu Met Arg 
    290                 295                 300                 


Leu Pro Val Gly Asp Pro Asp Pro Leu Ala Arg Leu Gly Thr Val Arg 
305                 310                 315                 320 


Ala Ala Met Asp Arg Asn Lys Asp Ala Gly Pro Gly Arg Gly Ala Gly 
                325                 330                 335     


Ala Val Ala Leu Leu Ala Asp His Val Pro Ala Leu Gly His Arg Leu 
            340                 345                 350         


Gly Gly Pro Leu Val Ser Gly Ala Ala Arg Leu Trp Phe Asp Leu Leu 
        355                 360                 365             


Val Thr Ser Val Pro Leu Pro Ser Leu Gly Leu Arg Leu Gly Gly His 
    370                 375                 380                 


Pro Leu Thr Glu Val Tyr Pro Leu Ala Pro Leu Ala Arg Gly His Ser 
385                 390                 395                 400 


Leu Ala Val Ala Val Ser Thr Tyr Arg Gly Arg Val His Tyr Gly Leu 
                405                 410                 415     


Leu Ala Asp Ala Lys Ala Val Pro Asp Leu Asp Arg Leu Ala Val Ala 
            420                 425                 430         


Val Ala Glu Glu Val Glu Thr Leu Leu Thr Ala Cys Arg Pro 
        435                 440                 445     


<210>  7
<211>  457
<212>  PRT
<213>  Alcanivorax borkumensis

<400>  7

Met Lys Ala Leu Ser Pro Val Asp Gln Leu Phe Leu Trp Leu Glu Lys 
1               5                   10                  15      


Arg Gln Gln Pro Met His Val Gly Gly Leu Gln Leu Phe Ser Phe Pro 
            20                  25                  30          


Glu Gly Ala Gly Pro Lys Tyr Val Ser Glu Leu Ala Gln Gln Met Arg 
        35                  40                  45              


Asp Tyr Cys His Pro Val Ala Pro Phe Asn Gln Arg Leu Thr Arg Arg 
    50                  55                  60                  


Leu Gly Gln Tyr Tyr Trp Thr Arg Asp Lys Gln Phe Asp Ile Asp His 
65                  70                  75                  80  


His Phe Arg His Glu Ala Leu Pro Lys Pro Gly Arg Ile Arg Glu Leu 
                85                  90                  95      


Leu Ser Leu Val Ser Ala Glu His Ser Asn Leu Leu Asp Arg Glu Arg 
            100                 105                 110         


Pro Met Trp Glu Ala His Leu Ile Glu Gly Ile Arg Gly Arg Gln Phe 
        115                 120                 125             


Ala Leu Tyr Tyr Lys Ile His His Ser Val Met Asp Gly Ile Ser Ala 
    130                 135                 140                 


Met Arg Ile Ala Ser Lys Thr Leu Ser Thr Asp Pro Ser Glu Arg Glu 
145                 150                 155                 160 


Met Ala Pro Ala Trp Ala Phe Asn Thr Lys Lys Arg Ser Arg Ser Leu 
                165                 170                 175     


Pro Ser Asn Pro Val Asp Met Ala Ser Ser Met Ala Arg Leu Thr Ala 
            180                 185                 190         


Ser Ile Ser Lys Gln Ala Ala Thr Val Pro Gly Leu Ala Arg Glu Val 
        195                 200                 205             


Tyr Lys Val Thr Gln Lys Ala Lys Lys Asp Glu Asn Tyr Val Ser Ile 
    210                 215                 220                 


Phe Gln Ala Pro Asp Thr Ile Leu Asn Asn Thr Ile Thr Gly Ser Arg 
225                 230                 235                 240 


Arg Phe Ala Ala Gln Ser Phe Pro Leu Pro Arg Leu Lys Val Ile Ala 
                245                 250                 255     


Lys Ala Tyr Asn Cys Thr Ile Asn Thr Val Val Leu Ser Met Cys Gly 
            260                 265                 270         


His Ala Leu Arg Glu Tyr Leu Ile Ser Gln His Ala Leu Pro Asp Glu 
        275                 280                 285             


Pro Leu Ile Ala Met Val Pro Met Ser Leu Arg Gln Asp Asp Ser Thr 
    290                 295                 300                 


Gly Gly Asn Gln Ile Gly Met Ile Leu Ala Asn Leu Gly Thr His Ile 
305                 310                 315                 320 


Cys Asp Pro Ala Asn Arg Leu Arg Val Ile His Asp Ser Val Glu Glu 
                325                 330                 335     


Ala Lys Ser Arg Phe Ser Gln Met Ser Pro Glu Glu Ile Leu Asn Phe 
            340                 345                 350         


Thr Ala Leu Thr Met Ala Pro Thr Gly Leu Asn Leu Leu Thr Gly Leu 
        355                 360                 365             


Ala Pro Lys Trp Arg Ala Phe Asn Val Val Ile Ser Asn Ile Pro Gly 
    370                 375                 380                 


Pro Lys Glu Pro Leu Tyr Trp Asn Gly Ala Gln Leu Gln Gly Val Tyr 
385                 390                 395                 400 


Pro Val Ser Ile Ala Leu Asp Arg Ile Ala Leu Asn Ile Thr Leu Thr 
                405                 410                 415     


Ser Tyr Val Asp Gln Met Glu Phe Gly Leu Ile Ala Cys Arg Arg Thr 
            420                 425                 430         


Leu Pro Ser Met Gln Arg Leu Leu Asp Tyr Leu Glu Gln Ser Ile Arg 
        435                 440                 445             


Glu Leu Glu Ile Gly Ala Gly Ile Lys 
    450                 455         


<210>  8
<211>  2595
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized S. cerevisiae phosphatidate phosphatase (PAH1)

<400>  8
atggaattcc aatatgttgg tcgggctttg ggtagtgtta gtaaaacgtg gtcgagtatc       60

aaccccgcca ccctgagcgg cgctatcgat gtcattgtcg tggaacaccc cgatggccgg      120

ctcagttgta gccccttcca tgtgcgcttt ggtaaattcc agattctgaa acccagccaa      180

aagaaagtcc aggtctttat taacgagaaa ctgtcgaata tgcccatgaa actctcggat      240

agcggcgagg cgtacttcgt ttttgagatg ggtgatcaag tgacggatgt cccggatgaa      300

ctgctcgtct cgccggtcat gagtgccacg agtagtccgc cccaatcgcc ggaaacctcg      360

attctcgaag gcggtaccga aggcgagggc gaaggtgaga atgaaaataa gaaaaaggaa      420

aagaaggtgt tggaggagcc cgactttctg gacattaatg acaccggtga cagcggcagc      480

aagaacagtg agacgacggg ttcgctctcg ccgaccgaaa gtagtacgac gacgccgccc      540

gatagcgtcg aggaacgcaa gttggtcgaa caacggacca agaattttca gcaaaagctg      600

aataagaaac tgaccgaaat ccatattccg agcaaattgg acaataacgg tgatttgctc      660

ctggacaccg agggttataa gccgaataaa aacatgatgc acgacacgga tattcagctg      720

aagcaattgc tcaaggatga gttcggtaac gatagcgata tttcgagctt catcaaagaa      780

gacaagaatg gcaacattaa aatcgtgaac ccctatgagc atttgaccga tttgagtccc      840

ccgggtacgc ccccgaccat ggccacgagt ggcagtgtcc tgggcttgga tgcgatggag      900

agtggttcga cgctgaacag cttgagcagc agcccgagcg gcagtgacac cgaggatgag      960

acgagcttta gcaaggaaca gtcgtcgaag agtgaaaaaa cgtcgaagaa aggcaccgcg     1020

ggttcgggtg aaacggagaa acgctacatc cgcacgatcc ggctcacgaa tgatcagctg     1080

aaatgcctca acttgacgta cggtgaaaat gacttgaaat ttagtgttga ccatggcaaa     1140

gccattgtga ccagcaaatt gtttgtctgg cgctgggacg tccccatcgt tatcagcgac     1200

attgacggta cgattacgaa aagtgatgcg ctgggccacg tcctcgccat gatcggcaaa     1260

gattggaccc atctcggcgt cgctaagctg ttcagtgaga tctcgcgcaa cggttacaat     1320

atcctgtacc tgaccgcgcg ctcggccggt caggctgaca gtacccgctc gtatctccgc     1380

agtattgagc agaacggtag caagctcccg aacggccccg tcattctgag ccccgatcgg     1440

accatggctg ccctgcgccg ggaggtgatt ctgaaaaagc ccgaagtctt taaaatcgct     1500

tgcttgaacg atatccgctc gctctatttc gaagactcgg ataacgaagt ggacacggag     1560

gaaaagagca cgccgttttt cgcgggcttt ggcaatcgga tcaccgatgc gctcagctat     1620

cggacggtcg gcatcccgag tagccgcatc ttcacgatta acacggaagg cgaggtgcac     1680

atggagctgc tcgagctcgc cggttaccgg agtagctata tccatatcaa cgaactggtc     1740

gatcacttct tcccgccggt gagcctggac tcggtcgatc tgcgcacgaa cacgagcatg     1800

gtcccgggca gcccgccgaa ccgcaccctg gataactttg atagcgaaat caccagtggc     1860

cgcaagacgt tgtttcgcgg taatcaggag gaaaaattca cggacgtcaa cttttggcgc     1920

gatccgttgg tggacatcga caacctctcg gatatcagta acgatgattc ggacaatatt     1980

gatgaagaca ccgatgtgag ccaacagtcg aacatcagcc gcaaccgcgc taactcggtc     2040

aagacggcca aggtgaccaa ggctccgcag cggaatgtgt cgggcagtac gaataacaat     2100

gaagttctgg ctgcgagtag tgatgttgaa aatgccagtg acttggttag cagccactcg     2160

agtagcggct cgacccccaa caagtcgacg atgagtaagg gtgatatcgg caaacaaatc     2220

tatctggaac tgggctcgcc cttggcgagt cccaaactcc ggtatctgga cgatatggat     2280

gatgaggact cgaactataa tcgcaccaag agccgccggg ctagtagcgc cgctgctacc     2340

agcatcgaca aggagtttaa aaagctcagt gtgagtaaag ctggcgctcc cacccgcatc     2400

gttagcaaga tcaacgtgtc gaatgatgtg cacagtttgg gcaacagtga taccgaaagc     2460

cggcgggaac agagcgtcaa tgaaaccggt cgcaatcagt tgccgcacaa tagtatggat     2520

gataaggatt tggattcgcg ggtgagtgac gagttcgatg acgatgagtt tgatgaagat     2580

gagtttgagg attag                                                      2595


<210>  9
<211>  6936
<212>  DNA
<213>  Triticum aestivum

<400>  9
atgggatcca cacatttgcc cattgtcggc cttaatgcct cgacaacacc atcgctatcc       60

actattcgcc cggtaaattc agccggtgct gcattccaac catctgcccc ttctagaacc      120

tccaagaaga aaagtcgtcg tgttcagtca ttaagggatg gaggcgatgg aggcgtgtca      180

gaccctaacc agtctattcg ccaaggtctt gccggcatca ttgacctccc aaaggagggc      240

acatcagctc cggaagtgga tatttcacat gggtccgaag aacccagggg ctcctaccaa      300

atgaatggga tactgaatga agcacataat gggaggcatg cttcgctgtc taaggttgtc      360

gaattttgta tggcattggg cggcaaaaca ccaattcaca gtgtattagt tgcgaacaat      420

ggaagggcag cagctaagtt catgcggagt gtccgaacat gggctaatga aacatttggg      480

tcagagaagg caattcagtt gatagctatg gctactccag aagacatgag gataaatgca      540

gagcacatta gaattgctga tcaatttgtt gaagtacccg gtggaacaaa caataacaac      600

tatgcaaatg tccaactcat agtggagata gcagtgagaa ccggtgtttc tgctgtttgg      660

cctggttggg gccatgcatc tgagaatcct gaacttccag atgcactaaa tgcaaacgga      720

attgtttttc ttgggccacc atcatcatca atgaacgcac taggtgacaa ggttggttca      780

gctctcattg ctcaagcagc aggggttccg actcttcctt ggggtggatc acaggtggaa      840

attccattag aagtttgttt ggactcgata cctgcggaga tgtataggaa agcttgtgtt      900

agtactacgg aggaagcact tgcgagttgt cagatgattg ggtatccagc catgattaaa      960

gcatcatggg gtggtggtgg taaagggatc cgaaaggtta ataacgacga tgatgtcaga     1020

gcactgttta agcaagtgca aggtgaagtt cctggctccc caatatttat catgagactt     1080

gcatctcaga gtcgacatct tgaagttcag ttgctttgtg atcaatatgg caatgtagct     1140

gcgcttcaca gtcgtgactg cagtgtgcaa cggcgacacc aaaagattat tgaggaagga     1200

ccagttactg ttgctcctcg cgagacagtg aaagagctag agcaagcagc aaggaggctt     1260

gctaaggctg tgggttatgt tggtgctgct actgttgaat atctctacag catggagact     1320

ggtgaatact attttctgga acttaatcca cggttgcagg ttgagcatcc agtcaccgag     1380

tggatagctg aagtaaactt gcctgcagct caagttgcag ttggaatggg tatacccctt     1440

tggcaggttc cagagatcag acgtttctat ggaatggaca atggaggagg ctatgacatt     1500

tggagggaaa cagcagctct tgctactcca tttaacttcg atgaagtgga ttctcaatgg     1560

ccaaagggtc attgtgtagc agttaggata accagtgagg atccagatga cggattcaag     1620

cctaccggtg gaaaagtaaa ggagatcagt tttaaaagca agccaaatgt ttgggcctat     1680

ttctctgtta agtccggtgg aggcattcat gaatttgctg attctcagtt tggacatgtt     1740

tttgcatatg gagtgtctag agcagcagca ataaccaaca tgtctcttgc gctaaaagag     1800

attcaaattc gtggagaaat tcattcaaat gttgattaca cagttgatct cttgaatgcc     1860

tcagacttca aagaaaacag gattcatact ggctggctgg ataacagaat agcaatgcga     1920

gtccaagctg agagacctcc gtggtatatt tcagtggttg gaggagctct atataaaaca     1980

ataacgagca acacagacac tgtttctgaa tatgttagct atctcgtcaa gggtcagatt     2040

ccaccgaagc atatatccct tgtccattca actgtttctt tgaatataga ggaaagcaaa     2100

tatacaattg aaactataag gagcggacag ggtagctaca gattgcgaat gaatggatca     2160

gttattgaag caaatgtcca aacattatgt gatggtggac ttttaatgca gttggatgga     2220

aacagccatg taatttatgc tgaagaagag gccggtggta cacggcttct aattgatgga     2280

aagacatact tgttacagaa tgatcacgat ccttcaaggt tattagctga gacaccctgc     2340

aaacttcttc gtttcttggt tgccgatggt gctcatgttg aagctgatgt accatatgcg     2400

gaagttgagg ttatgaagat gtgcatgccc ctcttgtcac ctgctgctgg tgtcattaat     2460

gttttgttgt ctgagggcca gcctatgcag gctggtgatc ttatagcaag acttgatctt     2520

gatgaccctt ctgctgtgaa gagagctgag ccatttaacg gatctttccc agaaatgagc     2580

cttcctattg ctgcttctgg ccaagttcac aaaagatgtg ccacaagctt gaatgctgct     2640

cggatggtcc ttgcaggata tgatcacccg atcaacaaag ttgtacaaga tctggtatcc     2700

tgtctagatg ctcctgagct tcctttccta caatgggaag agcttatgtc tgttttagca     2760

actagacttc caaggcttct taagagcgag ttggagggta aatacagtga atataagtta     2820

aatgttggcc atgggaagag caaggatttc ccttccaaga tgctaagaga gataatcgag     2880

gaaaatcttg cacatggttc tgagaaggaa attgctacaa atgagaggct tgttgagcct     2940

cttatgagcc tactgaagtc atatgagggt ggcagagaaa gccatgcaca ctttattgtg     3000

aagtcccttt tcgaggacta tctctcggtt gaggaactat tcagtgatgg cattcagtct     3060

gatgtgattg aacgcctgcg ccaacaacat agtaaagatc tccagaaggt tgtagacatt     3120

gtgttgtctc accagggtgt gagaaacaaa actaagctga tactaacact catggagaaa     3180

ctggtctatc caaaccctgc tgtctacaag gatcagttga ctcgcttttc ctccctcaat     3240

cacaaaagat attataagtt ggcccttaaa gctagcgagc ttcttgaaca aaccaagctt     3300

agtgagctcc gcacaagcat tgcaaggagc ctttcagaac ttgagatgtt tactgaagaa     3360

aggacggcca ttagtgagat catgggagat ttagtgactg ccccactgcc agttgaagat     3420

gcactggttt ctttgtttga ttgtagtgat caaactcttc agcagagggt gatcgagacg     3480

tacatatctc gattatacca gcctcatctt gtcaaggata gtatccagct gaaatatcag     3540

gaatctggtg ttattgcttt atgggaattc gctgaagcgc attcagagaa gagattgggt     3600

gctatggtta ttgtgaagtc gttagaatct gtatcagcag caattggagc tgcactaaag     3660

ggtacatcac gctatgcaag ctctgagggt aacataatgc atattgcttt attgggtgct     3720

gataatcaaa tgcatggaac tgaagacagt ggtgataacg atcaagctca agtcaggata     3780

gacaaacttt ctgcgacact ggaacaaaat actgtcacag ctgatctccg tgctgctggt     3840

gtgaaggtta ttagttgcat tgttcaaagg gatggagcac tcatgcctat gcgccatacc     3900

ttcctcttgt cggatgaaaa gctttgttat ggggaagagc cggttctccg gcatgtggag     3960

cctcctcttt ctgctcttct tgagttgggt aagttgaaag tgaaaggata caatgaggtg     4020

aagtatacac cgtcacgtga tcgtcagtgg aacatataca cacttagaaa tacagagaac     4080

cccaaaatgt tgcacagggt gtttttccga actcttgtca ggcaacccgg tgcttccaac     4140

aaattcacat caggcaacat cagtgatgtt gaagtgggag gagctgagga atctctttca     4200

tttacatcga gcagcatatt aagatcgctg atgactgcta tagaagagtt ggagcttcac     4260

gcgattagga caggtcactc tcatatgttt ttgtgcatat tgaaagagcg aaagcttctt     4320

gatcttgttc ccgtttcagg gaacaaagtt gtggatattg gccaagatga agctactgca     4380

tgcttgcttc tgaaagaaat ggctctacag atacatgaac ttgtgggtgc aaggatgcat     4440

catctttctg tatgccaatg ggaggtgaaa cttaagttgg acagcgatgg gcctgccagt     4500

ggtacctgga gagttgtaac aaccaatgtt actagtcaca cctgcactgt ggatatctac     4560

cgtgaggttg aagatacaga atcacagaaa ctagtatacc actctgctcc atcgtcatct     4620

ggtcctttgc atggcgttgc actgaatact ccatatcagc ctttgagtgt tattgatctg     4680

aaacgttgct ccgctagaaa caacagaact acatactgct atgattttcc gttggcattt     4740

gaaactgcag tgcagaagtc atggtctaac atttctagtg acaataaccg atgttatgtt     4800

aaagcaacgg agctggtgtt tgctcacaag aatgggtcat ggggcactcc tgtaattcct     4860

atggagcgtc ctgctgggct caatgacatt ggtatggtag cttggatctt ggacatgtcc     4920

actcctgaat atcccaatgg caggcagatt gttgtcatcg caaatgatat tacttttaga     4980

gctggatcgt ttggtccaag ggaagatgca ttttttgaaa ctgttaccaa cctagcttgt     5040

gagaggaggc ttcctctcat ctacttggca gcaaactctg gtgctcggat cggcatagca     5100

gatgaagtaa aatcttgctt ccgtgttgga tggtctgatg atggcagccc tgaacgtggg     5160

tttcaatata tttatctgac tgaagaagac catgctcgta ttagcgcttc tgttatagcg     5220

cacaagatgc agcttgataa tggtgaaatt aggtgggtta ttgattctgt tgtagggaag     5280

gaggatgggc taggtgtgga gaacatacat ggaagtgctg ctattgccag tgcctattct     5340

agggcctatg aggagacatt tacgcttaca tttgtgactg gaaggactgt tggaatagga     5400

gcatatcttg ctcgacttgg catacggtgc attcagcgta ctgaccagcc cattatccta     5460

actgggtttt ctgccttgaa caagcttctt ggccgggaag tgtacagctc ccacatgcag     5520

ttgggtggcc ccaaaattat ggcgacaaac ggtgttgtcc atctgacagt ttcagatgac     5580

cttgaaggtg tatctaatat attgaggtgg ctcagctatg ttcctgccaa cattggtgga     5640

cctcttccta ttacaaaatc tttggaccca cctgacagac ccgttgctta catccctgag     5700

aatacatgcg atcctcgtgc tgccatcagt ggcattgatg atagccaagg gaaatggttg     5760

gggggcatgt tcgacaaaga cagttttgtg gagacatttg aaggatgggc gaagtcagtt     5820

gttactggca gagcgaaact cggagggatt ccggtgggtg ttatagctgt ggagacacag     5880

actatgatgc agctcatccc tgctgatcca ggccagcttg attcccatga gcgatctgtt     5940

cctcgtgctg ggcaagtctg gtttccagat tcagctacta agacagcgca ggcaatgctg     6000

gacttcaacc gtgaaggatt acctctgttc atccttgcta actggagagg cttctctggt     6060

ggacaaagag atctttttga aggaatcctt caggctgggt caacaattgt tgagaacctt     6120

agggcataca atcagcctgc ctttgtatat atccccaagg ctgcagagct acgtggaggg     6180

gcttgggtcg tgattgatag caagataaat ccagatcgca ttgagttcta tgctgagagg     6240

actgcaaagg gcaatgttct cgaacctcaa gggttgatcg agatcaagtt caggtcagag     6300

gaactccaag agtgcatggg taggcttgat ccagaattga taaatctgaa ggcaaagctc     6360

cagggagtaa agcatgaaaa tggaagtcta cctgagtcag aatcccttca gaagagcata     6420

gaagcccgga agaaacagtt gttgcctttg tatactcaaa ttgcggtacg gttcgctgaa     6480

ttgcatgaca cttcccttag aatggctgct aagggtgtga ttaagaaggt tgtagactgg     6540

gaagattcta ggtcgttctt ctacaagaga ttacggagga ggatatccga ggatgttctt     6600

gcgaaggaaa ttagaggtgt aagtggcaag cagttttctc accaatcggc aatcgagctg     6660

atccagaaat ggtacttggc ctctaaggga gctgaaacag gaagcactga atgggatgat     6720

gacgatgctt ttgttgcctg gagggaaaac cctgaaaact accaggagta tatcaaagaa     6780

cccagggctc aaagggtatc tcagttgctc tcagatgttg cagactccag tccagatcta     6840

gaagccttgc cacagggtct ttctatgcta ctagagaaga tggatcctgc aaagagggaa     6900

attgttgaag actttgaaat aaaccttgta aagtaa                               6936


<210>  10
<211>  996
<212>  DNA
<213>  Synechococcus PCC7942

<400>  10
atgtcagagg ctcaaacgcc cctgacagta ccgaagaagt ttcttggtgc tccaggaggc       60

ttcaacccca ccgtcgcact cttcttggca ggttatacct gcgcggcgct ctcagttttg      120

gggtactggt gctggagttg gccccactgg ctatctttcc ttctgagtgt cacagcctta      180

catttggtag gcaccgtcat tcacgatgcc tctcataatg tggctcacgc cagtcgcatt      240

ctgaatgcga ttttgggaca tggcagtgca ctattgctgg gctttacttt tccggtgttt      300

acgcgggttc acctgcaaca tcacgcccac gtcaacgatc ccaagaacga tcccgaccac      360

atcgtttcca cctttgggcc gctgtggttg atcgcaccgc gcttcttcta tcacgagatc      420

tatttcttcc agcgccgcct ttggaagaaa tttgaattac tcgaatggtt cctcagtcgc      480

gctgtggtca tcggcatctt tgcctgcggc gtcaagtttg gcttcctggg cttcctgatg      540

aactactggc tggctccagc cttggtcgtt ggcattgccc taggactctt cttcgactat      600

ttaccccacc gccccttcca agagcgcaac cgctggcgca atgcacgggt ctatcccggt      660

caggtgatga acatcctgat catgggtcag aactatcacc tgatccatca cctctggcca      720

tcgatcccct ggtatctcta ccgaccggcc taccacgcta ccaagccgtt gttggaccta      780

cgccagtcgc cgcaaacgct cgggattctc tccagcaaaa aagatttctg gaactttatc      840

tacgacgttt tcatcggcat ccgcattcac caatcgcacg aggctgagcc gcagagctcc      900

gtcgttcctg aaacgaagtc gagtgaatca gccgttctcg caaaagctcc gatgtctgcc      960

acagaagact ctcgtgagcc agccttgacg aagtag                                996


<210>  11
<211>  486
<212>  DNA
<213>  Brevundimonas sp. SD212

<400>  11
atggcctggc tgacgtggat cgcgctgttc ctgaccgcct ttttgggcat ggaggcgttc       60

gcctggatca tgcaccgcta tgtgatgcac ggtttcctgt ggtcctggca ccgcagccat      120

catgagccgc acgatcaccc cctggagaag aacgacctgt tcgccgtggt cttcgccgcc      180

ccggccatcg tcatggtggc cgtgggtctg cacctgtggc cctgggccct gccggtcggc      240

ctggggatca cggcctatgg gatggtctat ttcttcttcc acgacggcct ggtgcaccgg      300

cggttcccga cgggcttttc cgggcggtcc ggcttctgga cgcggcgcat ccaggcgcac      360

cgtctgcatc acgccgtgcg cacgcgcgaa ggctgcgtct ccttcggctt tctgtgggtg      420

cggtcggcgc gggcgctgaa ggccgaactg gctcagaagc ggggctcttc cagcagcggc      480

gcctga                                                                 486


<210>  12
<211>  735
<212>  DNA
<213>  Brevundimonas sp. SD212

<400>  12
atgaccgccg ccgtcgccga gccccgcatc gtcccgcgcc agacctggat cggtctgacc       60

ctggcgggaa tgatcgtggc gggatggggg agcctgcacg tctacggcgt ctattttcac      120

cgctggggca cctccagtct ggtgatcgtc ccggcgatcg tagcggtcca gacctggttg      180

tcggtcggcc ttttcatcgt cgcccatgac gccatgcacg gctccctggc gccgggacgg      240

ccgcggctga acgccgcagt cggccggctg accctggggc tctatgcggg cttccgcttc      300

gatcggctga agacggcgca ccacgcccac cacgccgcgc ccggcacggc cgacgacccg      360

gacttttacg ccccggcgcc ccgcgccttc cttccctggt tcctgaactt ctttcgcacc      420

tatttcggct ggcgcgagat ggcggtcctg accgccctgg tcctgatcgc cctcttcggc      480

ctgggggcgc ggccggccaa tctcctgacc ttctgggccg cgccggccct gctttcagcg      540

cttcagctct tcaccttcgg cacctggctg ccgcaccgcc acaccgacca gccgttcgcc      600

gacgcccacc acgcccgcag cagcggctac ggccccgttc tttccctgct cacctgcttc      660

cacttcggcc gccaccacga acaccacctc accccctggc ggccctggtg gcgtttgtgg      720

cgcggcgagt cttga                                                       735


<210>  13
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer to A. plantensis genome outside of homology arm

<400>  13
actactaatg tggctttcca ggg                                               23


<210>  14
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer to A. plantensis outside of the homology arm

<400>  14
gtaaattagc gcgatgggtt tg                                                22


<210>  15
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer to homology arm upstream of pilA gene

<400>  15
gttggagcaa tggcacgagt c                                                 21


<210>  16
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer to upstream end of aadA gene

<400>  16
atgacgccaa ctacctctga tag                                               23


<210>  17
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer to downstream end of aadA gene

<400>  17
gtatcagccc gtcatacttg aa                                                22


<210>  18
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer to pilA gene

<400>  18
gacggtggtt tcaccttaat cg                                                22


<210>  19
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer to pilA gene

<400>  19
tgctgtagta cctacccaga cc                                                22


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  20
cataccctgc tgtgtggcaa                                                   20


<210>  21
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  21
cgggtgatat ctttggtggt g                                                 21


<210>  22
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  22
gtacaaatgt acggccagca ac                                                22


<210>  23
<211>  528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon-optomized Pantoea ananatis beta-carotene 3,3'-hydroxylase 
       (crtZ)

<400>  23
atgctgtgga tttggaacgc gctgatcgtg tttgtcacag tcatcgggat ggaagttatt       60

gccgccttgg cacacaaata catcatgcac ggctggggct ggggatggca tctgtctcac      120

cacgaacccc gcaaaggcgc tttcgaagtc aatgatctgt atgctgtcgt gtttgcagcc      180

ctgagcattt tgctcatcta cctgggctcg accggtatgt ggccgttgca atggattggt      240

gccggcatga ccgcctacgg tctgctgtat ttcatggtgc acgatggcct ggtccatcaa      300

cggtggcctt ttcggtatat tccccgaaag ggctacctga agcgcctata tatggcccat      360

cgaatgcatc acgcagtccg ggggaaggaa gggtgtgtga gttttgggtt tctatatgct      420

cccccgttgt cgaaactgca agcaaccctg cgtgagcgtc atggcgctcg cgctggggcg      480

gcgcgggacg cgcaaggggg cgaagatgaa ccggcatcgg ggaaataa                   528


<210>  24
<211>  720
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon-optomized Brevundimonas sp. SD212 beta-carotene 
       4,4'-ketolase (crtW)

<400>  24
atgacggctg cggtcgctga gccgcgaatc gtgcctcgtc aaacctggat cgggctcaca       60

cttgcaggga tgattgtggc aggctggggg agtcttcacg tctatggagt ctactttcat      120

cgctggggga caagtagcct cgttattgtg ccagcaatcg tggctgtcca gacctggctc      180

agtgtgggcc tattcattgt cgcacacgac gctatgcacg ggtcgctggc gccgggtcgg      240

cctcgcctaa acgcagccgt aggcagactc accttagggc tctatgccgg tttccgattt      300

gatcgcctta aaacggccca ccacgcacat cacgccgccc ccggtactgc cgatgaccct      360

gatttctacg ccccagcacc gcgagcgttt ttgccttggt tcctcaactt ctttcgcact      420

tactttggct ggcgtgagat ggctgttctg acagctttag tcctaatagc cttgtttggt      480

ttgggtgccc ggccggcaaa tcttctcacg ttctgggcag ctccggcgct gttaagtgct      540

ctacagctat tcacttttgg cacgtggctg ccgcaccgcc acactgatca gccttttgcg      600

gacgcccacc atgctcggtc ttccggttac ggcccggtgc tcagcctgct tacctgtttt      660

cactttggtc ggcaccacga acaccatctg acaccgtggc gcccctggtg gcggctatgg      720


<210>  25
<211>  489
<212>  DNA
<213>  Athrospira plantensis

<400>  25
atgaaaaccc ccctaaccga agcagtttct atcgctgatt cccaaggtcg tttcctaagc       60

agcaccgaaa tccaagtagc ttttggccgt tttcgtcaag ccaaagctgg tctggaagct      120

gctaaagctt tgacctctaa agctgatagt ctgatcagtg gtgctgccca agcagtgtac      180

aacaagttcc cctacaccac ccaaatgcag ggacctaact acgcggcaga ccaacgcggt      240

aaggacaaat gtgctcgtga cataggctac tacctgcgga tggtaactta ttgcctgatt      300

gctggtggaa ctggccccat ggatgagtac ctgattgccg gtattgatga aatcaaccgg      360

actttcgagc tttctccaag ctggtacatt gaagccctga aatacatcaa agctaaccac      420

ggtttgtctg gtgacgctgc tgttgaagct aactcctacc tcgactacgc gatcaacgcc      480

ctgagctag                                                              489


<210>  26
<211>  519
<212>  DNA
<213>  Athroisma gracile

<400>  26
atgtttgatg ccttcaccaa ggtggtttct caagctgata ctcgcggcga aatgctgagt       60

acagctcaaa tcgatgctct gagccaaatg gttgctgaaa gcaacaaacg tttggattct      120

gttaaccgca ttaccagcaa cgcttccacc attgtttcca acgctgctcg ttctttgttc      180

gcagagcaac cccaactgat tgctcccggt ggaaacgcct acaccagccg tcgtatggct      240

gcttgcttgc gtgacatgga aatcatcctg cgctatgtaa cctacgctgt gtttgctggc      300

gatgcaagtg ttctcgaaga tcgttgcttg aacggtttgc gtgaaactta cctggctttg      360

ggaactcccg gttcttccgt tgctgtcggt gttggcaaaa tgaaagaagc tgctctggcg      420

atcgttaacg atcccgcagg tatcactcct ggtgattgta gcgctttggc ttcagaaatc      480

gctggttact ttgaccgtgc tgctgcagca gtttcctaa                             519


<210>  27
<211>  1406
<212>  DNA
<213>  Athrospira plantensis

<400>  27
ttgaaggggt gagaaggaag gcgaatgttg atttatgaag tttgattaac atttgtatca       60

aaatataaaa ttcttctcat aaaccctgta caatctttta agatttcgga aagtgttcta      120

ggatactgaa gaaatgaacc acggggcaat tgttaaaagc ctttgtcgat ggttcgcccc      180

ggaaggggtc ttaggaggtg acaccgatgg attgattgtc gtgatcattc atggtgtgtc      240

caatcccaac tcaactctaa gcaagtcaac aagtaggaga taaatccatg tttgatgcct      300

tcaccaaggt ggtttctcaa gctgatactc gcggcgaaat gctgagtaca gctcaaatcg      360

atgctctgag ccaaatggtt gctgaaagca acaaacgttt ggattctgtt aaccgcatta      420

ccagcaacgc ttccaccatt gtttccaacg ctgctcgttc tttgttcgca gagcaacccc      480

aactgattgc tcccggtgga aacgcctaca ccagccgtcg tatggctgct tgcttgcgtg      540

acatggaaat catcctgcgc tatgtaacct acgctgtgtt tgctggcgat gcaagtgttc      600

tcgaagatcg ttgcttgaac ggtttgcgtg aaacttacct ggctttggga actcccggtt      660

cttccgttgc tgtcggtgtt ggcaaaatga aagaagctgc tctggcgatc gttaacgatc      720

ccgcaggtat cactcctggt gattgtagcg ctttggcttc agaaatcgct ggttactttg      780

accgtgctgc tgcagcagtt tcctaatcaa gcagatccat agcatataac aattgaaaca      840

gtttagctga agtctaagtg actggacttc tgtttgttac ctaatttttt gtaaaccaat      900

cgggagataa ctcgagaatg aaaacccccc taaccgaagc agtttctatc gctgattccc      960

aaggtcgttt cctaagcagc accgaaatcc aagtagcttt tggccgtttt cgtcaagcca     1020

aagctggtct ggaagctgct aaagctttga cctctaaagc tgatagtctg atcagtggtg     1080

ctgcccaagc agtgtacaac aagttcccct acaccaccca aatgcaggga cctaactacg     1140

cggcagacca acgcggtaag gacaaatgtg ctcgtgacat aggctactac ctgcggatgg     1200

taacttattg cctgattgct ggtggaactg gccccatgga tgagtacctg attgccggta     1260

ttgatgaaat caaccggact ttcgagcttt ctccaagctg gtacattgaa gccctgaaat     1320

acatcaaagc taaccacggt ttgtctggtg acgctgctgt tgaagctaac tcctacctcg     1380

actacgcgat caacgccctg agctag                                          1406


