(19)
(11) EP 3 209 772 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
15.01.2025 Bulletin 2025/03

(45) Mention of the grant of the patent:
11.12.2024 Bulletin 2024/50

(21) Application number: 15853319.0

(22) Date of filing: 23.10.2015
(51) International Patent Classification (IPC): 
C12N 9/24(2006.01)
C12P 19/04(2006.01)
 C12P 19/18(2006.01)
C12N 9/10(2006.01)
(52) Cooperative Patent Classification (CPC):
C12P 19/18; C12N 9/24; C12Y 204/01065; C12N 9/2402; C12N 9/1051; C12Y 204/01
(86) International application number:
PCT/IB2015/058197
(87) International publication number:
WO 2016/063261 (28.04.2016 Gazette 2016/17)

(54)

MUTATED FUCOSIDASE

MUTIERTE FUCOSIDASE

FUCOSIDASE MUTÉE

(84) Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

(30) Priority: 24.10.2014 EP 14190374

(43) Date of publication of application:
30.08.2017 Bulletin 2017/35

(73) Proprietor: Glycom A/S
2970 Hørsholm (DK)

(72) Inventors:
  • CHAMPION, Elise
    31400 Toulouse (FR)
  • VOGEL, Andreas
    04105 Leipzig (DE)
  • BARTSCH, Sebastian
    04317 Leipzig (DE)
  • DEKANY, Gyula
    Sinnamon Park, Queensland 4073 (AU)

(74) Representative: Beck Greener LLP 
Fulwood House 12 Fulwood Place
London WC1V 6HR
London WC1V 6HR (GB)


(56) References cited: : 
EP-A1- 2 522 232
WO-A1-2012/156897
WO-A1-2013/190530
WO-A1-2013/190531
 WO-A1-2012/127410
WO-A1-2012/156898
WO-A1-2013/190531
WO-A2-2008/033520
   
  • OSANJO G. ET AL: "Directed Evolution of the alpha-L-Fucosidase from Thermotoga maritima into an alpha-L-Transfucosidase", BIOCHEMISTRY, vol. 46, no. 4, 1 January 2007 (2007-01-01), US, pages 1022 - 1033, XP055243536, ISSN: 0006-2960, DOI: 10.1021/bi061444w
  • SELA D. A. ET AL: "Bifidobacterium longum subsp. infantis ATCC 15697 alpha-Fucosidases Are Active on Fucosylated Human Milk Oligosaccharides", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 78, no. 3, 1 February 2012 (2012-02-01), pages 795 - 803, XP055274901, ISSN: 0099-2240, DOI: 10.1128/AEM.06762-11
  • D. TEZE ET AL: "Semi-rational approach for converting a GH1 -glycosidase into a -transglycosidase", PROTEIN ENGINEERING, DESIGN AND SELECTION, vol. 27, no. 1, 27 November 2013 (2013-11-27), GB, pages 13 - 19, XP055488740, ISSN: 1741-0126, DOI: 10.1093/protein/gzt057
  • OSANJO G ET AL.: "Directed evolution of the alpha-L- Fucosidase from Thermotoga maritima into an the alpha-L- Trandfucosidase", BIOCHEMISTRY, vol. 46, no. 4, 2007, pages 1022 - 1033, XP055243536
  • SELA D A ET AL.: "Bifidobacterium longum subsp. Infantis ATCC 15697 alpha-Fucosidases Are Active on Fucosylated Human Milk Oligosaccharides", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 78, no. 3, 2012, pages 795 - 803, XP055274901
  • OSANJO G O ET AL.: "Engineering the functional fitness of transglycosidases and glycosynthases by directed evolution", AFRICAN JOURNAL OF BIOTECHNOLOGY, vol. 10, no. 10, 2011, pages 1727 - 1735, XP055274903
   
Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).