(19)
(11) EP 3 209 781 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
15.01.2020 Bulletin 2020/03

(45) Mention of the grant of the patent:
04.12.2019 Bulletin 2019/49

(21) Application number: 15851895.1

(22) Date of filing: 20.10.2015
(51) International Patent Classification (IPC): 
C12N 15/54(2006.01)
(86) International application number:
PCT/US2015/056467
(87) International publication number:
WO 2016/064880 (28.04.2016 Gazette 2016/17)

(54)

MODIFIED TEMPLATE-INDEPENDENT ENZYMES FOR POLYDEOXYNUCLEOTIDE SYNTHESIS

MODIFIZIERTE VORLAGENUNABHÄNGIGE ENZYME ZUR POLYDEOXYNUKLEOTIDSYNTHESE

ENZYMES INDÉPENDANTES DE LA MATRICE MODIFIÉES POUR LA SYNTHÈSE DE POLYDÉSOXYNUCLÉOTIDES


(84) Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

(30) Priority: 20.10.2014 US 201462065976 P

(43) Date of publication of application:
30.08.2017 Bulletin 2017/35

(60) Divisional application:
19213236.3

(73) Proprietor: Molecular Assemblies Inc.
San Diego, CA 92121-1514 (US)

(72) Inventors:
  • EFCAVITCH, John William
    San Diego, CA 92124-2706 (US)
  • SYLVESTER, Juliesta E.
    San Diego, CA 92124-2706 (US)

(74) Representative: Kirkham, Nicholas Andrew et al
Graham Watt & Co. LLP St. Botolph's House 7-9 St. Botolph's Road
Sevenoaks, Kent TN13 3AJ
Sevenoaks, Kent TN13 3AJ (GB)


(56) References cited: : 
WO-A1-2016/028802
US-A1- 2004 043 396
US-A1- 2012 202 196
US-B1- 8 808 989
US-A1- 2004 043 396
US-A1- 2011 081 647
US-A1- 2014 141 414
   
  • DATABASE UniParc [Online] 31 January 2014 (2014-01-31), XP002777916, retrieved from UniProt Database accession no. UPI0003E329AC
  • Baoli Yangs ET AL: "Mutational Analysis of Residues in the Nucleotide Binding Domain of Human Terminal Deoxynucleotidyl Transferase", Journal of Biological Chemistry, 22 April 1994 (1994-04-22), pages 11859-11868, XP055345779, Retrieved from the Internet: URL:http://www.jbc.org/content/269/16/1185 9.full.pdf [retrieved on 2018-02-06]
  • YANG ET AL.: 'Mutational analysis of residues in the nucleotide binding domain of human terminal deoxynucleotidyl transferase' J BIOL CHEM. vol. 269, no. 16, 22 April 1994, pages 11859 8 - 11868, XP055345779
  • DELARUE ET AL.: 'Crystal structures of a template-independent DNA polymerase: murine terminal deoxynucleotidyltransferase' EMBO J. vol. 21, no. 3, 01 February 2002, pages 427 - 439, XP002767368 DOI: 10.1093/EMBOJ/21.3.427
   
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