(19)
(11) EP 3 305 889 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
23.06.2021 Bulletin 2021/25

(45) Mention of the grant of the patent:
12.05.2021 Bulletin 2021/19

(21) Application number: 16802959.3

(22) Date of filing: 26.04.2016
(51) International Patent Classification (IPC): 
C12N 5/00(2006.01)
C12N 1/00(2006.01)
(86) International application number:
PCT/JP2016/063006
(87) International publication number:
WO 2016/194522 (08.12.2016 Gazette 2016/49)

(54)

METHOD FOR INDUCING DIFFERENTIATION OF NEURAL CREST CELLS INTO NEURONS OF THE AUTONOMIC NERVOUS SYSTEM

VERFAHREN ZUR INDUZIERUNG DER DIFFERENZIERUNG VON ZELLEN DER NEURALLEISTE IN NERVENZELLEN DES AUTONOMEN NERVENSYSTEMS

PROCÉDÉ PERMETTANT D'INDUIRE UNE DIFFÉRENCIATION DES CELLULES DE CRÊTE NEURONALE EN NEURONES DU SYSTÈME NERVEUX AUTONOME


(84) Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

(30) Priority: 02.06.2015 JP 2015112237

(43) Date of publication of application:
11.04.2018 Bulletin 2018/15

(73) Proprietor: National Institute of Advanced Industrial Science and Technology
Tokyo 100-8921 (JP)

(72) Inventors:
  • KIDA, Yasuyuki
    Tsukuba-shi Ibaraki 305-8565 (JP)
  • TAKAYAMA, Yuzo
    Tsukuba-shi Ibaraki 305-8565 (JP)

(74) Representative: HGF 
1 City Walk
Leeds LS11 9DX
Leeds LS11 9DX (GB)


(56) References cited: : 
WO-A1-98/48001
   
  • R. E. MAINS: "PRIMARY CULTURES OF DISSOCIATED SYMPATHETIC NEURONS: I. Establishment of Long-Term Growth in Culture and Studies of Differentiated Properties", THE JOURNAL OF CELL BIOLOGY : JCB, vol. 59, no. 2, 1 November 1973 (1973-11-01), pages 329-345, XP55517347, US ISSN: 0021-9525, DOI: 10.1083/jcb.59.2.329
  • RACHAEL LUMB ET AL: "Sympathoadrenal neural crest cells: The known, unknown and forgotten?", DEVELOPMENT GROWTH AND DIFFERENTIATION., vol. 57, no. 2, 1 February 2015 (2015-02-01), pages 146-157, XP055517456, US ISSN: 0012-1592, DOI: 10.1111/dgd.12189
  • ELISABETH DUPIN ET AL: "Neural crest progenitors and stem cells: From early development to adulthood", DEVELOPMENTAL BIOLOGY, ELSEVIER, AMSTERDAM, NL, vol. 366, no. 1, 29 February 2012 (2012-02-29), pages 83-95, XP028485907, ISSN: 0012-1606, DOI: 10.1016/J.YDBIO.2012.02.035 [retrieved on 2012-03-08]
  • KOSUKE KIRINO ET AL: "Efficient derivation of sympathetic neurons from human pluripotent stem cells with a defined condition", SCIENTIFIC REPORTS, vol. 8, no. 1, 27 August 2018 (2018-08-27) , page 12865, XP055517714, DOI: 10.1038/s41598-018-31256-1
  • MIZUSEKI, KENJI ET AL.: 'Generation of neural crest-derived peripheral neurons and floor plate cells from mouse and primate embryonic stem cells' PNAS vol. 100, no. 10, 2003, pages 5828 - 5833, XP002484956
  • HARI, LISETTE ET AL.: 'Temporal control of neural crest lineage generation by Wnt/beta- katenin signaling' DEVELOPMENT vol. 139, 2012, pages 2107 - 2117, XP055332978
  • FUKUTA, MAKOTO ET AL.: 'Derivation of Mesenchymal Stromal Cells from Pluripotent Stem Cells through a Neural Crest Lineage using Small Molecule Compounds with Defined Media' PLOS ONE vol. 9, no. 12, 2014, page E112291, XP055332980
  • CHAMBERS, STUART M ET AL.: 'Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling' NATURE BIOTECHNOLOGY vol. 27, no. 3, 2009, pages 275 - 280, XP055007827
  • SUN, YILONG ET AL.: 'Ciliary neurotrophic factor(CNTF) has a dose-dependent biphasic effect on the number of adrenergic cells which develop in avian trunk neural crest cultures' NEUROSCIENCE LETTERS vol. 165, no. 1-2, 03 January 1994, pages 1 - 4, XP024164265 DOI: 10.1016/0304-3940(94)90695-5
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