SYSTEMS AND METHODS FOR SELECTION OF GRNA TARGETING STRANDS FOR CAS9 LOCALIZATION

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EP 3 344 766 B8

(12)	CORRECTED EUROPEAN PATENT SPECIFICATION
	Note: Bibliography reflects the latest situation

(15)	Correction information:
	Corrected version no 1 (W1 B1)

(48)	Corrigendum issued on:
	17.03.2021 Bulletin 2021/11

(45)	Mention of the grant of the patent:
	04.11.2020 Bulletin 2020/45

(21)	Application number: 16766178.4

(22)	Date of filing: 01.09.2016

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International Patent Classification (IPC):

C12N 15/10^(2006.01)
C12N 15/117^(2010.01)

C12Q 1/68^(2018.01)
C12N 15/85^(2006.01)

(86)	International application number:
	PCT/US2016/049921

(87)	International publication number:
	WO 2017/040793 (09.03.2017 Gazette 2017/10)

(54)	SYSTEMS AND METHODS FOR SELECTION OF GRNA TARGETING STRANDS FOR CAS9 LOCALIZATION SYSTEME UND VERFAHREN ZUR SELEKTION VON GRNS TARGETING STRÄNGEN ZUR CAS9 LOKALISATION SYSTÈMES ET PROCÉDÉS DE SÉLECTION DE BRINS CIBLANT L'ARNG POUR LA LOCALISATION DE CAS9

(84)	Designated Contracting States:
	AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

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Priority:

01.09.2015 US 201562212870 P

(43)	Date of publication of application:
	11.07.2018 Bulletin 2018/28

(73)	Proprietor: Dana-Farber Cancer Institute, Inc.
	Boston, MA 02215 (US)

(72)	Inventors:
	NOVINA, Carl Newton, Massachusetts 02459 (US) MEISTER, Glenna Boston, Massachusetts 02215 (US)

(74)	Representative: Haseltine Lake Kempner LLP
	138 Cheapside London EC2V 6BJ London EC2V 6BJ (GB)

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References cited: :

WO-A1-2015/021426
WO-A2-2016/103233

WO-A2-2015/089486

LUKE A. GILBERT ET AL: "CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes", CELL, vol. 154, no. 2, 1 July 2013 (2013-07-01), pages 442-451, XP055321615, US ISSN: 0092-8674, DOI: 10.1016/j.cell.2013.06.044
MEISTER G E ET AL: "An engineered split M.HhaI-zinc finger fusion lacks the intended methyltransferase specificity", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ELSEVIER, AMSTERDAM, NL, vol. 377, no. 1, 5 December 2008 (2008-12-05), pages 226-230, XP025587455, ISSN: 0006-291X, DOI: 10.1016/J.BBRC.2008.09.099 [retrieved on 2008-10-01]
LUKE A. GILBERT ET AL: "CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes", CELL, vol. 154, no. 2, 1 July 2013 (2013-07-01), pages 442-S5, XP055167546, ISSN: 0092-8674, DOI: 10.1016/j.cell.2013.06.044
LEI S. QI ET AL: "Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression", CELL, vol. 152, no. 5, 1 February 2013 (2013-02-01), pages 1173-1183, XP055299671, US ISSN: 0092-8674, DOI: 10.1016/j.cell.2013.02.022
HAN XU ET AL: "Sequence determinants of improved CRISPR sgRNA design", GENOME RESEARCH, vol. 25, no. 8, 1 August 2015 (2015-08-01) , pages 1147-1157, XP055321186, US ISSN: 1088-9051, DOI: 10.1101/gr.191452.115

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