(19)
(11) EP 3 350 334 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 2 (W2 B1)

(48) Corrigendum issued on:
26.02.2020 Bulletin 2020/09

(45) Mention of the grant of the patent:
13.11.2019 Bulletin 2019/46

(21) Application number: 16766970.4

(22) Date of filing: 19.09.2016
(51) International Patent Classification (IPC): 
C12N 15/866(2006.01)
C12N 15/85(2006.01)
B01L 99/00(2010.01)
 A01K 67/033(2006.01)
A61M 5/20(2006.01)
(86) International application number:
PCT/EP2016/072143
(87) International publication number:
WO 2017/046415 (23.03.2017 Gazette 2017/12)

(54)

EXPRESSION OF RECOMBINANT PROTEINS IN TRICHOPLUSIA NI PUPAE

EXPRESSION REKOMBINANTER PROTEINE IN TRICHOPLUSIA NI PUPAE

EXPRESSION DE PROTÉINES DE RECOMBINAISON DANS DES PUPES DE TRICHOPLUSIA NI

(84) Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

(30) Priority: 17.09.2015 EP 15382451

(43) Date of publication of application:
25.07.2018 Bulletin 2018/30

(60) Divisional application:
19206848.4

(73) Proprietor: Alternative Gene Expression, S.L.
28223 Pozuelo de Alarcón - Madrid (ES)

(72) Inventors:
  • MARTÍNEZ ESCRIBANO, José Ángel
    28223 Pozuelo de Alarcón - Madrid (ES)
  • ALVARADO FRADUA, Carmen
    28223 Pozuelo de Alarcón - Madrid (ES)
  • REYTOR SAAVEDRA, Edel
    28223 Pozuelo de Alarcón - Madrid (ES)
  • CID FERNANDEZ, Miguel
    28223 Pozuelo de Alarcón - Madrid (ES)

(74) Representative: Hoffmann Eitle 
Patent- und Rechtsanwälte PartmbB Arabellastraße 30
81925 München
81925 München (DE)


(56) References cited: : 
   
  • WÖLTJE MICHAEL ET AL: "Transgenic protein production in silkworm silk glands requires cathepsin and chitinase of Autographa californica multicapsid nucleopolyhedrovirus.", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY MAY 2014, vol. 98, no. 10, May 2014 (2014-05), pages 4571-4580, XP002756203, ISSN: 1432-0614
  • MOTOHASHI T ET AL: "Efficient large-scale protein production of larvae and pupae of silkworm by Bombyx mori nuclear polyhedrosis virus bacmid system", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 326, no. 3, 21 January 2005 (2005-01-21), pages 564-569, XP004679934, ISSN: 0006-291X, DOI: 10.1016/J.BBRC.2004.11.060
  • HELLERS M ET AL: "Expression of post-translational processing of preprocecropin A using a baculovirus vector.", EUROPEAN JOURNAL OF BIOCHEMISTRY / FEBS 15 JUL 1991, vol. 199, no. 2, 15 July 1991 (1991-07-15) , pages 435-439, XP002756204, ISSN: 0014-2956
  • DATABASE Taxonomy [Online] "Autographa californica nucleopolyhedrovirus", XP002756205, retrieved from NCBI Database accession no. 46015
  • DATABASE Taxonomy [Online] "Autographa californica nuclear polyhedrosis virus (AcMNPV)", XP002756206, retrieved from UniProt Database accession no. 46015
  • GÓMEZ-SEBASTIÁN SILVIA ET AL: "Significant productivity improvement of the baculovirus expression vector system by engineering a novel expression cassette.", PLOS ONE 2014, vol. 9, no. 5, E96562, 2014, pages 1-10, XP002756207, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0096562
  • JINN TZYY-RONG ET AL: "Aerosol infectivity of a Baculovirus to Trichoplusia ni larvae: An alternative larval inoculation strategy for recombinant protein production.", BIOTECHNOLOGY PROGRESS 2009 MAR-APR, vol. 25, no. 2, March 2009 (2009-03), pages 384-389, XP002756208, ISSN: 1520-6033
  • Anonymous: "Bac-to-Bac® TOPO® Expression System. An efficient cloning and site-specific transposition system to generate recombinant baculovirus for high-level protein expression Catalog nos. A11101, A11100", invitrogen - User Manuel , 15 December 2008 (2008-12-15), pages i-51, XP002756209, Retrieved from the Internet: URL:https://tools.thermofisher.com/content /sfs/manuals/bactobac_topo_exp_system_man. pdf [retrieved on 2016-04-05]
  • DATABASE WPI Week 201471 Thomson Scientific, London, GB; AN 2014-T03701 & CN 103 960 204 A (UNIV QINGDAO AGRIC) 6 August 2014 (2014-08-06)
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  • CHAZARRA ET AL: APPL BIOCHEM BIOTECHNOL, vol. 162, 2010, - 2010, pages 1834-1846,
  • "BAC-TO-BAC BACULOVIRUS EXPRESSION SYSTEM", 2015, INVITROGEN pages 1-80,
   
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