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(11)

EP 3 430 140 A1

(12)

(43) Date of publication: 23.01.2019 Bulletin 2019/04 (21) Application number: 17767655.8 (22) Date of filing: 17.03.2017

(51) International Patent Classification (IPC):  C12N 15/11(2006.01) C40B 20/00(2006.01) C12Q 1/68(2018.01) C40B 20/04(2006.01) (86) International application number: PCT/US2017/023019 (87) International publication number: WO 2017/161306 (21.09.2017 Gazette 2017/38)

(84) Designated Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR Designated Extension States: BA ME Designated Validation States: MA MD (30) Priority: 17.03.2016 US 201662309667 P (71) Applicant: Life Technologies Corporation Carlsbad, CA 92008 (US)	(72) Inventors: LI, Chieh-Yuan Hayward, California 94544 (US) JOUN, David Carlsbad, California 92008 (US) CHEN, Shiaw-Min Carlsbad, California 92008 (US) (74) Representative: Thermo Fisher Scientific- Life Sciences Solutions Group  Life Technologies Frankfurter Straße 129b 64293 Darmstadt 64293 Darmstadt (DE)