(19)
(11) EP 3 453 769 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
14.06.2023 Bulletin 2023/24

(45) Mention of the grant of the patent:
26.04.2023 Bulletin 2023/17

(21) Application number: 18194115.4

(22) Date of filing: 12.09.2018
(51) International Patent Classification (IPC): 
C12Q 1/6858(2018.01)
C12Q 1/686(2018.01)
(52) Cooperative Patent Classification (CPC):
C12Q 1/6858; C12Q 1/686
 
C-Sets:
  1. C12Q 1/6858, C12Q 2525/155, C12Q 2527/146, C12Q 2537/143, C12Q 2563/159, C12Q 2563/179, C12Q 2565/537;
  2. C12Q 1/686, C12Q 2525/155, C12Q 2527/146, C12Q 2537/143, C12Q 2563/159, C12Q 2563/179, C12Q 2565/537;


(54)

MULTIPLEX NUCLEIC ACID DETECTION METHOD

MULTIPLEX-NUKLEINSÄURENACHWEISVERFAHREN

PROCÉDÉ DE DÉTECTION D'ACIDES NUCLÉIQUES MULTIPLEX


(84) Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

(30) Priority: 12.09.2017 CN 201710815042

(43) Date of publication of application:
13.03.2019 Bulletin 2019/11

(73) Proprietor: LifeOS Genomics Corporation
KY1-1208, Cayman Islands (KY)

(72) Inventor:
  • LIU, Timothy Z
    Fremont, CA 94555 (US)

(74) Representative: Boult Wade Tennant LLP 
Salisbury Square House 8 Salisbury Square
London EC4Y 8AP
London EC4Y 8AP (GB)


(56) References cited: : 
WO-A1-2010/115100
US-A1- 2008 254 474
WO-A1-2012/129436
US-A1- 2014 378 320
   
  • CAO LEI ET AL: "Advances in digital polymerase chain reaction (dPCR) and its emerging biomedical applications", BIOSENSORS AND BIOELECTRONICS, ELSEVIER SCIENCE LTD. UK, AMSTERDAM, NL, vol. 90, 25 September 2016 (2016-09-25), pages 459-474, XP029862188, ISSN: 0956-5663, DOI: 10.1016/J.BIOS.2016.09.082
  • JASON E KREUTZ ET AL: "Theoretical design and analysis of multivolume digital assays with wide dynamic range validated experimentally with microfluidic digital PCR", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY, US , vol. 83, no. 21 1 November 2011 (2011-11-01), pages 8158-8168, XP002693075, ISSN: 0003-2700, DOI: 10.1021/JA2060116 Retrieved from the Internet: URL:http://pubs.acs.org/doi/abs/10.1021/ja 2060116
  • FENG SHEN ET AL: "Multiplexed Quantification of Nucleic Acids with Large Dynamic Range Using Multivolume Digital RT-PCR on a Rotational SlipChip Tested with HIV and Hepatitis C Viral Load", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 133, no. 44, 9 November 2011 (2011-11-09), pages 17705-17712, XP055054910, ISSN: 0002-7863, DOI: 10.1021/ja2060116
   
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