FERMENTATION TECHNIQUE TO IMPROVE PRODUCTION LEVEL OF RECOMBINANT HUMAN COLLAGEN

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EP 3 473 726 B8

(12)	CORRECTED EUROPEAN PATENT SPECIFICATION
	Note: Bibliography reflects the latest situation

(15)	Correction information:
	Corrected version no 1 (W1 B1)

(48)	Corrigendum issued on:
	16.10.2019 Bulletin 2019/42

(45)	Mention of the grant of the patent:
	11.09.2019 Bulletin 2019/37

(21)	Application number: 16909380.4

(22)	Date of filing: 18.10.2016

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International Patent Classification (IPC):

C12P 21/02^(2006.01)
C07K 14/78^(2006.01)

C12R 1/84^(2006.01)

(86)	International application number:
	PCT/CN2016/102435

(87)	International publication number:
	WO 2018/014453 (25.01.2018 Gazette 2018/04)

(54)	FERMENTATION TECHNIQUE TO IMPROVE PRODUCTION LEVEL OF RECOMBINANT HUMAN COLLAGEN GÄRUNGSVERFAHREN ZUR VERBESSERUNG DES HERSTELLUNGSVOLUMENS VON REKOMBINANTEM HUMANEM KOLLAGEN TECHNIQUE DE FERMENTATION POUR AMÉLIORER LE NIVEAU DE PRODUCTION DE COLLAGÈNE HUMAIN RECOMBINANT

(84)	Designated Contracting States:
	AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

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Priority:

22.07.2016 CN 201610587403

(43)	Date of publication of application:
	24.04.2019 Bulletin 2019/17

(73)	Proprietor: Jiangsu Jland Biotech Co., Ltd.
	Jingjiang, Jiangsu (CN)

(72)	Inventors:
	YANG, Shulin Nanjing Jiangsu 210094 (CN) DU, Erfeng Jingjiang Jiangsu 214500 (CN) HUANG, Jianmin Jingjiang Jiangsu 214500 (CN) GAO, Lihu Jingjiang Jiangsu 214500 (CN) ZHAO, Jianfeng Jingjiang Jiangsu 214500 (CN) TAO, Hai Jingjiang Jiangsu 214500 (CN) FENG, Liping Jingjiang Jiangsu 214500 (CN) ZHOU, Aimei Jingjiang Jiangsu 214500 (CN)

(74)	Representative: Sun, Yiming
	HUASUN Patent- und Rechtsanwälte Friedrichstraße 33 80801 München 80801 München (DE)

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References cited: :

WO-A2-2014/170460
CN-A- 104 561 201

CN-A- 103 725 623

G. CORNELISSEN ET AL: "Production of Recombinant Proteins withPichia pastoris in Integrated Processing", ENGINEERING IN LIFE SCIENCES, vol. 3, no. 9, 10 September 2003 (2003-09-10), pages 361-370, XP55566097, DE ISSN: 1618-0240, DOI: 10.1002/elsc.200300033
EDA Ã?ELIK ET AL: "Metabolic flux analysis for recombinant protein production by Pichia pastoris using dual carbon sources: Effects of methanol feeding rate", BIOTECHNOLOGY AND BIOENGINEERING, vol. 105, no. 2, 1 February 2010 (2010-02-01), pages 317-329, XP55566103, ISSN: 0006-3592, DOI: 10.1002/bit.22543
H.T Ren ET AL: "Modeling and Model Based Feeding Control for Pichia pastoris fed-batch cultivation", Advanced Control of Chemical Processes, 1 January 2004 (2004-01-01), XP055566790, Retrieved from the Internet: URL:https://ac.els-cdn.com/S14746670173877 36/1-s2.0-S1474667017387736-main.pdf?_tid= a8b997d4-5c4f-4916-b991-994f1e6f7e2f&acdna t=1552291044_0f55530f3ad5cd812d2403acd8770 5af [retrieved on 2019-03-11]
H. YIN et al.: "Intracellular Expression and Purification of the Canstatin-N Protein in Pichia Pastoris", Gene, vol. 504, no. 1, 2 May 2015 (2015-05-02), pages 122-126, XP055452700,
LI, BAOKU et al.: "Study on the Culture Conditions of L-lactic Acid Bacteria", Liquor-Making Science & Technology, 31 December 2007 (2007-12-31), pages 51-57, XP009511442,

Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).