(19)
(11) EP 3 480 321 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
10.03.2021 Bulletin 2021/10

(45) Mention of the grant of the patent:
09.12.2020 Bulletin 2020/50

(21) Application number: 18195513.9

(22) Date of filing: 28.08.2014
(51) International Patent Classification (IPC): 
C12Q 1/68(2018.01)

(54)

MASSIVELY PARALLEL SINGLE CELL ANALYSIS

MASSIV PARALLELE EINZELZELLANALYSE

ANALYSE DE CELLULE UNIQUE MASSIVEMENT PARALLÈLE


(84) Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

(30) Priority: 28.08.2013 US 201361871232 P
12.03.2014 US 201461952036 P
13.06.2014 US 201462012237 P

(43) Date of publication of application:
08.05.2019 Bulletin 2019/19

(60) Divisional application:
20209777.0

(62) Application number of the earlier application in accordance with Art. 76 EPC:
14761937.3 / 3039158

(73) Proprietor: Becton, Dickinson and Company
Franklin Lakes, NJ 07417 (US)

(72) Inventors:
  • FAN, Christina
    Fremont, CA 94538 (US)
  • FODOR, Stephen, P.a.
    Palo Alto, CA 94301 (US)
  • FU, Glenn, K.
    Dublin, CA 94568 (US)
  • FACER, Geoffrey, Richard
    Redwood City, CA 94061 (US)
  • WILHELMY, Julie
    Santa Cruz, CA 95060 (US)

(74) Representative: Chapman, Desmond Mark 
Carpmaels & Ransford LLP One Southampton Row
London WC1B 5HA
London WC1B 5HA (GB)


(56) References cited: : 
WO-A1-2012/048341
WO-A2-2012/083225
WO-A2-2010/117620
US-A1- 2013 116 130
   
  • HUBERT HUG ET AL: "Measurement of the Number of Molecules of a Single mRNA Species in a Complex mRNA Preparation", JOURNAL OF THEORETICAL BIOLOGY., vol. 221, no. 4, 1 April 2003 (2003-04-01) , pages 615-624, XP055569630, GB ISSN: 0022-5193, DOI: 10.1006/jtbi.2003.3211
  • G. K. FU ET AL: "Counting individual DNA molecules by the stochastic attachment of diverse labels", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 108, no. 22, 31 May 2011 (2011-05-31) , pages 9026-9031, XP055164127, ISSN: 0027-8424, DOI: 10.1073/pnas.1017621108
  • C. B. JABARA ET AL: "Accurate sampling and deep sequencing of the HIV-1 protease gene using a Primer ID", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 108, no. 50, 30 November 2011 (2011-11-30), pages 20166-20171, XP055161674, ISSN: 0027-8424, DOI: 10.1073/pnas.1110064108
  • I. KINDE ET AL: "Detection and quantification of rare mutations with massively parallel sequencing", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 108, no. 23, 17 May 2011 (2011-05-17) , pages 9530-9535, XP055164202, ISSN: 0027-8424, DOI: 10.1073/pnas.1105422108
  • G. MIKE MAKRIGIORGOS ET AL: "A PCR-based amplification method retaining the quantitative difference between two complex genomes", NATURE BIOTECHNOLOGY, vol. 20, no. 9, 1 September 2002 (2002-09-01), pages 936-939, XP055023044, ISSN: 1087-0156, DOI: 10.1038/nbt724
   
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