(19)
(11) EP 3 494 214 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
04.09.2024 Bulletin 2024/36

(45) Mention of the grant of the patent:
24.07.2024 Bulletin 2024/30

(21) Application number: 17837697.6

(22) Date of filing: 03.08.2017
(51) International Patent Classification (IPC): 
C12N 9/00(2006.01)
C12N 9/12(2006.01)
C12P 19/34(2006.01)
 C12N 9/10(2006.01)
C12N 15/10(2006.01)
C12Q 1/6844(2018.01)
(52) Cooperative Patent Classification (CPC):
C12N 15/1065; C12N 15/1096; C12Q 1/6844; C12Q 1/6806
 
C-Sets:
C12Q 1/6844, C12Q 2521/107, C12Q 2521/327, C12Q 2521/507, C12Q 2525/191, C12Q 2563/159, C12Q 2563/179, C12Q 2565/514;
(86) International application number:
PCT/US2017/045324
(87) International publication number:
WO 2018/027048 (08.02.2018 Gazette 2018/06)

(54)

METHOD FOR PREPARING CDNA LIBRARIES

VERFAHREN ZUR HERSTELLUNG VON CDNA BIBLIOTHEKEN

PROCÉDURE DE FORMATION DES BANQUES D'ADNC

(84) Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

(30) Priority: 05.08.2016 US 201662371638 P
20.06.2017 US 201762522232 P

(43) Date of publication of application:
12.06.2019 Bulletin 2019/24

(60) Divisional application:
24185978.4

(73) Proprietors:
  • Bio-Rad Laboratories, Inc.
    Hercules, CA 94547 (US)
  • Bio-Rad Europe GmbH
    4051 Basel (CH)

(72) Inventors:
  • LEBOFSKY, Ronald
    4051 Basel (CH)
  • AGRESTI, Jeremy
    Hercules, California 94547-1803 (US)

(74) Representative: dompatent von Kreisler Selting Werner - Partnerschaft von Patent- und Rechtsanwälten mbB 
Deichmannhaus am Dom Bahnhofsvorplatz 1
50667 Köln
50667 Köln (DE)


(56) References cited: : 
WO-A1-2012/040387
WO-A1-2014/201273
WO-A2-2008/074891
WO-A2-2012/162267
US-A1- 2006 281 092
US-A1- 2015 291 942
US-A1- 2016 053 253
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WO-A1-2015/029031
WO-A2-2010/053587
WO-A2-2013/163263
US-A1- 2010 120 098
US-A1- 2015 329 891
US-B2- 9 074 204
   
  • J. GERTZ ET AL: "Transposase mediated construction of RNA-seq libraries", GENOME RESEARCH, vol. 22, no. 1, 1 January 2012 (2012-01-01), pages 134 - 141, XP055050772, ISSN: 1088-9051, DOI: 10.1101/gr.127373.111
  • SAIFUL ISLAM ET AL: "Characterization of the single-cell transcriptional landscape by highly multiplex RNA-seq", vol. 21, no. 7, 1 July 2011 (2011-07-01), pages 1160 - 1167, XP002682367, ISSN: 1088-9051, Retrieved from the Internet <URL:http://genome.cshlp.org/content/21/7/1160> [retrieved on 20110504], DOI: 10.1101/GR.110882.110
  • NICK CARUCCIO ET AL: "Nextera(TM) Technology for NGS DNA Library Preparation: Simultaneous Fragmentation and Tagging by In Vitro Transposition", 1 October 2009 (2009-10-01), XP055303813, Retrieved from the Internet <URL:http://www.epibio.com/docs/default-source/forum-archive/forum-16-3---nextera-technology-for-ngs-dna-library-preparation---simultaneous-fragmentation-and-tagging-by-in-vitro-transposition.pdf?sfvrsn=4> [retrieved on 20160919]
 
Remarks:
The file contains technical information submitted after the application was filed and not included in this specification
 
Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).