THREE-DIMENSIONAL IN VITRO ALVEOLAR LUNG MODEL, PROCESS FOR PREPARING SAID MODEL, AND ITS USE FOR DETERMINING AND /OR PREDICTING THE SENSITIZING EFFECTS OF INHALABLE PRODUCTS

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EP 3 562 935 B8

(12)	CORRECTED EUROPEAN PATENT SPECIFICATION
	Note: Bibliography reflects the latest situation

(15)	Correction information:
	Corrected version no 1 (W1 B1)

(48)	Corrigendum issued on:
	29.12.2021 Bulletin 2021/52

(45)	Mention of the grant of the patent:
	06.10.2021 Bulletin 2021/40

(21)	Application number: 17828760.3

(22)	Date of filing: 26.12.2017

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International Patent Classification (IPC):

C12N 5/071^(2010.01)
G01N 33/50^(2006.01)

C12N 5/0784^(2010.01)

(52)	Cooperative Patent Classification (CPC):
	C12N 2502/27; G01N 33/5088; C12N 2502/1114; C12N 2502/1121; C12N 2502/1157; C12N 2503/04; C12N 2502/28; C12N 5/0697; C12N 5/0639; C12N 5/0688

(86)	International application number:
	PCT/EP2017/084583

(87)	International publication number:
	WO 2018/122219 (05.07.2018 Gazette 2018/27)

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THREE-DIMENSIONAL IN VITRO ALVEOLAR LUNG MODEL, PROCESS FOR PREPARING SAID MODEL, AND ITS USE FOR DETERMINING AND /OR PREDICTING THE SENSITIZING EFFECTS OF INHALABLE PRODUCTS

DREIDIMENSIONALES IN VITRO ALVEOLAR-LUNGENMODELL, VERFAHREN ZUR HERSTELLUNG DIESES MODELLS UND SEINE VERWENDUNG ZUR BESTIMMUNG UND/ODER VORHERSAGE DER SENSIBILISIERENDEN WIRKUNG VON INHALIERBAREN PRODUKTEN

MODÈLE TRIDIMENSIONNEL IN VITRO DE POUMON ALVÉOLAIRE, PROCÉDÉ DE PRÉPARATION DUDIT MODÈLE, ET SON UTILISATION POUR DÉTERMINER ET/OU PRÉDIRE LES EFFETS SENSIBILISANTS DE PRODUITS INHALABLES

(84)	Designated Contracting States:
	AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

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Priority:

27.12.2016 LU 93401

(43)	Date of publication of application:
	06.11.2019 Bulletin 2019/45

(73)	Proprietor: Luxembourg Institute of Science and Technology (LIST)
	4362 Esch-sur-Alzette (LU)

(72)	Inventors:
	CHARY, Aline 57390 Audun-le-Tiche (FR) GUTLEB, Arno 5552 Remich (LU) SERCHI, Tommaso 57480 Kirsch-les-Sierck (FR) BLÖMEKE, Brunehilde 54136 Pluwig (DE)

(74)	Representative: Ipsilon
	Le Centralis 63, avenue du Général Leclerc 92340 Bourg-la-Reine 92340 Bourg-la-Reine (FR)

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References cited: :

WO-A1-2009/048661

SEBASTIAN G KLEIN ET AL: "An improved 3D tetraculture system mimicking the cellular organisation at the alveolar barrier to study the potential toxic effects of particles on the lung", PARTICLE AND FIBRE TOXICOLOGY, vol. 10, no. 1, 1 January 2013 (2013-01-01), page 31, XP055340125, GB ISSN: 1743-8977, DOI: 10.1186/1743-8977-10-31
L. MULLER ET AL: "Oxidative stress and inflammation response after nanoparticle exposure: differences between human lung cell monocultures and an advanced three-dimensional model of the human epithelial airways", JOURNAL OF THE ROYAL SOCIETY. INTERFACE, vol. 7, no. Suppl_1, 8 July 2009 (2009-07-08), pages S27-S40, XP055340104, GB ISSN: 1742-5689, DOI: 10.1098/rsif.2009.0161.focus
CARSTEN SCHLEH ET AL: "Surfactant Protein D modulates allergen particle uptake and inflammatory response in a human epithelial airway model", RESPIRATORY RESEARCH, BIOMED CENTRAL LTD., LONDON, GB, vol. 13, no. 1, 1 February 2012 (2012-02-01), page 8, XP021117669, ISSN: 1465-9921, DOI: 10.1186/1465-9921-13-8
CRISTIANE DE SOUZA CARVALHO ET AL: "Carrier interactions with the biological barriers of the lung: Advanced in vitro models and challenges for pulmonary drug delivery", ADVANCED DRUG DELIVERY REVIEWS, vol. 75, 1 August 2014 (2014-08-01), pages 129-140, XP055340131, AMSTERDAM, NL ISSN: 0169-409X, DOI: 10.1016/j.addr.2014.05.014
E. ALFARO-MORENO ET AL: "Co-cultures of multiple cell types mimic pulmonary cell communication in response to urban PM10", EUROPEAN RESPIRATORY JOURNAL., vol. 32, no. 5, 1 November 2008 (2008-11-01), pages 1184-1194, XP055340159, DK ISSN: 0903-1936, DOI: 10.1183/09031936.00044008

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