               SEQUENCE LISTING

<110> INNOVATIVE TECHNOLOGIES IN BIOLOGICAL SYSTEMS SL

<120> Fluorescent fusion polypeptide capable of detecting phosphorylation of cellular membrane receptors by GRKs (G-protein receptor kinases) and/or Receptor tyrosine kinases (RTKs).

<130> HE- Ref: 902 914

<140> EP17382049.9
<141> 2017-02-06

<160> 4

<170> BiSSAP 1.3.6

<210> 1
<211> 1701
<212> DNA
<213> Artificial Sequence


<220> 
<223> phosphorylated receptor binding peptide from b-arrestin

<400> 1
atggacagct acctgctgat gtggggcctg ctgaccttca tcatggtgcc cggctgccag      60

gccgagctgt gcgacgacga cccccctgag atcccccacg ccaccttcaa agccatggcc     120

tacaaagaag gcaccatgct gaactgcgag tgcaagcggg gcttccggcg gatcaagagc     180

ggcagcctgt acatgctgtg caccggcaac agcagccaca gcagctggga caaccagtgc     240

cagtgcacca gcagcgccac ccggaacacc accaaacagg tcacacccca gcccgaggaa     300

cagaaagagc gcaagaccac cgagatgcag agccccatgc agcccgtgga ccaggcctct     360

ctgcccggcc actgcagaga gcccccacct tgggagaacg aggccaccga gcggatctac     420

cacttcgtgg tcggacagat ggtgtactac cagtgcgtgc agggctaccg ggccctgcac     480

agaggacctg ccgagagcgt gtgcaagatg acccacggca agacccggtg gacccagccc     540

cagctgatct gcaccggcga gatggaaacc agccagttcc ccggcgagga aaagccccag     600

gccagccctg agggcagacc cgagagcgag acaagctgcc tggtgacaac caccgacttc     660

cagatccaga ccgagatggc cgccacaatg gaaacctcca tcttcaccac cgacctgcag     720

gtggccgtgg ccggctgcgt gttcctgctg atctctgtgc tgctcctgag cggcctgacc     780

tggcagcgga gacagagaaa gagcggccgg accatcggga tccaactagt tgtcgaccag     840

cagcagcagc agcagggaat tctgcagtcg acggtaccaa tggtgggtga ggatagcgag     900

ctgatcaccg agaacatgca catgaaactg tacatggagg gcaccgtgaa caaccaccac     960

ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac catgaagatc    1020

aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg    1080

tacggcagca aagccttcat caaccacacc cagggcatcc ccgacttctt taagcagtcc    1140

ttccctgagg gcttcacatg ggagagaatc accacatacg aagacggggg cgtgctgacc    1200

gctacccagg acaccagcct ccagaacggc tgcctcatct acaacgtcaa gatcaacggg    1260

gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccagc    1320

accgagatgc tgtaccccgc tgacagcggc ctgagaggcc atggccagat ggccctgaag    1380

ctcgtgggcg ggggctacct gcactgctcc ctcaagacca catacagatc caagaaaccc    1440

gctaagaacc tcaagatgcc cggcttccac ttcgtggacc acagactgga aagaatcaag    1500

gaggccgaca aagagaccta cgtcgagcag cacgagatgg ctgtggccaa gtactgcgac    1560

ctccctagca aactggggca cagcagatct cgagtaggcg gcggcggcta tggccgtgaa    1620

gacctggatg tgctgggctt gtccttccgc aaagacctgg gcggcctcat tgaatttggc    1680

ggcggccggc ttcggctgaa g                                              1701


<210> 2
<211> 567
<212> PRT
<213> Artificial Sequence


<220> 
<223> phosphorylated receptor binding peptide from b-arrestin

<400> 2
Met Asp Ser Tyr Leu Leu Met Trp Gly Leu Leu Thr Phe Ile Met Val 
1               5                   10                  15      
Pro Gly Cys Gln Ala Glu Leu Cys Asp Asp Asp Pro Pro Glu Ile Pro 
            20                  25                  30          
His Ala Thr Phe Lys Ala Met Ala Tyr Lys Glu Gly Thr Met Leu Asn 
        35                  40                  45              
Cys Glu Cys Lys Arg Gly Phe Arg Arg Ile Lys Ser Gly Ser Leu Tyr 
    50                  55                  60                  
Met Leu Cys Thr Gly Asn Ser Ser His Ser Ser Trp Asp Asn Gln Cys 
65                  70                  75                  80  
Gln Cys Thr Ser Ser Ala Thr Arg Asn Thr Thr Lys Gln Val Thr Pro 
                85                  90                  95      
Gln Pro Glu Glu Gln Lys Glu Arg Lys Thr Thr Glu Met Gln Ser Pro 
            100                 105                 110         
Met Gln Pro Val Asp Gln Ala Ser Leu Pro Gly His Cys Arg Glu Pro 
        115                 120                 125             
Pro Pro Trp Glu Asn Glu Ala Thr Glu Arg Ile Tyr His Phe Val Val 
    130                 135                 140                 
Gly Gln Met Val Tyr Tyr Gln Cys Val Gln Gly Tyr Arg Ala Leu His 
145                 150                 155                 160 
Arg Gly Pro Ala Glu Ser Val Cys Lys Met Thr His Gly Lys Thr Arg 
                165                 170                 175     
Trp Thr Gln Pro Gln Leu Ile Cys Thr Gly Glu Met Glu Thr Ser Gln 
            180                 185                 190         
Phe Pro Gly Glu Glu Lys Pro Gln Ala Ser Pro Glu Gly Arg Pro Glu 
        195                 200                 205             
Ser Glu Thr Ser Cys Leu Val Thr Thr Thr Asp Phe Gln Ile Gln Thr 
    210                 215                 220                 
Glu Met Ala Ala Thr Met Glu Thr Ser Ile Phe Thr Thr Asp Leu Gln 
225                 230                 235                 240 
Val Ala Val Ala Gly Cys Val Phe Leu Leu Ile Ser Val Leu Leu Leu 
                245                 250                 255     
Ser Gly Leu Thr Trp Gln Arg Arg Gln Arg Lys Ser Gly Arg Thr Ile 
            260                 265                 270         
Gly Ile Gln Leu Val Val Asp Gln Gln Gln Gln Gln Gln Gly Ile Leu 
        275                 280                 285             
Gln Ser Thr Val Pro Met Val Gly Glu Asp Ser Glu Leu Ile Thr Glu 
    290                 295                 300                 
Asn Met His Met Lys Leu Tyr Met Glu Gly Thr Val Asn Asn His His 
305                 310                 315                 320 
Phe Lys Cys Thr Ser Glu Gly Glu Gly Lys Pro Tyr Glu Gly Thr Gln 
                325                 330                 335     
Thr Met Lys Ile Lys Val Val Glu Gly Gly Pro Leu Pro Phe Ala Phe 
            340                 345                 350         
Asp Ile Leu Ala Thr Ser Phe Met Tyr Gly Ser Lys Ala Phe Ile Asn 
        355                 360                 365             
His Thr Gln Gly Ile Pro Asp Phe Phe Lys Gln Ser Phe Pro Glu Gly 
    370                 375                 380                 
Phe Thr Trp Glu Arg Ile Thr Thr Tyr Glu Asp Gly Gly Val Leu Thr 
385                 390                 395                 400 
Ala Thr Gln Asp Thr Ser Leu Gln Asn Gly Cys Leu Ile Tyr Asn Val 
                405                 410                 415     
Lys Ile Asn Gly Val Asn Phe Pro Ser Asn Gly Pro Val Met Gln Lys 
            420                 425                 430         
Lys Thr Leu Gly Trp Glu Ala Ser Thr Glu Met Leu Tyr Pro Ala Asp 
        435                 440                 445             
Ser Gly Leu Arg Gly His Gly Gln Met Ala Leu Lys Leu Val Gly Gly 
    450                 455                 460                 
Gly Tyr Leu His Cys Ser Leu Lys Thr Thr Tyr Arg Ser Lys Lys Pro 
465                 470                 475                 480 
Ala Lys Asn Leu Lys Met Pro Gly Phe His Phe Val Asp His Arg Leu 
                485                 490                 495     
Glu Arg Ile Lys Glu Ala Asp Lys Glu Thr Tyr Val Glu Gln His Glu 
            500                 505                 510         
Met Ala Val Ala Lys Tyr Cys Asp Leu Pro Ser Lys Leu Gly His Ser 
        515                 520                 525             
Arg Ser Arg Val Gly Gly Gly Gly Tyr Gly Arg Glu Asp Leu Asp Val 
    530                 535                 540                 
Leu Gly Leu Ser Phe Arg Lys Asp Leu Gly Gly Leu Ile Glu Phe Gly 
545                 550                 555                 560 
Gly Gly Arg Leu Arg Leu Lys 
                565         

<210> 3
<211> 272
<212> PRT
<213> Artificial Sequence


<220> 
<223> extracellular domain of interleukin-2 receptor

<400> 3
Met Asp Ser Tyr Leu Leu Met Trp Gly Leu Leu Thr Phe Ile Met Val 
1               5                   10                  15      
Pro Gly Cys Gln Ala Glu Leu Cys Asp Asp Asp Pro Pro Glu Ile Pro 
            20                  25                  30          
His Ala Thr Phe Lys Ala Met Ala Tyr Lys Glu Gly Thr Met Leu Asn 
        35                  40                  45              
Cys Glu Cys Lys Arg Gly Phe Arg Arg Ile Lys Ser Gly Ser Leu Tyr 
    50                  55                  60                  
Met Leu Cys Thr Gly Asn Ser Ser His Ser Ser Trp Asp Asn Gln Cys 
65                  70                  75                  80  
Gln Cys Thr Ser Ser Ala Thr Arg Asn Thr Thr Lys Gln Val Thr Pro 
                85                  90                  95      
Gln Pro Glu Glu Gln Lys Glu Arg Lys Thr Thr Glu Met Gln Ser Pro 
            100                 105                 110         
Met Gln Pro Val Asp Gln Ala Ser Leu Pro Gly His Cys Arg Glu Pro 
        115                 120                 125             
Pro Pro Trp Glu Asn Glu Ala Thr Glu Arg Ile Tyr His Phe Val Val 
    130                 135                 140                 
Gly Gln Met Val Tyr Tyr Gln Cys Val Gln Gly Tyr Arg Ala Leu His 
145                 150                 155                 160 
Arg Gly Pro Ala Glu Ser Val Cys Lys Met Thr His Gly Lys Thr Arg 
                165                 170                 175     
Trp Thr Gln Pro Gln Leu Ile Cys Thr Gly Glu Met Glu Thr Ser Gln 
            180                 185                 190         
Phe Pro Gly Glu Glu Lys Pro Gln Ala Ser Pro Glu Gly Arg Pro Glu 
        195                 200                 205             
Ser Glu Thr Ser Cys Leu Val Thr Thr Thr Asp Phe Gln Ile Gln Thr 
    210                 215                 220                 
Glu Met Ala Ala Thr Met Glu Thr Ser Ile Phe Thr Thr Asp Leu Gln 
225                 230                 235                 240 
Val Ala Val Ala Gly Cys Val Phe Leu Leu Ile Ser Val Leu Leu Leu 
                245                 250                 255     
Ser Gly Leu Thr Trp Gln Arg Arg Gln Arg Lys Ser Gly Arg Thr Ile 
            260                 265                 270         



<210> 4
<211> 31
<212> PRT
<213> Artificial Sequence


<220> 
<223> Chlatrin and beta-adaptin peptide from arrestin

<400> 4
Tyr Gly Arg Glu Asp Leu Asp Val Leu Gly Leu Ser Phe Arg Lys Asp 
1               5                   10                  15      
Leu Gly Gly Leu Ile Glu Phe Gly Gly Gly Arg Leu Arg Leu Lys 
            20                  25                  30      

