(19)
(11) EP 3 668 971 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
29.05.2024 Bulletin 2024/22

(45) Mention of the grant of the patent:
10.04.2024 Bulletin 2024/15

(21) Application number: 18845635.4

(22) Date of filing: 17.08.2018
(51) International Patent Classification (IPC): 
C12N 9/12(2006.01)
C12N 15/10(2006.01)
(52) Cooperative Patent Classification (CPC):
C12N 9/1247
(86) International application number:
PCT/US2018/046989
(87) International publication number:
WO 2019/036682 (21.02.2019 Gazette 2019/08)

(54)

RNA POLYMERASE VARIANTS

RNA-POLYMERASEVARIANTEN

VARIANTS D'ARN POLYMÉRASE


(84) Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated Extension States:
BA ME
Designated Validation States:
KH MA MD TN

(30) Priority: 18.08.2017 US 201762547677 P
09.02.2018 US 201862628484 P
05.03.2018 US 201862638684 P
29.05.2018 US 201862677527 P

(43) Date of publication of application:
24.06.2020 Bulletin 2020/26

(60) Divisional application:
24162789.2

(73) Proprietor: ModernaTX, Inc.
Cambridge, MA 02142 (US)

(72) Inventors:
  • RABIDEAU, Amy, E.
    Waltham, MA 02451 (US)
  • DOUSIS, Athanasios
    Cambridge, MA 02139 (US)
  • RAVICHANDRAN, Kanchana
    Cambridge, MA 02139 (US)
  • HOBERT, Elissa
    Cambridge, MA 02140 (US)

(74) Representative: Mathys & Squire 
The Shard 32 London Bridge Street
London SE1 9SG
London SE1 9SG (GB)


(56) References cited: : 
WO-A1-2013/102203
WO-A1-2017/066789
WO-A1-2017/123748
JP-A- 2011 223 982
US-B1- 10 034 951
WO-A1-2017/053297
WO-A1-2017/070624
WO-A1-2018/053209
US-A1- 2011 143 397
   
  • TANG G-Q et al.: "Relaxed Rotational and Scrunching Changes in P266L Mutant of T7 RNA Polymerase Reduce Short Abortive RNAs while Delaying Transition into Elongation", PLOS One, vol. 9 20 March 2014 (2014-03-20), XP055571018, Retrieved from the Internet: URL:https://doi.org/10.1371/journal.pone.0 091859
  • BANDWAR RP et al.: "The Transition to an Elongation Complex by T7 RNA Polymerase Is a Multistep Process", The Journal of Biological Chemistry, vol. 282, 2007, pages 22879-22886, XP055571029,
  • GARDNER LP et al.: "Initiation, Elongation, and Processivity of Carboyxl-Terminal Mutants of T7 RNA Polymerase", Biochemistry, vol. 36, 1997, pages 2908-2918, XP002676622,
  • MA K et al.: "Probing conformational changes in T7 RNA polymerase during initation and termination by using engineered disulphide linkages", PNAS, vol. 102, 2005, pages 17612-17617, XP055005956,
  • BANDWAR, RP et al.: "Sequential Release of Promoter Contacts during Transcription Initiation to Elongation Transition", J Mol Biol, vol. 360, 2006, pages 466-482, XP024951191,
  • BRIEBA LG et al.: "Scanning Mutagenesis Reveals Roles for Helix N of the Bacteriophage T7 RNA Polymerase Thumb Subdomain in Transcription Complex Stability, Pausing, and Termination", The Journal of Biological Chemistry, vol. 276, 21 December 2000 (2000-12-21), pages 10306-10313, XP055571050,
  • GAAL T et al.: "DNA-binding determinants of the alpha subunit of RNA polymerase: novel DNA-binding domain architecture", Genes & Development, vol. 10, 1996, pages 16-26, XP055571056,
   
Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).