(19)
(11) EP 3 684 381 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
07.12.2022 Bulletin 2022/49

(45) Mention of the grant of the patent:
02.11.2022 Bulletin 2022/44

(21) Application number: 18858922.0

(22) Date of filing: 20.09.2018
(51) International Patent Classification (IPC): 
C12P 1/00(2006.01)
(52) Cooperative Patent Classification (CPC):
C12P 1/00
(86) International application number:
PCT/US2018/052074
(87) International publication number:
WO 2019/060629 (28.03.2019 Gazette 2019/13)

(54)

PRODUCTION OF EXTRACELLULAR VESICLES IN SINGLE-CELL SUSPENSION USING CHEMICALLY-DEFINED CELL CULTURE MEDIA

HERSTELLUNG VON EXTRAZELLULÄREN VESIKELN IN EINZELZELLSUSPENSION UNTER VERWENDUNG VON CHEMISCH DEFINIERTEN ZELLKULTURMEDIEN

PRODUCTION DE VÉSICULES EXTRACELLULAIRES DANS UNE SUSPENSION DE CELLULES ISOLÉES À L'AIDE DE MILIEUX DE CULTURE CELLULAIRE CHIMIQUEMENT DÉFINIS

(84) Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

(30) Priority: 21.09.2017 US 201762561206 P
07.05.2018 US 201862668217 P

(43) Date of publication of application:
29.07.2020 Bulletin 2020/31

(60) Divisional application:
22204880.3

(73) Proprietor: Codiak BioSciences, Inc.
Cambridge, MA 02140 (US)

(72) Inventors:
  • VILLIGER, Agata A.
    Cambridge, MA 02139 (US)
  • GRUBE, Andrew F.
    Cambridge, MA 02139 (US)
  • CHAN, Tik Yan
    Cambridge, MA 02139 (US)
  • ESTES, Scott D.
    Cambridge, MA 02139 (US)
  • GOLDEN, Kathryn E.
    Cambridge, MA 02139 (US)
  • KONSTANTINOV, Konstantin
    Cambridge, MA 02140 (US)
  • WILLIAMS, Douglas E.
    Cambridge, MA 02139 (US)

(74) Representative: Carpmaels & Ransford LLP 
One Southampton Row
London WC1B 5HA
London WC1B 5HA (GB)


(56) References cited: : 
EP-A2- 2 450 032
WO-A2-2012/131733
US-A1- 2014 044 647
US-A1- 2016 113 966
US-A1- 2016 245 812
 WO-A1-2017/072744
US-A1- 2009 252 749
US-A1- 2014 234 263
US-A1- 2016 113 966
   
  • LOBB et al.: "Optimized Exosome Isolation Protocol for Cell Culture Supernatant and Human Plasma", Journal of Extracellular Vesicles, vol. 4, no. 27031, 17 July 2015 (2015-07-17), pages 1-11, XP055279331,
  • PACHLER et al.: "A Good Manufacturing Practice-Grade Standard Protocol for Exclusively Human Mesenchymal Stromal Cell -Derived Extracellular Vesicles", Cytotherapy, vol. 19, 1 April 2017 (2017-04-01), pages 458-472, XP055585278,
   
Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).